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1.
Label‐free and non‐invasive monitoring of porcine trophoblast derived cells: differentiation in serum and serum‐free media 下载免费PDF全文
Qifei Li Edison Suasnavas Lifu Xiao Sierra Heywood Xiaojun Qi Anhong Zhou S. Clay Isom 《Journal of biophotonics》2015,8(8):638-645
Traditional approaches to characterize stem cell differentiation are time‐consuming, lengthy and invasive. Here, Raman microspectroscopy (RM) and atomic force microscopy (AFM) – both considered as non‐invasive techniques – are applied to detect the biochemical and biophysical properties of trophoblast derived stem‐like cells incubated up to 10 days under conditions designed to induce differentiation. Significant biochemical and biophysical differences between control cells and differentiated cells were observed. Quantitative real time PCR was also applied to analyze gene expression. The relationship between cell differentiation and associated cellular biochemical and biomechanical changes were discussed.
2.
Luca Dalbosco Giulia Zanini Elvira D'Amato Francesco Tessarolo Sebastiana Boi Paolo Bauer Albrecht Haase Renzo Antolini 《Journal of biophotonics》2015,8(10):816-825
Photodamage, induced by femtosecond laser radiation, was studied in thick samples of human skin tissue (healthy skin and neoplastic lesions). Photobleaching, photoionization, and thermomechanical damage effects were characterized comparatively. The laser power dependence of the damage rates allowed to connect macroscopic effects to underlying molecular processes. Optical effects were correlated to histopathological changes. Tissue alterations were found only from thermomechanical cavitation and limited to superficial layers of the epidermis. From the depth‐dependencies of all damage thresholds a depth‐dependent power‐compensation scheme was defined allowing for damage‐free deep tissue optical biopsy.
3.
Rapid SERS monitoring of lipid‐peroxidation‐derived protein modifications in cells using photonic crystal
fiber sensor 下载免费PDF全文
Tianxun Gong Nan Zhang Kien Voon Kong Douglas Goh Cui Ying Jean‐Louis Auguste Perry Ping Shum Lei Wei Georges Humbert Ken‐Tye Yong Malini Olivo 《Journal of biophotonics》2016,9(1-2):32-37
We proposed a side channel photonic crystal fiber (SC‐PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation derived protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC‐PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference‐free alkyne Raman peak from these proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation derived protein modification in cells.
4.
Inside Back Cover: The conformation of bovine serum albumin adsorbed to the surface of single all‐dielectric nanoparticles following light‐induced heating (J. Biophotonics 7/2018) 下载免费PDF全文
Andrei A. Krasilin Katerina Volodina Arina A. Sukhova Mihail I. Petrov Dmitry A. Zuev Vyacheslav A. Dyachuk Valentin A. Milichko 《Journal of biophotonics》2018,11(7)
Germanium vs Silicon: All‐dielectric nanoparticles provides the heat resistance for proteins under light‐induced heating. Further details can be found in the article by Andrei A. Krasilin et al. ( e201700322 )
5.
Subcellular measurements of mechanical and chemical properties using dual Raman‐Brillouin microspectroscopy 下载免费PDF全文
Zhaokai Meng Sandra C. Bustamante Lopez Kenith E. Meissner Vladislav V. Yakovlev 《Journal of biophotonics》2016,9(3):201-207
Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background‐free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular‐ and subcellular‐level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA‐based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy.
6.
Dual‐polarization Raman spectral imaging to extract overlapping molecular fingerprints of living cells 下载免费PDF全文
Liang‐da Chiu Almar F. Palonpon Nicholas I. Smith Satoshi Kawata Mikiko Sodeoka Katsumasa Fujita 《Journal of biophotonics》2015,8(7):546-554
Raman spectral imaging is gaining more and more attention in biological studies because of its label‐free characteristic. However, the discrimination of overlapping chemical contrasts has been a major challenge. In this study, we introduce an optical method to simultaneously obtain two orthogonally polarized Raman images from a single scan of the sample. We demonstrate how this technique can improve the quality and quantity of the hyperspectral Raman dataset and how the technique is expected to further extend the horizons of Raman spectral imaging in biological studies by providing more detailed chemical information.
