共查询到20条相似文献,搜索用时 15 毫秒
1.
Ke Cao Lirong Wang Gengrui Zhu Weichao Fang Changwen Chen Jing Luo 《Tree Genetics & Genomes》2012,8(5):975-990
The genetic diversity, population structure, and linkage disequilibrium (LD) of peaches are greatly important in genome-wide association mapping. In the current study, 104 peach landrace accessions from six Chinese geographical regions were evaluated for fruit and phenological period. The accessions were genotyped with 53 genome-wide simple sequence repeat (SSR) markers. All SSR markers were highly polymorphic across the accessions, and a total of 340 alleles were detected, including 59 private alleles. Of the six regions studied, the northern part of China as well as the middle and lower reaches of the Changjiang River were found to be the most highly diverse genetically. Based on population structure analysis, the peaches were divided into five groups, which well agreed with the geographical distribution. Of the SSR pairs in these accessions, 18.07% (P?<?0.05) were in LD. The mean r 2 value for all intrachromosomal loci pairs was 0.0149, and LD decayed at 6.01?cM. The general linear model was used to calculate the genome-wide marker-trait associations of 10 complex traits. The traits include flesh color around the stone, red pigment in the flesh, flesh texture, flesh adhesion, flesh firmness, fruit weight, chilling requirement, flowering time, ripening time, and fruit development period. These traits were estimated by analyzing the 104 landraces. Many of the associated markers were located in regions where quantitative trait loci (QTLs) were previously identified. Peach association mapping is an effective approach for identifying QTLs and may be an alternative to QTL mapping based on crosses between different lines. 相似文献
2.
Genetic diversity and population structure of a diverse set of rice germplasm for association mapping 总被引:4,自引:0,他引:4
Liang Jin Yan Lu Peng Xiao Mei Sun Harold Corke Jinsong Bao 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(3):475-487
Germplasm diversity is the mainstay for crop improvement and genetic dissection of complex traits. Understanding genetic diversity,
population structure, and the level and distribution of linkage disequilibrium (LD) in target populations is of great importance
and a prerequisite for association mapping. In this study, 100 genome-wide simple sequence repeat (SSR) markers were used
to assess genetic diversity, population structure, and LD of 416 rice accessions including landraces, cultivars and breeding
lines collected mostly in China. A model-based population structure analysis divided the rice materials into seven subpopulations.
63% of the SSR pairs in these accessions were in LD, which was mostly due to an overall population structure, since the number
of locus pairs in LD was reduced sharply within each subpopulation, with the SSR pairs in LD ranging from 5.9 to 22.9%. Among
those SSR pairs showing significant LD, the intrachromosomal LD had an average of 25–50 cM in different subpopulations. Analysis
of the phenotypic diversity of 25 traits showed that the population structure accounted for an average of 22.4% of phenotypic
variation. An example association mapping for starch quality traits using both the candidate gene mapping and genome-wide
mapping strategies based on the estimated population structure was conducted. Candidate gene mapping confirmed that the Wx and starch synthase IIa (SSIIa) genes could be identified as strongly associated with apparent amylose content (AAC) and pasting temperature (PT), respectively.
More importantly, we revealed that the Wx gene was also strongly associated with PT. In addition to the major genes, we found five and seven SSRs were associated with
AAC and PT, respectively, some of which have not been detected in previous linkage mapping studies. The results suggested
that the population may be useful for the genome-wide marker–trait association mapping. This new association population has
the potential to identify quantitative trait loci (QTL) with small effects, which will aid in dissecting complex traits and
in exploiting the rich diversity present in rice germplasm. 相似文献
3.
