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1.
Construction of Agropyron Gaertn. genetic linkage maps using a wheat 660K SNP array reveals a homoeologous relationship with the wheat genome 下载免费PDF全文
Shenghui Zhou Jinpeng Zhang Yonghe Che Weihua Liu Yuqing Lu Xinming Yang Xiuquan Li Jizeng Jia Xu Liu Lihui Li 《Plant biotechnology journal》2018,16(3):818-827
Agropyron Gaertn. (P genome) is a wild relative of wheat that harbours many genetic variations that could be used to increase the genetic diversity of wheat. To agronomically transfer important genes from the P genome to a wheat chromosome by induced homoeologous pairing and recombination, it is necessary to determine the chromosomal relationships between Agropyron and wheat. Here, we report using the wheat 660K single nucleotide polymorphism (SNP) array to genotype a segregating Agropyron F1 population derived from an interspecific cross between two cross‐pollinated diploid collections ‘Z1842’ [A. cristatum (L.) Beauv.] (male parent) and ‘Z2098’ [A. mongolicum Keng] (female parent) and 35 wheat–A. cristatum addition/substitution lines. Genetic linkage maps were constructed using 913 SNP markers distributed among seven linkage groups spanning 839.7 cM. The average distance between adjacent markers was 1.8 cM. The maps identified the homoeologous relationship between the P genome and wheat and revealed that the P and wheat genomes are collinear and relatively conserved. In addition, obvious rearrangements and introgression spread were observed throughout the P genome compared with the wheat genome. Combined with genotyping data, the complete set of wheat–A. cristatum addition/substitution lines was characterized according to their homoeologous relationships. In this study, the homoeologous relationship between the P genome and wheat was identified using genetic linkage maps, and the detection mean for wheat–A. cristatum introgressions might significantly accelerate the introgression of genetic variation from Agropyron into wheat for exploitation in wheat improvement programmes. 相似文献
2.
Z. Li S. Rahman B. Kosar-Hashemi G. Mouille R. Appels M. K. Morell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1208-1216
A cDNA clone, and a corresponding genomic DNA clone, containing full-length sequences encoding wheat starch synthase I, were
isolated from a cDNA library of hexaploid wheat (Triticum aestivum) and a genomic DNA library of Triticum tauschii, respectively. The entire sequence of the starch synthase-I cDNA (wSSI-cDNA) is 2591 bp, and it encodes a polypeptide of 647
amino-acid residues that shows 81% and 61% identity to the amino-acid sequences of SSI-type starch synthases from rice and
potato, respectively. In addition, the putative N-terminal amino-acid sequence of the encoded protein is identical to that
determined for the N-terminal region of the 75-kDa starch synthase present in the starch granule of hexaploid wheat. Two prominent
starch synthase activities were demonstrated to be present in the soluble fraction of wheat endosperm by activity staining
of the non-denaturing PAGE gels. The most anodal band (wheat SSI) shows the highest staining intensity and results from the
activity of a 75-kDa protein. The wheat SSI mRNA is expressed in the endosperm during the early to mid stages of wheat grain
development but was not detected by Northern blotting in other tissues from the wheat plant. The gene encoding the wheat SSI
(SsI-D1) consists of 15 exons and 14 introns, similar to the structure of the rice starch synthase-I gene. While the exons of wheat
and rice are virtually identical in length, the wheat SsI-D1 gene has longer sequences in introns 1, 2, 4 and 10, and shorter sequences in introns 6, 11 and 14, than the corresponding
rice gene.
Received: 5 June 1998 / Accepted: 29 September 1998 相似文献
3.
Specialisation pattern of the aphid Rhopalosiphum maidis is not modified by experience on a novel host 总被引:1,自引:0,他引:1
Paula P. Caballero Claudio C. Ramírez Hermann M. Niemeyer 《Entomologia Experimentalis et Applicata》2001,100(1):43-52
The effect of alternative host condition on the pattern of specialisation of the aphid Rhopalosiphum maidis (Fitch) was studied. R. maidis commonly occurs in Chile on Johnson grass (Sorghum halepense L.) but rarely on contiguous wheat (Triticum durum L.) crops. The performance of 23 clones of R. maidis on S. halepense (established host) and T. durum (novel host) before and after rearing on wheat for 20 asexual generations was evaluated. Prior and after the period of conditioning on wheat no negative correlation of performance parameters between both hosts was found. Only 8 out of 23 clones survived the 20 asexual generations on wheat. Further, after conditioning on wheat, survival of three out of eight clones increased on wheat and three clones improved r
m on Johnson grass but not on wheat. Although some genotypes of R. maidis were able to withstand wheat stressing conditions, the conditioning on this alternative host did not cause a decreased capacity to use Johnson grass as host. The results suggest that R. maidis performance on wheat is not genetically constrained, and also that conditioning on wheat cannot substantially modify this pattern. 相似文献
4.