7.
Rapid and label‐free detection and assessment of bacteria by terahertz time‐domain spectroscopy 下载免费PDF全文
Shihan Yan Yueping Liu Shu Yu Mingkun Zhang Zhongbo Yang Xiaoyan Zhu Qing Huang Hong‐Liang Cui Weiling Fu 《Journal of biophotonics》2016,9(10):1050-1058
Here we demonstrated the potential and applicability of terahertz (THz) spectroscopy to detect four commonly found bacteria in the infectious diseases. Besides the different spectral characteristics between bacterial species, THz absorption differences for living bacteria, dead bacteria and bacterial powder of the same species were also investigated. Our results revealed that small differences in water contents between bacterial cells account for distinct discrepancies of the absorption coefficients, which can be used for bacterial species identification. Furthermore, living and dead bacteria showed different absorption coefficients as a result of their different hydration levels, suggesting that THz spectroscopy can be used to rapidly assess the living state of bacteria under test. Our results clearly demonstrated the ability of THz spectroscopy for time‐saving and label‐free detection of bacteria with minimal sample preparation, potentially to be utilized for point‐of‐care tests in the near future.
8.
Inside Cover: Fiber‐based fluorescence lifetime imaging of recellularization processes on vascular tissue constructs (J. Biophotonics 9/2018) 下载免费PDF全文
Alba Alfonso‐Garcia Jeny Shklover Benjamin E. Sherlock Alyssa Panitch Leigh G. Griffiths Laura Marcu 《Journal of biophotonics》2018,11(9)
Tissue autofluorescence provides fluorescence lifetime contrast between acellular tissue and that containing newly seeded cells. Fiber‐based fluorescence lifetime imaging (FLIm) can be used for tracking recellularization of engineered vascular grafts and potential matrix remodeling at large scale, without compromising sample integrity. FLIm cellular contrast was verified in a subset of samples seeded with eGFP‐labelled cells. Results suggests fiberbased FLIm is a suitable tool for monitoring recellularization of engineered tissue nondestructively. Further details can be found in the article by Alba Alfonso‐Garcia, Jeny Shklover, Benjamin E. Sherlock, et al. ( e201700391 ).
9.
Probe‐based confocal laser endomicroscopy (pCLE) – a new imaging technique for in situ localization of spermatozoa 下载免费PDF全文
Matthias Trottmann Herbert Stepp Ronald Sroka Michael Heide Bernhard Liedl Sven Reese Armin J. Becker Christian G. Stief Sabine Kölle 《Journal of biophotonics》2015,8(5):415-421
In azoospermic patients, spermatozoa are routinely obtained by testicular sperm extraction (TESE). However, success rates of this technique are moderate, because the site of excision of testicular tissue is determined arbitrarily. Therefore the aim of this study was to establish probe‐based laser endomicroscopy (pCLE) a noval biomedical imaging technique, which provides the opportunity of non‐invasive, real‐time visualisation of tissue at histological resolution. Using pCLE we clearly visualized longitudinal and horizontal views of the tubuli seminiferi contorti and localized vital spermatozoa. Obtained images and real‐time videos were subsequently compared with confocal laser scanning microscopy (CLSM) of spermatozoa and tissues, respectively.
10.
Label‐free,zeptomole cancer biomarker detection by surface‐enhanced fluorescence on nanoporous gold disk plasmonic nanoparticles 下载免费PDF全文
Greggy M. Santos Fusheng Zhao Jianbo Zeng Ming Li Wei‐Chuan Shih 《Journal of biophotonics》2015,8(10):855-863
We experimentally demonstrate a label‐free biosensor for the ERBB2 cancer gene DNA target based on the distance‐dependent detection of surface‐enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non‐porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.
11.
