U1 snRNP increases RNA Pol II elongation rate to enable synthesis of long genes

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Allosteric transcription stimulation by RNA polymerase II super elongation complex

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MDC1 maintains active elongation complexes of RNA polymerase II

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POINT technology illuminates the processing of polymerase-associated intact nascent transcripts

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Efficient RNA polymerase II pause release requires U2 snRNP function

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Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription

The cylindrical shape of the fission yeast cell is generated by linear polarized growth from its cell ends. Using immunofluorescence and live imaging microscopy, we have investigated the roles of the cell end marker tea1p in generating linear polarized growth. We found that tea1p is primarily transported on plus ends of microtubules from the vicinity of the nucleus to the cell ends, and that its movement near the nucleus is independent of the kinesin tea2p. Deletion analysis identified a coiled-coil domain in tea1p essential for its retention at cell ends, and demonstrated that tea1p exerts different functions dependent on its location. On the tips of microtubules, tea1p prevents the curling of microtubules around the cell ends, whereas it is required for maintaining linear cell growth and for retention of polarity factors such as the Dyrk kinase pom1p, the CLIP170-like tip1p, and tea2p at the cell ends. We propose that tea1p has roles in organizing the microtubule cytoskeleton on the tips of microtubules, and in the retention of factors at the cell ends necessary for the cell to grow in a straight line.     

SPT5 stabilizes RNA polymerase II,orchestrates transcription cycles,and maintains the enhancer landscape

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A BRD4-mediated elongation control point primes transcribing RNA polymerase II for 3′-processing and termination

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基于PacBio测序数据的蜜蜂球囊菌转录因子、融合基因及RNA编辑事件的鉴定

【目的】本研究旨在利用已获得的PacBio单分子实时(single molecule real-time, SMRT)测序数据对蜜蜂球囊菌Ascosphaera apis菌丝(AaM)和孢子(AaS)中的转录因子(TF)、融合基因和RNA编辑事件进行鉴定和分析,以期丰富蜜蜂球囊菌的相关信息,并为进一步探究它们的功能提供理论依据。【方法】利用BLASTx工具将AaM和AaS的全长转录本序列比对到Nr, Swiss-Prot和KEGG数据库以获得一致性最高的蛋白序列,再利用hmmscan软件将上述蛋白序列比对到Plant TFdb数据库以获得TF的分类及注释信息。采用TOFU软件中的fusion_finder.py程序进行融合基因的预测,进而分析融合基因的序列和位置信息。使用SAMtools预测AaM和AaS中的RNA编辑事件,再利用ANNOVAR软件对RNA编辑事件进行注释,进而采用相关生物信息学软件对RNA编辑位点基因进行GO功能和KEGG通路注释。【结果】在AaS中共鉴定到17个TF家族的213个TF,其中C2H2家族包含的TF成员最多。在AaM和AaS中分别鉴定到921和510个融合基因,二者共有的融合基因为510个,特有的融合基因分别为411和0个。在AaM和AaS中分别鉴定到547和191次RNA编辑事件,其中AaM中同义单核苷酸突变的数量最多,AaS中非同义单核苷酸突变的数量最多。此外,在AaM中鉴定到12种碱基替换类型,其中发生C->T的RNA编辑事件数量最多,达到158次;在AaS中鉴定到9种碱基替换类型,其中发生C->T和G->T的RNA编辑事件数量最多,均有42次。AaM和AaS中RNA编辑位点基因分别涉及19和24个GO功能条目;此外还能注释到11和20条KEGG通路。【结论】蜜蜂球囊菌的菌丝和孢子中含有丰富的TF、融合基因和RNA编辑位点;转录因子C2H2家族与蜜蜂球囊菌菌丝和孢子的生长发育和细胞活动具有潜在关联;RNA编辑事件的碱基替换类型在蜜蜂球囊菌和其他物种中具有物种特异性;RNA编辑可能在蜜蜂球囊菌菌丝和孢子的生长和代谢中发挥作用。