共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
5.
6.
7.
8.
9.
Zinc is associated with the beta subunit of DNA-dependent RNA polymerase of Bacillus subtilis. 总被引:11,自引:0,他引:11
The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzyme is tightly bound to the beta subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the beta subunit. The presence of zinc in beta is consistent with several other lines of evidence which indicate that this subunit is dirrectly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the beta subunit as well as the alpha and beta' subunits of the RNA polymerase. 相似文献
10.
11.
Promoter recognition by sigma-37 RNA polymerase from Bacillus subtilis 总被引:21,自引:0,他引:21
12.
13.
A new polypeptide associated with RNA polymerase from Bacillus subtilis during late stages of vegetative growth 总被引:7,自引:0,他引:7
DNA-dependent RNA polymerase has been purified from at various stages of vegetative cell growth. Polymerase isolated from cultures approaching the end of the logarithmic growth phase was associated with a 60,000-dalton polypeptide and was only 10–20% as active as polymerase isolated from rapidly growing cells. Appearance of this new polypeptide and the change in template activity occur prior to stage 0 of sporulation. 相似文献
14.
15.
16.
17.
18.
19.
20.