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1.
Ian K. Ross 《American journal of botany》1961,48(3):244-248
Ross, Ian K. (Yale U., New Haven, Conn.) Further studies on meiosis in the Myxomycetes. Amer. Jour. Bot. 48(3): 244–248. Illus. 1961.—Photomicrographs of meiotic figures in 5 species of Myxomycetes are presented as further evidence of the time and location of meiosis in these organisms. Three members of the Physarales studied differed from species studied previously in that the cleavage of the protoplasm began during the second meiotic division and not after the divisions had been completed. Chromosome numbers were found to be in accord with previous observations and ranged from n = 25 to 90 depending on the species. 相似文献
2.
In this study, the chromosome constitution of mouse eggs exposed in vivo to a dilute solution of ethanol during specific stages of the first and second meiotic divisions was determined at the first cleavage mitosis. Exposure to ethanol prior to the completion of the second meiotic division induced an incidence (7-10%) of aneuploidy involving only one chromosome in 98% of malsegregation events. This investigation provides indirect evidence that ethanol may induce aneuploidy by disrupting the functioning of the meiotic spindle. Karyological analyses of chromosome spreads prepared at the first cleavage metaphase suggest that only a small proportion of the total chromosome complement may be induced to undergo malsegregation. 相似文献
3.
Emphasis is placed on three aspects of meiosis in the moss Amblystegium riparium (Hedw.) BSG: 1***) nature of the sporogenous layer; 2) prophasic microtubules and polarity; and 3) cleavage pattern. Spore tetrads develop while still encased by archesporial cell walls. The cellular nature of the sporogenous layer differs from the more usual occurrence of free sporocytes released into a common spore sac. Two important events mark the establishment of sporocyte polarity during meiotic prophase: 1) migration of the four plastids to the distal tetrad poles (telophase II poles); and 2) ingrowth of the sporocyte wall in eventual cleavage planes between the tetrad poles. An extensive, plastid-based microtubule system is associated with organelle migration during the establishment of sporocyte polarity in meiotic prophase. Disruption of the nuclear envelope in prometaphase I occurs at sites opposite the four plastids where microtubules extend from plastid envelope to nuclear envelope. Formation of a cell plate following the first meiotic division results in a dyad, whereas in many mosses meiosis is completed in the undivided sporocyte and is followed by simultaneous cleavage into a spore tetrad. Spore cleavage is accomplished by vesicular coalescence resulting in septa that coincide with the prophasic wall ingrowths. 相似文献
4.
Summary Of 61 families of children with trisomy 21, polymorphism of chromosome 21 elucidating the origin of the extra chromosome was found in 42. Nondisjunction was of paternal origin in 8 cases (19.04%) and the anomaly occurred with equal frequency during the first and second meiotic divisions. Maternal nondisjunction was demonstrated in 34 cases (80.95%), in which nondisjunction occurred by far the most often during the first meiotic division (29 cases).These results are in agreement with data from the literature, and suggest the existence of at least two different causes for chromosomal nondisjunction, the first being the same in both sexes and occurring in both meiotic divisions and the second specifically limited to the first meiotic division in the mother.Attachée de Recherche au CNRSAttachée de Recherche à l'INSERM 相似文献
5.
NORIYUKI SATOH 《Development, growth & differentiation》1977,19(2):111-117
The cleavage pattern in the egg of Xenopus laevis has been investigated with the aid of time-lapse cinematography. From the 5th cleavage onward, divisions of the surface blastomeres are not synchronous but metachronous. A few blastomeres in a very restricted region which is situated in most cases in the dorsal side of the animal hemisphere, slightly distant from the median line and near the equatorial junction of the animal and vegetal hemispheres, divide before the other blastomeres, and a wave-like propagation of the divisions travels along the surface from that region toward the animal and vegetal poles. The wave-like propagation ends in the vegetal pole region. In the animal hemisphere, this pattern of cleavage is continued until the 13th cleavage and thereafter the divisions of surface blastomeres become asynchronous. In the vegetal pole region, however, the 14th metachronous division of blastomeres is clearly observed in the film. Gastrulation begins after 14 cleavages. 相似文献
6.
