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对采自我国不同地区的菌寄生属标本进行分类研究,发现了该属的3个新种。鹅膏菌寄生Hypomyces amaniticola生长于鹅膏属真菌的子实体上,子囊壳埋生或半埋生于菌丝层中,橘黄色至黄褐色,卵圆形至梨形;子囊圆柱形,具8个孢子;子囊孢子长椭圆形至椭圆形,两端钝圆,无分隔,表面光滑。拟完整菌寄生H.completiopsis以牛肝菌为寄主,子囊壳橘黄色至黑褐色,梨形至近球形;子囊圆柱形,具8个孢子;子囊孢子纺锤形至披针形,两端具细尖,表面具疣。云南菌寄生H.yunnanensis子囊壳半埋生或近表生,黄褐色至褐色,梨形至近球形;子囊孢子近纺锤形,两端具细尖,1个分隔,表面具疣。提供了新种的详细宏观和微观特征描述及图示。此外,发现该属的2个中国新记录种,即小孢菌寄生H.microspermus和盘菌菌寄生H.stephanomatis,对我国材料的形态特征与原始描述进行了比较。 相似文献
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本文报道虫囊菌属(Laboulbenia)2个新种,中国新记录种及新记录变种各1个:海南虫囊菌(L.hainanensis Ye et Y.H.Shen sp.nov.)寄生于齿负泥虫[Lema eoromandeliana (Fabricius)],福建虫囊菌(L.fujianensis Ye sp.nov.)寄生于长唇步甲属(Dolichoctissp.),蠕形虫囊菌(L.vermiiormis Balazuc)寄生于锯缘步甲(Peripristus alter Cast.),瘤壳虫囊菌婆罗洲变种(L.thyreopteri Thaxt.var.borneensis Thaxt.)寄生于四斑长唇步甲[Dolichoctis tetraspilotus(Macheay)]。 本文所研究的全部标本都保存于广东省微生物研究所。 相似文献
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江苏省云台山白粉菌研究Ⅴ.白粉菌的一新变种和一新记录种 总被引:1,自引:0,他引:1
报道了白粉菌目的一个新变种和一个中国新记录种.新变种:苋生蒙加拉白粉菌Erysiphemunjalii var.amaranthicola,寄生在苋科皱果苋Amaranthus viridis上;新记录种:山田叉丝壳Microsphaera yamadai,寄生在鼠李科拐枣Hovenia dulcis上.新变种有中文和拉丁文描述.研究标本保存在中国科学院微生物研究所菌物标本馆(HMAS). 相似文献
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本文报道虫囊菌属2个新种,中国新记录种及新记录变种各1个:海南虫囊菌寄生于齿负泥虫,福建虫囊菌寄生于长唇步甲属蠕形虫囊菌寄生于锯缘步甲瘤壳虫囊菌婆罗洲变种寄生于四斑长唇步甲。 相似文献
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有关马鞍菌属 Helvella 的历史,属种概念以及中国已知种的检索表已作过报道(刘波等,1985)。本文描述了近年来采到的两个新种:粘马鞍菌 Helvella glutinosa sp.nov.和伞形马鞍菌 H.galeriformis sp.nov.;四个新记录:白柄马鞍菌 H.albellu Quél.,小马鞍菌H.helvellula(Dur.et Mont.)Dissing,乳白马鞍菌 H.lactea Boud.和脉马鞍菌 H.phlebo-phora Pat.et Doass.。此外,斑点马鞍菌 H.maculata Weber 虽已作过报道和描述(李静丽等,1986),然而由于它是一个非常具特征性的种,而且有一限制的地理分布,因此本文也对它进行了更充分的描述和讨论。 相似文献
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前文分别报道了长叶车前花叶病毒上海分离侏(RMVsh)单克隆抗体的制备及根据它们在不同免疫反应中的特性,将它们分为两组,分别识别性质不同的抗原决定簇。本文采用修改的Friguet方法测定了各组内各单克隆抗体之间的增值反应(Additivity Reaction)特性,并分析了它们识别抗原决定簇的特性。村料与方法一、病毒及单克隆抗体长叶车前花叶病毒上海分离株及其单克隆抗体1H2、7H1、10H1、11H2、12H3、17H6、29H1来源、制备和特性见前文报道。二、抗原饱和曲线的测定抗原饱和曲线测定采用间接ELISA办法,抗原浓度为2μg/ml。 相似文献
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The availability of tyrosyl residues to surface iodination was analyzed for histone 2A (H2A), histone 2B (H2B), and the H2A/H2B complex. When H2A is free in solution (200 mM NaCl, pH 7.4) tyrosine-39 and one or both tyrosines-50 and -57 were readily iodinated. Tyrosines-83 and -121 of H2B were iodinated, both when the histone was free in solution and when it was associated with H2A, while tyrosines-37, -40, and -42 of H2B were not iodinated under either condition. When H2A and H2B were associated or covalently cross-linked, all tyrosyl residues of H2A were unavailable for iodination. We also found that the iodination of nondenatured H2A and H2B did not inhibit formation of the H2A/H2B complex. These results indicate that the amino-terminal regions of the hydrophobic portions of H2A and H2B undergo significant conformational changes upon formation of the H2A/H2B complex. These conformational shifts occur in the same region of the H2A/H2B complex that contains a contact site between H2A and H2B in the nucleosome, thus indicating an involvement of this region in chromatin assembly. 相似文献
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Separation of rat tissue histone H1 subtypes by reverse-phase h.p.l.c. Identification and assignment to a standard H1 nomenclature. 总被引:1,自引:0,他引:1
