共查询到20条相似文献,搜索用时 31 毫秒
1.
Identification by Giemsa technique of the translocations separating cultivated rye from three wild species of Secale 总被引:1,自引:0,他引:1
Complete identification of the translocations involved in evolution of S. vavilovii, S. africanum and S. cereale from S. montanum was attained by meiotic analysis after Giemsa banding technique. Based on the original mitotic karyotype of S. montanum, the different chromosome arms were determined by centromere position and banding pattern of chromosomes for the four species and all of the possible interspecific hybrids. This first consistent scheme of cytogenetic relationships reveals: one translocation each, separating S. montanum from S. vavilovii and S. africanum, two translocations each, separating S. cereale from S. vavilovii and S. africanum, and three translocations each, separating S. cereale from S. montanum and S. africanum, respectively. 相似文献
2.
Howard C. Stutz 《American journal of botany》1972,59(1):59-70
From extensive cytological, ecological, and morphological studies, it appears that cultivated rye (Secale cereale L.) originated from weedy products derived from introgression of S. montanum into S. vavilovii. Secale vavilovii appears to have been derived from S. silvestre as a consequence of chromosomal translocations. Secale silvestre was, in turn, derived from S. montanum or a common ancestor. Secale africanum, S. dalmaticum, S. ciliatoglume, and S. kuprijanovii appear to be only slightly modified isolated populations of S. montanum. Populations of S. anatolicum are weedy forms of S. montanum, genealogically and chromosomally distinct from the weedy annual forms from which S. cereale arose. 相似文献
3.
Khush , G. S., and G. L. Stebbins . (U. California, Davis.) Cytogenetic and evolutionary studies in Secale. I. Some new data on the ancestry of S. cereale. Amer. Jour. Bot. 48(8): 723–730. Illus. 1961.—Cultivated rye, Secale cereale, was crossed with all 4 wild species of the genus. It crosses readily with S. montanum, S. africanum, and S. vavilovii, but crossability of S. cereale and S. silvestre is extremely low. Meiosis in the hybrid S. cereale × silvestre is characterized by high frequency of univalents at metaphase I, reduced chiasma frequency at metaphase I, high frequency of PMC's with unequal separations and laggards at anaphase I and II, high frequency of microspores with micronuclei, and extremely low pollen fertility. These abnormalities occurred less frequently in other hybrids, and consequently the pollen fertility is fairly high: 19.1% in the hybrid S. cereale × vavilovii and 31.3% and 31.8%, respectively, in the hybrids S. cereale × montanum and S. cereale × africanum. An interesting cytogenetic feature of all these hybrids, however, was the formation of a translocation configuration of 6 chromosomes at metaphase I of meiosis. In the hybrid, S. cereale × silvestre, a translocation configuration of 8 chromosomes was observed in a few cells. It is evident, therefore, that the genome of S. cereale differs from the genomes of wild species by 2 translocations. Another small translocation may differentiate S. cereale and S. silvestre, in addition. Thus, on the basis of chromosome arrangements, no particular wild species is most likely to be ancestral to S. cereale. Similarly, Stutz's hypothesis of hybrid origin of S. cereale seems highly improbable. After considering ecological preferences, breeding habits, geographical distribution, morphological and cytological affinities of wild species and cultivated rye, it is concluded that S. cereale evolved from S. montanum as a result of progressive cytological and morphological differentiation and that this differentiation was facilitated, probably, by adaptive superiority of translocation heterozygotes and rearrangement homozygotes. 相似文献
4.
The genetic frequencies of 9 isozyme loci have been estimated in 23 samples of 4 species ofSecale by means of starch gel electrophoresis. The populations ofS. silvestre andS. vavilovii were monomorphic and uniform within each species, those ofS. montanum andS. cereale were polymorphic for most of the isozyme loci. On the basis of isozyme patterns as well as allelic and genotypic frequencies of isozyme loci,S. silvestre can be distinguished fromS. vavilovii, and both fromS. cereale andS. montanum; but there is no clear differentiation between the two latter species. Clusters constructed from genetic distances separateS. silvestre andS. vavilovii, whereasS. cereale andS. montanum were grouped together. The isozymatic data presented here, along with cytogenetic and life habit data, agree with the generally admitted existence of 4 species inSecale, and support the relationships suggested byKhush & Stebbins (1961). 相似文献
5.
