Organotype culture of glandular tissue of the testis of Gabius niger L. Association with hypophysis]

The endocrine gland of the testis of Gobius niger L. was cultured separately for three or six days by dissecting out from the seminal mass. During the sexual resting periode, the explantation don't induce histological changes; however, during the phase of high sexual activity, the explantation involves marked asthenic signs. This phenomenon is in all cases reversed by the association with pituitaries, even after a preliminary isolated culture of three days. The association with pituitaries increases also the functional activity of the explants compared with the controls tested by measuring the total amount of testosterone contained in pooled culture media, or the global metabolism of 14C sodium acetate.