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1.
Mutant strains of the unicellular cyanobacterium Synechocystissp. PCC 6803, in which the psaK gene was insertionally inactivatedby targeted mutagenesis, were constructed. The gene is one ofthe two potential PsaK-coding genes which have been found asa result of the genome project with this cyanobacterium. Oneof the mutants was characterized in detail. A monocistronic,480-nucleotide mRNA of psaK was absent in total RNA from themutant cells. Inactivation of psaK had little effect on theaccumulation of polypeptides in the isolated PSI complexes exceptfor a polypeptide with an apparent molecular mass of 4.6 kDawhich was absent in the mutant. The amino-terminal amino acidsequence of the 4.6-kDa polypeptide confirmed that it was thetranslation product of psaK and further revealed a presequenceof PsaK. Characteristics of photoautotrophic growth at differenttemperatures, the amount of chlorophyll per cell, photosyntheticelectron transport rates with various electron acceptors, thekinetics of charge recombination between P700+ and reduced FA/FB,and the molar ratio of chlorophyll to P700, of the mutant werenot significantly different from those of the wild type. Furthermore,the trimer to monomer ratio of the PSI complexes isolated fromthe mutant was similar to that isolated from the wild type. (Received July 27, 1998; Accepted October 13, 1998) 相似文献
2.
Changes in intracellular levels of Chl a precursors were examinedin relation to changes in the PSI/PSII stoichiometry in thecyanophyte Synechocystis PCC 6714. Protochlorophyllide (Pchlide)accumulated markedly in cells with a low PSI/PSII stoichiometrygrown under light that is absorbed by Chl a (PSI light) whereasno accumulation occurred in cells with a high PSI/PSII stoichiometrygrown under light absorbed by phycobilisomes (PSII light). Levelsof Pchlide in cells grown under PSI light decreased rapidlyupon a shift to PSII light. The rapid decrease in Pchlide accompanieda transient increase in chlorophyllide a, indicating that reductionof Pchlide was enhanced by shift to PSII light. The action spectrumindicated that the Pchlide decrease upon the shift to PSII lightdepended on excitation of Pchlide, suggesting that the accumulationof Pchllide was due to limited excitation of Pchlide, so thatPchlide photoreduction, under PSI light. However, comparisonof levels of Pchlide and the photosystem complexes in wild-typePlectonema boryanum with those in a mutant that lacked the darkPchlide reductase (YFC 1004) indicated that dark reduction compensatedfor the limited photoreduction under PSI light. Similar compensationby dark reduction was confirmed with Synechocystis PCC 6714.In cultures of Synechocystis under conditions where Pchlidecould not be photoreduced, accumulation of Pchlide and low PSI/PSIIstoichiometry occurred only when cells were illuminated withlight that preferentially excited PSI. The results indicatethat the low PSI/PSII stoichiometry in cells grown under PSIlight is not a result of inefficient synthesis of Chl a witha reduced rate of Pchlide photoreduction. They suggest furtherthat accumulation of Pchlide under PSI light results from retardationof the Chl a synthesis due to suppression of PSI synthesis.
1Present address: Tsurukawa 5-15-11, Machida, Tokyo, 195 Japan. 相似文献
3.
Exposure of the Cyanobacterium Synechocystis PCC6803 to Salt Stress Induces Concerted Changes in Respiration and Photosynthesis 总被引:8,自引:0,他引:8
Jeanjean Robert; Matthijs Hans C.P.; Onana Boyomo; Havaux Michel; Joset Franoise 《Plant & cell physiology》1993,34(7):1073-1079
In order to survive and to grow in the presence of a high salinity(550 mM NaCl) Synechocystis PCC6803 increases its energeticcapacity. The salt-induced increase of electron transport ratesinvolves both cytochrome c oxidase and photosystem I. In contrast,electron transport rates measured through complexes I plus IIIof the respiratory chain, or through the photosystem II pluscytochrome b6f complexes of the photosynthetic chain, do notshow appreciable changes. The time at which changes in electrontransport rates occur in the photosystem I and cytochrome coxidase complexes after the onset of salt stress indicates similaritiesin the adaptation of dark respiration and (cyclic) photosyntheticelectron flow. Given an increase of whole cell respiration andof PSI cyclic electron flow larger than the neosynthesis ofcytochrome aa3 and PSI reaction centers would predict, it appearsthat both adaptations require more than just synthesis of thesetwo complexes. (Received April 12, 1993; Accepted August 10, 1993) 相似文献
4.