Meiotic configurations and segregation were studied in tetraploid hybrids (4n=28) between Secale cereale (2n=14) carrying a reciprocal translocation between chromosomes II and VI and a derivative of Secale montanum (2n=14), naturally differing from S.cereale in a translocation involving chromosomes I, III and V. Multivalent frequencies of the translocation complexes were reduced compared to expected but of still appreciable magnitude, which was ascribed to incomplete preferential pairing. Marker segregation (one of the S. cereale translocation chromosomes) also suggested partial preferential pairing. 相似文献
6.
J. Sybenga 《Genetica》1973,44(2):270-282
Chromosomal segregation, and the frequency of large multivalents in Secale cereale were studied in autotetraploid duplex translocation heterozygotes. Models for estimating expected segregations and frequencies of multivalents were developed incorporating the probabilities of different chromosomal segments being bound by chiasmata. It appeared that the segregation of the two translocations tested fitted quite well the expected corrected segregation ratio of approximately 1: 11.5: 1, suggesting that induced preferential pairing was not strong enough to enhance preferential segregation resulting from random translocation segregation. Interspecific hybrids with S. montanum carrying the same translocations showed strong preferential pairing, i.e. significant deviation from the expected ratios.Three translocations tested (two not tested for segregation) showed a decrease in multivalent frequency mainly attributable to preferential pairing, especially in cases where the breakpoint was near one chromosome end. Possible reasons why preferential pairing is expressed here and not in the segregations are discussed. 相似文献
7.
Variation in nuclear DNA in the genus Secale 总被引:2,自引:0,他引:2
Estimates of the 4C DNA amount per nucleus in 16 taxa of the genus Secale made by Feulgen microdensitometry ranged from 28.85 picograms (pg) in S. silvestre PBI R52 to 34.58 pg in S. vavilovii UM 2D49, compared with 33.14 pg in S. cereale cv. Petkus Spring which was used as a standard. Giemsa C-banding patterns showed considerable interspecific and intraspecific variation and several instances of polymorphism for large telomeric C-bands. The proportion of telomeric heterochromatin in the genome ranged from about 6% in S. silvestre and S. africanum to about 12% in cultivated rye. A detailed comparison of nine taxa showed no overall relationship between 4C DNA amount and the proportion of telomeric heterochromatin in the genome. However, evidence is presented which strongly supports the notion that the major evolutionary change in chromosome structure in Secale has involved the addition of heterochromatin at, or close to, the telomeres. It is suggested that saltatory amplification events at telomeres were initially responsible for each large increase in DNA amount. Subsequently unequal crossing over between homologues may have played an important secondary role by extending the range of variation in the amount of heterochromatin at a given telomere, while crossing over between non-homologues may have provided a useful mechanism allowing an increase in the DNA amount at one telomere to be distributed between chromosomes. 相似文献
8.
The structure, copy number and chromosomal location of arrays of four families of highly repeated sequences have been investigated in representative species of the genus Secale. The four unrelated families, previously characterised in Secale cereale, have repeating units of 480, 610, 630 and 120 base pairs respectively. The following general conclusions can be drawn in addition to detailed knowledge of the sequence content of heterochromatin in each accession studied: (1) Every species is unique in its complement or chromosomal distribution or both of the four highly repeated sequence families. S. montanum and S. cereale accessions studied here show the same complement of repeated sequences, but they differ substantially in the amounts they contain of the 610 and 630 base pair (bp) families, and in the distribution over the chromosomes of the 480 bp family. The structure of the repeating unit is also different in many members of the 480 bp family in S. montanum. — (2) The substantial differences between species in the amounts of the most highly repeated DNA sequences exist in the absence of any such conspicuous differences in most other repeated sequences which were detected as fluorescent bands after restriction enzyme digestion and gel electrophoresis. — (3) Each of the different highly repeated families can exist independently of the other families, though all the families have telomeric sites. Also, in the outbreeding species, heteromorphisms are frequent, and are particularly conspicuous in hybridisation detecting the 480 bp sequence family. — (4) The association of the highly repeated sequences with heterochromatin, discussed in the accompanying paper is generally true for other species in the genus, and the lower amounts of heterochromatin in other Secale species compared to S. cereale are associated with lower amounts of specific families of highly repeated DNA sequences. — (5) Analysis of highly repeated sequence families is likely to provide an easy method of identification of new accessions of Secale. 相似文献
9.
