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1.
E.A. Kozlov T.L. Levitina N.M. Sidorova Yu.L. Radavski S.B. Serebryani 《Journal of invertebrate pathology》1975,25(1):103-107
Amino and carboxyl terminal groups, amino acid composition, and peptide maps of polyhedral proteins of the nuclear polyhedrosis viruses (NPV) of Bombyx mori and Galleria mellonella were investigated. It is shown that both the proteins have a tyrosine residue as their carboxyl terminal group and no amino terminal group. Amino acid compositions of the proteins are similar. The proteins are found to have 242 residues. From the amino acid composition, a molecular weight of 28,000 was calculated. The tryptic peptide maps of both the proteins differed only in a few peptides.It is inferred that the polyhedral proteins of B. mori and G. mellonella NPV have a closely similar primary structure. 相似文献
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Polyhedral inclusion body protein (PIBP), harvested from the nuclear polyhedrosis virus (NPV) infecting the gypsy moth, Porthetria dispar, illicits the hemagglutination of chicken erythrocytes. Antisera to PIBP, polyhedral inclusion bodies (PIB), and virions (RODS) from the NPV's infecting P. dispar and the European Pine Sawfly, Neodiprion sertifer, inhibits hemagglutination when utilized to neutralize the PIBP from P. dispar. The crossreactivity of antisera to viral components from N. sertifer, a hymenopteran insect, with viral antigens from P. dispar, a lepidopteran insect, demonstrates a serological relationship exists between two viruses which have widely separated host ranges. 相似文献
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Immunodiffusion and tube precipitation tests, polyacrylamide gel electrophoresis of virus polypeptides, and cross-transmission experiments suggest that two nuclear polyhedrosis viruses, one from Lymantria monacha and one from L. dispar, are partially related to each other, but not identical. The virus particle proteins seem to be more specific than the polyhedron proteins. 相似文献
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Robert J. Cibulsky James D. Harper Robert T. Gudauskas 《Journal of invertebrate pathology》1977,29(2):182-191
Polyhedral protein preparations from five nuclear polyhedrosis viruses isolated from four closely related host insects of the noctuid subfamily Plusiinae were characterized by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), high voltage paper electrophoresis, and amino acid analysis. The viruses were Autographa california multiple-embedded virion type (MEV), Pseudoplusia includens singly embedded virion type (SEV), Rachiplusia ou MEV, Trichoplusia ni MEV, and T. ni SEV. Each was produced in its own host; A. californica MEV was also produced in T. ni larvae to determine possible host influence on polyhedral protein chemistry. Each test revealed minor, reproducible differences among most isolates. In SDS-PAGE, the major protein component ranged from 26,700 to 28,300 MW among the isolates. Differences were confined to minor protein bands or to band intensity. Peptide maps showed differences among most isolates in numbers of acidic and basic peptide spots, but all had an identical number of neutral spots. Migration patterns also differed among most isolates. The amino acid compositions of the six polyhedral inclusions were very similar, with aspartic and glutamic acids being the predominant residues. The greatest differences were found between the MEV and SEV groups, with lesser differences within each group. In all analyses, A. californica MEV produced in A. californica was indistinguishable from virus produced in T. ni. 相似文献
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The serological relationships of five nuclear polyhedrosis viruses (NPV) were investigated using the immunodiffusion technique with intragel absorption. Reciprocal tests demonstrated that virion fractions from Autographa californica multiple embedded virus (MEV), Heliothis armigera MEV, and H. zea single embedded virus (SEV) are not related to each other or to virions from Trichoplusia ni SEV and Pseudoplusia includens SEV. Virion fractions of T. ni and P. includens NPV were shown to be closely related, sharing several antigens. Matrix fractions possessed a common group antigen and one or two antigens specific for the individual NPV with the exception that T. ni and P. includens NPV shared one of these antigens. The specific antigens of the matrix fraction were also shared with the homologous virion fraction. 相似文献
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The virulence of 5 nuclear polyhedrosis viruses infectious for larvae of beet armyworm,Spodoptera exigua, was studied and their potential as biological control agents of this accidentally introduced pest in Dutch greenhouse crops
is discussed. Three of the virus isolates were collected from deceased beet armyworm larvae found in Dutch greenhouses. Based
on restriction endonuclease patterns of their DNA they appeared to be closely related toMamestra brassicae nuclear polyhedrosis virus (MbMNPV) and therefore were named MbMNPV-NL80, MbMNPV-NL82 and MbMNPV-NL83. These isolates were
not related toAutographa californica MNPV (AcMNPV) or toSpodoptera exigua MNPV (SeMNPV), both originating from the USA.
