共查询到20条相似文献,搜索用时 31 毫秒
1.
Jin-Shun Lv Li-Na Zhang Yuan-Zhi SongXin-Feng Wang Xiao-Zhong Chu 《International biodeterioration & biodegradation》2011,65(1):258-264
In present work, the chemical composition of the petroleum ether extract (PE) and the ethyl ether extract (ETE) of Albizia julibrissin Durazz Pod and their biological activity were investigated, and the biodegradation relationship between with these chemical composition were discussed. The components of the two extracts were identified by GC and by GC-MS. ETE showed a higher level of antimicrobial activity than PE, which were judged by the disc diffusion method of determination of the minimal inhibitory concentration and the minimal bactericidal concentration. The antioxidant properties of the extracts were evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, the auto-oxidation of pyrogallol test, and compared with those of the butylated hydroxytoluene (BHT). The results showed that the antioxidant activity of ETE was higher than that of PE (p < 0.01) and lower than that of BHT (p > 0.01) in the DPPH radical scavenging assay. However, in the auto-oxidation of pyrogallol test, the antioxidant activity of PE was higher than that of ETE (p < 0.01) and lower than that of BHT (p < 0.01). Because components of the A. julibrissin pod were found to have biological activity, the pod has a potential use against industrially troublesome microorganisms as a natural source of raw material for the preparation of an antioxidant and a biocide. 相似文献
2.
Exposure of cells to ionizing radiation leads to the formation of reactive oxygen species (ROS) that are associated with radiation-induced
cytotoxicity. Because of the serious damaging potential of ROS, cells depend on the elaboration of the antioxidant defense
system (AODS), both enzymatic and nonenzymatic oxidant defense mechanisms. The deficiency in important components of the endogenous
AODS leads to the accumulation of oxidative stress inducing oxidative damage. The antioxidant enzymes superoxide dismutase
and glutathione peroxidase are key intracellular antioxidants in the metabolism of ROS.
In the current study, we investigated the potential role of these antioxidant enzymes in radioresistance during the evaluation
of the compensatory role of some exogenous micronutrients against oxidative stress
Animals were categorized into eight groups, receiving vitamin E (α-tocopherol) and/or selenium (Se) with or without whole-body
γ-irradiation (6.5 Gy).
The results indicate that antioxidant pretreatments before irradiation may have some beneficial effects against irradiation-induced
injury. The results also indicate that selenium and vitamin E act alone and in an additive fashion as radioprotecting agents.
The results further suggest that selenium confers protection in part by inducing or activating cellular free-radical scavenging
systems and by enhancing peroxide breakdown, whereas vitamin E appears to confer its protection by an alternate complementary
mechanism. 相似文献
3.
Scorei R Ciubar R Iancu C Mitran V Cimpean A Iordachescu D 《Biological trace element research》2007,118(1):27-37
Discovery of naturally occurring boron complexes with organic compounds containing hydroxyl groups, sugars, and polysaccharides,
adenosine-5-phosphate, pyridoxine, riboflavin, dehydroascorbic acid, and pyridine nucleotides led to the reassessment of the
biochemical role of boron. Boron’s anti-inflammatory actions were claimed but not yet demonstrated. This study investigated
the effects of calcium fructoborate (CF) on the human polymorphonuclear neutrophils (PMN) that play a central role in the
inflammatory response. Our results demonstrated that CF exposure induced a dose-dependent decrease in cell viability. Treatment
of PMN cells, for 24 h, with 22,500 μM CF led to a decrease in cell viability by 61.1%, an inhibition of respiratory burst
by 92.9% in the case of fMLP-stimulated cells, a diminution of intracellular level of superoxide anion with 59.3%, and a stimulation
of superoxide dismutase activity by 72% in unstimulated PMN cells. Altogether, these results suggest the antioxidant and anti-inflammatory
properties of CF. 相似文献
4.