P. K. Gupta 《Genetica》1971,42(2):199-213
The work on methods for determining the homoeologous relationship between wheat and rye chromosomes has been reviewed. The results obtained for rye chromosomes belonging to different homoeologous groups have been discussed. It is proposed that chromosome 3R of Lee et al. (1969) should be designated as 1R/3R. It is pointed out that homoeology of all seven rye chromosomes may not be known in the future also, due to translocations. It is, therefore, suggested that Secale montanum should be used instead of S. cereale. Future lines of work have been suggested. 相似文献
10.
Carmona R. Puertas M. J. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1977,51(3):111-117
Summary The meiotic behaviour of hybrids between Secale cereale carrying B chromosomes and S. vavilovii has been studied in order to estimate the effects of B chromosomes on hybrid meiotic pairing. The possible effect of Bs on the meiotic pairing of the offspring from backcrosses with S. vavilovii has been studied also. The results obtained clearly indicate that no detectable differences existed in chromosome pairing of hybrids with or without B chromosomes. The hypothetical existence of epistatic genes on cereale genome masking the effect of Bs has been rejected after the results obtained in backcrosses. Therefore, lack of qualitative genes controlling interspecific pairing on rye B chromosomes has been concluded. A quantitative effect of B chromosomes was detected only when they were in alien cytoplasm. 相似文献
11.
C. Benito F. J. Gallego C. Zaragoza J. M. Frade A. M. Figueiras 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,82(1):27-32
Summary The segregation of different isozymic loci was investigated in backcrosses and F2s in rye. The leucin aminopeptidase-1 (Lap-1), Aconitase-1 (Aco-1), Esterase-6 (Est-6), Esterase-8 (Est-8), and Endopeptidase-1 (Ep-1) loci were linked. The Aco-1, Est-6, and Est-8 loci have been previously located on the 6RL chromosome arm. The Lap-1 locus has been located on the 6RS chromosome arm. The results favor the gene order: Lap-1... (centromere)... Aco-1... Est-8... Est-6... Ep-1. The isoelectric focusing separations of aqueous extracts from mature embryo tissue of wheat-rye addition and substitution lines involving the chromosomes of cereal rye Secale cereale L. confirmed the gene location of locus Ep-1 on the 6RL chromosome arm. Screening of wheat-rye addition lines involving the chromosomes of Secale montanum revealed that Ep-1 locus is not located on chromosome 6R of S. montanum. These results are the first biochemical evidence of the translocation between chromosome arms 6RL/7RL in the evolution of S. cereale from S. montanum. 相似文献
12.
P. Wehling 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,82(5):569-576
Summary Genetic analysis of the inheritance of glutamate oxaloacetate transaminase (GOT), acid phosphatase (ACP) and diaphorase (DIA) in leaf tissue of rye revealed the involvement of four Got, four Acp and three Dia loci. Linkage analysis led to the arrangement of these and other previously described isozyme loci into four linkage groups located on chromosomes 3Rq, 4Rq, 6R, and 7Rq, respectively. Implications of these findings for possible translocation differences between chromosomes of S. cereale and S. montanum are discussed. A
2 component analysis which makes use of the entire potential of the information provided by codominantly inherited traits such as isozymes is described. 相似文献
13.
Identification of sterility-inducing cytoplasms in rye using the plasmotype–genotype interaction test and newly developed SCAR markers 总被引:1,自引:0,他引:1
Stojałowski S Lapiński M Szklarczyk M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(4):627-633
A series of 25 rye (Secale cereale L.) inbred lines was tested with respect to three mitochondrial sequence-characterized amplified region (SCAR) polymorphisms.
The analysis revealed a close association between the marker-determined mitotypes and plasmotypes (cytoplasm types known from
breeding data) represented by the inbreds. The mitochondrial markers also confirmed normal (N-) cytoplasmic character of three
wild rye species: Secale montanum, S. vavilovii and S. kuprijanovii. For 186 plants from open-pollinated cultivars of Turkish and South American origin, cytoplasm identification was performed
with the use of crossing with double non-restoring tester. In 77 plants the normal (N) cytoplasm was detected, and for 63
of these the PCR analysis was performed producing results which were consistent with the genetic data based on testcrossing
and phenotype assessment. The mitochondrial markers also confirmed a presence of sterility-inducing cytoplasm in the remaining
109 plants. Moreover, the markers allowed for differentiation between Pampa (P-) and Vavilovii (V-) cytoplasmic individuals.