Association mapping is a method to test the association between molecular markers and quantitative trait loci (QTL) based
on linkage disequilibrium (LD). In this study, the collection of 108 wheat germplasm accessions form China were evaluated
for their plant heights, spike length, spikelets per spike, grains per spike, thousand kernel weight and spikelets density
in 3 years at three locations. And they were genotyped with 85 SSR markers and 40 EST-SSR markers. The population structure
was inferred on the basis of unlinked 48 SSR markers and 40 EST-SSR markers. The extent of LD on chromosome 2A was 2.3 cM.
Association of 37 SSR loci on chromosomes 2A with six agronomic traits was analysed with a mixed linear model. A total of
14 SSR loci were significantly associated with agronomic traits. Some of the associated markers were located in the QTL region
detected in previous linkage mapping analysis. Our results demonstrated that association mapping can enhance QTL information
and achieves higher resolution with short LD extent. 相似文献
4.
Association mapping of yield and its components in rice cultivars 总被引:10,自引:0,他引:10
H. A. Agrama G. C. Eizenga W. Yan 《Molecular breeding : new strategies in plant improvement》2007,19(4):341-356
To make advances in rice breeding it is important to understand the relatedness and ancestry of introduced rice accessions,
and identify SSR markers associated with agronomically important phenotypic traits, for example yield. Ninety-two rice germplasm
accessions recently introduced from seven geographic regions of Africa, Asia, and Latin America, and eleven US cultivars,
included as checks, were evaluated for yield and kernel characteristics, and genotyped with 123 SSR markers. The SSR markers
were highly polymorphic across all accessions. Population structure analysis identified eight main clusters for the accessions
which corresponded to the major geographic regions, indicating agreement between genetic and predefined populations. Linkage
disequilibrium (LD) patterns and distributions are of fundamental importance for genome-wide mapping association. LD between
linked markers decreased with distance and with a substantial drop in LD decay values between 20 and 30 cM, suggesting it
should be possible to achieve resolution down to the 25 cM level. For the 103 cultivars, the complex traits yield, kernel
width, kernel length, kernel width/length ratio, and 1000-kernel weight, were estimated by analysis of variety trial data.
The mixed linear model method was used to disclose marker-trait associations. Many of the associated markers were located
in regions where QTL had previously been identified. In conclusion, association mapping in rice is a viable alternative to
QTL mapping based on crosses between different lines. 相似文献
5.
Association mapping is an attractive method to identify QTLs in perennial horticultural crops such as citrus, as it does not need a designed cross between parental genotypes and can save time and labor to construct a segregating population. It usually requires more genetic markers than linkage-based QTL mapping owing to a lower degree of linkage disequilibrium (LD). However, recent advances in next-generation sequencing offer high-throughput, cost-effective methods, including genotyping-by-sequencing (GBS), for genotyping massive amounts of single nucleotide polymorphisms (SNPs). In this study, we performed a genome-wide association study (GWAS) of fruit-quality traits in citrus using SNPs obtained by GBS. We evaluated 110 citrus accessions, including landraces, modern cultivars, and breeding lines, for eight fruit-quality traits (fruit weight, fruit skin color, fruit surface texture, peelability, pulp firmness, segment firmness, sugar content, and acid content) during 2005 to 2012 (except 2007). GBS found 2309 SNPs, which we anchored to the clementine reference genome. We evaluated LD in the 110 accessions and confirmed that GBS gave enough SNPs to conduct GWAS. We identified seven QTLs, including four novel ones, comprising four significant QTLs for fruit weight and one QTL each for fruit skin color, pulp firmness, and segment firmness. These QTLs offer promise for use in citrus crossbreeding. 相似文献
6.