5.
Abe Shin-ichiro; Murakami Akio; Ohki Kaori; Aruga Yusho; Fujita Yoshihiko 《Plant & cell physiology》1994,35(6):901-906
Stoichiometry among 3 thylakoid components, PSI and PSII andCyt b6-f complexes, was determined with the red alga Porphyrayezoensis with special reference to the regulation of PSI/PSIIstoichiometry in response to light regime. The ratio of PSIto PSII abundance was four times greater in thalli grown underorange light which excites mainly phycobilisome, thus PSII,than that under red light which excites preferentially Chl a,thus PSI. Cyt b6-f abundance remained almost constant. The PSIand PSII content was regulated separately under the two growthlight conditions as was also observed with the red alga Porphyridiumcruentum by Cunningham et al. [(1990) Plant Physiol. 93: 888].This differs from the cyanophyte Synechocystis PCC 6714 whereadjustment occurs only in the PSI content [(1987) Plant CellPhysiol. 28: 1547]. However, results on the marine cyanophyteSynechococcus NIBB 1071 indicate that changes in the PSI/PSIIsoichiometry is similar to red algae. In this species, as inthe red algae, more than one PSII is associated with each phycobilisome.The light regime also induced changes in the phycobiliproteincomposition in Porphyra yezoensis. Under PSII light, phycoerythrinincreased, and phycocyanin decreased, while under PSI lightthe response was reversed. The change suggests an occurrenceof complementary chromatic adaptation. (Received April 8, 1994; Accepted June 1, 1994) 相似文献
6.
The effect of the Cyt b6-f redox state on the PSI formationwas examined with the cyanophyte Synechocystis PCC 6714 by usinga Q-cycle inhibitor, HQNO (2-n-heptyl-4-hydroxyquinoline N-oxide).HQNO inhibited the rapid reduction of flash-oxidized Cyt f,the reaction correlating with the stimulation of PSI formation,on one hand, and accumulated reduced Cyt b6, on the other, indicatingthat the electron flow in the Q-cycle correlates with regulationof PSI synthesis. HQNO also inhibited the stimulation of PSIformation under PSII light, resulting in a low PSI/PSII ratioeven under PSII light, while the PSI formation under PSI lightwas not suppressed by HQNO. Simultaneous inhibition of Cyt b6oxidation through the Q-cycle and the stimulated PSI formationby HQNO suggests that an HQNO-sensitive Cyt b6 oxidation isinvolved in the mechanism of monitoring the state of electrontransport system for regulation of PSI formation. (Received March 3, 1993; Accepted August 9, 1993) 相似文献
7.
Murakami Akio; Fujita Yoshihiko; Nemson Jeff A.; Melis Anastasios 《Plant & cell physiology》1997,38(2):188-193
Photosystem stoichiometry adjustments in Chlamydomonas reinhardtiiwere induced upon a sudden shift in the light quality duringcell growth. Reversible changes in the PSI/PSII ratio were acompensation response to changes in the balance of light absorptionby the two photosystems. Quantitations of PSII, Cyt b6-f complexand PSI revealed a constancy in the cellular content of PSIIand the Cyt b6-f complex, and variable amounts of PSI in C.reinhardtii. These results strengthen the notion that PSI isthe thyla-koid component subject to chromatic regulation andresponsible for the adjustment and optimization of the PSI/PSII ratio in the thylakoid of oxygenic photosynthesis. Additionalresults, obtained upon the use of protein biosynthesis translationinhibitors (chloramphenicol and cyclohex-imide), suggested thata chromatically-induced lowering of the PSI/PSII ratio in C.reinhardtii occurs by suppression of de novo biosynthesis ofPSI components and, therefore, by dilution of the PSI complexin the thylakoid membrane, rather than by active degradationof assembled PSI in chlo-roplasts. (Received November 8, 1996; Accepted December 6, 1996) 相似文献
8.
