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1.
S. Kukushkin A. Kanshina A. Timina T. Baybikov V. Mikhalishin 《European Journal of Wildlife Research》2008,54(3):515-518
Samples of blood sera and internal organs were collected from 90 shot wild boars (Sus scrofa) in five regions of Russian Federation. Blood sera were tested for antibodies against porcine reproductive and respiratory
syndrome virus (PRRSV) using enzyme-linked immunosorbent assay (ELISA). In addition, samples of internal organs (lungs, lymph
nodes, spleen) were tested by polymerase chain reaction (nested PCR) for PRRSV antigen. The result of our investigation showed
that all samples were negative. However, PRRSV is widespread in domestic swine throughout Russia including the examined regions.
Since the results show the absence of PRRSV infection in wild boars in the five examined regions of Russia, wild boars seem
not to play any role in the epidemiology of PRRSV in Russia. 相似文献
2.
Christoph G. Baums Gerd Josef Verkühlen Thomas Rehm Luciana M. G. Silva Martin Beyerbach Klaus Pohlmeyer Peter Valentin-Weigand 《Applied microbiology》2007,73(3):711-717
Invasive serotype 2 (cps2+) strains of Streptococcus suis cause meningitis in pigs and humans. Four case reports of S. suis meningitis in hunters suggest transmission of S. suis through the butchering of wild boars. Therefore, the objective of this study was to investigate the prevalence of potentially human-pathogenic S. suis strains in wild boars. S. suis was isolated from 92% of all tested tonsils (n = 200) from wild boars. A total of 244 S. suis isolates were genotyped using PCR assays for the detection of serotype-specific genes, the hemolysin gene sly, and the virulence-associated genes mrp and epf. The prevalence of the cps2+ genotype among strains from wild boars was comparable to that of control strains from domestic pig carriers. Ninety-five percent of the cps2+ wild boar strains were positive for mrp, sly, and epf*, the large variant of epf. Interestingly, epf* was significantly more frequently detected in cps2+ strains from wild boars than in those from domestic pigs; epf* is also typically found in European S. suis isolates from humans, including a meningitis isolate from a German hunter. These results suggest that at least 10% of wild boars in Northwestern Germany carry S. suis strains that are potentially virulent in humans. Additional amplified fragment length polymorphism analysis supported this hypothesis, since homogeneous clustering of the epf* mrp+ sly+ cps2+ strains from wild boars with invasive human and porcine strains was observed. 相似文献
3.
Yoshihiro Shimoji Makoto Osaki Yohsuke Ogawa Kazumasa Shiraiwa Sayaka Nishikawa Masahiro Eguchi Takehisa Yamamoto Toshiyuki Tsutsui 《Microbiology and immunology》2019,63(11):465-468
The potential role of wild boars as a source of erysipelas infection was investigated. An ELISA test of wild boar serum samples from 41 prefectures in Japan revealed that proportions of the Erysipelothrix rhusiopathiae‐positive samples were very high in all the prefectures, and the mean positive rate was 95.6% (1312/1372). Serovars of E. rhusiopathiae isolates from wild boars were similar to those of previously reported swine isolates, and all serovar isolates tested were found to be pathogenic to mice. These results suggest that wild boars in Japan constitute a reservoir of E. rhusiopathiae and may pose risks to other animals. 相似文献
4.
C. Köppel L. Knopf M.-P. Ryser R. Miserez B. Thür K. D. C. Stärk 《European Journal of Wildlife Research》2007,53(3):212-220
The large abundance of free-ranging wild boars (Sus scrofa) and a trend towards animal friendly outdoor management of domestic pigs lead to an increasing probability of disease transmission
between those animal populations. In 2001, an active monitoring was started for classical swine fever (CSF), Aujeszky’s disease
(AD) and porcine brucellosis (PB) in wild boars in Switzerland. The objective of this programme was to document the serological
status of wild boars regarding the selected pathogens. To continue this serosurveillance, 1,060 wild boar samples were collected
during two regular hunting seasons in 2004–2005. Furthermore, in a pilot study, 61 outdoor pigs from 14 farms located in areas
with high wild boar densities were sampled in 2004 and serologically tested for AD and PB. All wild boar samples were negative
for CSF. Seroprevalence for AD was 2.83% (95% CI 1.91–4.02%). Seroprevalence for PB was 13.5% (95% CI 10.7–16.7%) for the
Rose Bengal test and 11.05% (95% CI 8.82–13.61%) for the indirect ELISA. There was no serological evidence for AD in domestic
pigs. All tested animals from 13 piggeries were seronegative for PB, but three pigs from the same farm showed doubtful results.