Jianjun Zhao Maria-Jo?o Paulo Diaan Jamar Ping Lou Fred van Eeuwijk Guusje Bonnema Dick Vreugdenhil Maarten Koornneef 《Génome》2007,50(10):963-973
Association mapping was used to investigate the genetic basis of variation within Brassica rapa, which is an important vegetable and oil crop. We analyzed the variation of phytate and phosphate levels in seeds and leaves and additional developmental and morphological traits in a set of diverse B. rapa accessions and tested association of these traits with AFLP markers. The analysis of population structure revealed four subgroups in the population. Trait values differed between these subgroups, thus defining associations between population structure and trait values, even for traits such as phytate and phosphate levels. Marker-trait associations were investigated both with and without taking population structure into account. One hundred and seventy markers were found to be associated with the observed traits without correction for population structure. Association analysis with correction for population structure led to the identification of 27 markers, 6 of which had known map positions; 3 of these were confirmed in additional QTL mapping studies. 相似文献
7.
Würschum T Maurer HP Kraft T Janssen G Nilsson C Reif JC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(7):1121-1131
Recent results indicate that association mapping in populations from applied plant breeding is a powerful tool to detect QTL
which are of direct relevance for breeding. The focus of this study was to unravel the genetic architecture of six agronomic
traits in sugar beet. To this end, we employed an association mapping approach, based on a very large population of 924 elite
sugar beet lines from applied plant breeding, fingerprinted with 677 single nucleotide polymorphism (SNP) markers covering
the entire genome. We show that in this population linkage disequilibrium decays within a short genetic distance and is sufficient
for the detection of QTL with a large effect size. To increase the QTL detection power and the mapping resolution a much higher
number of SNPs is required. We found that for QTL detection, the mixed model including only the kinship matrix performed best,
even in the presence of a considerable population structure. In genome-wide scans, main effect QTL and epistatic QTL were
detected for all six traits. Our full two-dimensional epistasis scan revealed that for complex traits there appear to be epistatic
master regulators, loci which are involved in a large number of epistatic interactions throughout the genome. 相似文献
8.
Navot Galpaz Itay Gonda Doron Shem‐Tov Omer Barad Galil Tzuri Shery Lev Zhangjun Fei Yimin Xu Linyong Mao Chen Jiao Rotem Harel‐Beja Adi Doron‐Faigenboim Oren Tzfadia Einat Bar Ayala Meir Uzi Sa'ar Aaron Fait Eran Halperin Merav Kenigswald Elazar Fallik Nadia Lombardi Guy Kol Gil Ronen Yosef Burger Amit Gur Ya'akov Tadmor Vitaly Portnoy Arthur A. Schaffer Efraim Lewinsohn James J. Giovannoni Nurit Katzir 《The Plant journal : for cell and molecular biology》2018,94(1):169-191
9.
Aurora Díaz Ana Montserrat Martín-Hernández Ramón Dolcet-Sanjuan Ana Garcés-Claver José María Álvarez Jordi Garcia-Mas Belén Picó Antonio José Monforte 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(9):1837-1856
Key message
Loci on LGIV, VI, and VIII of melon genome are involved in the control of fruit domestication-related traits and they are candidate to have played a role in the domestication of the crop.Abstract
The fruit of wild melons is very small (20–50 g) without edible pulp, contrasting with the large size and high pulp content of cultivated melon fruits. An analysis of quantitative trait loci (QTL) controlling fruit morphology domestication-related traits was carried out using an in vitro maintained F2 population from the cross between the Indian wild melon “Trigonus” and the western elite cultivar ‘Piel de Sapo’. Twenty-seven QTL were identified in at least two out of the three field trials. Six of them were also being detected in BC1 and BC3 populations derived from the same cross. Ten of them were related to fruit morphological traits, 12 to fruit size characters, and 5 to pulp content. The Trigonus alleles decreased the value of the characters, except for the QTL at andromonoecious gene at linkage group (LG) II, and the QTL for pulp content at LGV. QTL genotypes accounted for a considerable degree of the total phenotypic variation, reaching up to 46%. Around 66% of the QTL showed additive gene action, 19% exhibited dominance, and 25% consisted of overdominance. The regions on LGIV, VI, and VIII included the QTL with more consistent and strong effects on domestication-related traits. QTLs on those regions were validated in BC2S1, BC2S2, and BC3 families, with “Trigonus” allele decreasing the fruit morphological traits in all cases. The validated QTL could represent loci involved in melon domestication, although further experiments as genomic variation studies across wild and cultivated genotypes would be necessary to confirm this hypothesis.10.