Compositions of pigments and polypeptides of pale green membranesthat had been isolated from dark-grown cells of a chlorophyll-deficientmutant of Chlorella kessleri were investigated. They containedChl a in a level corresponding to about 1% of that present inthe thylakoid membranes isolated from autotrophically grownwild-type cells and a trace amount of chlorophyllide a, butneither Chl b nor carotenoids. The polypeptide profile of themutant membranes was similar to that of membranes isolated fromwild-type cells that were grown in the dark. Neither the chlorophyll-bindingsubunits of PSI nor the apoproteins of LHCP were detected bySDS-PAGE and immunoblot analysis. However, the light-minus-darkdifference spectrum of the mutant membranes revealed the presenceof the reaction-center chlorophyll of PSI (P700) at a molarratio of 190 chlorophyll (Chl a plus Chlide a) per P700. P700was more stable than Chl a and Chlide a in the light so thatprolonged illumination led to a decline in the Chl/P700 ratioto 24. The initial rate of P700 photooxidation in the mutantmembranes was comparable to that in CP1 isolated from the dark-grownwild-type cells. Under illumination with strong light, the initialrate was decreased in parallel to the decrease in Chl/P700 ratio.The results suggest that most of Chi present in the mutant membranescan transfer excitation energy to P700. (Received March 13, 1998; Accepted August 7, 1998) 相似文献
9.
Gu Tian-Qing; Iwama Yumi; Murakami Akio; Adhikary Siba Prasad; Fujita Yoshihiko 《Plant & cell physiology》1994,35(8):1135-1140
Changes in the activity of cytochrome c oxidase (EC 1.9.3.1
[EC]
,Cyt-oxidase) in response to growth conditions were studied withthe cyanophyte Synechocystis PCC 6714 in relation to changesin PSI abundance induced by light regime for photosynthesis.The activity was determined with the Vmax of mammalian cytochromec oxidation by isolated membranes. The activity of glucose-6-phosphate(G-6-P):NADP+ oxidoreductase (EC 1.1.1.49
[EC]
) was also determinedsupplementarily. Cyt-oxidase activity was enhanced by glucoseadded to the medium even when cell growth maintained mainlyby oxygenic photosynthesis. G-6-P:NADP+ oxidoreductase was alsoactivated by glucose. The enhanced level of Cyt-oxidase washigher under PSII light, which causes high PSI abundance, thanthat under PSI light, which causes low PSI abundance. The levelwas intermediate under hetetrotrophic conditions. Although theactivity level was low in cells grown under autotrophic conditions,the level was again lower in cells grown under PSI light thanunder PSII light. The change of Cyt-oxidase activity in responseto light regime occurred in the same direction as that for thevariation of PSI abundance. Results suggest that in SynechocystisPCC 6714, the capacity of electron turnover at the two terminalcomponents of thylakoid electron transport system, Cyt-oxidaseand PSI, changes in parallel with each other in response tothe state of thylakoid electron transport system.
1Present address: Institute of Botany, Academia Sinica, Beijing100044, China
2Present address: Department of Botany, Utkal University, Bhubaneswar,India 751004 相似文献
10.
This study was done to confirm our previous observation withthe pattern of changes in electron transport composition inducedby an imbalance of the electron transport state. Contents ofphotosystem (PS) I and II complexes and their antennae and Cytb6/f complex were determined for systems of cyanobacterium SynechocystisPCC 6714 of different PS I/PS II ratios. The results indicatedthat (1) the observed changes in the PS I/PS II ratio are not-dueto regulation of the activities of the respective PS's but tochanges in their contents, (2) the molar ratio between PS IIand Cyt b6/f complexes was fairly constant when marked changesoccurred in the PS I content, and (3) the PS II and Cyt b6/fcontents per cell remained fairly constant while the PS I contentchanged markedly. These findings agree with our previous observationwith autotrophic cells of Anacystis nidulans Tx 20 and supportour argument that in cyanobacterial and red algal electron transportsystems, the content of the terminalcomponent(s), such as PSI complex, is regulated in order to maintain a balance betweenthe electron influx by PS II action to the system and the effluxby PS I action from it. (Received June 3, 1987; Accepted September 20, 1987) 相似文献
11.