Scorei R Ciubar R Ciofrangeanu CM Mitran V Cimpean A Iordachescu D 《Biological trace element research》2008,122(3):197-205
Recent studies suggested that boron has a chemo-preventive role in prostate cancer. In the present report, we investigated
the effects of calcium fructoborate (CF) and boric acid (BA) on activation of the apoptotic pathway in MDA-MB-231 human breast
cancer cells. Exposure to BA and CF inhibited the proliferation of breast cancer cells in a dose-dependent manner. Treatment
with CF but not BA resulted in a decrease in p53 and bcl-2 protein levels. Furthermore, after the treatment with CF, augmentation
of pro-caspase-3 protein expression, cytosolic cytochrome c level, and caspase-3 activity were observed, indicating apoptotic cell death induction. This was also demonstrated by terminal
deoxynucleotidyl transferase-mediated 2′-deoxyuridine 5′-triphosphate nick-end-labeling assay. In conclusion, our data provide
arguments to the fact that both BA and CF inhibited the growth of breast cancer cells, while only CF induced apoptosis. Additional
studies will be needed to identify the underlying mechanism responsible for the observed cellular responses to these compounds
and to determine if BA and CF may be further evaluated as chemotherapeutic agents for human cancer. 相似文献
5.
Macchioni L Davidescu M Sciaccaluga M Marchetti C Migliorati G Coaccioli S Roberti R Corazzi L Castigli E 《Journal of bioenergetics and biomembranes》2011,43(5):507-518
Most cancer cells, including GL15 glioblastoma cells, rely on glycolysis for energy supply. The effect of antiglycolytic bromopyruvate
on respiratory parameters and viability of GL15 cells was investigated. Bromopyruvate caused Δψm and MTT collapse, ATP decrease, and cell viability loss without involving apoptotic or necrotic pathways. The autophagy marker
LC3-II was increased. Δψm decrease was accompanied by reactive oxygen species (ROS) increase and cytochrome c (cyt c) disappearance, suggesting a link
between free radical generation and intramitochondrial cyt c degradation. Indeed, the free radical inducer menadione caused
a decrease in cyt c that was reversed by N-acetylcysteine. Cyt c is tightly bound to the inner mitochondrial membrane in GL15
cells, which may confer protein peroxidase activity, resulting in auto-oxidation and protein targeting to degradation in the
presence of ROS. This process is directed towards impairment of the apoptotic cyt c cascade, although cells are committed
to die. 相似文献
6.
7.
Zhong-Ji Qian BoMi Ryu Moon-Moo Kim Se-Kwon Kim 《Biotechnology and Bioprocess Engineering》2008,13(6):705-715
Seahorse, Hippocampus kuda (SH) a marine teleost fish, is well known not only for its special medicinal composition and used as one of the most famous
and expensive materials of traditional Chinese medicine. It was extracted with water (SHW), methanol (SHM), and ethanol (SHE),
respectively and evaluated by various antioxidant assays. The including reducing power, total antioxidant, DPPH radical scavenging,
hydroxyl radical scavenging, superoxide anion radical scavenging, alkyl radical scavenging, and protective effect on DNA damage
caused by hydroxyl radicals generated. Further, the ROS level was detected using a fluorescence probe, 2′,7′-dichlorofluorescin
diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular
ROS on mouse macrophages, RAW264.7 cell and inhibited myeloperoxidase (MPO) activity in human myeloid, HL60 cells, respectively.
Those various antioxidant activities were compared to standard antioxidants such as α-tocopherol. Among SHM exhibited the
highest antioxidant activity in linoleic acid system, effective reducing power, DPPH radical scavenging, hydroxyl radical
scavenging, superoxide radical scavenging, alkyl radical scavenging, inhibitory intracellular ROS, and inhibited MPO activity.
Furthermore, MTT assay showed no cytotoxicity on mouse macrophages cell (RAW264.7) and human cell lines (MRC-5, HL60, U937).
This antioxidant property depends on concentration and increasing with increased amount of extracts. The results obtained
in the present study indicated that the see horse (Hippocampus kuda Bleeker) is a potential source of natural antioxidant. 相似文献
8.
Aijaz A. Wani Ashraf Y. Rangrez Himanshu Kumar Sharmila A. Bapat C. G. Suresh Shama Barnabas 《Free radical research》2013,47(5):415-427
The mechanism of free radical production by complex I deficiency is ill-defined, although it is of significant contemporary interest. This study studied the ROS production and antioxidant defenses in children with mitochondrial NADH dehydrogenase deficiency. ROS production has remained significantly elevated in patients compared to controls. The expression of all antioxidant enzymes significantly increased at mRNA level. However, the enzyme activities did not correlate with high mRNA or protein expression. Only the activity of superoxide dismutase (SOD) was found to correlate with higher mRNA expression in patient derived cell lines. The activities of the enzymes such as glutathione peroxidase (GPx), Catalase (CAT) and glutathione-S-transferase (GST) were significantly reduced in patients (p<0.05 or p<0.01). Glutathione reductase (GR) activity and intracellular glutathione (GSH) levels were not changed. Decreased enzyme activities could be due to post-translational or oxidative modification of ROS scavenging enzymes. The information on the status of ROS and marking the alteration of ROS scavenging enzymes in peripheral lymphocytes or lymphoblast cell lines will provide a better way to design antioxidant therapies for such disorders. 相似文献
9.