Dieter Ammermann 《Chromosoma》1967,23(2):203-213
Hypsibius dujardini Doy. (Articulata, Tardigrada) shows obligatory parthenogenesis under given cultivating conditions. Males were never found. The first meiotic division reduces the number of chromosomes; the (2n=10) chromosomes are divided between a small polar body and the egg nucleus. Prior to the second division the dyads divide, thus restoring the diploid number. A diploid polar body is formed subsequent to the second division. After the egg nucleus has moved toward the center of the egg, the cleavage divisions begin. — During meiosis II and the first cleavage divisions the chromosomes can develop into “large chromosomes” which presumably consist mostly of RNA. No “large chromosomes” are found after the seventh cleavage division. Sometimes a plate of coloured material (“elimination chromatin”) can be observed between the anaphase daughter plates of the first cleavage divisions. In this case the chromosomes are always small. 相似文献
7.
Lois A. Pfiester P. Timpano J. J. Skvarla J. R. Holt 《American journal of botany》1984,71(8):1121-1127
In Peridinium inconspicuum Lemmermann, sexual reproduction occurs in both nitrogen-enriched and nitrogen-deficient media. In this homothallic strain, protoplasmic fusion begins between two thecate gametes; but zygote formation is completed in a space outside the fusing pair. This diploid cell can form a plated theca which is shed as the cell enlarges. This spherical zygote then forms a new non-plated theca. The process of ecdysis and the formation of a new non-plated theca is repeated several times. During this process the zygote gradually elongates and by cytoplasmic infurrowing becomes peanut-shaped. Eventually two cells are formed. The first and second meiotic divisions are greatly separated in time. The first meiotic division occurs in the spherical non-thecate zygote. The second meiotic division can occur in the peanut-shaped zygote before it completes cytokinesis. This meiotic division may not be synchronous, occasionally resulting in a trinucleate stage. Eventually four flagellated, haploid products are produced. 相似文献
8.
Dr. René Camenzind 《Chromosoma》1971,35(4):393-402
In paedogenetically developing female eggs of the gall midgeMycophila speyeri only one equational meiotic division occurs. The primary cleavage nucleus contains 29 chromosomes. In the fourth cleavage
division 23 chromosomes are eliminated from the future somatic nuclei while the primordial germ-line nucleus keeps the high
chromosome number.—The paedogenetic development of male eggs begins with two meiotic divisions. The egg nucleus with 14 or
15 chromosomes fuses with two, sometimes only one, somatic nuclei (2n=6) of maternal origin (regulation). Thus the primary
cleavage nucleus contains 26 or 27 chromosomes, sometimes only 20 or 21. Elimination in cleavage divisions V and VI leeds
to somatic nuclei with 3 chromosomes while the primordial germ-line nucleus keeps the high chromosome number.—Differences
between male and female eggs and the evolution of regulation in gall midges are discussed. 相似文献
9.
During mammalian oocyte maturation, two consecutive meiotic divisions are required to form a haploid gamete. For each meiotic division, oocytes must transfer from metaphase to anaphase, but maturation promoting factor (cyclin-dependent kinase 1/cyclin B1) activity would keep the oocytes at metaphase. Therefore, inactivation of maturation promoting factor is needed to finish the transition and complete both these divisions; this is provided through anaphase-promoting complex/cyclosome-dependent degradation of cyclin B1. The objective of this study was to examine meiotic divisions in bovine oocytes after expression of a full length cyclin B1 and a nondegradable N-terminal 87 amino acid deletion, coupled with the fluorochrome Venus, by microinjecting their complementary RNA (cRNA). Overexpression of full-length cyclin B1-Venus inhibited homologue disjunction and first polar body formation in maturing oocytes (control 70% vs. overexpression 16%; P < 0.05). However at the same levels of expression, it did not block second meiotic metaphase and cleavage of eggs after parthenogenetic activation (control: 82% pronuclei and 79% cleaved; overexpression: 91% pronuclei and 89% cleaved). The full length cyclin B1 and a nondegradable N-terminal 87 amino acid deletion caused metaphase arrest in both meiotic divisions, whereas degradation of securin was unaffected. Roscovitine, a potent cyclin-dependent kinase 1 (CDK1) inhibitor, overcame this metaphase arrest in maturing oocytes at 140 μM, but higher doses (200 μM) were needed to overcome arrest in eggs. In conclusion, because metaphase I (MI) blocked by nondegradable cyclin B1 was distinct from metaphase II (MII) in their different sensitivities to trigger CDK1 inactivation, we concluded that mechanisms of MI arrest differed from MII arrest. 相似文献
10.
