华南吴萸的化学成分及抗炎活性研究CSCD

综合利用各种分离提取技术对芸香科植物华南吴萸的化学成分进行研究,并利用核磁共振波谱技术进行结构鉴定。共分离纯化鉴定出13个化合物,分别为柠檬苦素(1)、6α-乙酰氧基-12α-羟基吴茱萸内酯醇(2)、12α-hydroxyevodol(3)、12α-羟基柠檬苦素(4)、臭辣树交酯A(5)、β-别隐品碱(6)、瑞特西宁(7)、野花椒甲素(8)、bis(2-ethylhexyl)benzene-1,2-dicarboxylate(9)、bejolghotin G(10)、lynoiresinol(11)、(-)-(7R,7′R,7′′S,8S,8′S,8′′S)-4′,4′′-dihydroxy-3,3′,3′′,5-tetramethoxy-7,9′:7′,9-diepoxy-4,8′′-oxy-8,8′-sesquineolignan-7′′,9′′-diol(12)、3′,6-dipropyl apigenin(13)。其中化合物6、8、9和12为首次从吴茱萸属中分离得到,其余化合物均为首次从华南吴萸中分离得到。并对其化合物进行乳酸脱氢酶(lactate dehydrogenase,LDH)释放的抑制率测试,结果表明,化合物13对LDH释放的抑制活性较好,证明该化合物具有一定的抗炎活性。     

芒果叶化学成分研究Ⅲ

为了解芒果(Mangifera indica L.)的化学成分,从芒果叶70%乙醇提取物中分离鉴定了8个单体化合物,经波谱分析,分别鉴定为:5-(β-D-glucopyranosyloxy)-2-hydroxy benzoic acid methyl ester(1)、methyl salicylate glucoside(2)、对羟基苯甲酸(3)、nikoenoside(4)、(6R,9R)-3-oxo-α-ionol-β-D-glucopyranoside(5)、byzantionoside B(6)、icariside B2(7)和2-ethyl-3-methylmaleimide N-β-D-glucopyranoside(8)。化合物1、2、4~7均为首次从芒果属中分离得到,化合物8首次从该种中分离得到,化合物1的NMR数据是首次报道。     

异叶三宝木叶的化学成分研究(英文)

从异叶三宝木(Trigonostemon flavidus)叶中分离得到9个化合物,经波谱数据分析鉴定为robustic acid(1),1-[6-hydroxy-2-methoxy-2″,2″-dimethylpyrano-(5″,6″:3,4)]-2-(4’-methoxyphenyl)-1,2-ethanedione(2),3β-ursolic acid(3),(6S,7E)-6-hydroxy-4,7-megastigmadien-3,9-dione(4),3(-hydroxy-5,6-epoxy-7-megastigmen-9-one(5),(3R,6R,7E)-3-hydroxy-4,7-megastigmadien-9-one(6),loliolide(7),methyl P-coumarate(8),和methyl si-napate(9)。化合物1～7和9为首次从三宝木属(Trigonostemon)植物中分离得到。     

苦玄参的化学成分研究

对广西传统的抗菌消炎药用植物苦玄参进行了化学成分研究,采用柱色谱进行分离纯化,运用波谱法进行了结构解析,共鉴定得到7个化合物。它们分别为:芹菜素(1)、芹菜素-7-O-β-D-葡萄糖酸(2)、芹菜素-7-O-α-L-吡喃鼠李糖基(1→2)-β-D-吡喃葡萄糖酸(3)、迷迭香酸(4)、苦玄参苷Ⅳ(5)、苦玄参苷Ⅹ(6)、阿克替苷(7)。其中化合物2、3、4为首次从该植物中分离得到。     