Marie-Odile Parat Marie-Jeanne Richard Marie Th r se Leccia Pierre Amblard Alain Favier Jean Claude B ani 《Free radical research》1995,23(4):339-351
Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidative injury on cultured human skin fibroblasts. Dithranol, hydrogen peroxide and UV-A radiation (375 nm) were employed as oxidative stressors. The supply of manganese chloride produced an increase in cellular content of this element up to 24 fold without concomitant elevation of MnSOD activity. Nevertheless, manganese protects cells against two of the three ROS generating systems assessed, namely hydrogen peroxyde and UV-A. This protective effect depends on the concentration of manganese in the medium, 0.1 mM and 0.2 mM protect against UVA cytotoxicity, only 0.2 mM protects against H2O2 cytotoxicity. 相似文献
10.
Małgorzata Kopyra Magdalena Stachoń-Wilk Edward A. Gwóźdź 《Acta Physiologiae Plantarum》2006,28(6):525-536
Nitric oxide (NO) is a small, ubiquitous molecule, whose physiological function in plants has recently been widely investigated.
It seems that one of its pivotal properties is the antioxidant capacity, enabling plants to alleviate the effects of the oxidized
stress. In this work we investigated the role of NO in soybean (Glycine max L. Cv. Navico) cell suspension treated with cadmium. Sodium nitroprusside (SNP), nitric oxide donor, markedly decreased the
negative influence of Cd2+ on cell growth. It was also found to stimulate superoxide dismutase (SOD, EC 1.15.1.1). Using specific fluorochromes — dihydroethidine
(DHE) and 2′,7′- dichlorofluorescein (DCFH-DA) it was shown that NO was very effective in reducing the level of superoxide
anion (O
2
·−
) and hydrogen peroxide, respectively. Furthermore, as evaluated by means of NO specific fluorochrome 4,5-diaminofluorescein
diacetate (DAF-2DA), increased production of NO was found in Cd-treated cells. In cadmium-stressed cells SNP lowered the level
of oxidized proteins.
Our results suggest that the antioxidant properties of nitric oxide in Cd-treated soybean cells rely mainly on its ability
to direct scavenging of ROS and stimulation of the antioxidant system. 相似文献
11.
目的 实验研究氯仿染毒 2 4h对非洲绿猴肾细胞的损伤及其机理。方法 运用显微荧光术测定了非洲绿猴肾细胞 (Vero细胞 )内活性氧 (ROS)含量及游离Ca2 + 浓度 ,同时 ,测定Vero细胞培养上清液乳酸脱氢酶(LDH)活力用于检查Vero细胞受损情况。结果 接触浓度为 4 0mmol L氯仿的Vero细胞内ROS含量及游离Ca2 +浓度与对照组比较无显著性差异 (P >0 0 5 ) ,同时 ,表示Vero细胞受损指标 (LDH活力 )也无显著性差异 (P >0 0 5 ) ;而接触浓度为 8 0mmol L、12 0mmol L氯仿的Vero细胞内ROS含量显著高于对照组 (P <0 0 1) ,其Vero细胞受损也显著增加 (P <0 0 5、P <0 0 1)。结论 较高浓度的氯仿能损伤Vero细胞 ,其损伤的可能途径是通过提高Vero细胞内ROS含量 相似文献
12.
Hengyi Xu Feng Qu Hong Xu Weihua Lai Y. Andrew Wang Zoraida P. Aguilar Hua Wei 《Biometals》2012,25(1):45-53
In this study, the conditions and mechanism of antibacterial activity of hydrophilic polymer coated silver nanoparticles (AgNPs)
against E. coli O157:H7 (CMCC44828) as model pathogen was studied. The AgNPs were coated with amphiphilic polymer that introduced carboxyl
groups on the surface to make it water-soluble. The AgNPs were exposed to various treatment conditions of pH and temperature
before these were combined with the E. coli. The mechanism of the antibacterial activity was studied through the formation of reactive oxygen species (ROS) that was
later suppressed with antioxidant to establish correlation with the AgNPs antimicrobial activity. Studies were carried out
at both anaerobic and aerobic conditions. The results indicated that 5 mg/L AgNPs inhibited ~50% of the growth of 106 colony forming units per milliliter (cfu/mL) E. coli cells in liquid Luria–Bertani (LB) medium. This dose-dependent antimicrobial activity was higher at increased temperature
(37°C) but was lower when the AgNPs were treated with acid at pH 2 before exposure to the bacteria. It was also established
that the conditions of higher antimicrobial effect generated more ROS that was dependent on the presence of oxygen. The antibacterial
activity was suppressed in the presence of an antioxidant. 相似文献
13.