Tobacco Nuclear Genes for Photosystem I Subunits, psaD, psaE and psaH Share an Octamer Motif Bound with Nuclear Proteins 总被引:1,自引:0,他引:1
In Nicotiana sylvestris, nuclear-encoded photosystem I (PSI)genes, psaD, psaE and psaH, share an octamer motif bound withthree phosphoproteins. This motif is not found in the chloroplastgenome. From the view point of endosymbiont hypothesis, theseresults suggest that a set of ancient PSI genes acquired a commoncis-element in the nucleus after they were transferred fromthe ancestral organelle. (Received March 20, 1995; Accepted August 9, 1995) 相似文献
12.
Terao Tomio; Sonoike Kintake; Yamazaki Jun-ya; Kamimura Yasumaro; Katoh Sakae 《Plant & cell physiology》1996,37(3):299-306
Stoichiometries of photosystem I (PSI) and photosystem II (PSII)reaction centers in a cultivar of rice, Norin No. 8, and threechlorophyll b-deficient mutants derived from the cultivar wereinvestigated. Quantitation of PSI by photooxidation of P-700and chromatographic assay of vitamin K1 showed that, on thebasis of chlorophyll, the mutants have higher concentrationsof PSI than the wildtype rice. Greater increases were observedin the PSII contents measured by photoreduction of QA, bindingof a radioactive herbicide and atomic absorption spectroscopyof Mn. Consequently, the PSII to PSI ratio increased from 1.11.3in the wild-type rice to 1.8 in chlorina 2, which contains noChl b, and to 2.03.3 in chlorina 11 and chlorina 14,which have chlorophyll a/b ratios of 9 and 13, respectively.Measurement of oxygen evolution with saturating single-turnoverflashes revealed that, whereas at most 20% of PSII centers areinactive in oxygen evolution in the wildtype rice, the non-functionalPSII centers amount to about 50% in the three mutant strains.The fluorescence induction kinetics was also analyzed to estimateproportions of the inactive PSII in the mutants. The data obtainedsuggest that plants have an ability to adjust the stoichiometryof the two photosystems and the functional organization of PSIIin response to the genetically induced deficiency of chlorophyllb. (Received July 29, 1994; Accepted February 7, 1996) 相似文献
13.
Ten rice chlorina mutants of Type I, which totally lack chlorophyllb and hence are unable to synthesize light-harvesting chlorophylla/b protein complexes of photosystem II (LHC-II), containedmRNA for proteins related to LHC-II. Immunoblotting with anantiserum, which had been raised against the 24 and 25 kDa apoproteinsof LHC-II and found to cross-react with the 26 kDa protein ofLHC-II and the 20 and 21 kDa apoproteins of light-harvestingchlorophyll a/b protein complexes of photosystem I (LHC-I),revealed that all the five proteins related to LHC-Iand LHC-IIwere present in reduced amounts in the Type I mutants. ThreeType HA mutants, which have a chlorophyll a/b ratio of 10, weremore abundant in the apoproteins, while three Type IIB mutantswith the ratio of 15 were heterogeneous in terms of the apoproteincontent. All the chlorina mutants contained less P700 comparedwith the wild type rice, but were relatively more abundant inthe LHC-I proteins than the LHC-II proteins. The results showthat all the rice chlorina strains are mutants of chlorophyllb synthesis and the deficiency of chlorophyll b differentlyaffects accumulation of the apoproteins of LHC-I and LHC-II.To balance light absorption between the two photosystem, lossof LHC-II is partly counter-balanced by a decrease in the numberof PSI complexes in the mutants. (Received January 21, 1988; Accepted April 28, 1988) 相似文献
14.