Further investigations on the farm did not indicate the presence of PB in the herd. These findings urge the need for better
diagnostic tools to obtain reliable results concerning PB prevalence. Since contact and following transmission of infectious
agents between infected wild boars and outdoor pigs might occur in the future, it is advisable to include outdoor pigs in
areas at risk in routine surveillance programmes. 相似文献
5.
Dolors Vidal Victoria Naranjo Rafael Mateo Christian Gortazar José de la Fuente 《European Journal of Wildlife Research》2006,52(4):301-304
Bovine tuberculosis (bTB) caused by Mycobacterium bovis (Mycobacterium tuberculosis complex) is a zoonotic disease that affects cattle and wildlife worldwide. European wild boar (Sus scrofa) is a major reservoir host of M. bovis in south-central Spain. The identification of biomarkers to predict bTB in wild boars by dependable methods that do not require
killing the host would greatly contribute to the implementation of effective control programs for bTB in this region. In this
study, we have characterized serum biochemical values in European wild boars in Spain to determine whether biochemical parameters
in the serum varied significantly with the presence of bTB in this species. Although apolipoprotein A1 and IgG levels were
higher in uninfected boars, the results did not support good predictive value for serum biochemical parameters studied for
European wild boars in relation to bTB in Spain. 相似文献
6.
Hammer R Ritzmann M Palzer A Lang C Hammer B Pesch S Ladinig A 《Journal of wildlife diseases》2012,48(1):87-94
Samples were collected from 203 wild boars (Sus scrofa) hunted in Baden-Wurtemburg, Germany from November-January 2008 and 2009. Samples from the lung and tonsil were analyzed by quantitative polymerase chain reaction (qPCR) for porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European type) and type 2 (American type). A qPCR to detect porcine circovirus type 2 (PCV2)-specific genome was performed on tissue homogenates including lung, tonsils, and inguinal lymph nodes. Serum samples were tested for antibodies against PRRSV and PCV2 by enzyme-linked immunosorbent assay (ELISA). No PRRSV was detected in any of the 203 samples and one sample had detectable antibodies against PRRSV. We detected PCV2 in organ materials from 103 wild boars with a prevalence of 50.7%. The number of wild boars positive for PCV2 by PCR varied according to the population density of wild boars among woodlands. More positive samples were detected in woodlands with a high density of wild boars. We found no correlation between the number of PCV2-positive wild boars and the density of domestic pigs in the surrounding area. The number of wild boars positive for antibodies against PCV2 by the INGEZIM Circovirus IgG/IgM test kit was low (53 sera positive for IgG- and three sera positive for IgM-antibodies) in comparison to the higher positive results from the INGEZIM CIRCO IgG test kit (102 positive and 12 inconclusive results). 相似文献
7.
【目的】猪链球菌(Streptococcus suis)是猪的重要病原菌,同时也是人畜共患病原。猪的扁桃体是猪链球菌主要定殖部位之一,是易感猪和人的重要传染源。因此,对屠宰场健康猪进行猪链球菌流行病学调查,具有重要的公共卫生学意义。【方法】本研究自2020年至2021年,从浙江某市屠宰场采集健康猪扁桃体样品,分离鉴定猪链球菌,采用血清型特异性PCR法分型,通过耐药基因检测、药敏试验、斑马鱼毒力实验分析其耐药及致病特征。【结果】131份健康猪扁桃体样品猪链球菌阳性率为62.59%(82/131),共分离猪链球菌68株,其中16型分离率最高,占比16.18%(11/68),其次为31型(11.76%,8/68)、9型(7.35%,5/68)、3型(7.35%,5/68)等。含2种及以上血清型的扁桃体样品占15.85%(13/82)。药敏试验表明,分离株主要对林可酰胺类(100%,68/68)、大环内酯类(98.53%,67/68)、四环素类(100%,68/68)抗生素耐药,所有菌株均属于多药耐药。值得关注的是,有18株菌对青霉素耐药、3株菌对头孢噻肟耐药、2株菌对利福平耐药、11株菌对利... 相似文献
8.
