SPT5 stabilizes RNA polymerase II,orchestrates transcription cycles,and maintains the enhancer landscape

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Stress-induced transcriptional memory accelerates promoter-proximal pause release and decelerates termination over mitotic divisions

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The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity

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High-throughput functional dissection of noncoding SNPs with biased allelic enhancer activity for insulin resistance-relevant phenotypes

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A new deep learning technique reveals the exclusive functional contributions of individual cancer mutations

Cancers are caused by genomic alterations that may be inherited, induced by environmental carcinogens, or caused due to random replication errors. Postinduction of carcinogenicity, mutations further propagate and drastically alter the cancer genomes. Although a subset of driver mutations has been identified and characterized to date, most cancer-related somatic mutations are indistinguishable from germline variants or other noncancerous somatic mutations. Thus, such overlap impedes appreciation of many deleterious but previously uncharacterized somatic mutations. The major bottleneck arises due to patient-to-patient variability in mutational profiles, making it difficult to associate specific mutations with a given disease outcome. Here, we describe a newly developed technique Continuous Representation of Codon Switches (CRCS), a deep learning-based method that allows us to generate numerical vector representations of mutations, thereby enabling numerous machine learning-based tasks. We demonstrate three major applications of CRCS; first, we show how CRCS can help detect cancer-related somatic mutations in the absence of matched normal samples, which has applications in cell-free DNA–based assessment of tumor mutation burden. Second, the proposed approach also enables identification and exploration of driver genes; our analyses implicate DMD, RSK4, OFD1, WDR44, and AFF2 as potential cancer drivers. Finally, we used CRCS to score individual mutations in a tumor sample, which was found to be predictive of patient survival in bladder urothelial carcinoma, hepatocellular carcinoma, and lung adenocarcinoma. Taken together, we propose CRCS as a valuable computational tool for analysis of the functional significance of individual cancer mutations.     

INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II

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Structure of a backtracked hexasomal intermediate of nucleosome transcription

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Dynamic chromatin state profiling reveals regulatory roles of auxin and cytokinin in shoot regeneration

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The miR-430 locus with extreme promoter density forms a transcription body during the minor wave of zygotic genome activation

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Two distinct mechanisms of RNA polymerase II elongation stimulation in vivo

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