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1.
Sung-Heun Cho Kang Seon Lee Dong-Sung Shin Ji-Hye Han Ki Seok Park Cheon Hee Lee Kwan Ha Park Seung Bum Kim 《Systematic and applied microbiology》2010
The taxonomic positions of five Gram-negative, non-spore-forming and non-motile bacterial strains isolated from the rhizosphere of sand dune plants were examined using a polyphasic approach. The analysis of the 16S rRNA gene sequence indicated that all of the isolates fell into four distinct phylogenetic clusters belonging to the genus Chryseobacterium of the family Flavobacteriaceae. The 16S rRNA gene sequence similarities of isolates to mostly related type strains of Chryseobacterium ranged from 97.5% to 98.5%. All strains contained MK-6 as the predominant menaquinone, and iso-C15:0, iso-C17:0 3-OH and a summed feature of iso-C15:0 2-OH and/or C16:1 ω7c as the dominant fatty acids. Combined phenotypic, genotypic and chemotaxonomic data supported that they represented four novel species in the genus Chryseobacterium, for which the names Chryseobacterium hagamense sp. nov. (type strain RHA2-9T=KCTC 22545T=NBRC 105253T), Chryseobacterium elymi sp. nov. (type strain RHA3-1T=KCTC 22547T=NBRC 105251T), Chryseobacterium lathyri sp. nov. (type strain RBA2-6T=KCTC 22544T=NBRC 105250T), and Chryseobacterium rhizosphaerae sp. nov. (type strain RSB3-1T=KCTC 22548T=NBRC 105248T) are proposed. 相似文献
2.
Olga I. Nedashkovskaya Larissa A. Balabanova Natalia V. Zhukova So-Jeong Kim Irina Y. Bakunina Sung-Keun Rhee 《Archives of microbiology》2014,196(10):745-752
A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130T, that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618T and Flavobacterium ponti CCUG 58402T, and 95.3–92.5 % to other recognized Flavobacterium species. The prevalent fatty acids of strain 10Alg 130T were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids. The DNA G+C content of the type strain was 34.3 mol%. The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp. nov. is proposed. The type strain is 10Alg 130T (=KCTC 32467T = KMM 6686T). 相似文献
3.
Debananda S. Ningthoujam Salam Nimaichand Dollyca Ningombam K. Tamreihao Li Li Yong-Guang Zhang Juan Cheng Min-Jiao Liu Wen-Jun Li 《Antonie van Leeuwenhoek》2013,104(6):1135-1141
A strain of Streptomyces, MBRL 179T, isolated from a sample from a Limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain formed a monophyletic clade with Streptomyces spinoverrucosus NBRC 14228T (16S rRNA gene sequence similarity of 99.3 %) in the Neighbour-joining tree. DNA–DNA hybridization experiment gave a DNA–DNA relatedness value of 34.7 % between MBRL 179T and S. spinoverrucosus NBRC 14228T. Strain MBRL 179T contained LL-diaminopimelic acid, xylose, glucose, and mannose in the whole cell-wall hydrolysates along with small amount of ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, with other unknown phospholipids and aminophospholipid. MK-9(H6), MK-9(H8) and MK-9(H4) were the predominant menaquinones detected. The major fatty acids were anteiso-C16:0 (28.1 %), iso-C16:0 (20.3 %), C16:0 (9.4 %) and anteiso-C17:0 (8.3 %). The G+C content of the genomic DNA was 71.1 %. Based on the polyphasic experiment results, the strain MBRL 179T merits recognition as a representative of a novel species of the genus Streptomyces for which the name Streptomyces muensis sp. nov. is proposed; the type strain is MBRL 179T (=JCM 17576T = KCTC 29124T). 相似文献
4.