Laura‐Jayne Gardiner Piotr Gawroński Lisa Olohan Thorsten Schnurbusch Neil Hall Anthony Hall 《The Plant journal : for cell and molecular biology》2014,80(5):895-904
Mapping‐by‐sequencing analyses have largely required a complete reference sequence and employed whole genome re‐sequencing. In species such as wheat, no finished genome reference sequence is available. Additionally, because of its large genome size (17 Gb), re‐sequencing at sufficient depth of coverage is not practical. Here, we extend the utility of mapping by sequencing, developing a bespoke pipeline and algorithm to map an early‐flowering locus in einkorn wheat (Triticum monococcum L.) that is closely related to the bread wheat genome A progenitor. We have developed a genomic enrichment approach using the gene‐rich regions of hexaploid bread wheat to design a 110‐Mbp NimbleGen SeqCap EZ in solution capture probe set, representing the majority of genes in wheat. Here, we use the capture probe set to enrich and sequence an F2 mapping population of the mutant. The mutant locus was identified in T. monococcum, which lacks a complete genome reference sequence, by mapping the enriched data set onto pseudo‐chromosomes derived from the capture probe target sequence, with a long‐range order of genes based on synteny of wheat with Brachypodium distachyon. Using this approach we are able to map the region and identify a set of deleted genes within the interval. 相似文献
5.
Xingchen Kong Fang Wang Shuaifeng Geng Jiantao Guan Shu Tao Meiling Jia Guoliang Sun Zhenyu Wang Ke Wang Xingguo Ye Jian Ma Dengcai Liu Yuming Wei Youliang Zheng Xiangdong Fu Long Mao Xiujin Lan Aili Li 《Plant biotechnology journal》2022,20(1):75-88
The AGAMOUS-LIKE6 (AGL6)-like genes are ancient MADS-box genes and are functionally studied in a few model plants. The knowledge of these genes in wheat remains limited. Here, by studying a ‘double homoeolog mutant’ of the AGL6 gene in tetraploid wheat, we showed that AGL6 was required for the development of all four whorls of floral organs with dosage-dependent effect on floret fertility. Yeast two-hybrid analyses detected interactions of AGL6 with all classes of MADS-box proteins in the ABCDE model for floral organ development. AGL6 was found to interact with several additional proteins, including the G protein β and γ (DEP1) subunits. Analysis of the DEP1-B mutant showed a significant reduction in spikelet number per spike in tetraploid wheat, while overexpression of AGL6 in common wheat increased the spikelet number per spike and hence the grain number per spike. RNA-seq analysis identified the regulation of several meristem activity genes by AGL6, such as FUL2 and TaMADS55. Our work therefore extensively updated the wheat ABCDE model and proposed an alternative approach to improve wheat grain yield by manipulating the AGL6 gene. 相似文献
6.
Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat 总被引:5,自引:0,他引:5
Anderson GR Papa D Peng J Tahir M Lapitan NL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(7):1297-1303
The Russian wheat aphid is a significant pest problem in wheat and barley in North America. Genetic resistance in wheat is the most effective and economical means to control the damage caused by the aphid. Dn7 is a rye gene located on chromosome 1RS that confers resistance to the Russian wheat aphid. The gene was previously transferred from rye into a wheat background via a 1RS/1BL translocation. This study was conducted to genetically map Dn7 and to characterize the type of resistance the gene confers. The resistant line '94M370' was crossed with a susceptible wheat cultivar that also contains a pair of 1RS/1BL translocation chromosomes. The F2 progeny from this cross segregated for resistance in a ratio of 3 resistant: 1 susceptible, indicating a single dominant gene. One-hundred and eleven RFLP markers previously mapped on wheat chromosomes 1A, 1B and 1D, barley chromosome 1H and rye chromosome 1R, were used to screen the parents for polymorphism. A genetic map containing six markers linked to Dn7, encompassing 28.2 cM, was constructed. The markers flanking Dn7 were Xbcd1434 and XksuD14, which mapped 1.4 cM and 7.4 cM from Dn7, respectively. Dn7 confers antixenosis, and provides a higher level of resistance than that provided by Dn4. The applications of Dn7 and the linked markers in wheat breeding are discussed.Communicated by J. Dvorak 相似文献
7.