The oocytes of B6.Y(TIR) sex-reversed female mice can be fertilized but the resultant embryos die at early cleavage stages. In the present study, we examined chromosome segregation at meiotic divisions in the oocytes of XY female mice, compared to those of XX littermates. The timing and frequency of oocyte maturation in culture were comparable between the oocytes from both types of females. At the first meiotic division, the X- and Y-chromosomes segregated independently and were retained in oocytes at equal frequencies. However, more oocytes retained the correct number of chromosomes than anticipated from random segregation. The oocytes that had reached MII-stage were activated by fertilization or incubation with SrCl(2). As expected, the majority of oocytes from XX females completed the second meiotic division and reached the 2-cell stage in 24 h. By contrast, more than half of oocytes from XY females initially remained at the MII-stage while the rest precociously entered interphase after SrCl(2) activation; very few oocytes were seen at the second anaphase or telophase and they often showed impairment of sister-chromatid separation. Eventually the majority of oocytes entered interphase and formed pronuclei, but very few reached the 2-cell stage. Similar results were obtained after fertilization. We conclude that the XY chromosomal composition in oocyte leads to impairment in the progression of the second meiotic division. 相似文献
11.
Katis VL Matos J Mori S Shirahige K Zachariae W Nasmyth K 《Current biology : CB》2004,14(24):2183-2196
BACKGROUND: Cells undergoing meiosis perform two consecutive divisions after a single round of DNA replication. During the first meiotic division, homologous chromosomes segregate to opposite poles. This is achieved by (1) the pairing of maternal and paternal chromosomes via recombination producing chiasmata, (2) coorientation of homologous chromosomes such that sister chromatids attach to the same spindle pole, and (3) resolution of chiasmata by proteolytic cleavage by separase of the meiotic-specific cohesin Rec8 along chromosome arms. Crucially, cohesin at centromeres is retained to allow sister centromeres to biorient at the second division. Little is known about how these meiosis I-specific events are regulated. RESULTS: Here, we show that Spo13, a centromere-associated protein produced exclusively during meiosis I, is required to prevent sister kinetochore biorientation by facilitating the recruitment of the monopolin complex to kinetochores. Spo13 is also required for the reaccumulation of securin, the persistence of centromeric cohesin during meiosis II, and the maintenance of a metaphase I arrest induced by downregulation of the APC/C activator CDC20. CONCLUSION: Spo13 is a key regulator of several meiosis I events. The presence of Spo13 at centromere-surrounding regions is consistent with the notion that it plays a direct role in both monopolin recruitment to centromeres during meiosis I and maintenance of centromeric cohesion between the meiotic divisions. Spo13 may also limit separase activity after the first division by ensuring securin reaccumulation and, in doing so, preventing precocious removal from chromatin of centromeric cohesin. 相似文献
12.
The morphology of mitochondrial nucleoids (mt-nucleoids), mitochondria, and nuclei was investigated during meiosis and sporulation of the diploid cells of the ascosporogenic yeast Saccharomycodes ludwigii. The mt-nucleoids appeared as discrete dots uniformly distributed in stationary-phase cells as revealed by 4',6-diamidino-2-phenylindole (DAPI) staining. Throughout first and second meiotic divisions, the mt-nucleoids moved to be located close to the dividing nuclei with the appearance of dots. On the other hand, mitochondria, which had tubular or fragmented forms in stationary-phase cells, increasingly fused with each other to form elongated mitochondria during meiotic prophase as revealed by 3,3' -dihexyloxacarbocyanine iodide [DiOC(6)(3)] staining. Mitochondria assembled to be located close to dividing nuclei during first and second meiotic divisions, and were finally incorporated into spores. During the first meiotic division, nuclear division occurred in any direction parallel, diagonally, or perpendicular to the longitudinal axis of the cell. In contrast, the second meiotic division was exclusively parallel to the longitudinal axis of the cell. The behavior of dividing nuclei explains the formation of a pair of spores with opposite mating types at both ends of cells. In the course of this study, it was also found that ledges between two spores were specifically stained with DiOC(6)(3). 相似文献
13.