Changes in photosystem stoichiometry in response to shift ofenvironments for cell growth other than light regime were studiedwith the cyanophyte Synechocystis PCC 6714 in relation to thechange induced by light-quality shift. Following two environment-shiftswere examined: the shift of molecular form of inorganic carbonsource for photosynthesis from CO2 to HCO3 (CO2 stress)and the increase in salinity of the medium with NaCl (0.5 M)(Na+ stress). Both CO2 and Na+ stresses induced the increasein PSI abundance resulting in a higher PSI/PSII stoichiometry.CO2 stress was found to elevate simultaneously Cyt c oxidaseactivity (Vmax). The feature was the same as that caused bylight-quality shift from preferential excitation of PSI to PSII(light stress) though the enhancement by either stress was smallerthan that by light stress. Under our experimental conditions,PSI/PSII stoichiometry appeared to increase at a fairly constantrate to the basal level even when the basal level had been differentlydetermined by the light-quality. Enhancing rates for PSI/PSIIstoichiometry and for Cyt c oxidase activity were also similarto each other. Since the two stresses affect the thylakoid electrontransport similarly to the shift of light-quality, we interpretedour results as follows: three environmental stresses, CO2, Na+,and light stresses, cause changes in electron turnover capacityof PSI and Cyt c oxidase under a similar, probably a common,mechanism for monitoring redox state of thylakoid electron transportsystem.
1On leave from Department of Biology, College of Natural Science,Kyngpook National University, Taegu 702-701, Korea.
2Present address: Department of Marine Bioscience, Fukui Pre-fecturalUniversity, Obama, Fukui, 917 Japan. 相似文献
15.
We have determined the complete nucleotide sequence of pAQ1,the smallest plasmid of the unicellular marine cyanobacteriumSynechococcus sp. PCC7002. The plasmid consists of 4,809 bpand has at least four open reading frames that potentially encodepolypeptides of 50 or more amino acids. We found that a palindromicelement, the core sequence of which is G(G/A)CGATCGCC, is over-representednot only in plasmid pAQ1 but also in the accumulated cyanobacterialgenomic sequences from Synechococcus sp. PCC6301, PCC7002, PCC7942,vulcanus and Synechocystis sp. PCC6803 within GenBank and EMBLdatabases. It suggests that this sequence might mediate generearrangement, thus increasing genetic diversity, since recombinationevents are frequent in cyanobacteria. 相似文献
16.
Farineau Nicole; Guillot-Salomon Th?r?se; Cantrel Catherine; Ouijja Abderrahman; Tuquet Christiane 《Plant & cell physiology》1988,29(6):925-933
Greening of etiolated seedlings of wild and Chl b-less barley(Hordeum vulgare L.) genotypes in the presence of D-threochloramphenicol(CAP) led to macrogranal arrangements accompanying the inhibitionof Chl synthesis and an enhancement of the total protein contentin differentiated etiochloroplasts. In treated mutant plastids,protein/Chl ratio reached up to 100. No light-dependent O2 evolution was detected in CAP-treatedplastids which had deficiency in polypeptides belonging to thephotosystem II (PSII) centres. On the other hand, plastids displayeda high photosystem I (PSI) activity despite the absence of the92 kDa polypeptide linked to the PSI centre. The accumulationof polypeptides ranging from 16 to 20 kDa suggest that theycould originate from primary complexes consisting of few Chlmolecules, but they were sufficient to allow the activity ofthe reaction centres. No accumulation of the 2527 kDapolypeptides linked to the PSII antenna was detected. The increase in the proportion of trans-3hexadecenoic acid (16:1tr) in phosphatidylglycerol (PG) of etiochloroplasts from bothtypes after CAP treatment could indicate an alteration of theregulation process of 16:1 tr biosynthesis occurring in plastids.The formation of macrograna could optimize the energy transferin altered thylakoid membranes. The accumulation of PG-16:1tr molecules could be related to the formation of active primarycomplexes in thylakoid when Chl synthesis is altered. (Received March 30, 1988; Accepted June 1, 1988) 相似文献
17.
A desiccation-tolerant cyanobacterium, Nostoc commune, showsunique responses to dehydration. These responses are: (i) lossof PSII activity in parallel with the loss of photosynthesis;(ii) loss of PSI activity; and (iii) dissipation of light energyabsorbed by pigment–protein complexes. In this study,the deactivation of PSII is shown to be important in avoidingphotoinhibition when the Calvin–Benson cycle is repressedby dehydration. Furthermore, our evidence suggests that dissipationof light energy absorbed by PSII blocks photoinhibition understrong light in dehydrated states. 相似文献
18.