8.
The large genome size and the great amount of DNA repeats make it rather diffiult to obtain a representative hexaploid wheat
genomic library. The protocol is given with modifications to phage isolation and to purification of vector and plant DNAs
by electrophoresis in low-concentration SeaKem agarose gels. Representative genomic libraries of the soft wheat variety carrying
a translocated rye chromosome are 1RS were constructed in arecD-minusE. coli strain to reduce recombinational loss.
Erratum: This is a corrected version of an article that appeared in thePlant Molecular Biology Reporter 8(2):85–91.
The online version of the original article can be found at 相似文献
9.
Zengyan Zhang Zhiyong Xin Youzhi Ma Xiao Chen Qiongfang Xu Zhishan Lin 《中国科学C辑(英文版)》1999,42(6):663-668
The wheat line H960642 is a homozygous wheat-Thinopyrum intermedium translocation line with resistance to BYDV by genomicin situ hybridization (GISH) and RFLP analysis. The genomic DNA ofTh. intermedium was used as a probe, and common wheat genomic DNA as a blocking in GISH experiment. The results showed that the chromosome
segments ofTh. intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the translocation line H960642
is a T7DS-7DL-7XL translocation by using 8 probes mapped on the homoeologous group 7 in wheat. The translocation breakpoint
is located between Xpsr680 and Xpsr965 about 90–99 cM from the centromere. The RFLP markers psr680 and psr687 were closely
linked with the BYDV resistance gene. The gene is located on the distal end of 7XL around Xpsr680 and Xpsr687. 相似文献
10.
Conversion of a RAPD-generated PCR product,containing a novel dispersed repetitive element,into a fast and robust assay for the presence of rye chromatin in wheat 总被引:19,自引:0,他引:19
H. A. Francis A. R. Leitch R. M. D. Koebner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,90(5):636-642
Bulk segregant analysis was used to obtain a random amplified polymorphic DNA (RAPD) marker specific for the rye chromosome arm of the 1BL.1RS translocation, which is common in many high-yielding bread wheat varieties. The RAPD-generated band was cloned and end-sequenced to allow the construction of a pair of oligonucleotide primers that PCR-amplify a DNA sequence only in the presence of rye chromatin. The amplified sequence shares a low level of homology to wheat and barley, as judged by the low strength of hybridization of the sequence to restriction digests of genomic DNA. Genetic analysis showed that the amplified sequence was present on every rye chromosome and not restricted to either the proximal or distal part of the 1RS arm. In situ hybridization studies using the amplified product as probe also showed that the sequence was dispersed throughout the rye genome, but that the copy number was greatly reduced, or the sequence was absent at both the centromere and the major sites of heterochromatin (telomere and nucleolar organizing region). The probe, using both Southern blot and in situ hybridization analyses, hybridized at a low level to wheat chromosomes, and no hybridizing restriction fragments could be located to individual wheat chromosomes from the restriction fragment length polymorphism (RFLP) profiles of wheat aneuploids. The disomic addition lines of rye chromosomes to wheat shared a similar RFLP profile to one another. The amplified sequence does not contain the RIS 1 sequence and therefore represents an as yet undescribed dispersed repetitive sequence. The specificity of the amplification primers is such that they will provide a useful tool for the rapid detection of rye chromatin in a wheat background. Additionally, the relatively low level of cross-hybridization to wheat chromatin should allow the sequence to be used to analyse the organization of rye euchromatin in interphase nuclei of wheat lines carrying chromosomes, chromosome segments or whole genomes derived from rye. 相似文献
11.