Wen-Jun Li Salam Nimaichand Zhao Jiang Min-Jiao Liu Thi-Nhan Khieu Chang-Jin Kim Wael N. Hozzein Dong-Jin Park Mohammed A. M. Wadaan Debananda S. Ningthoujam 《Antonie van Leeuwenhoek》2014,106(6):1119-1126
Hundung Limestone habitat, Manipur, India is an unexplored site for microbial diversity studies. Using polyphasic taxonomy, a Streptomyces strain, MBRL 172T, has been characterized. The strain was found to show highest 16S rRNA gene sequence similarity with Streptomyces coeruleofuscus NBRC 12757T (99.2 %). The DNA relatedness between MBRL 172T and S. coeruleofuscus NBRC 12757T, and between MBRL 172T and Streptomyces nogalater NBRC 13445T, were 36.8 ± 4.4 and 52.5 ± 2.7 %, respectively. Strain MBRL 172T was found to contain ll-diaminopimelic acid as the diagnostic diamino acid and glucose, mannose and xylose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones detected were MK-9(H6) and MK-9(H8). The cellular fatty acids identified were mainly saturated fatty acids: anteiso-C15:0, iso-C16:0 and iso-C15:0. Based on differences in the biochemical and molecular characteristics from its closest relatives, the strain can be proposed to represent a novel taxon in the genus Streptomyces, for which the name Streptomyces canchipurensis is proposed, with the type strain MBRL 172T (=JCM 17575T = KCTC 29105T). 相似文献
5.
Lei Zhang Linfang Wei Lingfang Zhu Changfu Li Yao Wang Xihui Shen 《Antonie van Leeuwenhoek》2014,105(4):653-661
A Gram stain-negative, strictly aerobic, rod-shaped, non-motile and deep-yellow-coloured bacterial strain, designated ZFJR-3T, was isolated from the stem of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and characterized by using a polyphasic approach. The novel isolate grew optimally at 25–28 °C and in the absence of NaCl. Flexirubin-type pigments were produced. The predominant respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids were iso-C15:0 (29.2 %), iso-C16:0 (18.5 %), summed feature 9 (comprising iso-C17:1 ω9c and/or C16:0 10-methyl; 8.8 %), C16:1 ω7c alcohol (8.8 %), iso-C11:0 3-OH (6.9 %) and iso-C11:0 (6.8 %). The DNA G+C content was 66.1 mol %. The only polyamine was spermidine and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFJR-3T belongs to the genus Pseudoxanthomonas and was most closely related to Pseudoxanthomonas yeongjuensis KCTC 22757T (16S rRNA gene sequence similarity, 99.0 %) and Pseudoxanthomonas sacheonensis KCTC 22080T (98.0 %). The levels of 16S rRNA gene sequence similarity with respect to other Pseudoxanthomonas species with validly published names were less than 96.5 %. DNA–DNA relatedness values for strain ZFJR-3T with respect to its closely related neighbours P. yeongjuensis KCTC 22757T and P. sacheonensis KCTC 22080T were 48.7 and 36.3 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain ZFJR-3T is considered to represent a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas gei sp. nov. is proposed. The type strain is ZFJR-3T (=CCTCC AB 2013020T =KCTC 32298T). 相似文献
6.
A novel bacterium designated S-42T was isolated from stream bank soil. Cells were found to be aerobic, Gram staining-negative, oxidase-positive, catalase-negative, non-motile, non-spore-forming, rod-shaped, and yellow-pigmented. The strain can grow at 15–35 °C, pH 6.0–10.0, and at 0.5% (w/v) NaCl concentration. Urea was hydrolysed. Flexirubin-type pigments were absent. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain S-42T formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes that is distinct from various species of the genus Flavobacterium, including Flavobacterium maotaiense T9T (97.6% sequence similarity), Flavobacterium hibernum ATCC 51468T (97.4%), and Flavobacterium granuli Kw05T (97.1%). The 16S rRNA gene sequences identity between strain S-42T and other members of the genus Flavobacterium were < 97.0%. Strain S-42T contains MK-6 as sole respiratory quinone. The major polar lipids were identified as phosphatidylethanolamine and an unidentified aminolipid. The major cellular fatty acids were identified as iso-C15:0, summed feature 3 (C16:1ω7c and/or C16: 1ω6c), C16:0, anteiso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH, and iso-C15:1 G. The DNA G?+?C content of the strain was 35.8 mol%. The polyphasic characterization indicated that strain S-42T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ureilyticum sp. nov. is proposed. The type strain is S-42T (=?KEMB 9005-537T?=?KACC 19115T?=?NBRC 112683T). 相似文献
7.