Association of Agronomic Traits with SNP Markers in Durum Wheat (Triticum turgidum L. durum (Desf.))
Xin Hu Jing Ren Xifeng Ren Sisi Huang Salih A. I. Sabiel Mingcheng Luo Eviatar Nevo Chunjie Fu Junhua Peng Dongfa Sun 《PloS one》2015,10(6)
Association mapping is a powerful approach to detect associations between traits of interest and genetic markers based on linkage disequilibrium (LD) in molecular plant breeding. In this study, 150 accessions of worldwide originated durum wheat germplasm (Triticum turgidum spp. durum) were genotyped using 1,366 SNP markers. The extent of LD on each chromosome was evaluated. Association of single nucleotide polymorphisms (SNP) markers with ten agronomic traits measured in four consecutive years was analyzed under a mix linear model (MLM). Two hundred and one significant association pairs were detected in the four years. Several markers were associated with one trait, and also some markers were associated with multiple traits. Some of the associated markers were in agreement with previous quantitative trait loci (QTL) analyses. The function and homology analyses of the corresponding ESTs of some SNP markers could explain many of the associations for plant height, length of main spike, number of spikelets on main spike, grain number per plant, and 1000-grain weight, etc. The SNP associations for the observed traits are generally clustered in specific chromosome regions of the wheat genome, mainly in 2A, 5A, 6A, 7A, 1B, and 6B chromosomes. This study demonstrates that association mapping can complement and enhance previous QTL analyses and provide additional information for marker-assisted selection. 相似文献
11.
Thick-skinned melon called Hami melon is the most widely cultivated and exported type of melon in China, and mainly grown in Xinjiang province. Here the genetic variation of 64 melon genotypes including 43 Xinjiang Hami melon accessions was analyzed using 36 simple sequence repeat (SSR) markers yielding 145 alleles. The polymorphic information content of SSR markers ranged from 0.09 to 0.83 (average 0.45). Based on the SSR markers, the melon accessions were clustered into 2 major groups (thick and thin-skinned melons). In addition, the sub-cluster analysis based on SSR markers partitioned different botanical groups, even separating similar agronomic trait groups (Xinjiang landraces var. ameri and var. inodorus). SSR analysis showed that 4 SSR markers (CMBR150, CMCTT144, CMBR84 and CMBR12) produced polymorphic bands of different sizes between these two botanical groups. Those four molecular markers might be related to melon fruit maturing time. A considerably low level of genetic diversity was detected in Xinjiang melon accessions. Genetic distances indicated the relatively narrower genetic base but specific taxonomic status of Xinjiang landraces compared with foreign reference accessions. 相似文献
12.
As a dense map of single nucleotide polymorphism (SNP) markers are available, population-based linkage disequilibrium (LD) mapping or association study is becoming one of the major tools for identifying quantitative trait loci (QTL) and for fine gene mapping. However, in many cases, LD between the marker and trait locus is not very strong. Approaches that maximize the potential of detecting LD will be essential for the success of LD mapping of QTL. In this paper, we propose two strategies for increasing the probability of detecting LD: (1) phenotypic selection and (2) haplotype LD mapping. To provide the foundations for LD mapping of QTL under selection, we develop analytic tools for assessing the impact of phenotypic selection on allele and haplotype frequencies, and LD under three trait models: single trait locus, two unlinked trait loci, and two linked trait loci with or without epistasis. In addition to a traditional chi(2) test, which compares the difference in allele or haplotype frequencies in the selected sample and population sample, we present multiple regression methods for LD mapping of QTL, and investigate which methods are effective in employing phenotypic selection for QTL mapping. We also develop a statistical framework for investigating and comparing the power of the single marker and multilocus haplotype test for LD mapping of QTL. Finally, the proposed methods are applied to mapping QTL influencing variation in systolic blood pressure in an isolated Chinese population. 相似文献
13.