Li Z Zhou M Zhang Z Ren L Du L Zhang B Xu H Xin Z 《Functional & integrative genomics》2011,11(1):63-70
Fusarium head blight (scab), primarily caused by Fusarium graminearum, is a devastating disease of wheat (Triticum aestivum L.) worldwide. Wheat sharp eyespot, mainly caused by Rhizoctonia cerealis, is one of the major diseases of wheat in China. The defensin RsAFP2, a small cyteine-rich antifungal protein from radish
(Raphanus sativus), was shown to inhibit growth in vitro of agronomically important fungal pathogens, such as F. graminearum and R. cerealis. The RsAFP2 gene was transformed into Chinese wheat variety Yangmai 12 via biolistic bombardment to assess the effectiveness of the defensin
in protecting wheat from the fungal pathogens in multiple locations and years. The genomic PCR and Southern blot analyses
indicated that RsAFP2 was integrated into the genomes of the transgenic wheat lines and heritable. RT-PCR and Western blot proved that the RsAFP2 was expressed in these transgenic wheat lines. Disease tests showed that four RsAFP2 transgenic lines (RA1–RA4) displayed enhanced resistance to F. graminearum compared to the untransformed Yangmai 12 and the null-segregated plants. Assays on Q-RT-PCR and disease severity showed that
the express level of RsAFP2 was associated with the enhanced resistance degree. Two of these transgenic lines (RA1 and RA2) also exhibited enhanced resistance
to R. cerealis. These results indicated that the expression of RsAFP2 conferred increased resistance to F. graminearum and R. cerealis in transgenic wheat. 相似文献
12.
Matsuoka Y Nasuda S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(8):1710-1717
Hexaploid bread wheat was derived from a hybrid cross between a cultivated form of tetraploid Triticum wheat (female progenitor) and a wild diploid species, Aegilops tauschii Coss. (male progenitor). This cross produced a fertile triploid F1 hybrid that set hexaploid seeds. The identity of the female progenitor is unknown, but various cultivated tetraploid Triticum wheats exist today. Genetic and archaeological evidence suggests that durum wheat (T. turgidum ssp. durum) may be the female progenitor. In previous studies, however, F1 hybrids of durum wheat crossed with Ae. tauschii consistently had low levels of fertility. To establish an empirical basis for the theory of durum wheat being the female progenitor of bread wheat, we crossed a durum wheat cultivar that carries a gene for meiotic restitution with a line of Ae. tauschii. F1 hybrids were produced without using embryo rescue techniques. These triploid F1 hybrids were highly fertile and spontaneously set hexaploid F2 seeds at the average selfed seedset rate of 51.5%. To the best of our knowledge, this is the first example of the production of highly fertile F1 hybrids between durum wheat and Ae. tauschii. The F1 and F2 hybrids are both similar morphologically to bread wheat and have vigorous growth habits. Cytological analyses of F1 male gametogenesis showed that meiotic restitution is responsible for the high fertility of the triploid F1 hybrids. The implications of these findings for the origin of bread wheat are discussed. 相似文献
13.
14.
Integration of dinucleotide microsatellites from hexaploid bread wheat into a genetic linkage map of durum wheat 总被引:13,自引:0,他引:13
V. Korzun M. S. Röder K. Wendehake A. Pasqualone C. Lotti M. W. Ganal A. Blanco 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1202-1207
Seventy nine microsatellite markers from hexaploid bread wheat (T. aestivum L.) were integrated into a genetic linkage map of durum wheat (T. turgidum ssp. durum (Desf.) Huns.) created by RFLP segregation data from a population of 65 recombinant inbred lines. The results indicate a
relatively even distribution of microsatellite loci and demonstrate that microsatellite markers from hexaploid wheat provide
an excellent source of molecular markers for use in the genetics and breeding of durum wheat.
Received: 16 July 1998 / Accepted: 13 October 1998 相似文献
15.
Liu W Nie H Wang S Li X He Z Han C Wang J Chen X Li L Yu J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(4):651-657
Wheat yellow mosaic disease, which is caused by wheat yellow mosaic bymovirus (WYMV) and transmitted by soil-borne fungus, results in severe damage on wheat (Triticum aestivum L.) production in China. For development of resistant cultivars to reduce wheat yield losses due to wheat yellow mosaic disease, resistance test and genetic analysis indicated that a single dominant gene in wheat cultivar Yangfu 9311 contributed to the resistance. Bulk segregant analysis was used to identify microsatellite markers linked to the resistance gene in an F2 population derived from the cross Yangfu 9311 (resistant) × Yangmai 10 (susceptible). Microsatellite markers Xwmc41, Xwmc181, Xpsp3039, and Xgwm349 were co-dominantly or dominantly linked with the gene responsible for WYMV resistance at a distance of 8.1–11.6 cM. Based on the wheat microsatellite consensus map and the results from amplification of the cultivar Chinese Spring nulli-tetrasomic stocks, the resistance gene to wheat yellow mosaic disease derived from Yangfu 9311, temporarily named as YmYF, was thus mapped on the long arm of chromosome 2D (2DL). 相似文献
16.