Label‐free and non‐invasive discrimination of HaCaT
and melanoma cells in a co‐culture model
by hyperspectral confocal reflectance microscopy 下载免费PDF全文
Francesca R. Bertani Elisabetta Botti Luisa Ferrari Valentina Mussi Antonio Costanzo Marco D'Alessandro Francesco Cilloco Stefano Selci 《Journal of biophotonics》2016,9(6):619-625
A novel hyperspectral confocal microscopy method to separate different cell populations in a co‐culture model is presented here. The described methodological and instrumental approach allows discrimination of different cell types using a non‐invasive, label free method with good accuracy with a single cell resolution. In particular, melanoma cells are discriminated from HaCaT cells by hyperspectral confocal imaging, principal component analysis and optical frequencies signing, as confirmed by fluorescence labelling cross check. The identification seems to be quite robust to be insensitive to the cellular shape within the studied samples, enabling to separate cells according to their cytotype down to a single cell sensitivity.
12.
Robert Koprowski 《Journal of biophotonics》2015,8(11-12):935-943
The paper presents problems and solutions related to hyperspectral image pre‐processing. New methods of preliminary image analysis are proposed. The paper shows problems occurring in Matlab when trying to analyse this type of images. Moreover, new methods are discussed which provide the source code in Matlab that can be used in practice without any licensing restrictions.
13.
Mechanisms of renal autoregulation generate oscillations in arterial blood flow at several characteristic frequencies. Full‐field laser speckle flowmetry provides a real‐time imaging of superficial blood microcirculation. The possibility to detect changes in oscillatory dynamics is an important issue in biomedical applications. In this paper we show how laser power density affects quality of the recorded signal and improves detectability of temporal changes in microvascular perfusion.
14.
A composite fibre optic catheter for monitoring
peristaltic transit of an intra‐luminal bead 下载免费PDF全文
John W. Arkwright Ian D. Underhill Kelsi N. Dodds Simon J. H. Brookes Marcello Costa Nick J. Spencer Phil. G. Dinning 《Journal of biophotonics》2016,9(3):305-310
A fibre optic motion sensor has been developed for monitoring the proximity and direction of motion of a ferrous bead travelling axial to the sensor. By integrating an array of these sensors into our previously developed fibre optic manometry catheters we demonstrate simultaneous detection of peristaltic muscular activity and the associated motion of ferrous beads through a colonic lumen. This allows the motion of solid content to be temporally and spatially related to pressure variations generated by peristaltic contractions without resorting to videoflouroscopy to track the motion of a radio opaque bolus. The composite catheter has been tested in an in‐vitro animal preparation consisting of excised sections of rabbit colon.
15.
Earu Banoth Vamshi Krishna Kasula Veerendra Kalyan Jagannadh Sai Siva Gorthi 《Journal of biophotonics》2016,9(6):610-618
In this work, an optofluidic flow analyzer, which can be used to perform malaria diagnosis at the point‐of‐care is demonstrated. The presented technique is based on quantitative optical absorption measurements carried out on a single cell level for a given population of Human Red Blood Cells (RBCs). By measuring the optical absorption of each RBC, the decrease in the Hemoglobin (Hb) concentration in the cytoplasm of the cell due to the invasion of malarial parasite is detected. Cells are assessed on a single cell basis, as they pass through a microfluidic channel. The proposed technique has been implemented with inexpensive off‐the‐shelf components like laser diode, photo‐detector and a micro‐controller. The ability of the optofluidic flow analyzer to asses about 308,049 cells within 3 minutes has been demonstrated. The presented technique is capable of detecting very low parasitemia levels with high sensitivity.
16.
Raman spectroscopy of serum: A study on ‘pre’ and ‘post’ breast adenocarcinoma resection in rat models 下载免费PDF全文
Tanmoy Bhattacharjee Aarif Khan Piyush Kumar Arvind Ingle Girish Maru C. Murali Krishna 《Journal of biophotonics》2015,8(7):575-583
Risk of recurrence is a major problem in breast cancer management. Currently available prognostic markers have several disadvantages including low sensitivity and specificity, highlighting the need for new prognostic techniques. One of the candidate techniques is serum‐based Raman spectroscopy (RS). In this study, feasibility of using RS to distinguish ‘pre’ from ‘post’ breast tumor resection serum in rats was explored. Spectral analysis suggests change in proteins and amino acid profiles in ‘post’ compared to ‘pre‐surgical’ group. Principal‐Component‐Linear‐Discriminant‐Analysis shows 87% and 91% classification efficiency for ‘pre’ and ‘post‐surgical’ groups respectively. Thus, the study further supports efficacy of RS for theranostic applications.