苦槛蓝叶的化学成分及其抑菌活性研究

为研究苦槛蓝(Myoporum bontioides A.Gray)的化学成分,采用多种柱色谱技术从苦槛蓝叶中分离得到13个化合物,它们的结构分别鉴定为:野黑樱苷(1)、类叶升麻苷(2)、5,7-二羟基二氢黄酮(3)、3-O-β谷甾醇苷(4)、(3R)-oct-1-en-3-ol-O-β-D-glucopyranosyl-(1″→2′)-O-β-D-glucopyranoside(5)、7-甲氧基香橙素(6)、异樱花素(7)、匙叶桉油烯醇(8)、愈创木醇(9)、(1S,2R,5S,6R)-2,6-bis(5-methoxy-3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(10)、(1R,2S,5R,6S)-2-(4-hydroxy-3-methoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(11)、去甲基川陈皮素(12)和3′,4′,5,6,7,8-六甲氧基黄酮(13),其中化合物4、5、7~11为首次从苦槛蓝植物中分离得到。刃天青显色法测试部分化合物对大肠杆菌与金黄色葡萄球菌的抑制活性,结果表明,5,7-二羟基二氢黄酮(3)对大肠杆菌与金黄色葡萄球菌有一定的抑制作用,其MIC值为62.50μg mL–1。     

竹叶榕根的化学成分研究

从竹叶榕(Ficus stenophyllaHemsl.)根乙醇提取物中分离得到10个化合物,经理化常数和波谱数据分析,分别鉴定为3,4-二氢补骨酯素(3,4-dihydropsoralen,1)、7-羟基香豆素(7-hydroxycoumarin,2)、香柠檬内酯(bergapten,3)、补骨酯素(psoralen,4)、( )-儿茶素(catechin,5)、芹菜素(apigenin,6)、蔗糖(sucrose,7)、香草酸(vanillic acid,8)、胡萝卜苷(daucosterol,9)和豆甾醇(stigmasterol,10)。化合物1~10均为首次从该植物中分离得到,其中化合物1为新天然产物。     

旋覆花的化学成分研究(英文)

通过应用硅胶柱色谱,Sephadex LH-20柱色谱,以及高效液相制备色谱等手段,从旋覆花地上部分分离纯化得到10个化合物,再通过光谱学方法,并结合化合物的理化性质鉴定为泽兰内酯(1)、7-羟基香豆素(2)、瑞香素(3)、东莨菪素(4)、蟛蜞菊内酯(5)、枸橼苦素(6)、杜仲树脂酚(7)、丁香酸(8)、香草酸(9)和异丁香酸(10)。化合物1～10均为首次从旋覆花属植物中分离得到。     

大叶山楝根的化学成分及细胞毒活性研究

该研究从大叶山楝根95%乙醇提取物的乙酸乙酯萃取物中分离得到10个化合物,经波谱解析分别鉴定为schleicheol 2(1),β-扶桑甾醇(2),fregenedadiol(3),异巴西红厚壳素(4),丁香酯素(5),表丁香酯素(6),graminone A(7),sylvatesmin(8),Z-6-十九烯酸甲酯(9),棕榈酸(10)。其中,化合物1-4、化合物7-9为首次从山楝属植物中分离得到。细胞毒活性测试结果表明,化合物1和化合物2对人胃癌细胞SGC-7901生长具有一定的抑制活性。     

雪白睡莲花酚类成分研究

采用大孔树脂、硅胶、ODS RP-18和Sephadex LH-20柱从雪白睡莲的干燥花蕾中分离得到7个酚类成分,通过理化性质和波谱学方法鉴定化合物的结构,分别为:isostrictiniin(1)、老鹤草素(2)、鞣花酸(3)、短叶苏木酚(4)、Annulatin 3’-O-β-D-xyloside(5)、短叶苏木酚酸甲酯(6),1,2,3,4,6-五没食子酰基葡萄糖(7)。这些化合物均为首次从该植物中分离得到,化合物1,2,5和6首次从该属植物中分得。     

柠檬苦素类化合物抗病原体作用及机制研究进展

柠檬苦素类化合物是高度氧化的三萜类化合物,对动植物病原体表现出抑制作用,具有一定的抗菌活性,将其作为一种天然的抗病原体物质具有很好的研究价值和应用潜能。本文将综述柠檬苦素类物质对病原体的影响、作用机制以及柠檬苦素类物质的吸收代谢情况,以期为柠檬苦素类化合物应用于食品、医药等行业提供基础理论参考。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.