Using the starfish oocyte and zygote, we investigated the abilities of the centrosome at maturation and cleavage divisions to form the aster and induce cytokinesis, in order to determine differences between these divisions. The transplanted centrosome originated from both maturation and cleavage, induced an additional furrow in cleavage in the recipient cells, but did not induce abnormal polar body formation at maturation. Although it organized an additional aster in the recipient cell in both divisions, a difference in size among asters formed was recognized. Therefore, mitotic asters were stabilized with hexylene glycol in order to measure their radius and clarify this difference. The mean radius (14.4 μm) of the first meiotic aster was significantly smaller than that (20.4 μm) of the aster at the first cleavage. The transplanted cleavage centrosome formed as small an aster as the recipient's own at maturation divisions. When zygotes were briefly treated with colcemid so that the zygotes could not perform cytokinesis but did perform karyokinesis, the size of aster became the same as that in meiosis. These results prove that although any centrosome functions as a microtubule organizing center independent of its origin, the size of the resultant aster decides whether or not cytokinesis would be induced. 相似文献
14.
1. Meiosis in the Oomycetes is gametangial. 2. The life-cycle of the Oomycetes is therefore haplobiontic, type B. 3. The gametangia are multinucleate prior to septation. Vegetative nuclear divisions may occur in the hyphae subtending the gametangia, but there is no evidence for such divisions occurring in the gametangial primordia nor is there any indication that nuclei may move out of the primordium against any cytoplasmic flow. 4. Some abortion of supernumerary nuclei probably occurs after the gametangium is cut off from the vegetative thallus by the septum. Meiosis then takes place. 5. The spindle of the first metaphase is almost certainly within a persistent nuclear membrane, but there remains some doubt as to whether this membrane persists to the second telophase in all Oomycetes. 6. In the higher Peronosporales, and possibly the Rhipidiaceae, meiosis is accompanied or preceded by zonation into the periplasm and ooplasm. Spindle orientation and the timing of zonation movements probably account for the differences in the number of presumptive oosphere nuclei recorded between many Peronosporales. In some Albuginaceae, at least, it is possible that only one nucleus completes the meiotic division, but this needs confirmation. 7. A smaller number of nuclei enter the male gametangium and undergo a more or less simultaneous meiosis. 8. Some variation in the pattern and degree of synchrony of meiotic division within and between gametangia occurs in different species. 9. Nuclear abortions may precede, accompany or follow meiosis, but only in a few instances (Pythium debaryanum, P. deliense, Phytophthora himalayensis, Aphanomyces laevis) does the male gametangium finally contain only a single gamete nucleus. 10. Cytoplasmic cleavage, involving the tonoplast and central vacuole of the oogonium, occurs after meiosis in the Saprolegniales, thus offering an alternative mechanism to zonation movements for the production of uninucleate oospheres. The presence (Edson, 1915; Patterson, 1927b; Murphy, 1918) or absence (Trow, 1901; Saskena, 1936) or an homologous central vacuole in the Pythiaceae is disputed. 11. Karyogamy must follow antheridial penetration in those species which are not agamospermous, but the degree of facultative agamospermy is unknown. The timing of karyogamy, as opposed to somatogamy, is apparently variable between and within species (Wager, 1899; Arens, 1929, Moreau & Moreau, 1935; McDonough, 1937; Flanagan, 1970; Win-Tin, 1972). There are a few indications that karyogamy may be precocious and other evidence that it may be considerably delayed, even after the oospore has achieved morphological maturity, and exceptionally until germination. 12. It would appear that the majority of the oospores of most Oomycetes eventually contain only one fusion or diploid nucleus, but there are exceptions (Albugo bliti, A. platensis, A. portulacae and Aplanopsis terrestris respectively) and without further study it would be unwise to assume that this is necessarily true even for closely related species. 13. Mitosis immediately following karyogamy is reported as occurring in some species of Albugo, but in most Oomycetes it is delayed until the period immediately preceding any cytoplasmic or morphological change at the start of germination. 14. The nuclear divisions of the germinating oospore are mitotic, but they may differ in the detailed morphology of the spindle apparatus or the degree of condensation of the chromosomes. 15. Interpretations of the cytology of the small nuclei of the Oomycetes have been profoundly influenced by the prevailing climates of scientific opinion. In particular, the development of studies of meiosis and the science of genetics on the one hand, and the appreciation of the polyphyletic origin of the fungi, especially the algal origins of the Oomycetes on the other hand, have necessitated a re-evaluation of much of the older literature. 相似文献
15.