Continuous wetness of leaves in the light causes a reductionin the carbon exchange rate (CER) in Phaseolus vulgaris L. [Ishibashiand Terashima (1995) Plant Cell Environ. 18: 431]. In this study,we investigated the initial cause of photoinhibition upon applicationof water, designated rain treatment, and we found a large decreasein the rate of electron transport through the whole chain fromwater to methyl viologen via PSII and PSI. In spite of the decreasein the rate of electron transport, there was no decrease inthe activity of either PSI or PSII when these activities weremeasured separately. The intactness of PSI was also confirmedby the absence of any change in the pho-tooxidizable amountof P-700, the reaction centre of PSI, and the intactness ofPSII was confirmed by measurements of Chi fluorescence. Theresults suggest that the inhibition by the rain treatment, whichoccurs at the site between PSI and PSII, might be a novel typeof photoinhibition, unlike the conventional types of photoinhibitionthat involve PSI and PSII. (Received July 29, 1996; Accepted November 28, 1996) 相似文献
19.
Kanervo E Singh M Suorsa M Paakkarinen V Aro E Battchikova N Aro EM 《Plant & cell physiology》2008,49(3):396-410
The protein complexes of pea (Pisum sativum L.) etioplasts,etio-chloroplasts and chloroplasts were examined using 2D BlueNative/SDS–PAGE. The most prominent protein complexesin etioplasts were the ATPase and the Clp and FtsH proteasecomplexes which probably have a crucial role in the biogenesisof etioplasts and chloroplasts. Also the cytochrome b6f (Cytb6f) complex was assembled in the etioplast membrane, as wellas Rubisco, at least partially, in the stroma. These complexesare composed of proteins encoded by both the plastid and nucleargenomes, indicating that a functional cross-talk exists betweenpea etioplasts and the nucleus. In contrast, the proteins andprotein complexes that bind chlorophyll, with the PetD subunitand the entire Cyt b6f complex as an exception, did not accumulatein etioplasts. Nevertheless, some PSII core components suchas PsbE and the luminal oxygen-evolvong complex (OEC) proteinsPsbO and PsbP accumulated efficiently in etioplasts. After 6h de-etiolation, a complete PSII core complex appeared with40% of the maximal photochemical efficiency, but a fully functionalPSII was recorded only after 24 h illumination. Similarly, thecore complex of PSI was assembled after 6 h illumination, whereasthe PSI–light-harvesting complex I was stably assembledonly in chloroplasts illuminated for 24 h. Moreover, a batteryof proteins responsible for defense against oxidative stressaccumulated particularly in etioplasts, including the stromaland thylakoidal forms of ascorbate peroxidase, glutathione reductaseand PsbS. 相似文献
20.
Regulation of the assembly of the photosystem I (PS I) complexin response to the light regime in the photosynthetic systemof cyanophytes was studied in Synechocystis PCC 6714. The relationshipbetween the assembly of the PS I complex and synthesis of Chla was examined by model experiments in which synthesis of Chla was controlled by two inhibitors, gabaculine (GAB) and 2,2'-dipyridyl(DP). Both inhibitors caused a change to a lower ratio of PSI to PS II even under light that normally induces a high ratioof PS I to PS II. The change in stoichiometry induced by theseinhibitors was suppressed when protein synthesis was inhibitedby chloram-phenicol, similarly to the change in the stoichiometryinduced by light that excites mainly PS I (PS I light). Comparisonof the levels of PS I, PS II and Cyt b6-f complexes per cellindicated that a selective suppression of the assembly of thePS I complex was induced by the inhibitors: the stoichiometricrelationship among PS I, PS II and Cyt b6-f complexes was identicalto that induced by PS I light or white light of high intensity.GAB induced a decrease in size of the phycobilisome also, whileDP did not, similarly to PS I light. The results indicate thatthe ratio of PS I to PS II can be changed by the control ofsynthesis of Chl a. They also suggest that control of the synthesisor supply of Chl a probably exerted at site(s) in or after theprocess of the Mg-protoporphyrin branch, is involved in themechanism of regulation of the assembly of the PS I complexin cyanophytes. (Received September 7, 1989; Accepted November 20, 1989) 相似文献