The nucleotide sequence of a streptomycin streptomycin phosphotransferase (streptomycin kinase) [corrected] gene from a streptomycin producer

The nucleotide sequence of the DNA fragment containing the streptomycin phosphotransferase (streptomycin kinase) [corrected] gene from the streptomycin-producer Streptomyces griseus strain HUT 6037 was determined. Analysis of the sequence revealed an open reading frame which could encode 325 amino acid residues. A biased codon usage pattern, reflecting the high G + C composition (approximately 74%) of Streptomyces DNA, was observed in the gene.     

Roles of streptomycin 6 -kinase and ribosomal affinity to streptomycin in self-protection of streptomycin producer

Summary In streptomycin (SM)-producing organisms, the lower affinity of ribosomes for SM gives rise to a lower susceptibility of protein synthesis to SM. But, even in a strain with considerably low affinity of ribosomes for SM, phosphorylation of SM in the cells by SM 6-kinase is necessary for the protein synthesis to be fully tolerant to SM.     

Kinetic studies of streptomycin uptake implicated in self-resistance in a streptomycin producer

Summary Streptomycin (SM)-producingStreptomyces griseus was permeable to extracellular SM during exponential growth, and less permeable during the stationary phase when antibiotic production was maximal. Uptake of [³H] dihydrostreptomycin ([³H]DSM) by the producer organism was abolished by inhibitors of electron transport, sulfhydryl reagents and an uncoupler of oxidative phosphorylation, and it was competitively inhibited by spermidine. These results indicate that SM was taken up by an active transport process via a polyamine transport system. A mutant with lower SM-resistance showed the same level of SM 6-phosphotransferase as the parent strain. It is suggested that selfresistance in the SM-producers is at least partly determined by transport and permeability mechanisms.