A halophilic, aerobic bacterium, designated GD01T, was isolated from a mangrove forest soil near the South China Sea. Cells of strain GD01T were Gram staining positive, oxidase positive, and catalase positive. The strain was rod shaped and motile by means of peritrichous flagella and produced ellipsoidal endospores. The strain was able to grow with NaCl at concentrations of 0.5–12 % (optimum 3–5 %, w/v), at temperatures of 20–50 °C (optimum 30 °C), and at pH 6.0–8.5 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GD01T formed a cluster with O. profundus DSM 18246T (96.4 % 16S rRNA gene sequence similarity), O. caeni KCTC 13061T (95.4 %), and O. oncorhynchi JCM 12661T (94.5 %). The G+C content of strain GD01T was 38.7 mol%. The major respiratory quinone was MK-7. The major cellular fatty acids (>5 %) were anteiso-C15:0, iso-C16:0 (13.7 %), anteiso-C17:0 (12.6 %), iso-C15:0 (9.9 %), iso-C14:0 (9.5 %), and C16:0 (5.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipid, four unknown lipids, and four unknown phospholipids. Based on phenotypic characteristics, chemotaxonomic features, and phylogenetic analysis based on 16S rRNA gene sequences, the strain was identified to represent a distinct novel species in the genus Oceanobacillus, and the name proposed is Oceanobacillus halophilum sp. nov. with type train GD01T (=CCTCC AB 2012863T = KCTC 33101T). 相似文献
8.
Guizhen Li Qiliang Lai Fengqin Sun Xiupian Liu Yunbiao Xie Yaping Du Guangyu Li Zongze Shao 《Antonie van Leeuwenhoek》2014,106(2):293-300
A taxonomic study was carried out on strain 22II-S11-z7T, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase negative and catalase positive, long-rod shaped, and gliding. Growth was observed at salinities of 1–5 % and at temperatures of 10–41 °C. The isolate was capable of hydrolysing gelatin and Tween 80 and able to reduce nitrate to nitrite, but unable to degrade aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z7T belongs to the genus Aquimarina, with highest sequence similarity to Aquimarina megaterium XH134T (98.31 %), followed by Aquimarina macrocephali JAMB N27T (96.59 %); other species of the genus Aquimarina shared 93.63–96.08 % sequence similarity. The ANI value between strain 22II-S11-z7T and A. megaterium XH134T was found to be 91.86–91.81 %. The DNA–DNA hybridization estimated value between strain 22II-S11-z7T and A. megaterium XH134T was 47.7 ± 2.6 %. The principal fatty acids were identified as Summed Feature 3 (C16:1 ω7c/ω6c, as defined by the MIDI system; 8.1 %), SummedFeature 9 (iso-C17:1 ω7c/C16:110-methyl; 6.8 %), iso-C15:0 G (11.3 %), iso-C15:0 (24.9 %), iso-C16:0 (5.7 %), C16:0 (5.2 %), iso-C15:0 3OH (6.4 %) and iso-C17:0 3OH (21.5 %). The G+C content of the chromosomal DNA was determined to be 32.99 mol %. The respiratory quinone was determined to be MK-6 (100 %). Phosphatidylethanolamine, two unidentified aminolipids, five unidentified phospholipids and two unidentified lipids were found to be present. The combined genotypic and phenotypic data show that strain 22II-S11-z7T represents a novel species within the genus Aquimarina, for which the name Aquimarina atlantica sp. nov. is proposed, with the type strain 22II-S11-z7T (=MCCC 1A09239T = KCTC 42003T). 相似文献
9.
Olga I. Nedashkovskaya Andrey D. Kukhlevskiy Natalia V. Zhukova 《Current microbiology》2013,66(1):16-21
A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 6Alg 8T, was isolated from the common Pacific green alga Ulva fenestrata. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 97.6 % to Polaribacter dokdonensis DSW-5T and 92.8–96.1 % to other recognized Polaribacter species. The prevalent fatty acids of strain 6Alg 8T were iso-C15:0, iso-C15:1, iso-C15:0 2-OH, C15:0 and C15:1ω6. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unknown aminolipids and one unknown lipid. The DNA G+C content of the type strain is 31.6 mol%. The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Polaribacter, for which the name Polaribacter reichenbachii sp. nov. is proposed. The type strain is 6Alg 8T (= KCTC 23969T = KMM 6386T = LMG 26443T). 相似文献
10.
