HS-SPME & GC-MS分析印加果种子的挥发性物质

利用顶空固相微萃取-气相色谱质谱法(HS-SPME & GC-MS)分析印加果Plukenetia volubilis近成熟种子的挥发性物质,通过面积归一法计算出各成分的相对含量。结果共分离鉴定出64种化学成分,其中6种酯类化合物,占45.86%;12种醇类化合物,占27.71%;7种烯烃类化合物,占6.71%;9种醛类化合物,占3.89%;6种酮类化合物,占2.78%;16种烷烃类化合物,占1.78%。此外,盐酸氨基脲占5.47%,氨基脲占3.25%,还有1.51%三氯乙酸和0.23%甲基异丙基苯及其他化合物。     

Phenolic compounds from the fruit of Garcinia dulcis

Dulcinoside (1), dulcisisoflavone (2), dulcisxanthone A (3) and sphaerobioside acetate (6) together with 22 known compounds were isolated from the green fruit of G. dulcis. Dulcisflavan (4), dulcisxanthone B (5) and isonormangostin (7) together with 22 known compounds were isolated from the ripe fruit. Compounds 6 and 7 were synthetic known compounds. Their structures were determined by spectroscopic methods. The radical scavenging and antibacterial activities of some of the compounds were investigated.     

民族药四数九里香的化学成分及其细胞毒活性研究

为了阐明民族药四数九里香Murraya tetramera Huang的药效物质基础,课题组使用色谱技术从其醇提取物中分离得14个化合物;运用~1H NMR、¹³C NMR波谱方法和文献数据对比鉴定为正24烷酸(1)、9,13,17,21-tetramethyl-5-docosenoic acid(2)、3-O-β-D-吡喃葡萄糖基-山奈酚甙(3)、补骨脂素(4)、紫苏醛(5)、槲皮素(6)、methyl salicylate glucoside(7)、(9S,10R,11E,13R,15Z)-9,10,13-trihydroxyoctadeca-11,15-dienoic Acid(8)、2-isopropyl-5-methylphenol(9)、β-胡萝卜甘(10)、7-羟基香豆素(11)、七叶内酯(12)、β-谷甾醇醋酸酯(13)、山奈酚(14)。除了化合物4、6外,其他化合物为首次从该植物中分离得到。同时,研究各化合物对5株肿瘤细胞的体外生长抑制作用。与阳性对照组比较,结果表明:化合物3、4、5、6、7、8、10、11、12、13、14对白血病HL60显现良好的细胞毒活性;化合物1～14对肺腺癌A549的的细胞毒活性低于阳性对照组;化合物6、13、14对肝癌SMMC7721显现良好的细胞毒活性;化合物1、2、3、6、7、9、11、12对乳腺癌MCF7显现良好的细胞毒活性;化合物1～14对结肠癌SW480的细胞毒活性低于阳性对照组。     

A benzil and isoflavone from Iris tenuifolia

Two compounds, tenuifodione (1) and tenuifone (2), and 12 known compounds, izalpinin (3), alpinone (4), arborinone (5), irilin B (6), irisone A (7), irisone B (8), betavulgarin (9), beta-sitosterol (10), 5,7-dihydroxy-2',6-dimethoxyisoflavone (11), 2',5-dihdroxy-6,7-methylenedioxy flavanone (12), irisoid A (13) and ethyl-beta-d-glucopyranoside (14) were isolated from the whole plant of Iris tenuifolia Pall. All compounds, except 12, were isolated for the first time from this plant. Compounds 2, 3 and 11 have shown a considerable DPPH radical scavenging activity. Structures of these compounds were identified on the basis of spectroscopic techniques, including 2D NMR. Compounds 3, 5 and 7 were also subjected to single-crystal X-ray diffraction analysis and their structures were unambiguously deduced.     