Shailendra P. Singh Manfred Klisch Donat-P. Häder Rajeshwar P. Sinha 《World journal of microbiology & biotechnology》2008,24(12):3111-3115
The induction of shinorine (mycosporine-like amino acid (MAA)) in Anabaena variabilis PCC 7937 was studied in various culture media (BGA−, BGA+, BG11 and Allen and Arnon’s). The objective was to select the most appropriate medium that can support the highest induction
of MAAs and can be used for industrial production of these UV protective substances from cyanobacteria. Also, in vivo photosynthetic
activity was measured under shinorine inducing conditions in all media. The shinorine content and photosynthetic activity
(yield) were highest (2948.73 ± 61.13 nmol/g dry wt and 0.47 ± 0.01, respectively) in BG11 medium in comparison to others
after 72 h of UV radiation. After the same duration of irradiation shinorine content was 1076.08 ± 21.77, 1320.07 ± 98.19
and 554.64 ± 16.47 nmol/g dry wt in BGA−, BGA+, and Allen and Arnon’s media, respectively. Thus, BG11 medium can be used for mass production of MAAs from cyanobacteria. 相似文献
14.
It has been documented that medical prosthetic alloys release metal ions into surrounding tissues and cause cytotoxicity,
but the mechanisms remain undefined. In that regard the cellular oxidative stress may be a common pathway in cellular responses
to metal ions. The objective of this study was to approach the hypothesis that oxidative stress mediates chromium-induced
cytotoxicity in rat calvarial osteoblasts. Osteoblasts were exposed to different concentrations of Cr6+ or Cr3+ (5–20 μM) in the presence or absence of the antioxidant N-acetyl-cysteine (NAC; 1–5 mM). Cellular viability, differentiation, and intracellular ultrastructural alterations were evaluated
by MTT assay, alkaline phosphatase (ALP) activity assay, and transmission electron microscopy. Cellular oxidative stress was
evaluated by intracellular reactive oxygen species (ROS) production. ROS production was monitored by the oxidation-sensitive
fluorescent probe 2′7′-dichlorofluorescin diacetate (DCFH-DA). A time- and concentration- dependent increased cytotoxicity,
time-dependent increased intracellular ROS production were indicated on exposure to Cr6+. Pretreatment of osteoblasts with 1–5 mM NAC afforded dose-dependent cytoprotective effects against Cr6+-induced cytotoxicity in osteoblasts. NAC decreased the level of intracellular ROS induced by Cr6+, too. While Cr3+ and NAC did not have any significant effects on osteoblasts (5–20 μM). These results suggest that oxidative stress is involved
in Cr6+-induced cytotoxicity in osteoblasts, and NAC can provide protection for osteoblasts against Cr6+-induced oxidative stress. Cr3+ (5–20 μM) have no significant cytotoxicity in osteoblasts based on the results of this study. 相似文献
15.
Wybieralska E Koza M Sroka J Czyz J Madeja Z 《Cellular & molecular biology letters》2008,13(1):103-111
The results of several experimental studies have shown that ascorbic acid inhibits tumor growth and metastasis. Ascorbic acid
is an antioxidant that acts as a scavenger for a wide range of reactive oxygen species (ROS). Both tumour metastasis and cell
migration have been correlated with the intracellular ROS level, so it was postulated that the inhibitory effect of ascorbic
acid derivatives on cell motility may be caused by scavenging of ROS. Time-lapse analyses of Walker 256 carcinosarcoma cell
migration showed that both the speed of movement and the cell displacement were inhibited by ascorbic acid applied in concentrations
ranging from 10 to 250 μM. This effect correlated with a reduction in the intracellular ROS level in WC 256 cells, suggesting
that ROS scavenging may be a mechanism responsible for the inhibition of WC 256 cell migration. However, another potent antioxidant,
N-acetyl-L-cysteine, also efficiently decreased the intracellular ROS level in WC 256 cells, but did not affect the migration
of the investigated cells. These results demonstrate that intact, unmodified ascorbic acid applied in physiologically relevant
and nontoxicconcentrations exerts an inhibitory effect on the migration of WC 256 carcinosarcoma cells, and that this may
be one of the factors responsible for the anti-metastatic activity of vitamin C. However, our data does not support the hypothesis
that the scavenging of intracellular ROS is the main mechanism in the inhibition of cancer cell migration by ascorbic acid.