For 60 plants the latter results were verified using crosses with a line maintaining P-cytoplasmic sterility and acting as
a restorer of the V-cytoplasm. For two of these plants contradicting results were produced with the applied methods of cytoplasm
identification and the basis of this discrepancy is discussed. Regardless of the identification method, widespread occurrence
of a sterility-inducing cytoplasm was revealed, especially in South American populations. 相似文献
14.
P. Garcia Dr. M. Pérez de la Vega C. Benito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,61(4):341-351
Summary Genetic analyses were conducted on peroxidase of the embryo and endosperm of seeds of one open pollinated and six inbred lines of cultivated rye (Secale cereale L.), and one line of Secale vavilovii Grossh. The analyses of the individual parts of the S. cereale seed yield a total of 14 peroxidase isozymes. Isozymes m, a, b, c, d, e, f and g (in order from faster to slower migration) were found in the embryo plus scutellum, while isozymes 1, 2, 3, 4, 5 and 6 (also from faster to slower migration) were peculiar of the endosperm. S. vavilovii has isozymes m, c1, d, e, f and g in its embryo plus scutellum, and isozyme 2 in the endosperm. Segregation data indicated that at least 13 different loci would be controlling the peroxidase of S. cereale. Isozymes a and b are controlled by alleles of the same locus, all the other loci have one active and dominant allele coding for one isozyme, and other null and recessive allele. The estimation of linkage relationships shows that five endosperm loci are linked, and tentative maps are shown. A possible dosage effect and the existence of controlling gene(s) for endosperm isozyme 4 is reported. All these data and the high frequency of null alleles found are discussed in relation to recent reports. 相似文献
15.
The difficulties encountered in classifying the seven chromosomes of rye (Secale cereale L.) are discussed. Unequivocal classification is possible only with a standard testing system such as a translocation tester set. In the present paper a set is described which contains seven reciprocal translocations. Each chromosome participates at least once. The translocated chromosomes can be visually recognized. The size of the chromosome arms was measured and is expressed as % of the total complement length. Which chromosomes were involved in the translocations was studied by using a special graphic method based on the arm ratios of the mitotic chromosomes, and also by intercrossing followed by an analysis of the meiotic cofigurations in the F1's. 相似文献
16.
Proteins from pollen of parent forms and amphiploids Aegilops
variabilis ×Secale cereale and Ae. kotschyi×S. cereale, obtained by in vitro propagation or colchicine treatment of F1 hybrids, were subjected to a study by two-dimensional (2-D) electrophoresis. Qualitative and quantitative diversities of
protein patterns were revealed for the amphiploid pollen. The majority of peptides found in the parent forms were also present
in the patterns of the amphiploid pollen; however, some of the parent-form-peptides were not expressed and proteins characteristic
only of the amphiploids appeared. In the 2-D combined protein pattern obtained for the parent forms, amphiploids Ae. variabilis × S. cereale produced pollen with a poorer spectrum of proteins. In amphiploid 408B, obtained from treated the F1 generation with colchicine, the 2-D pattern revealed the presence of less than 50% of the proteins recorded for the parent
forms. Pollen grain morphology was studied under a scanning microscope. The structure and shape of exines differed from those
of the parents. In the parent forms the pollen grains had only one pore, while in amphiploid pollen, one, two or three pores
were observed. Possible explanations for the differences in the 2-D patterns of amphiploids and their parent forms (impoverishment
of the protein spectrum and appearance of new peptides) are (1) somaclonal variation and mutagenic activity of colchicine,
(2) suppression of structural genes, (3) activity of regulators and (4) translocations. Pollen grains with two or even three
pores could appear as a result of the independent activity of the genes from three amphiploidal genomes.
Received: 6 March 2001 / Accepted: 26 June 2001 相似文献
17.
Ermishev V. Yu. Naroditsky B. S. Khavkin E. E. 《Russian Journal of Plant Physiology》2002,49(5):657-664
The fragments of MADS-box genes belonging to the agamous and agamous-like structural classes were isolated by direct amplification of genomic DNA from annual rye (Secale cereale L.) and perennial rye (Secale montanum Guss.). The characterized fragments (deposited in the Genbank as the accession nos. AF332885–AF332887 and AF346894) comprise the complete sequences of exons 1 to 5 and lack corresponding introns. Their nearest homologs are the maize genes zag1 and zag5 (the Genbank accession nos. L18924 and L46398). One more agamous-like fragment isolated from annual rye (AF362364) is similar to theTaMADS12 wheat gene (AB007505). The fragment comprises exons 3–5 and contains a 105-bp insert between the exons 3 and 4; this insert does not resemble any MADS-box introns presently known. We assume that all these fragments of MADS-box genes are retropseudogenes. 相似文献
18.