潞西山龙眼的化学成分研究

从山龙眼属植物潞西山龙眼的叶子中分离了6个化合物,利用核磁共振等方法,鉴定为熊果苷(1)、ro-bustaside B(2)、robustaside D(3)、β-谷甾醇(4)、helicide(5)和(E)-(4-羟基苯基)-丙烯酸甲酯(6)。这6个化合物均为首次从该植物中分离得到。     

Synthesis of Schiff bases of 2-amino-5-aryl-1,3,4-oxadiazoles and their evaluation for antimicrobial activities

Twenty Schiff bases of 2-amino-5-aryl-1,3,4-oxadiazoles have been synthesized with different aromatic aldehydes. The structures of the compounds were confirmed by nitrogen analysis, IR and 13C-NMR spectral data. The antibacterial properties of the compounds were investigated against Proteus mirabilis (MTCC-425), Pseudomonas aeruginosa (MTCC-424), Bacillus subtilis (MTCC-619) and Staphylococcus aureus (MTCC-96) using the broth dilution method. The most active compounds were 4c (64 microg/ml), 4f (68 microg/ml), 4m (64 microg/ml) and 4q (62 microg/ml). The antifungal screening of the compounds were carried out using Aspergillus niger (MTCC-1344) and Candida albicans (MTCC-227) using the broth dilution method. Active compounds were 4g (52 microg/ml), 4h (56 microg/ml), 4l (60 microg/ml), 4m (58 microg/ml).     

大瘤足蕨和镰羽瘤足蕨的化学成分研究

对瘤足蕨属中的大瘤足蕨和镰羽瘤足蕨进行化学成分研究,从这两种植物中分离并鉴定了9个化合物,分别是:大波斯菊苷(1)、异山奈甲黄素(2)、astragalin(3)、3β,27-dihydroxylup-12-ene(4)、hydroxyhopanone(5)、plagiogyrin a(6)、androsin(7)、β-谷甾醇(8)、β-胡萝卜苷(9).所有化合物均为首次从这两种植物中分离得到,其中化合物1、4、7、9为这两种植物的共有成分.     

苦玄参的化学成分研究

对广西传统的抗菌消炎药用植物苦玄参进行了化学成分研究,采用柱色谱进行分离纯化,运用波谱法进行了结构解析,共鉴定得到7个化合物。它们分别为:芹菜素(1)、芹菜素-7-O-β-D-葡萄糖酸(2)、芹菜素-7-O-α-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖酸(3)、迷迭香酸(4)、苦玄参苷Ⅳ(5)、苦玄参苷Ⅹ(6)、阿克替苷(7)。其中化合物2、3、4为首次从该植物中分离得到。     

Essential oils from two endemic species of Apiaceae from Iran

The composition of essential oils of Leutea glaucopruinosa (Rech.f.) Akhani & Salimian comb. nov., and Zeravschania (Boiss. & Hausskn.) Salimian & Akhani comb. nov. were analysed by GC-MS. 49 compounds are identified in the former and 33 compounds in the latter, comprising a total of 76 compounds in both species. Both species were originally described under Peucedanum, which are transferred in this paper into Leutea and Zeravschania, respectively. The chemical compounds of the essential oils show that there are only seven common compounds between two species. The major compounds of L. glaucopruinosa are mostly monoterpene hydrocarbons and oxygenated monoterpenes, in which alpha-pinene (31.5%), sabinene (9.7%), beta-pinene (9.2%), exo-fenchyl acetate (4.5%) are dominant. In Z. pastinacifolia sesquiterpene hydrocarbons and phenylpropanoids dominate with beta-bisabolene (37.3%), 3,1-butyl-1,2-dimethoxy benzene (14.9%), 10,11-dimethylbicyclo[6.3.0]undec-(8)-en-9-one (12.9%), 4-t-butyl-1,2-dimethoxy benzene (6.8%), (E)-asarone (5.1%) and elemicine (4.1%) as major compounds.     