Comparison of the oiological activity of these 5 isolates showed that the SeMNPV was more virulent against beet armyworm than
the other isolates. There was no significant difference in virulence between MbMNPV-NL80, NL82, NL83 and AcMNPV forS. exigua. The LD-50 values of the 5 isolates for 2nd instar larvae were 3, 26, 14, 17 and 18 polyhedra, respectively. Despite compensating
qualities of the other MNPVs, such as a broader host range and potential production in alternate hosts or cell-lines, SeMNPV
is considered to be the most suitable candidate as biological control agent of beet armyworm.
相似文献
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G I Fedorova R Ia Podcherniaeva A M Amchenkova N I Nikitina V K Blinova 《T?Sitologii?a i genetika》1974,8(5):396-399
When the Drosophila cells were infected with the mixo- and arboviruses, in case of influenza A/WSN virus a rise in the titre and slight cytopathogenic effect with the subsequent decrease in the titre was observed. Since the decrease in the virus titer was not observed when actinomycin D was added, it was supposed that interferonlike inhibitor may be produced by the infected cells. Vacuolization and increase in the size of the infected cells were caused by all the nuclear polyhedrosis viruses tested. The number of the infected cells depended on the virus type and multiplicity of the infection. 相似文献
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Robert J. Cibulsky James D. Harper Robert T. Gudauskas 《Journal of invertebrate pathology》1977,30(3):303-313
The virions of six isolates of five nuclear polyhedrosis viruses (NPV) infecting plusiine larvae (Lepidoptera: Noctuidae) were reproducibly separated by sucrose density gradient centrifugation and fractionation. Purity of the preparations was established by electron microscopy. Virion proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); each produced 12 distinct polypeptides ranging from 10,300 to 82,900 mw. Qualitative and quantitative differences were found between most of the polypeptide patterns. The singly embedded viron (SEV)-type isolates had two major components with mw in the range of 32,900–35,200; multiply embedded virion (MEV)-type isolates had a major component of ca. 12,500 mw. SEV isolates showed almost no within-group differences, while minor differences were found among the MEV banding patterns in both intensity and presence of certain bands. Capsids and envelopes from MEV had two to four polypeptides with mw between 10,800 and 26,900. The presence of more than one polypeptide and electron microscopy of sample composition suggested that the capsid and envelope are composed of several distinct proteins. 相似文献
11.