17.
Front Cover: Dual‐wavelength hybrid optoacoustic‐ultrasound biomicroscopy for functional imaging of large‐scale cerebral vascular networks (J. Biophotonics 9/2018) 下载免费PDF全文
Johannes Rebling Héctor Estrada Sven Gottschalk Gali Sela Michael Zwack Georg Wissmeyer Vasilis Ntziachristos Daniel Razansky 《Journal of biophotonics》2018,11(9)
We present a hybrid dual‐wavelength optoacoustic and ultrasound bio‐microscope capable of rapid transcranial visualization of morphology and oxygenation status of large‐scale cerebral vascular networks. Imaging of entire cortical vasculature in mice is achieved with single capillary resolution and complemented by simultaneously acquired pulse‐echo ultrasound microscopy scans of the mouse skull. The new approach holds potential to facilitate studies into neurological and vascular abnormalities of the brain. Further details can be found in the article by Johannes Rebling, Héctor Estrada, Sven Gottschalk, et al. ( e201800057 ).
18.
Synergistic effects of atmospheric pressure plasma‐emitted components on DNA oligomers: a Raman spectroscopic study 下载免费PDF全文
Eugen Edengeiser Jan‐Wilm Lackmann Erik Bründermann Simon Schneider Jan Benedikt Julia E. Bandow Martina Havenith 《Journal of biophotonics》2015,8(11-12):918-924
Cold atmospheric‐pressure plasmas have become of increasing importance in sterilization processes especially with the growing prevalence of multi‐resistant bacteria. Albeit the potential for technological application is obvious, much less is known about the molecular mechanisms underlying bacterial inactivation. X‐jet technology separates plasma‐generated reactive particles and photons, thus allowing the investigation of their individual and joint effects on DNA. Raman spectroscopy shows that particles and photons cause different modifications in DNA single and double strands. The treatment with the combination of particles and photons does not only result in cumulative, but in synergistic effects. Profilometry confirms that etching is a minor contributor to the observed DNA damage in vitro.
19.
Quantitative and rapid estimations of human sub‐surface skin mass using ultra‐high‐resolution spectral domain optical coherence tomography 下载免费PDF全文
Non‐invasive and quantitative estimations for the delineation of sub‐surface tumor margins could greatly aid in the early detection and monitoring of the morphological appearances of tumor growth, ensure complete tumor excision without the unnecessary sacrifice of healthy tissue, and facilitate post‐operative follow‐up for recurrence. In this study, a high‐speed, non‐invasive, and ultra‐high‐resolution spectral domain optical coherence tomography (UHR‐SDOCT) imaging platform was developed for the quantitative measurement of human sub‐surface skin mass. With a proposed robust, semi‐automatic analysis, the system can rapidly quantify lesion area and shape regularity by an en‐face‐oriented algorithm. Various sizes of nylon sutures embedded in pork skin were used first as a phantom to verify the accuracy of our algorithm, and then in vivo, feasibility was proven using benign human angiomas and pigmented nevi. Clinically, this is the first step towards an automated skin lesion measurement system.
20.
An infrared sensor analysing label‐free the secondary structure of the Abeta peptide in presence of complex fluids 下载免费PDF全文
Andreas Nabers Julian Ollesch Jonas Schartner Carsten Kötting Just Genius Ute Haußmann Hans Klafki Jens Wiltfang Klaus Gerwert 《Journal of biophotonics》2016,9(3):224-234
The secondary structure change of the Abeta peptide to beta‐sheet was proposed as an early event in Alzheimer's disease. The transition may be used for diagnostics of this disease in an early state. We present an Attenuated Total Reflection (ATR) sensor modified with a specific antibody to extract minute amounts of Abeta peptide out of a complex fluid. Thereby, the Abeta peptide secondary structure was determined in its physiological aqueous environment by FTIR‐difference‐spectroscopy. The presented results open the door for label‐free Alzheimer diagnostics in cerebrospinal fluid or blood. It can be extended to further neurodegenerative diseases.