四种不同方法提取沙枣花挥发物的成分分析

为了探究不同提取方法对沙枣花挥发物化学成分分析的影响,本文采用水蒸气蒸馏法、固相微萃取法、顶空采集法和气囊采集法提取沙枣(Elaeagnus angustifolia)花的挥发性物质,并利用气相色谱-质谱联用仪(GC-MS)分别对4种方法提取的挥发物质进行化学成分分析鉴定。从4种方法提取的沙枣花挥发物质中共检测出51种化合物,其中酯类14种、烷类13种、酚类9种、酮类3种、醇类4种、其它物质8种。顶空收集法、水蒸气提取法、气囊采集法和固相微萃取法提取的挥发物质中分别检出化合物13种、19种、22种和18种,并且用2种(或2种以上)方法提取的沙枣花挥发物质中相同化合物有15种(即:酯类6种、烷类5种、酚类2种、醇类1种、其它物质1种),分别为:反式-肉桂酸乙酯、顺式-肉桂酸乙酯、反式-肉桂酸甲酯、邻苯二甲酸二甲酯、正癸烷、正十二烷、正十四烷、正十六烷、正二十烷、愈创木酚、4,4'-亚甲基双(2,6-二叔丁基苯酚)、2-苯基-1-丙烯、乙酸叶醇酯、肉桂酸异丙酯和苯乙醇。4种方法提取的沙枣花挥发物中均含反式-肉桂酸乙酯和邻苯二甲酸二甲酯2种化合物,且反式-肉桂酸乙酯的含量较高,分别为83.75%(水蒸气蒸馏法)、14.45%(固相微萃取法)、78.27%(顶空收集法)和56.65%(气囊采集法)。4种方法中气囊采集法提取的沙枣花挥发物质中所含化合物种类最多(22种),而且操作最简单,因此气囊采集法是提取沙枣花挥发物的较好方法。     

Cycloartane type triterpenoids from the rhizomes of Polygonum bistorta

Two new compounds, 24(E)-ethylidenecycloartanone (1) and 24(E)-ethylidenecycloartan-3alpha-ol (2) were isolated from the rhizomes of Polygonum bistorta, together with seven known compounds viz., cycloartane-3,24-dione (3), 24-methylenecycloartanone (4), gamma-sitosterol (5), beta-sitosterol (6), beta-sitosterone (7), friedelin (8) and 3beta-friedelinol (9). All the cycloartane type triterpenoids, compounds 7 and 8 are reported for the first time from this plant. A combination of 1D and 2D NMR spectroscopy and MS were mainly used to elucidate the structures of the new compounds 1 and 2.     

红背山麻杆化学成分的研究

采用柱色谱技术从红背山麻杆叶子的60％乙醇提取物中分离得到4个黄酮苷和2个其他类化合物.根据理化性质及波谱方法分别鉴定为:芹菜素-6-C-D-葡萄糖苷(1)、芹菜素-7-O-芸香糖苷(2)、芹菜素-7-O-β-(2″-O-α-鼠李糖基)葡萄糖醛酸苷(3)、木犀草素-7-O-α-L-鼠李糖(1→6)-β-D-葡萄糖苷(4)、没食子酸乙酯(5)、β-胡萝卜苷(6).以上化合物均为首次从该植物中分离得到,其中化合物1～4为首次从山麻杆属中分离得到的黄酮苷.     

金花茶花化学成分的研究

采用反复硅胶柱色谱法、Sephadex LH-20柱色谱法、ODS柱色谱法、反复重结晶等方法对金花茶花的化学成分进行分离纯化,并通过理化常数测定和波谱分析等方法进行结构鉴定.从金花茶花的乙醇提取物中分离得到13个化合物,分别鉴定为:槲皮素(1)、槲皮素-7-O-β-D-葡萄糖苷(2)、槲皮素-3-O-β-D-葡萄糖苷(...     