Qing Chen Hong-Mei Wang Wen Zhuang Zhong-Guan Sun Jing Chen Yan Ge Ji-guo Qiu Jian He 《Antonie van Leeuwenhoek》2018,111(11):1977-1984
Strain ZZ-8T, a Gram-negative, aerobic, non-spore-forming, non-motile, yellow-pigmented, rod-shaped bacterium, was isolated from metolachlor-contaminated soil in China. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZZ-8T is a member of the genus Flavobacterium and shows high sequence similarity to Flavobacterium humicola UCM-46T (97.2%) and Flavobacterium pedocola UCM-R36T (97.1%), and lower (<?97%) sequence similarity to other known Flavobacterium species. Chemotaxonomic analysis revealed that strain ZZ-8T possessed MK-6 as the major respiratory quinone; and iso-C15:0 (28.5%), summed feature 9 (iso-C17:1 w9c/C16:0 10-methyl, 22.9%), iso-C17:0 3-OH (17.0%), iso-C15:0 3-OH (8.9%), iso-C15:1 G (8.6%) and summed feature 3 (C16:1 w7c/C16:1 w6c, 5.7%) as the predominant fatty acids. The polar lipids of strain ZZ-8T were determined to be lipids, a glycolipid, aminolipids and phosphatidylethanolamine. Strain ZZ-8T showed low DNA–DNA relatedness with F. pedocola UCM-R36T (43.23?±?4.1%) and F. humicola UCM-46T (29.17?±?3.8%). The DNA G+C content was 43.3 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization, strain ZZ-8T is considered a novel species of the genus Flavobacterium, for which the name Flavobacterium zaozhuangense sp. nov. (type strain ZZ-8T?=?KCTC 62315 T?=?CCTCC AB 2017243T) is proposed. 相似文献
11.
Ke Xing Wei Liu Yue-Ji Zhang Guang-Kai Bian Wen-Di Zhang Tomohiko Tamura Jung-Sook Lee Sheng Qin Ji-Hong Jiang 《Antonie van Leeuwenhoek》2013,103(2):433-439
A novel actinomycete, designated strain KLBMP 1262T, was isolated from a coastal plant Dendranthema indicum (Linn.) Des Moul collected from the coastal region of Nantong, Jiangsu Province, in east China and was studied in detail for its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain KLBMP 1262T is a member of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain KLBMP 1262T is most closely related to Amycolatopsis sulphurea DSM 46092T (97.96 %), Amycolatopsis ultiminotia RP-AC36T (97.50 %) and Amycolatopsis jejuensis N7-3T (97.44 %); similarity to other type strains of the genus Amycolatopsis was less than 97.0 %. The organism was determined to have chemical and morphological features consistent with its classification in the genus Amycolatopsis such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The predominant menaquinone was determined to be MK-9 (H4). The polar lipids detected were phosphatidylmethylethanolamine, phosphatidylethanolamine, an unknown aminophospholipid, two unknown glycolipids and several unknown lipids. The major fatty acids were found to be C16:0, iso-C16:0 and iso-C15:0. DNA–DNA relatedness data, together with phenotypic differences, clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate is considered to represent a novel species, for which the name A. jiangsuensis sp. nov. is proposed. The type strain is KLBMP 1262T (=KCTC 19885 T = NBRC 108679T). 相似文献
12.