Mario Chiari Nicola Ferrari Mariagrazia Zanoni Loris Alborali 《European Journal of Wildlife Research》2014,60(2):187-192
Mycoplasma hyopneumoniae (Mhyo) is the principal etiological agent of enzootic pneumonia (EP), one of the most economically important diseases in the pig production industry worldwide. Although swine and wild boars (Sus scrofa) share susceptibility to Mhyo infection, information regarding the dynamics of Mhyo infections and pathology in wild boars is currently limited. Therefore, the aim of this study was to determine the spatiotemporal dynamics of Mhyo infections in free-living wild boars through serological ELISA testing. Additionally, the presence of EP-like gross lesions and their association with the presence of Mhyo, based on a PCR assay, were assessed. Over a period of 5 years, antibodies against Mhyo were detected in 655 (30.0 %) of the 2,177 analyzed wild boars. A generalized temporal increase of seroprevalence coupled with an increase of mean antibody titers of seropositive individuals was found. Moreover, a similar seroprevalence between age classes associated with higher antibody titers of younger individuals indicated the wild boars were infected during the early stages of their life. Out of 99 lungs tested, 43 showed EP-like lesions and 45 were PCR positive for the presence of Mhyo DNA. The lung lesion scores were related to the sex and age of the wild boars, with young individuals having higher lung scores than others, and there was a positive association with the Mhyo PCR-positive status. The temporal increase of the Mhyo seroprevalence and infection association with EP-like lesions, when coupled with the spreading of wild boar populations, raises concerns on the epidemiological role of this species. 相似文献
9.
G. Vengust R. Lindtner-Knific D. Zele A. Bidovec 《European Journal of Wildlife Research》2008,54(4):749-752
Serum samples collected from 437 shot wild boars (Sus scrofa) were tested for the presence of antibodies against Leptospira interrogans sensu lato in wild boar in Slovenia. Assessment of leptospira-specific antibodies was performed by microscopic agglutination
test. Antibodies against at least one of the pathogenic serovars were detected in 200 (45.8%) sera. From 200 positive samples,
100 samples (50%) had positive titre against a single serovar, while 100 (50%) samples had positive titres against two or
more serovars. The most frequently detected antibodies were those against serovar Tarassovi. This investigation confirmed
the presence of different pathogenic serovars in wild boar across Slovenia. It can be concluded that wild boars are natural
reservoirs of at least some of the leptospiral serovars that represent a potential source of leptospirosis for other wild
and domestic animals, as well as for humans. 相似文献
10.
Matsumoto J Kako Y Morita Y Kabeya H Sakano C Nagai A Maruyama S Nogami S 《Parasitology international》2011,60(3):331-332
The ingestion of undercooked meat from wild animals can be a source of Toxoplasma gondii infection in humans and other animals. In this study, we determined the seroprevalence of T. gondii infection in 175 wild boars (Sus scrofa leucomystax) and 107 wild sika deer (Cervus nippon) hunted in 2004–2007 in Gunma Prefecture, Japan, by using a commercial latex agglutination test (LAT). Antibodies (LAT, 1:64 or higher) to T. gondii were found in 6.3% of wild boars and 1.9% of sika deer. This is the first record of T. gondii infection in wild deer in Japan, and deer and wild boar meat should be cooked well before human consumption. 相似文献
11.