G.F. Rohrmann S. Melgaard G.S. Beaudreau M.E. Martignoni 《Journal of invertebrate pathology》1982,40(2):237-241
Three nuclear polyhedrosis viruses isolated from larvae of the insect genus Choristoneura showed polyhedrins of 28–30,000 daltons, genome sizes of 78–82 × 106 daltons, and guanine plus cytosine contents of 47.9–49.4%. It was demonstrated by comparison of restriction endonuclease fragment patterns that two of the viruses are closely related genetically. 相似文献
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Nuclear polyhedrosis viruses isolated from the alfalfa looper,Autographa californica (Speyer) (AcMNPV), and a mint looper,Rachiplusia ou (Guenée) (RoMNPV), were assayed against the European corn borer,Ostrinia nubilalis Hübner), and applied to field corn for suppression of this insect. Fourteen days post-treatment, LC50 values were 46.50 abd 0.95×105 PIB/ml diet for AcMNPV and RoMNPV, respectively. Both viruses caused significant reductions in number of larvae per plant and in plant damage when the material was applied with a backpack sprayer. Data indicate that RoMNPV kills in less time than AcMNPV. When the viruses were applied with a commercial highboy applicator, the centimeters of damage to cornstalks was reduced by 65% with RoMNPV during the 1st generation and by 32.9% in the 2nd generation. 相似文献
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Two media supporting the growth of several established lepidopteran cell lines in monolayer and suspension culture are described. The medium designated was developed specifically as an inexpensive medium for production of cells of Spodoptera frugiperda and the homologous nuclear polyhedrosis virus (NPV) of this species. Simultaneously, a second medium was formulated in which the amino acid requirements were provided by enzymatic protein hydrolysates, one of which was termed . Several cell lines could be adapted easily to this medium. supported growth of S. frugiperda cells and production of the NPV's of S. frugiperda and Autographa californica. supported the growth of cells of S. frugiperda. Carpocapsa pomonella, Heliothis zea, and Trichoplusia ni. Cells of the latter produced the polyhedra of T. ni and A. californica NPV's in this medium. 相似文献
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Structural studies on the polyhedral inclusion bodies, virions, and DNA of the nuclear polyhedrosis virus of the cotton bollworm Heliothis zea.
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The polyhedral inclusion body of the cotton bollworm nuclear polyhedrosis virus contains virions occluded in an orthogonal crystalline matrix. The virions appear as rods or, more frequently, as oval structures that form upon bending of the nucleocapsid within the viral membrane. The nucleocapsid consists at least of DNA surrounded by a capsid composed of subunits, possibly helically arranged. The viral DNA is circular and supercoiled. It is heterogenous in size with contour lengths ranging from 15 to 45 mum. 相似文献
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Diseased Spodoptera littoralis larvae were collected from 21 different regions of Israel. Nuclear polyhedrosis viruses were isolated from these larvae, and viral DNAs were compared by restriction endonuclease analysis. Two distinct virus types, represented with approximately equal frequency, were found. Several wild isolates of each virus type exhibited minor restriction pattern differences. Plaque purification of the wild isolates revealed the presence of additional genotypic variants. 相似文献
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Inclusion bodies (IB) of nuclear polyhedrosis viruses (NPV) of Bombyx mori and Galleria mellonella were dissolved in 67% acetic acid and in sodium carbonate solution at pH 11.0. The polyhedral protein preparations obtained in this way were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by ultracentrifugation. The polyhedral proteins of B. mori and G. mellonella NPV obtained by dissolving IB in acetic acid were shown to have mainly one component with a molecular weight of about ± 28,000 and sedimentation coefficients in 0.1 M NaOH of 1.8 and 2.1 S, respectively. During dissolution of IB in alkaline condition, both the proteins are cleaved and reveal several components. The quantity and the ratio of the components depend on the duration of storing and on the temperature during the dissolution of IB and on the stage of insect development at the time of IB isolation.It is suggested that the cleavage of IB protein is a result of alkaline proteinase(s) activity. 相似文献
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Tsushima M Ikuno Y Nagata S Kodama K Matsuno T 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2002,133(3):331-336
The carotenoids of 12 species of Siluriformes fishes (eight families) were investigated from a comparative biochemical point of view. The patterns of carotenoids in catfishes belonging to the family Siluridae were quite different from those of the other seven families of catfishes (Bagridae, Amblycipitidae, Clariidae, Plotosidae, Ictaluridae, Callichthyidae and Malapteruridae). 7, 8-Dihydro-beta-carotene; 7, 8, 7', 8'- and 7, 8, 9, 10-tetrahydro-beta-carotene; (3R)-7', 8'-dihydro-beta-cryptoxanthin; 7, 8-dihydrolutein A; 7, 8-dihydrolutein B; parasiloxanthin; 7', 8'-dihydroparasiloxanthin; and 4 or 4'-hydroxyparasiloxanthin were characteristic carotenoids found in only one family, Siluridae, and these carotenoids accounted for 24-60% of total carotenoids. In catfishes belonging to the other seven families except Siluridae, the carotenoid patterns were very similar and the most predominant carotenoid was zeaxanthins (23-56%). 相似文献