Lei Zhang Lingfang Zhu Meiru Si Changfu Li Liang Zhao Yahong Wei Xihui Shen 《Antonie van Leeuwenhoek》2014,106(2):279-285
A white-coloured bacterium, designated strain GTJR-20T, was isolated from a stem of Phytolacca acinosa Roxb. collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. The novel isolate was found to grow optimally at 28–30 °C, at pH 7.5–8.0 and in the absence of NaCl. Cells were observed to be Gram-stain positive, strictly aerobic, rod-shaped and non-motile. The predominant respiratory quinone was identified as MK-7(H4) and the major cellular fatty acids were identified as iso-C16:0 (35.8 %), C18:1 ω9c (17.7 %), C17:1 ω6c (11.0 %), C17:1 ω8c (7.8 %) and C18:3 ω6c (6, 9, 12) (7.2 %). The DNA G+C content was determined to be 71.6 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTJR-20T is a member of the genus Solirubrobacter and is closely related to Solirubrobacter phytolaccae GTGR-8T (16S rRNA gene sequence similarity, 98.4 %), Solirubrobacter soli KCTC 12628T (97.8 %), Solirubrobacter pauli KCTC 9974T (97.7 %) and Solirubrobacter ginsenosidimutans KCTC 19420T (97.6 %). No other recognized bacterial species showed more than 94.6 % 16S rRNA gene sequence similarity to the novel isolate. DNA–DNA relatedness values for strain GTJR-20T with respect to its closely related neighbours S. phytolaccae GTGR-8T, S. soli KCTC 12628T, S. pauli KCTC 9974T and S. ginsenosidimutans KCTC 19420T were 48.3 ± 8.6, 21.3 ± 5.2, 36.8 ± 6.2 and 36.0 ± 5.5 %, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain GTJR-20T is considered to represent a novel species of the genus Solirubrobacter, for which the name Solirubrobacter taibaiensis sp. nov. is proposed. The type strain is GTJR-20T (=CCTCC AB 2013308T = KCTC 29222T). 相似文献
13.
Tian-Tian Yu Ji-Cheng Yao Hong Ming Yi-Rui Yin En-Min Zhou Min-Jiao Liu Shu-Kun Tang Wen-Jun Li 《Antonie van Leeuwenhoek》2013,103(3):513-518
A Gram-stain negative aerobic bacterium, designated YIM 77924T, was isolated from a geothermally heated soil sample collected at Rehai National Park, Tengchong, Yunnan province, south-west China. Growth was found to occur from 55 to 75 °C (optimum 65 °C), pH 6.0–8.0 (optimum pH 7.0) and 0–1 % NaCl (w/v). Cells were observed to be rod-shaped and the colonies convex, circular, smooth, yellow and non-transparent. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YIM 77924T belongs to the genus Thermus. The 16S rRNA gene sequence similarity values between strain YIM 77924T and other species of the genus Thermus were all below 97 %. The polar lipids of strain YIM 77924T were determined to be aminophospholipid, phospholipid and glycolipid. The predominant respiratory quinone was determined to be MK-8 and the G+C content was 66.64 mol%. The major fatty acids identified were iso-C16:0, iso-C15:0, iso-C17:0 and C16:0. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM 77924T is proposed to represent a novel species, Thermus tengchongensis sp. nov., in the genus Thermus. The type strain is YIM 77924T (=KCTC 32025T = CCTCC AB2012063T). 相似文献
14.
Zhi-Qing You Jie Li Sheng Qin Xin-Peng Tian Fa-Zuo Wang Si Zhang Wen-Jun Li 《Antonie van Leeuwenhoek》2013,103(5):963-969
A Gram-positive bacterium, designated SCSIO 15042T, was isolated from a sediment of the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew at 20–60 °C, pH 6.0–10.0 and it could grow with up to 10 % (w/v) NaCl. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and an unknown polar lipid. The only menaquinone was determined to be MK-7. The major fatty acids were identified as C16:1 ω7c/C16:1 ω6c, C16:0, iso-C15:0, anteiso-C15:0, and iso-C16:0. The DNA G+C content of strain SCSIO 15042T was determined to be 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SCSIO 15042T to the genus Bacillus. Levels of 16S rRNA gene sequence similarities between strain SCSIO 15042T and Bacillus herbersteinensis D-1-5aT, Bacillus infantis SMC 4352-1T, Bacillus novalis LMG 21837T and Bacillus drentensis LMG 21831T were 96.2, 96.2, 96.1 and 96.1 %, respectively. Based on the evidence of the present polyphasic study, strain SCSIO 15042T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus abyssalis sp. nov. is proposed. The type strain is SCSIO 15042T (=DSM 25875T = CCTCC AB 2012074T = NBRC 109102T). 相似文献
15.