Lorin W. Roberts 《American journal of botany》1960,47(2):110-114
Roberts , Lorin W. (U. Idaho, Moscow.) Protein-bound sulfhydryl groups in Coleus wound meristems. Amer. Jour. Bot. 47(2): 110—114. Illus. 1960.–A hisiochemical examination was conducted of the development of the stem wound-meristem of Coleus blumei Benth. with 2,2‘-dihydroxy-6,6‘-dinaphthyl disulfide (DDD) and 1-(4-chloromercuriphenylazo)-naphthol-2 (Mercury Orange) to test for protein-bound sulfhydryl groups. Specificity for the histochemical reactions was indicated by complete blocking of staining in sections pretreated with 0.001 M iodine in npropanol and 0.1 M aqueous solutions of iodoaceiamide. Both histochemical methods yielded comparable results in protein-SH localization (zone of cell division and differentiating xylem elemments) and staining intensity. The zone of cell division did not exhibit staining at the time of initial cell division (3 days). However, the cytoplasm of the dividing cells of the wound-meristem demonstrated protein-SH 4—5 days following wounding. A high concentration of protein-SH was observed in the cytoplasm of the dividing cells 7 days after wounding. Redifferentiating parenchyma cells, destined to become wound-xylem elements, first exhibited wall staining at the time of protoplast contraction. The subsequent walled xylem stage exhibited strong staining in the reticulated striations of the secondary walls. 相似文献
16.
Zernicka-Goetz M 《Seminars in cell & developmental biology》2004,15(5):563-572
A growing body of evidence indicates that although the early mouse embryo retains flexibility in responding to perturbations, its patterning is initiated at the earliest developmental stages. There are a few spatial cues that are able to influence the pattern of cleavage divisions: one of these lies in the vicinity of the previous meiotic division, the second is associated with the sperm entry and, related to this, the third is the cell shape. Furthermore, the first cleavage separates the zygote into two cells that tend to follow distinguishable fates: one contributes mainly to the embryonic part of the blastocyst, and the other to the abembryonic. The cumulative effect of the early asymmetries generated through cleavage might lead to asymmetric interactions between the first lineages of cells. This could influence development of patterning after implantation. These early polarity cues serve to bias patterning and not as definitive determinants. 相似文献
17.
This is the second of two papers which together are the first comprehensive ultrastructural report of meiosis in a red alga. Many details of the meiotic process in Dasya baillouviana (Gmelin) Montagne are the same as those reported previously for mitotic cells in ceramialian red algae, but several characteristics seem unique to meiotic cells. The nucleus and nucleolus of meiotic cells are larger than those of mitotic cells and large accumulations of smooth ER are often found at the division poles during meiosis 1. The function of the ER accumulations is unknown. Importantly, both interkinesis and a simultaneous division of two separate nuclei during meiosis II was demonstrated. These new observations fail to support earlier speculation on higher red algae for a “uninuclear” meiosis (both nuclear divisions within the same nuclear envelope). However, following meiosis II the four nuclei migrate centripetally and possibly fuse in the center of the tetrasporangium. This post-division nuclear maneuvering is not understood, but our interpretation accounts for the earlier and erroneous impression of “uninuclear” meiosis. Perhaps the most important aspect of meiosis observed in Dasya is its basic adherence to the pattern commonly seen in higher plants and animals. This conservatism of the meiotic process lends further skepticism to the belief that red algae are extremely “primitive” organisms, although they undoubtedly represent a very “ancient” group of eukaryotic plants. 相似文献
18.