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H1 histones from rat liver and rat testis were separated by reverse-phase h.p.l.c. Within 40 min six subfractions (H1(0), H1b, H1a, H1d, H1e + H1c and H1c) and seven subfractions (H1(0), H1b, H1a, H1d, H1e + H1c, H1c and H1t) respectively were isolated by using a linear acetonitrile gradient. Each individual H1 subtype was identified either by comparing the H1 variants (contained in both tissues but in different quantities) or by SDS/PAGE and acetic acid/urea/PAGE. Moreover, all H1 variants were characterized by amino acid analyses. The amino acid compositions of rat histone subfractions H1(0), H1b and H1e were determined for the first time. It was possible to classify unambiguously the H1 subfractions obtained by h.p.l.c. by following the standardized H1 nomenclature for electrophoretic systems recommended by Lennox, Oshima & Cohen [(1982) J. Biol. Chem. 257, 5183-5189]. Incorrect assignments that have been made in various publications are discussed. 相似文献
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Tanaka Y Tawaramoto-Sasanuma M Kawaguchi S Ohta T Yoda K Kurumizaka H Yokoyama S 《Methods (San Diego, Calif.)》2004,33(1):3-11
Nucleosomes reconstituted from bacterially expressed histones are useful for functional and structural analyses of histone variants, histone mutants, and histone post-translational modifications. In the present study, we developed a new method for the expression and purification of recombinant human histones. The human histone H2A, H2B, and H3 genes were expressed well in Escherichia coli cells, but the human histone H4 gene was poorly expressed. Therefore, we designed a new histone H4 gene with codons optimized for the E. coli expression system and constructed the H4 gene by chemically synthesized oligodeoxyribonucleotides. The recombinant human histones were expressed as hexahistidine-tagged proteins and were purified by one-step chromatography with nickel-nitrilotriacetic acid agarose in the presence of 6 M urea. The H2A/H2B dimer and the H3/H4 tetramer were refolded by dialysis against buffer without urea, and the hexahistidine-tags of the histones in the H2A/H2B dimer and the H3/H4 tetramer were removed by thrombin protease digestion. The H2A/H2B dimer and the H3/H4 tetramer obtained by this method were confirmed to be proficient in nucleosome formation by the salt dialysis method. The human CENP-A gene, the centromere-specific histone H3 variant, contains 28 minor codons for E. coli. A new CENP-A gene optimized for the E. coli expression system was also constructed, and we found that the purified recombinant CENP-A protein formed a nucleosome-like structure with histones H2A, H2B, and H4. 相似文献
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Hexaploid H1 (ES) cells established from octaploid H1 cells are as DNA stable as pentaploid H1 cells
Hexaploid H1 (ES) cells (6H1 cells) were established from octaploid H1 cells (8H1 cells), as were pentaploid H1 cells (5H1 cells). 6H1 cells were compared with 5H1 cells. The number of chromosomes of 6H1 cells was 115, 20 more than the 95 of 5H1 cells. The durations of G(1), S, and G(2)/M phases of 6H1 cells were 3, 7, and 6 h, respectively, almost the same as those of 5H1 cells. The cell volume of 6H1 cells was equivalent that of 5H1 cells. The morphology of 6H1 cells was flattened circular cluster, different from the spherical cluster of 5H1 cells. 6H1 cells exhibited alkaline phosphatase activity as well as 5H1 cells. The DNA content of 6H1 cells was stable and maintained for 300 days of culturing, the same as that of 5H1 cells. The DNA stability of 6H1 cells was explained using a hypothesis concerning the DNA structure of polyploid cells because the asymmetric configuration of homologous chromosomes in 6H1 cells inhibited chromosome loss. 相似文献