An actinomycete strain, designated strain LUSFXJT, was isolated from a soil sample obtained near the Xiangtan Manganese Mine, Central-South China and characterised using a polyphasic taxonomic approach. The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces. The DNA–DNA relatedness between this strain and two closely related type strains, Streptomyces echinatus CGMCC 4.1642T and Streptomyces lanatus CGMCC 4.137T, were 28.7 ± 0.4 and 19.9 ± 2.0%, respectively, values which are far lower than the 70% threshold for the delineation of a novel prokaryotic species. The DNA G+C content of strain LUSFXJ T is 75.0 mol%. Chemotaxonomic analysis revealed that the menaquinones of strain LUSFXJT are MK-9(H6), MK-9(H8), MK-9(H2) and MK-8(H8). The polar lipid profile of strain LUSFXJT was found to contain diphosphatidylglycerol and an unidentified polar lipid. The major cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C16:0, C16:0 and Summed feature 3. Strain LUSFXJT was found to contain meso-diaminopimelic acid as the diagnostic cell wall diamino acid and the whole cell hydrolysates were found to be rich in ribose, mannose and glucose. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, it is concluded that strain LUSFXJT represents a novel species of the genus Streptomyces, for which the name S. xiangtanensis sp. nov. is proposed. The type strain is LUSFXJT (=GDMCC 4.133T = KCTC 39829T). 相似文献
16.
A Gram-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated SMK1-12T, was isolated from a tidal flat sediment on the western coast of Korea. Phylogenetic analyses based on 16S rRNA and gyrB gene sequences showed that strain SMK1-12T belonged to the genus Shewanella, clustering with the type strain of Shewanella amazonensis. Strain SMK1-12T exhibited the highest 16S rRNA gene sequence similarity value (97.0 %) and the highest gyrB sequence similarity value (87.8 %) to S. amazonensis SB2BT, respectively. Strain SMK1-12T contained simultaneously both menaquinones and ubiquinones; the predominant menaquinone was MK-7 and the predominant ubiquinones were Q-7 and Q-8. The major fatty acids (>10 % of the total fatty acids) detected in strain SMK1-12T were the MIDI system summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c), iso-C15:0, C17:1 ω8c and C16:0. The DNA G+C content of strain SMK1-12T was 58.0 mol% and its mean DNA–DNA relatedness value with S. amazonensis ATCC 700329T was 15 ± 4.6 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SMK1-12T is distinguishable from recognized Shewanella species. On the basis of the data presented, strain SMK1-12T is considered to represent a novel Shewanella species, for which the name Shewanella litorisediminis sp. nov. is proposed. The type strain is SMK1-12T (=KCTC 23961T = CCUG 62411T). 相似文献
17.
Guang-Da Feng Song-Zhen Yang Yong-Hong Wang Guo-Zhen Zhao Ming-Rong Deng Hong-Hui Zhu 《Antonie van Leeuwenhoek》2014,105(6):1091-1097
A novel bacterial strain designated 9PNM-6T was isolated from an abandoned lead–zinc ore mine site in Meizhou, Guangdong Province, China. The isolate was found to be Gram-negative, rod-shaped, orange-pigmented, strictly aerobic, oxidase- and catalase-positive. Growth occurred at 0–4 % NaCl (w/v, optimum, 0 %), at pH 6.0–8.0 (optimum, pH 7.0) and at 15–32 °C (optimum, 28–30 °C). Phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strain 9PNM-6T belongs to the genus Sphingomonas, with the highest sequence similarities with Sphingomonas jejuensis NBRC 107775T (99.7 %), Sphingomonas koreensis KCTC 2882T (95.1 %) and Sphingomonas dokdonesis KCTC 12541T (95.1 %). The chemotaxonomic characteristics of strain 9PNM-6T were consistent with those of the genus Sphingomonas. The predominant respiratory quinone was identified as ubiquinone Q-10, the major polyamine as sym-homospermidine, and the major cellular fatty acids as C18:1 ω7c, C16:0, C16:1 ω7c and/or C16:1 ω6c and C14:0 2-OH. The major polar lipids are sphingoglycolipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatideylcholine, an unidentified phospholipid and four unidentified aminolipids. The genomic DNA G+C content of strain 9PNM-6T was determined to be 69.2 ± 0.6 mol%. Based on comparative analyses of morphological, physiological and chemotaxonomic data, and levels of DNA–DNA relatedness values, strain 9PNM-6T is considered to represent a novel species of the genus Sphingomonas, for which the name Sphingomonas gimensis sp. nov. (Type strain 9PNM-6T = GIMCC 1.655T = CGMCC 1.12671T = DSM 27569T) is proposed. 相似文献
18.