Książczyk T Apolinarska B Kulak-Książczyk S Wiśniewska H Stojałowski S Łapiński M 《Journal of applied genetics》2011,52(3):305-311
Genome modifications that occur at the initial interspecific hybridization event are dynamic and can be consolidated during
the process of stabilization in successive generations of allopolyploids. This study identifies the number and chromosomal
location of ribosomal DNA (rDNA) sites between Secale cereale, Dasypyrum villosum, and their allotetraploid S. cereale × D. villosum hybrids. For the first time, we show the advantages of FISH to reveal chromosome rearrangements in the tetraploid Secale × Dasypyrum hybrids. Based on the specific hybridization patterns of ribosomal 5S, 35S DNA and rye species-specific pSc200 DNA probes,
a set of genotypes with numerous Secale/Dasypyrum translocations of 1R/1V chromosomes were identified in successive generations of allotetraploid S. cereale × D. villosum hybrids. In addition we analyse rye chromosome pairs using FISH with chromosome-specific DNA sequences on S. cereale × D. villosum hybrids. 相似文献
19.
B. R. Lu R. von Bothmer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1991,81(4):524-532
Summary Integeneric hybridizations were carried out between Secale cereale L. (2n = 14, RR) and three Elymus species, namely, E. caninus (L.) L. (2n = 28, SSHH), E. brevipes (Keng) Löve (2n = 28, SSYY) and E. tsukushiensis Honda (2n = 42, SSHHYY). Chromosome pairing was studied at metaphase I in the parental species and the hybrids. Meiotic configurations of the hybrids were 20.74 1+0.14 II for E. caninus x S. cereale (SHR), 16.35 I+2.17 II+0.09 III for E. brevipes x S. cereale (SYR) and 25.84 I+1.10 II+0.02 III for E. tsukushiensis x S. cereale (SHYR), in addition to some secondary associations in the different hybrids. It is concluded from the study that (1) a certain, different homoeologous relationship exists among S, H and Y genomes in the investigated Elymus species; (2) low homoeology is present between genomes of Elymus (S or H or Y) and rye (R); (3) the Secale genome affects homoeologous chromosome pairing between different genomes in E. brevipes and E. tsukushiensis. 相似文献
20.
Eva Bauer Thomas Schmutzer Ivan Barilar Martin Mascher Heidrun Gundlach Mihaela M. Martis Sven O. Twardziok Bernd Hackauf Andres Gordillo Peer Wilde Malthe Schmidt Viktor Korzun Klaus F.X. Mayer Karl Schmid Chris‐Carolin Schön Uwe Scholz 《The Plant journal : for cell and molecular biology》2017,89(5):853-869
We report on a whole‐genome draft sequence of rye (Secale cereale L.). Rye is a diploid Triticeae species closely related to wheat and barley, and an important crop for food and feed in Central and Eastern Europe. Through whole‐genome shotgun sequencing of the 7.9‐Gbp genome of the winter rye inbred line Lo7 we obtained a de novo assembly represented by 1.29 million scaffolds covering a total length of 2.8 Gbp. Our reference sequence represents nearly the entire low‐copy portion of the rye genome. This genome assembly was used to predict 27 784 rye gene models based on homology to sequenced grass genomes. Through resequencing of 10 rye inbred lines and one accession of the wild relative S. vavilovii, we discovered more than 90 million single nucleotide variants and short insertions/deletions in the rye genome. From these variants, we developed the high‐density Rye600k genotyping array with 600 843 markers, which enabled anchoring the sequence contigs along a high‐density genetic map and establishing a synteny‐based virtual gene order. Genotyping data were used to characterize the diversity of rye breeding pools and genetic resources, and to obtain a genome‐wide map of selection signals differentiating the divergent gene pools. This rye whole‐genome sequence closes a gap in Triticeae genome research, and will be highly valuable for comparative genomics, functional studies and genome‐based breeding in rye. 相似文献