Stijn Vanderzande Diego Micheletti Michela Troggio Mark W. Davey Johan Keulemans 《Tree Genetics & Genomes》2017,13(6):125
The identification of molecular markers associated with economic and quality traits will help improve breeding for new apple (Malus × domestica Borkh.) cultivars. Tools such as the 8K apple SNP array developed by the RosBREED consortium allow for high-throughput genotyping of SNP polymorphisms within collections. However, genetic characterization and the identification of population stratification and kinship within germplasm collections is a fundamental prerequisite for identifying robust marker–trait associations. In this study, a collection of apple germplasm originally developed for plant architectural studies and consisting of both non-commercial/local and elite accessions was genotyped using the 8K apple SNP array to identify cryptic relationships between accessions, to analyze population structure and to calculate the linkage disequilibrium (LD). A total of nine pairs of synonyms and several triploids accessions were identified within the 130 accessions genotyped. In addition, most of the known parent-child relations were confirmed, and several putative, previously unknown parent-child relations were identified among the local accessions. No clear subgroups could be identified although some separation between local and elite accessions was evident. The study of LD showed a rapid decay in our collection, indicating that a larger number of SNPs is necessary to perform whole genome association mapping. Finally, an association mapping effort for architectural traits was carried out on a small number of accessions to estimate the feasibility of this approach. 相似文献
14.
Tianzhen Zhang Neng Qian Xiefei Zhu Hong Chen Sen Wang Hongxian Mei Yuanming Zhang 《PloS one》2013,8(2)
Cotton is the world’s leading cash crop, and genetic improvement of fiber yield and quality is the primary objective of cotton breeding program. In this study, we used various approaches to identify QTLs related to fiber yield and quality. Firstly, we constructed a four-way cross (4WC) mapping population with four base core cultivars, Stoneville 2B, Foster 6, Deltapine 15 and Zhongmiansuo No.7 (CRI 7), as parents in Chinese cotton breeding history and identified 83 QTLs for 11 agronomic and fiber quality traits. Secondly, association mapping of agronomical and fiber quality traits was based on 121 simple sequence repeat (SSR) markers using a general linear model (GLM). For this, 81 Gossypium hirsutum L. accessions including the four core parents and their derived cultivars were grown in seven diverse environments. Using these approaches, we successfully identified 180 QTLs significantly associated with agronomic and fiber quality traits. Among them were 66 QTLs that were identified via linkage disequilibrium (LD) and 4WC family-based linkage (FBL) mapping and by previously published family-based linkage (FBL) mapping in modern Chinese cotton cultivars. Twenty eight and 44 consistent QTLs were identified by 4WC and LD mapping, and by FBL and LD mapping methods, respectively. Furthermore, transmission and variation of QTL-alleles mapped by LD association in the three breeding periods revealed that some could be detected in almost all Chinese cotton cultivars, suggesting their stable transmission and some identified only in the four base cultivars and not in the modern cultivars, suggesting they were missed in conventional breeding. These results will be useful to conduct genomics-assisted breeding effectively using these existing and novel QTL alleles to improve yield and fiber qualities in cotton. 相似文献
15.
Association mapping has become a widely applied genomic approach to dissect the genetic architecture of complex traits. A major issue for association mapping is the need to control for the confounding effects of population structure, which is commonly done by mixed models incorporating kinship information. In this case study, we employed experimental data from a large sugar beet population to evaluate multi-locus models for association mapping. As in linkage mapping, markers are selected as cofactors to control for population structure and genetic background variation. We compared different biometric models with regard to important quantitative trait locus (QTL) mapping parameters like the false-positive rate, the QTL detection power and the predictive power for the proportion of explained genotypic variance. Employing different approaches we show that the multi-locus model, that is, incorporating cofactors, outperforms the other models, including the mixed model used as a reference model. Thus, multi-locus models are an attractive alternative for association mapping to efficiently detect QTL for knowledge-based breeding. 相似文献
16.