Yan-yu Meng Yun-bin Fu Meng-yu Wang Yong Zhao Qi-shun Liu Yu-guang Du 《Current microbiology》2013,66(5):487-492
A Gram-negative, aerobic, rod-shaped, motile, non-spore-forming bacterial strain, designated 13-QT, was isolated from seaside soil under the stacks of the red algae in Hainan province in China. Identification was carried out on the basis of polyphasic taxonomy. Phylogenetic analysis of 16S rRNA gene sequences showed that strain 13-QT belonged to the genus Pedobacter, and the highest similarity was 94.4 % with Pedobacter terricola KCTC 12876T. Strain 13-QT was able to grow at 10–40 °C, in pH 5.0–10.0, in the presence of 0–2.0 % NaCl. The major fatty acids were iso-C15:0 (40.4 %), summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c) (18.9 %) and iso-C17:0 3-OH (18.4 %). The predominant menaquinone was MK-7. The G+C content of the genomic DNA was 42.7 mol%. Strain 13-QT could be distinguished from the nearest phylogenetic neighbors by various chemotaxonomic and phenotypic properties. The results of the polyphasic analyses suggested that strain 13-QT should be considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter hainanensis sp. nov. is proposed. The type strain is 13-QT (=CCTCC AB 2012076T = NRRL B-59850T). 相似文献
19.
Adel Aouiche Noureddine Bouras Salim Mokrane Abdelghani Zitouni Peter Schumann Cathrin Spröer Nasserdine Sabaou Hans-Peter Klenk 《Antonie van Leeuwenhoek》2015,107(1):291-296
A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258T) to 97.8 % (Actinokineospora baliensis NBRC 104211T). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25–30 °C and 5.0–7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H4). The major fatty acids were found to be iso-C16:0, iso-C15:0, iso-C16:1 H and iso-C16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84T (=DSM 45961T = CECT 8578T). 相似文献
20.
Xiaoyi Li Liping Wang Hui Huang Qiliang Lai Zongze Shao 《Antonie van Leeuwenhoek》2014,106(6):1223-1229
A novel bacterial strain designated P3-1T was isolated from the intestinal tract contents of Pacific white shrimp (Penaeus vannamei) in Zhangpu, Fujian province, China. The isolate was found to be Gram-negative, long rod shaped, oxidase- and catalase- positive. Growth was observed at 1–7 % sea salt (w/v, optimum, 3 %), at pH 7.0–9.0 (optimum, pH 7.0) and at 10–37 °C (optimum, 28 °C). The isolate was capable of hydrolysing gelatin, casein, starch and DNA but unable to degrade Tween 20, 40, 80 and cellulose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P3-1T belongs to the genus Aquimarina, with highest sequence similarity to Aquimarina gracilis PSC32T (96.2 %), followed by Aquimarina intermedia KMM 6258T (96.1 %), Aquimarina spongiae A6T (95.9 %) and Aquimarina muelleri KMM 6020T (95.8 %). The principal cellular fatty acids were identified as iso-C15:0, iso-C17:0 3OH, C16:1 ω7c/ω6c, iso-C15:1 G, iso-C15:0 3OH, iso-C17:1 ω9c/C16:0 10-methyl and C16:0. The G+C content of the chromosomal DNA was determined to be 33.3 mol%. The respiratory quinone was determined to be MK-6 (100 %). The combined genotypic and phenotypic data show that strain P3-1T represents a novel species within the genus Aquimarina, for which the name Aquimarina penaei sp. nov. is proposed, with the type strain P3-1T (=MCCC 1A09871T = LMG 27943T). 相似文献