Rabitsch KP Gregan J Schleiffer A Javerzat JP Eisenhaber F Nasmyth K 《Current biology : CB》2004,14(4):287-301
BACKGROUND: Meiosis produces haploid gametes from diploid progenitor cells. This reduction is achieved by two successive nuclear divisions after one round of DNA replication. Correct chromosome segregation during the first division depends on sister kinetochores being oriented toward the same spindle pole while homologous kinetochores must face opposite poles. Segregation during the second division depends on retention of sister chromatid cohesion between centromeres until the onset of anaphase II, which in Drosophila melanogaster depends on a protein called Mei-S332 that binds to centromeres. RESULTS: We report the identification of two homologs of Mei-S332 in fission yeast using a knockout screen. Together with their fly ortholog they define a protein family conserved from fungi to mammals. The two identified genes, sgo1 and sgo2, are required for retention of sister centromere cohesion between meiotic divisions and kinetochore orientation during meiosis I, respectively. The amount of meiotic cohesin's Rec8 subunit retained at centromeres after meiosis I is reduced in Deltasgo1, but not in Deltasgo2, cells, and Sgo1 appears to regulate cleavage of Rec8 by separase. Both Sgo1 and Sgo2 proteins localize to centromere regions. The abundance of Sgo1 protein normally declines after the first meiotic division, but extending its expression by altering its 3'UTR sequences does not greatly affect meiosis II. Its mere presence within the cell might therefore be insufficient to protect centromeric cohesion. CONCLUSIONS: A conserved protein family based on Mei-S332 has been identified. The two fission yeast homologs are implicated in meiosis I kinetochore orientation and retention of centromeric sister chromatid cohesion until meiosis II. 相似文献
19.
Cyclic assembly-disassembly of cortical microtubules during maturation and early development of starfish oocytes 总被引:7,自引:0,他引:7
An extensive array of cortical microtubules in oocytes of the starfish Pisaster ochraceus undergoes multiple cycles of disappearance and reappearance during maturation and early development. These events were studied in isolated fragments of the oocyte cortex stained with antitubulin antibodies for indirect immunofluorescence. The meshwork of long microtubules is present in the cortex (a) of immature oocytes, i.e., before treatment with the maturation-inducing hormone 1-methyladenine, (b) for 10-20 min after treatment with 1-methyladenine, (c) after formation of the second polar body (in reduced numbers in unfertilized oocytes), and (d) in the intermitotic period between first and second cleavage divisions. The array of cortical microtubules is absent in oocytes (a) undergoing germinal vesicle breakdown, (b) during the two meiotic divisions (polar body divisions), and (c) during mitosis of the first and, perhaps, subsequent cleavage divisions. The cycle of assembly-disassembly of cortical microtubules is synchronized to the cycle of nuclear envelope breakdown and reformation and to the mitotic cycle; specifically, cortical microtubules are present when a nucleus is intact (germinal vesicle, female pronucleus, zygote nucleus, blastomere nucleus) and are absent whenever a meiotic or mitotic spindle is present. These findings are discussed in terms of microtubule organizing centers in eggs, possible triggers for microtubule assembly and disassembly, the eccentric location of the germinal vesicle, and the regulation of oocyte maturation and cell division. 相似文献
20.
Gametogenesis in Atractomorpha porcata Hoffman was initiated b the synchronous mitotic division of nuclei within a multinucleate gametangium. Uninucleate gametes were subsequently produced following two series of cytokinetic divisions. The first series involved the formation of phycoplast microtubules (phycoplastic cytokinesis), whereas the second series did not (nonphycoplastic cytokinesis). Centrioles were connected by a rudimentary striated distal fiber by the time they migrated to the planes of division preceding the first series of cytokinetic division. These first divisions produced binucleate gametocytes. A well-developed flagellar apparatus lay near the cell surface in close proximity to each nucleus of the gametocyte prior to the second series of cytokinetic divisions that produced the uninucleate gametes. As seen in apical view, the paired basal bodies were directly opposed, with no lateral displacement of their longitudinal axes. In lateral view, the paired basal bodies diverged from one another at an angle of 130–180° (female) or 170–180° (male) and were connected by an arched, distal striated fiber about 670–750 nm long and 600 nm at its widest part. Four electron-opaque, pyramid-shaped lateral bodies flanked the basal bodies in close contact with their undersurfaces. The flagellar roots demonstrated a cruciate arrangement, with s = 6–9 over 1 (female gametes) or 7–10 over 1 (male gametes) microtubules and d= 2 microtubules. In male gametes, one of the multistranded roots was located close to the eyespot, and a second system of cytoskeletal microtubules was detected internally. Based on gamete ultrastructure, Atractomorpha porcata appears to be the most undifferentiated member of the genus. 相似文献