Most traits of interest to medical, agricultural and animal scientists show continuous variation and complex mode of inheritance. DNA-based markers are being deployed to analyse such complex traits, that are known as quantitative trait loci (QTL). In conventional QTL analysis, F2, backcross populations, recombinant inbred lines, backcross inbred lines and double haploids from biparental crosses are commonly used. Introgression lines and near isogenic lines are also being used for QTL analysis. However, such populations have major limitations like predominantly relying on the recombination events taking place in the F1 generation and mapping of only the allelic pairs present in the two parents. The second generation mapping resources like association mapping, nested association mapping and multiparent intercross populations potentially address the major limitations of available mapping resources. The potential of multiparent intercross populations in gene mapping has been discussed here. In such populations both linkage and association analysis can be conductted without encountering the limitations of structured populations. In such populations, larger genetic variation in the germplasm is accessed and various allelic and cytoplasmic interactions are assessed. For all practical purposes, across crop species, use of eight founders and a fixed population of 1000 individuals are most appropriate. Limitations with multiparent intercross populations are that they require longer time and more resource to be generated and they are likely to show extensive segregation for developmental traits, limiting their use in the analysis of complex traits. However, multiparent intercross population resources are likely to bring a paradigm shift towards QTL analysis in plant species. 相似文献
17.
McKay JK Richards JH Nemali KS Sen S Mitchell-Olds T Boles S Stahl EA Wayne T Juenger TE 《Evolution; international journal of organic evolution》2008,62(12):3014-3026
Despite compelling evidence that adaptation to local climate is common in plant populations, little is known about the evolutionary genetics of traits that contribute to climatic adaptation. A screen of natural accessions of Arabidopsis thaliana revealed Tsu-1 and Kas-1 to be opposite extremes for water-use efficiency and climate at collection sites for these accessions differs greatly. To provide a tool to understand the genetic basis of this putative adaptation, Kas-1 and Tsu-1 were reciprocally crossed to create a new mapping population. Analysis of F(3) families showed segregating variation in both delta(13)C and transpiration rate, and as expected these traits had a negative genetic correlation (r(g)=- 0.3). 346 RILs, 148 with Kas-1 cytoplasm and 198 with Tsu-1 cytoplasm, were advanced to the F(9) and genotyped using 48 microsatellites and 55 SNPs for a total of 103 markers. This mapping population was used for QTL analysis of delta(13)C using F(9) RIL means. Analysis of this reciprocal cross showed a large effect of cytoplasmic background, as well as two QTL for delta(13)C. The Kas-1 x Tsu-1 mapping population provides a powerful new resource for mapping QTL underlying natural variation and for dissecting the genetic basis of water-use efficiency differences. 相似文献
18.
Laurie CC Nickerson DA Anderson AD Weir BS Livingston RJ Dean MD Smith KL Schadt EE Nachman MW 《PLoS genetics》2007,3(8):e144
Crosses between laboratory strains of mice provide a powerful way of detecting quantitative trait loci for complex traits related to human disease. Hundreds of these loci have been detected, but only a small number of the underlying causative genes have been identified. The main difficulty is the extensive linkage disequilibrium (LD) in intercross progeny and the slow process of fine-scale mapping by traditional methods. Recently, new approaches have been introduced, such as association studies with inbred lines and multigenerational crosses. These approaches are very useful for interval reduction, but generally do not provide single-gene resolution because of strong LD extending over one to several megabases. Here, we investigate the genetic structure of a natural population of mice in Arizona to determine its suitability for fine-scale LD mapping and association studies. There are three main findings: (1) Arizona mice have a high level of genetic variation, which includes a large fraction of the sequence variation present in classical strains of laboratory mice; (2) they show clear evidence of local inbreeding but appear to lack stable population structure across the study area; and (3) LD decays with distance at a rate similar to human populations, which is considerably more rapid than in laboratory populations of mice. Strong associations in Arizona mice are limited primarily to markers less than 100 kb apart, which provides the possibility of fine-scale association mapping at the level of one or a few genes. Although other considerations, such as sample size requirements and marker discovery, are serious issues in the implementation of association studies, the genetic variation and LD results indicate that wild mice could provide a useful tool for identifying genes that cause variation in complex traits. 相似文献
19.