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Two lines of dodecaploid H1 embryonic stem cells, 12H1 and 12H1(?) cells (mouse-originated cells), were established through polyploidization of two hexaploid H1 cells, 6H1 and 6H1(?) cells, which were cultured in L15F10 (7:3) medium with and without leukemia inhibitory factor (LIF), respectively. The G1, S, and G2/M phase fractions of 12H1 and 12H1(?) cells were almost the same as those of 6H1 and 6H1(?) cells, respectively, but the doubling time of cell proliferation was prolonged, suggesting that cell death occurred in 12H1 and 12H1(?)cells. The cell volumes of 12H1 and 12H1(?) cells were about double those of 6H1 and 6H1(?) cells, respectively. 12H1 and 12H1(?) cells showed near-negative activity of alkaline phosphatase and no ability to form teratocarcinomas in mouse abdomen, suggesting that 12H1 and 12H1(?) cells lost pluripotency. The DNA contents of 12H1 and 12H1(?) cells decayed in long-term culturing, suggesting that 12H1 and 12H1(?) cells were DNA-unstable. Possible explanations for the lost pluripotency and for the DNA decay in 12H1 and 12H1(?) cells are presented. 相似文献
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Kohzaburo Fujikawa‐Yamamoto Xianwen Luo Minoru Miyagoshi Hiroko Yamagishi 《Journal of cellular physiology》2010,223(2):369-375
Pentaploid H1 (ES) cells (5H1 cells) were accidentally obtained through one‐cell cloning of octaploid H1 (ES) cells (8H1 cells) that were established from tetraploid H1 (ES) cells (4H1 cells) polyploidized using demecolcine. The number of chromosomes of 5H1 cells was 100, unlike the 40 of diploid H1 (ES) cells (2H1 cells), 80 of 4H1, and 160 of 8H1 cells. The durations of G1, S, and G2/M phases of 5H1 cells were 3, 7, and 6 h, respectively, almost the same as those of 2H1, 4H1, and 8H1 cells. The cell volume of 5H1 cells was half of that of 8H1 cells, suggesting that 5H1 cells were created through abnormal cell divisions of 8H1 cells. The morphology of growing 5H1 cells was a spherical cluster similar to that of 2H1 cells and differing from the flagstone‐like shape of 4H1 and 8H1 cells. Pentaploid solid tumors were formed from 5H1 cells after interperitoneal injection into the mouse abdomen, and they contained endodermal, mesodermal, and ectodermal cells as well as undifferentiated cells, suggesting both that the DNA content of 5H1 cells was retained during tumor formation and that the 5H1 cells were pluripotent. The DNA content of 5H1 cells was stable in long‐term culturing as 2H1 cells, meaning that 5H1 and 2H1 cells shared similarities in DNA structure. The excellent stability of the DNA content of 5H1 cells was explained using a hypothesis for the DNA structure of polyploid cells because the pairing of homologous chromosomes in 5H1 cells is spatially forbidden. J. Cell. Physiol. 223: 369–375, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
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土壤来源的五个苏云金芽孢杆菌新亚种的鉴定 总被引:4,自引:0,他引:4
从中国土壤中分离的大量苏云金芽孢杆菌菌株中鉴定出H42、H43、H56、H60及H62等5种新H血清型,并进行了形态、培养特征、生化反应、晶体蛋白质成分及毒力特性等项检测鉴定,鉴定了5个苏云金芽孢杆菌新亚种:
Bacillus thuringiensis subsp. jinghongiensis (H42), B.thuringiensis subsp. guiyangiensis (H43),B.thuringiensis subsp. rongseni(H56),B.thuringiensis subsp. pingluonsis(H60)及B.thuringiensis subsp. zhaodongensis(H62)
。毒力生物测定证明5个新亚种的代表菌株对棉铃虫(Heliothis armigera)\,小菜蛾(Plutella xylostella)\,柳蓝叶甲(Plagiodera versicolora)幼虫均无毒力。H42、H43、H56、H60对埃及伊蚊(Aedes aegypti)\,斑须按蚊(Anopheles stephensi)及尖音库蚊(Culex pipiens)亦均无毒;H62对埃及伊蚊无毒,但对尖音库蚊与斑须按蚊有低毒。 相似文献
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GREGORY A. AKPAN M. GUL HOSSAIN 《Botanical journal of the Linnean Society. Linnean Society of London》1998,126(3):207-216
Studies were made of the morphology and karyotype of accessions of H. asper, H. cannabinus and H. surattensis. There were six simple epicalyx segments in H. asper , nine in H. cannabinus and ten branched and leafy epicalyx segments in H. surattensis. Karyotypic analysis of the species showed a preponderance of median centromeres. H. surattensis had a more symmetrical karyotype and smaller chromosomes than H. asper and H. cannabinus. H. asper had the longest chromosomes. Ten of their chromosomes were significantly different in length, while four were significantly different in arm ratio. 相似文献