Fanguo Chen Feng Zhao Shuwei Liu Guangmin Xia 《Molecular breeding : new strategies in plant improvement》2009,24(2):117-126
A PCR-based strategy was applied to obtain the DNA sequence of γ-gliadin open reading frames present in line II-12, a derivative
from a somatic hybrid between bread wheat (Triticum aestivum L.) cv. Jinan177 and tall wheatgrass (Lophopyrum ponticum, 10×). A total 50 analysable sequences were obtained, 18 from II-12 and 16 each from the parents. Amplicon length ranged
from 720 to 936 bp, corresponding to a putative mature protein of 239–309 residues. The primary structure of these putative
proteins comprised five domains, of which only two varied in length. Phylogenetic analyses showed that the mature γ-gliadin
sequences fell into four major clades. Group 1 contained sequences shared between II-12 and L. ponticum, suggesting that some L. ponticum γ-gliadin genes are present in the introgression line. Group 3 has five Jinan177 and five II-12 sequences, indicating that
II-12 also carries wheat versions of Gli-1. Group 2 and 4 comprised four and two II-12, three and one Jinan177 as well as one and four L. ponticum sequences, respectively. Fewer genes encoding coeliac disease epitopes were present in II-12 than in the wheat donor parent.
Three II-12 γ-gliadins and one from the wheat parent contained an odd number of cysteine residues, and two of them had an
additional cysteine residue at the amino end of domain V. The possible use of II-2 for improving quality of bread wheat is
discussed. 相似文献
17.
T. E. Miller J. Hutchinson V. Chapman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,61(1):27-33
Summary An attempt to produce a set of addition lines of Aegilops sharonensis to the wheat variety Chinese Spring produced only one addition line. This was due to preferential transmission of one chromosome from Ae. sharonensis. This chromosome was studied in detail by established cytological methods of chromosome observation and by the newer techniques of C-banding and in situ hybridization of a cloned DNA sequence. The chromosome was found to be partially homologous to an Ae. sharonensis chromosome of similar behaviour in another wheat addition line. The incomplete homology of the two Ae. sharonensis chromosomes was due to the presence of a translocated segment of a wheat chromosome. — Substitution lines of the Ae. sharonensis chromosome for wheat homoeologous group 4 were produced and the Ae. sharonensis chromosome thereby designated 4 S
l
. 相似文献
18.
R. M. D. Koebner 《Biochemical genetics》1987,25(7-8):591-602
The aneuploids of Chinese Spring wheat have been used to locate the genes(Ti-2) coding for a novel series of trypsin inhibitors to the long arms of the homoeologous group 5 chromosomes. Three allelic variants at the 5D locus were detected in a limited survey among wheat varieties, but no variation at the loci on either chromosome 5A or chromosome 5B was detected. Homoeoloci were found in a number of alien relatives, and in the majority of cases, these were present on the group 5 homoeologue. However, inAegilops umbellulata, theTi-U2 locus was located on a chromosome presumed to belong to homoeologous group 1. NoHordeum vulgare orH. chilense Ti-2 gene was expressed in a wheat background. This new marker will be especially useful as a screening mechanism for nullisomy of chromosome 5B in work aimed at introgression of alien chromatin into wheat.The Agricultural Genetics Company is thanked for financial support. 相似文献
19.
Annemarie S. Buchel Richard Molenkamp John F. Bol Huub J. M. Linthorst 《Plant molecular biology》1996,30(3):493-504
A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family. 相似文献
20.
Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat 总被引:2,自引:0,他引:2
Xunfen Chen Justin D. Faris Jinguo Hu Robert W. Stack Tika Adhikari Elias M. Elias Shahryar F. Kianian Xiwen Cai 《Molecular breeding : new strategies in plant improvement》2007,19(2):113-124
Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified
in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T.
dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region
amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular
marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance
of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to
3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning
of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection. 相似文献