López-Sesé AI Staub JE Gómez-Guillamón ML 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,108(1):41-52
Genetic relationships among 125 Spanish melon (Cucumis melo L.) accessions from a Spanish germplasm collection were assessed using a standard molecular-marker array consisting of 34 random amplified polymorphic DNA (RAPD) markers bands (19 primers) and 72 reference accessions drawn from previous studies. The reference accession array consisted of a broad range [Japanese (19) Crete (17), African (15), and USA and Europe (US/EU, 21)] of horticultural groupings (Group Cantalupensis, Group Conomon, Group Inodorus, Group Flexuosus, and Group Chito), and of melon market classes (e.g., Charentais, U.S. Western and European Shipper types, Ogen, and Galia, Honeydew, and Casaba). Spanish melon accessions (largely Casaba, Group Inodorus) were genetically distinct from the reference accessions and other Group Inodorus melons of different origins. Most African accessions showed common genetic affinities, and grouped with the Group Chito and the Group Conomon accessions examined. Those accession groupings were distinct from all other accessions belonging to Group Cantalupensis, Flexuosus, and Inodorus accessions originating from Crete, Japan, Europe, and the U.S. Genetic diversity was highest in accessions of African origin and lowest in accessions of Spanish origin. Additional RAPD markers (49 primers, 141 bands) and 22 selected agronomic traits (quantitative and qualitative) were then used to assess the genetic diversity among Spanish accessions. While cluster analysis using fruit characteristics grouped accessions into cultivars, RAPD-based genetic-distance estimate did not provide consistent accession groupings either by cultivar or geographic origin. While the highest level of polymorphism was detected among melons originating from the central region of Spain, and in the Rochet cultivar, accessions from the Andalucía region and Green cultivars were comparatively less diverse. These results indicate that the Spanish melon accessions could be used to broaden the genetic base of local and foreign Casaba germplasm, to enhance the genetic diversity of U.S and European commercial melon germplasm, and to delineate collection strategies for acquisition of additional Spanish landraces.Communicated by C. MöllersMention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products that may be suitable. 相似文献
20.
Kittipat Ukoskit Ganlayarat Posudsavang Nattapat Pongsiripat Prasert Chatwachirawong Peeraya Klomsa-ard Patthinun Poomipant Somvong Tragoonrung 《Genomics》2019,111(1):1-9
Sugar-related traits are of great importance in sugarcane breeding. In the present study, quantitative trait loci (QTL) mapping validated with association mapping was used to identify expressed sequence tag-simple sequence repeats (EST-SSRs) associated with sugar-related traits. For linkage mapping, 524 EST-SSRs, 241 Amplified Fragment Length Polymorphisms, and 10 genomic SSR markers were mapped using 283 F1 progenies derived from an interspecific cross. Six regions were identified using Multiple QTL Mapping, and 14 unlinked markers using single marker analysis. Association analysis was performed on a set of 200 accessions, based on the mixed linear model. Validation of the EST-SSR markers using association mapping within the target QTL genomic regions identified two EST-SSR markers showing a putative relationship with uridine diphosphate (UDP) glycosyltransferase, and beta-amylase, which are associated with pol and sugar yield. These functional markers can be used for marker-assisted selection of sugarcane. 相似文献