Paper authored by participants of the international conference: XXXIV Winter School of the Faculty of Biochemistry, Biophysics
and Biotechnology of Jagiellonian University, Zakopane, March 7–11, 2007, “The Cell and Its Environment”. Publication costs
were covered by the organisers of this meeting. 相似文献
16.
Studies on the superoxide anion production and peroxidase activity in potato leaf cell suspension exposed to salicylic acid 总被引:1,自引:0,他引:1
It is now widely accepted that salicylic acid (SA) signaling is mediated by reactive oxygen species (ROS) production. We have
studied the effect of SA on peroxidase activity and superoxide anion production in potato leaf cell suspension. The results
show that potato cells are insensitive to low concentrations of exogenous SA (< 1 mM) and the effect is observed at 1–5 mM
SA. The cells exposed to SA exhibit higher peroxidase activity and show different peroxidase pattern when analyzed on native
gels compared to the control. Superoxide anion production is enhanced after two hours of treatment and 2.5 mM SA gives the
highest value. The results suggest peroxidase-mediated detoxification of ROS elicited by SA. 相似文献
17.
M. O. Emel’yanov Yu. A. Kim A. F. Korystova L. N. Kublik V. V. Shaposhnikova Yu. N. Korystov 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2010,4(2):212-219
The effect of a short-time (1 h) oxidative stress on multidrug resistance (MDR) of murine leukemic P388VR cells has been investigated.
We studied the production of reactive oxygen species (ROS) in cells depending on the composition of medium and the concentration
of cells and hydrogen peroxide, as well as the effect of hydrogen peroxide on MDR of cells. MDR was determined from the transport
of calcein acetoxymethyl ester out of the cells and from a change in cell sensitivity to vincristine. The amount of ROS arising
in cells was determined using 2′,7′-dichlorodihydrofluorescein diacetate (DCFH2-DA). It was shown that the rate of ROS formation in cells decreases after the addition of serum to the medium and with an
increase of the cell number. By the action of hydrogen peroxide, the amount of ROS increases directly with its concentration.
Oxidative stress generated by 30–300 μM hydrogen peroxide decreases the MDR of the cells. The effect of hydrogen peroxide
increases with the treatment duration and concentration of hydrogen peroxide. MDR determined by the criterion of the efflux
of calcein ester from cells is completely suppressed after 1-h exposure to 300 μM hydrogen peroxide. At a concentration of
hydrogen peroxide of 60 μM and treatment duration of 1 h, the sensitivity of P388VR cells to vincristine increases to reach
the sensitivity of the wild-type P388 cells. Rapid (about 1 h) suppression of MDR is caused by inhibition of the activity
of transport proteins. MDR decrease induced by oxidative stress can be used in therapy of tumors resistant to anticancer drugs. 相似文献
18.
自由基过度引起的氧化应激是多种疾病发生的因素。连翘花黄色素(forsythia flower yellow pigment, FFYP)中含有大量的抗氧化活性物质,但其对氧化应激的抵抗性仍不清楚。本文首先通过化学方法检测FFYP的体外抗氧化活性;用细胞内抗氧化活性(cellular antioxidant activity,CAA)方法检测FFYP细胞内抗氧化活性;然后以秀丽隐杆线虫(Caenorhabditis elegans,C. elegans)为模型,检测FFYP对线虫氧化应激抵抗力及体内抗氧化指标的影响;用Daf 16和Skn 1突变体线虫和qRT PCR实验探究其作用机制。研究结果表明,FFYP具有1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基清除能力,铁离子还原能力和活性氧自由基(reactive oxygen species, ROS)清除能力,并且具有浓度依赖性。用500 μmol/L的胡桃醌提供氧化应激压力时,FFYP能显著提高线虫在氧化应激下的寿命,表明FFYP可以提高线虫对氧化应激的抵抗力。进一步研究发现,FFYP可显著降低线虫体内ROS自由基含量,提高超氧化物歧化酶(superoxide dismutase, SOD)和过氧化氢酶(catalase, CAT)活性,增加还原型谷胱甘肽(glutathione, GSH)含量,表明FFYP通过提高线虫体内抗氧化防御系统活性清除自由基来提高线虫对氧化应激的抵抗力。突变体线虫实验显示,FFYP对线虫延长氧化应激下寿命的效应在Skn-1突变体线虫中完全消失,在Daf-16突变体中效应被减弱。qRT-PCR实验也显示,Daf-16和Skn-1靶基因的表达量均被提高。表明FFYP对线虫氧化应激抵抗力提高的作用是通过Daf-16和Skn-1共同作用。这预示着FFYP具有很好的抗氧化及抗应激药用价值,有潜力成为一种新的有生物活性的天然色素。 相似文献
19.