【目的】猪链球菌是一种能感染人和猪的人畜共患病病原,并且还可零星感染多种哺乳动物。本试验旨在调查流浪猫携带猪链球菌的情况。【方法】在流浪猫身上分离猪链球菌,经血清凝集实验和PCR检测,鉴定其血清型;经多序列位点分型分析,鉴定其ST型;将所分离的细菌与Gen Bank上已公布的猪链球菌构建16S rRNA的系统发育树,分析该菌株与其他猪链球菌的亲缘关系;药敏纸片法分析其耐药性;小鼠攻毒试验分析其毒力。【结果】本试验在流浪猫身上分离到一株猪链球菌,命名为m70,其血清型为9型。多序列位点分型显示,m70株属于一个新的ST型。与Gen Bank上已公布的猪链球菌16S rRNA进行系统发育树分析,结果显示m70属于一个单独的分支。m70与临床菌株的耐药情况相似,对四环素耐药,对红霉素中介耐药,对氨苄西林敏感。小鼠攻毒试验显示,感染10~8 CFU剂量m70的小鼠,死亡率达到60%–80%(3/5–4/5),3次攻毒试验的平均LD(50)为5.1×107 CFU;而本实验室保存的猪链球菌强毒株HA9801感染小鼠的平均LD(50)为3.9×107 CFU,两者之间没有显著差异(P〈0.05)。【结论】从流浪猫身上分离得到的猪链球菌m70属于优势血清型,且毒力较强,提示一些流行血清型的猪链球菌强毒株具有从流浪猫传染人的潜在风险。 相似文献
12.
Simon Gubbins Charlotte J Cook Kieran Hyder Kay Boulton Carol Davis Eurion Thomas Will Haresign Stephen C Bishop Beatriz Villanueva Rachel D Eglin 《BMC veterinary research》2009,5(1):1-8
Background
Control of brucellosis in livestock, wildlife and humans depends on the reliability of the methods used for detection and identification of bacteria. In the present study, we describe the evaluation of the recently established real-time PCR assay based on the Brucella-specific insertion sequence IS711 with blood samples from 199 wild boars (first group of animals) and tissue samples from 53 wild boars (second group of animals) collected in Switzerland. Results from IS711 real-time PCR were compared to those obtained by bacterial isolation, Rose Bengal Test (RBT), competitive ELISA (c-ELISA) and indirect ELISA (i-ELISA). 相似文献13.
V. Kaden E. Lange A. Hänel A. Hlinak L. Mewes G. Hergarten B. Irsch J. Dedek W. Bruer 《European Journal of Wildlife Research》2009,55(2):153-159
The objective of this study was to retrospectively evaluate the occurrence of porcine parvovirus (PPV), Aujeszky’s disease
virus (ADV), transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCV), porcine reproductive and
respiratory syndrome virus (PRRSV) and swine influenza virus (SIV) in selected wild boar populations in Germany (n
= 1,221). Commercial enzyme linked immunosorbent assay and hemagglutination inhibition tests were used for serological monitoring.
The serosurvey revealed seroprevalence rates of 64.28%, 11.26%, 7.87%, 7.84%, 3.82% and 1.59% for PPV, ADV, PRCV, SIV, PRRSV
and TGEV, respectively. The seroprevalence rates differed between populations and age classes with the highest number of antibody-positive
wild boars in older animals (>1 year old). No antibodies to TGEV were found in Baden–Wuerttemberg and in Mecklenburg–Western
Pomerania (investigation period 1997/1998). In addition, sera collected in Mecklenburg–Western Pomerania in 1997/1998 were
negative for SIV. Even though the seroprevalence rates established for these viruses, except for PPV, were relatively low,
wild boars may act as a reservoir for pathogens and a source of infection for domestic pigs and humans. Based on the epidemiological
situation, no risk of a spread of these viruses should emanate from wild boars, neither for wildlife nor for livestock. However,
effective and science-based disease monitoring programmes should continuously be carried out in wild boar populations. 相似文献
14.
Hye-Jung Lee Ok-Sik Chung Jae-Lip Kim Seung-Ha Lee Young-Bok Yoo Min Seo 《The Korean journal of parasitology》2015,53(2):233-236
A total 7 outbreaks of trichinellosis have occurred in Korea, mostly as a result of consumption of raw wild boar (Sus scrofa) meat. Since only 1 serological survey on wild boars had yet been performed in Korea, the present study aimed to estimate the prevalence of trichinellosis in wild boars and some species of rodents by artificial digestion and serological examinations in Yanggu-gun, Gangwon-do, the endemic area of trichinellosis. Both the wild boar and rodent muscle samples revealed no Trichinella larvae by direct examination and artificial digestion method. However, serological examinations revealed that 4 wild boar sera samples out of 118 (3.4%) were positive to Trichinella antigen. Although the recovery of Trichinella larvae ended in a failure, it is proved for the first time that the sylvatic cycle of Trichinella has been maintained in wild boars of Gangwon-do, Korea. 相似文献
15.