腐婢根化学成分研究

从马鞭草科腐婢属植物腐婢(Premna microphylla)的根中分离鉴定出7种化合物,其结构分别为β-谷甾醇(1).棕榈酸(2).反式-对甲氧基肉桂酸(3).丁香色酮(4).木犀草素(5).木犀草素-7-O-β-D-葡萄糖甙(6)和胡麻素(7).这些成分均为首次从腐婢根中分离得到.     

Isolation of chlorogenic acids and their derivatives from Stemona japonica by preparative HPLC and evaluation of their anti-AIV (H5N1) activity in vitro

Two chlorogenic acids and five chlorogenic acid derivatives were simultaneously separated and purified from Stemona japonica by preparative high-performance liquid chromatography. Five of the collected compounds were over 95% pure while the other two compounds were over 90% pure. Their structures were elucidated as 3-O-feruloylquinic acid (1), 4-O-feruloylquinic acid (2), methyl 3-O-feruloylquinate (3), methyl 5-O-caffeyolquinate (4), methyl 4-O-feruloylquinate (5), ethyl 3-O-feruloylquinate (6) and the new compound ethyl 4-O-feruloylquinate (7) by UV, NMR and ESI-MS. All compounds were obtained from Stemona species for the first time, however compounds 6 and 7 are believed to be artefacts from the ethanol extraction. The anti-AIV (H5N1) activities were evaluated by Neutral Red uptake assay. Compounds 3 and 4 exerted moderate inhibitory effect against AIV (H5N1) in vitro.     

扛板归根化学成分研究

从扛板归(Polygonum perfoliatum)根中分离鉴定出7种化合物,其结构分别为β-谷甾醇(1)、反式-对羟基肉桂酸甲酯(2)、槲皮素(3)、槲皮素-3-O-β-D-葡萄糖甙(4)、大黄素(5)、大黄素甲醚(6)和芦荟大黄素(7)。这些成分均为首次从扛板归根中分离得到。     

Chemical constituents from the whole herbs of Peperomia tetraphylla and their chemotaxonomic significance

Our studies report the isolation of seventeen compounds (1–17), which include three phenolic acids (12–14), seven flavonoids (3, 6, 8, 11, 15–17), four secolignans (1, 2, 5 and 9), one tetrahydrofuran lignin (10) and two alkaloids (4 and 7). These compounds have been obtained from the Peperomia tetraphylla using chromatographic methods. Their structures were confirmed based on spectroscopic methods. Among them, five compounds (4, 7, 8, 11 and 15) were found in the family, four compounds (3 and 12–14) were firstly reported from genus Peperomia, and four compounds (6, 9, 16 and 17) were firstly obtained from this species. Furthermore, the chemotaxonomic significance of these ingredients from the whole plants of P. tetraphylla has been discussed in detail.     

晶帽石斛的化学成分研究

该研究利用硅胶、凝胶、MCI、中压制备色谱(MPLC)和高效液相半制备色谱(semi-HPLC)等方法,对晶帽石斛(Dendrobium crystallinum)进行了化学成分研究。结果表明:提纯、分离共得到10个化合物,经波谱数据分析及与文献数据对照,分别鉴定为石斛酚(1),3,4'-二羟基-5-甲氧基联苄(2),3,4',5-三羟基-3-甲氧基联苄(3),二氢藜芦醇(4),安告佛醇(5),3',5,7-三羟基-4'-甲氧基黄烷酮(6),4',5,7-三羟基-6-甲氧基黄烷酮(7),丁香树脂醇(8),β-谷甾醇(9),β-胡萝卜苷(10)。其中,除化合物2和化合物10以外,其余8个化合物均在该种植物中首次发现。     

小茴香根的化学成分研究

从小茴香(Foeniculum vulgare Mill.)根的乙醇提取物中分离得到了5个化合物,通过MS和NMR等方法鉴定为:莳萝脑(1),亚油酸蔗糖苷(2),镰叶芹二醇(3),β-谷甾醇(4),豆甾醇-β-D-吡喃葡萄糖苷(5),其中化合物2和3首次从该种植物中分离得到。