Chengpeng Zhu Fangyu Pan Lianping Ge Jing Zhou Longlong Chen Tong Zhou Rongrong Zong Xinye Xiao Nuo Dong Maomin Yang Jian-xing Ma Zuguo Liu Yueping Zhou 《PloS one》2014,9(10)
We recently demonstrated that SERPINA3K, a serine proteinase inhibitor, has antioxidant activity in the cornea. Here we investigated the antioxidant effects of SERPINA3K on the pterygial, which is partially caused by oxidative stress in pathogenesis. The head part of primary pterygial tissue was dissected and then cultured in keratinocyte serum-free defined medium (KSFM). The cultured pterygial epithelial cells (PECs) were treated with SERPINA3K. The cell proliferation and migration of PECs were measured and analyzed. Western blot and quantitative real-time polymerase chain reaction (PCR) assay were performed. It showed that SERPINA3K significantly suppressed the cell proliferation of PECs in a concentration-dependent manner, compared with cultured human conjunctival epithelial cells. SERPINA3K also inhibited the cell migration of PECs. Towards its underlying mechanism, SERPINA3K had antioxidant activities on the PECs by significantly inhibiting NADPH oxidase 4 (NOX4), which is an important enzyme of ROS generation, and by elevating the levels of key antioxidant factors of ROS: such as NAD(P)H dehydrogenase (quinone 1) (NQO1), NF-E2–related factor-2 (NRF2) and superoxide dismutases (SOD2). Meanwhile, SERPINA3K down-regulated the key effectors of Wnt signaling pathway: β-catenin, nonphospho-β-catenin, and low-density lipoprotein receptor-related protein 6 (LRP6). We provided novel evidence that SERPINA3K had inhibitory effects on pterygium and SERPINA3K played antioxidant role via regulating the ROS system and antioxidants. 相似文献
20.
ADMA induces monocyte adhesion via activation of chemokine receptors in cultured THP-1 cells 总被引:2,自引:0,他引:2
Asymmetric dimethylarginine (ADMA), an endogenous NOS inhibitor, is also an important inflammatory factor contributing to the development of atherosclerosis (AS). The present study was to test the effect of ADMA on angiotensin (Ang) II-induced monocytic adhesion. Human monocytoid cells (THP-1) or isolated peripheral blood monocyte cells (PBMCs) were incubated with Ang II (10−6 M) or exogenous ADMA (30 μM) for 4 or 24 h in the absence or presence of losartan or antioxidant PDTC. In cultured THP-1 cells, Ang II (10−6 M) for 24 h elevated the level of ADMA in the medium, upregulated the protein expression of protein arginine methyltransferase (PRMT) and decreased the activity of dimethylarginine dimethylaminohydrolase (DDAH). Both of Ang II and ADMA increased monocytic adhesion to human umbilical vein endothelial cells (HUVECs), elevated the levels of monocyte chemoattractant protein (MCP)-1, interleukin (IL)-8 and tumor necrosis factor (TNF)-α and upregulated CCR2 and CXCR2 mRNA expression, concomitantly with increase in reactive oxygen species (ROS) generation and activation of nuclear factor (NF)-κB. Pretreatment with losartan (10 μM) or PDTC (10 μM) abolished the effects mediated by Ang II or ADMA. In isolated PBMCs from healthy individuals, ADMA upregulated the expression of CXCR2 mRNA, which was attenuated by losartan (10 μM), however, ADMA had no effect on surface protein expression of CCR2. The present results suggest that ADMA may be involved in monocytic adhesion induced by Ang II via activation of chemokine receptors by ROS/NF-κB pathway. 相似文献