Seung Won Kang Huong Thi Thanh Doan Jin Hyeong Noh Se Eun Choe Mi Sun Yoo Young Ha Kim Kondreddy Eswar Reddy Thuy Thi Dieu Nguyen Dong Van Quyen Lien Thi Kim Nguyen Chang Hee Kweon Suk Chan Jung 《Parasitology international》2013,62(6):583-585
Toxoplasma gondii and Trichinella spiralis are important zoonotic pathogens with worldwide distributions. In Korea, several outbreaks of human toxoplasmosis and trichinellosis due to the consumption of infected wild animals have been reported. The purpose of this study was to determine the seroprevalence of T. gondii and T. spiralis infections in wild boars killed in Korea from December 2009 to October 2011. A total of 521 wild boars hunted in eight provinces were examined for antibodies to T. gondii and T. spiralis by using commercial ELISA kits. Overall, 25.1% of serum samples from individual boars were seropositive for T. gondii and 1.7% were seropositive for T. spiralis. Seropositive for T. gondii was found in the boars in all the eight provinces investigated and for T. spiralis in four provinces. This is the first report on the seroprevalence of T. gondii and T. spiralis infections in wild boars in Korea. The consumption of undercooked wild boar meat may expose humans to a high risk of infection. 相似文献
16.
Gerald Reiner Melina Winkelmann Hermann Willems 《European Journal of Wildlife Research》2011,57(3):443-448
Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli are important pathogens in domestic pig production, responsible for porcine intestinal adenomatosis, swine dysentery, and
porcine intestinal spirochetosis, respectively. They are widely distributed among pig-producing units around the world, and
transmission is accomplished by relatively weak immunity, long shedding intervals, sequential shedding, and actual environmental
survival. Little information is available on occurrence, prevalence, and quantity of these pathogens in free-ranging wild
boars. The aim of the present study was to evaluate L. intracellularis, B. hyodysenteriae, and B. pilosicoli infections in wild boars in Germany. Tissue samples from ileocaecal mucosa of 165 wild boars from 18 hunting grounds situated
in 14 of the 16 federal states of Germany were examined by conventional PCR and quantified by multiplex real-time PCR. None
of the wild boars did show any gross pathological signs of enteritis. The overall prevalence for L. intracellularis, B. hyodysenteriae, and B. pilosicoli was 20.6%, 2.4%, and 12.1%, respectively. None of the three agents was detected in 68.5% of the wild boars and in 11.1% of
the hunting grounds. Numbers of bacteria per sample were below the limit of quantification (100 cells/PCR reaction). This
is the first study on L. intracellularis and Brachyspira spp. in free-ranging wild boars. The study revealed colonised animals without signs of disease. The meaning of these findings
remains unclear, and we do not know whether and to what extent these three pathogens are exchanged between wild boars and
domestic pigs. Further research is needed to get insight into the epidemiological impact of the results. 相似文献
17.
Pedro Fernández-Llario 《Journal of Ethology》2005,23(1):9-14
The wild boar (Sus scrofa) is a medium-sized ungulate that produces a large variety of marks. Traditionally, wallowing is an activity that has fundamentally been related to several processes such as thermoregulation, reduction in the number of ectoparasites or disinfection of wounds in the skin of males resulting from fights to reach females. In this study, I analysed another possible function of wallowing in free-ranging wild boar populations when temperatures are low and parasitic numbers are not significant. The results indicate that during the sampling time, from October to February, the number of wild boars with a mud layer on their skin was 47, out of a total of 558 wild boar analysed. Of these 47 wild boars, 44 were males and within this group, 40 individuals were adult males. The wallowed males were bigger and older than non-wallowed males hunted at the same time. In addition, I found that males wallowed mainly during autumn (90.9% of all cases). In the total number of wild boars analysed, I did not notice individuals with wounds resulting from fights other than from the hunting day. On the other hand, the analysis of the females reproductive systems indicated that the rut period took place during the last days of October and the first days of November. From these results, the coincidence of the rut period with the maximum of wallowing in adult males could be indicative of a sexual function of this activity. 相似文献
18.
Yoshikazu Fujimoto Hideya Inoue Makoto Ozawa Aya Matsuu 《Microbiology and immunology》2019,63(12):517-522
We conducted a serological survey to detect antibodies against influenza A virus (IAV) in Japanese wild boars in Kagoshima prefecture, Japan, between 2014 and 2017. Seroprevalence against a pandemic‐like swine H1N1 (H1N1pdm) virus was identified in 27.1% of specimens, and 1.7% were positive for both swine H1N2 and H3N2 viruses, indicating that wild boars could play an important role in the dynamics of H1N1pdm viral dispersion in the wild. The high frequency of positive results for sera against the H1N1pdm virus suggests that cross‐species IAV transmission between wild boars, livestock, and humans is a threat to veterinary and public health. 相似文献
19.
【目的】调查猪种布鲁氏菌的基因多态性和分子流行病学特征。【方法】用经典分型方法对菌株的生物型进行鉴定,分析菌株的地理分布特点;用MLVA-16分型方法对60株猪种布鲁氏菌进行基因分型,采用在线软件评估分型方法的分辨率和位点的多态性,用BioNumerics 5.0软件进行聚类分析。【结果】我国流行的猪种布鲁氏菌主要是猪种生物1型(33株)、2型(3株)和3型菌(24株);分布范围较广,包括广东、广西、内蒙古、北京、吉林、宁夏和西藏等地。MLVA-16分型方法对猪种布鲁氏菌具有极高的分辨力,多态性指数为0.992;Panel1、MLVA-11和Panel 2B均具有较高的分辨率,多态性指数分别为0.884、0.916和0.979。60株猪种布鲁氏菌聚为6大类52个基因型,5个共享基因型(GT24,GT25,GT26,GT28,GT29)包括13株布鲁氏菌,各基因型菌株间有潜在的流行病学关联,可能是分别来自相同传染源的暴发流行;另47株布鲁氏菌呈现独特的基因型,表明菌株来自无流行病学关联的零星散发病例。猪种布鲁氏菌的最小生成树表明我国菌株分别与美国、法国和波兰的菌株有完全相同的MLVA-15基因型。【结论】中国猪种布鲁氏菌有较高的遗传多态性,并与美国、法国和阿根廷的菌株有较高的遗传相似性。我国猪种布病以零星散发为主。 相似文献
20.
Katarina Stefanidesova Elena Kocianova Vojtech Boldis Zina Kostanova Pavel Kanka Danka Nemethova Eva Spitalska 《European Journal of Wildlife Research》2008,54(3):519-524
The purpose of this study was to investigate the role of wild animals for Anaplasma phagocytophilum, other ehrlichiae/anaplasmae, Rickettsia helvetica and other rickettsiae and whether different genetic variants of A. phagocytophilum in central Slovakia exist. A total of 109 spleen samples from 49 red deer (Cervus elaphus), 30 roe deer (Capreolus capreolus), 28 wild boar (Sus scrofa) and two mouflon (Ovis musimon) were collected from June 2005 to December 2006. Polymerase chain reaction (PCR) amplification of the16S rRNA gene was used
for detection of ehrlichiae/anaplasmae. A nested PCR targeting part (392 bp) of groESL gene was applied for the specific detection of A. phagocytophilum. Fragments of the gltA and ompA genes (381 bp and 632 bp, respectively) were amplified to detect rickettsiae, followed by sequencing. A. phagocytophilum and R. helvetica were detected in wild animals. The prevalence of A. phagocytophilum was 50.0 ± 18.2% in roe deer and 53.1 ± 14.1% in red deer. None of the 28 wild boar was PCR positive for ehrlichiae/anaplasmae.
A. phagocytophilum was detected in one mouflon. R. helvetica was found in one roe deer. Our study suggests a role of cervids as a natural reservoir of A. phagocytophilum in Slovakia. However, the role of cervids and wild boars in the circulation of R. helvetica remains unknown. The analysis of sequence variation in the msp4 coding region of A. phagocytophilum showed the presence of different variants previously described in ruminants. 相似文献