共查询到20条相似文献,搜索用时 31 毫秒
1.
Tracy B. L.; Ivey F. M.; Hurlbut D.; Martel G. F.; Lemmer J. T.; Siegel E. L.; Metter E. J.; Fozard J. L.; Fleg J. L.; Hurley B. F. 《Journal of applied physiology》1999,86(1):195-201
To determine theeffects of strength training (ST) on muscle quality (MQ,strength/muscle volume of the trained muscle group), 12 healthy oldermen (69 ± 3 yr, range 65-75 yr) and 11 healthy older women (68 ± 3 yr, range 65-73 yr) were studied before and after aunilateral leg ST program. After a warm-up set, four sets ofheavy-resistance knee extensor ST exercise were performed 3 days/wk for9 wk on the Keiser K-300 leg extension machine. The men exhibitedgreater absolute increases in the knee extension one-repetition maximum(1-RM) strength test (75 ± 2 and 94 ± 3 kg before andafter training, respectively) and in quadriceps muscle volume measuredby magnetic resonance imaging (1,753 ± 44 and 1,955 ± 43 cm3) than the women (42 ± 2 and 55 ± 3 kg for the 1-RM test and 1,125 ± 53 vs.1,261 ± 65 cm3 forquadriceps muscle volume before and after training, respectively, inwomen; both P < 0.05). However,percent increases were similar for men and women in the 1-RM test (27 and 29% for men and women, respectively), muscle volume (12% forboth), and MQ (14 and 16% for men and women, respectively).Significant increases in MQ were observed in both groups in the trainedleg (both P < 0.05) and in the 1-RMtest for the untrained leg (both P < 0.05), but no significant differences were observed between groups,suggesting neuromuscular adaptations in both gender groups. Thus,although older men appear to have a greater capacity for absolutestrength and muscle mass gains than older women in response to ST, the relative contribution of neuromuscular and hypertrophic factors to theincrease in strength appears to be similar between genders. 相似文献
2.
Edwards N.; Wilcox I.; Polo O. J.; Sullivan C. E. 《Journal of applied physiology》1996,81(5):2142-2146
Edwards, N., I. Wilcox, O. J. Polo, and C. E. Sullivan.Hypercapnic blood pressure response is greater during the luteal phase of the menstrual cycle. J. Appl.Physiol. 81(5): 2142-2146, 1996.We investigatedthe cardiovascular responses to acute hypercapnia during the menstrualcycle. Eleven female subjects with regular menstrual cycles performedhypercapnic rebreathing tests during the follicular and luteal phasesof their menstrual cycles. Ventilatory and cardiovascular variableswere recorded breath by breath. Serum progesterone and estradiol weremeasured on each occasion. Serum progesterone was higher during theluteal [50.4 ± 9.6 (SE) nmol/l] than during thefollicular phase (2.1 ± 0.7 nmol/l;P < 0.001), but serum estradiol didnot differ (follicular phase, 324 ± 101 pmol/l; luteal phase, 162 ± 71 pmol/l; P = 0.61). Thesystolic blood pressure responses during hypercapnia were 2.0 ± 0.3 and 4.0 ± 0.5 mmHg/Torr (1 Torr = 1 mmHg rise inend-tidal PCO2) during the follicularand luteal phases, respectively, of the menstrual cycle(P < 0.01). The diastolic bloodpressure responses were 1.1 ± 0.2 and 2.1 ± 0.3 mmHg/Torrduring the follicular and luteal phases, respectively(P < 0.002). Heart rate responses did not differ during the luteal (1.7 ± 0.3 beats · min1 · Torr1)and follicular phases (1.4 ± 0.3 beats · min1 · Torr1;P = 0.59). These data demonstrate agreater pressor response during the luteal phase of the menstrual cyclethat may be related to higher serum progesterone concentrations. 相似文献
3.
K+ supplementation increases muscle [Na+-K+-ATPase] and improves extrarenal K+ homeostasis in rats 总被引:1,自引:0,他引:1
Bundgaard Henning; Schmidt Thomas A.; Larsen Jim S.; Kjeldsen Keld 《Journal of applied physiology》1997,82(4):1136-1144
Bundgaard, Henning, Thomas A. Schmidt, Jim S. Larsen, andKeld Kjeldsen. K+supplementation increases muscle[Na+-K+-ATPase]and improves extrarenal K+homeostasis in rats. J. Appl. Physiol.82(4): 1136-1144, 1997.Effects ofK+ supplementation (~200 mmolKCl/100 g chow) on plasma K+,K+ content, andNa+-K+-adeonsinetriphosphatase(ATPase) concentration([Na+-K+-ATPase])in skeletal muscles as well as on extrarenalK+ clearance were evaluated inrats. After 2 days of K+supplementation, hyperkalemia prevailed(K+-supplemented vs.weight-matched control animals) [5.1 ± 0.2 (SE) vs. 3.2 ± 0.1 mmol/l, P < 0.05, n = 5-6], and after 4 daysa significant increase in K+content was observed in gastrocnemius muscle (104 ± 2 vs. 97 ± 1 µmol/g wet wt, P < 0.05, n = 5-6). After 7 days ofK+ supplementation, a significantincrease in[3H]ouabain bindingsite concentration (344 ± 5 vs. 239 ± 8 pmol/g wet wt,P < 0.05, n = 4) was observed in gastrocnemiusmuscle. After 2 wk, increases in plasmaK+,K+ content, and[3H]ouabain bindingsite concentration in gastrocnemius muscle amounted to 40, 8, and 68%(P < 0.05) above values observed inweight-matched control animals, respectively. The latter change wasconfirmed by K+-dependentp-nitrophenyl phosphatase activitymeasurements. Fasting for 1 day reduced plasmaK+ andK+ content in gastrocnemius musclein rats that had been K+supplemented for 2 wk by 3.1 ± 0.3 mmol/l(P < 0.05, n = 5) and 15 ± 2 µmol/g wet wt(P < 0.05, n = 5), respectively. After induction of anesthesia, arterial plasma K+was measured during intravenous KCl infusion (0.75 mmolKCl · 100 g bodywt1 · h1).The K+-supplemented fasted groupdemonstrated a 42% (P < 0.05) lower plasma K+ rise, associated with asignificantly higher increase inK+ content in gastrocnemius muscleof 7 µmol/g wet wt (P < 0.05, n = 5) compared with their controlanimals. In conclusion, K+supplementation increases plasmaK+,K+ content, and[Na+-K+-ATPase]in skeletal muscles and improves extrarenalK+ clearance capacity. 相似文献
4.
Turcotte Lorraine P.; Petry Carsten; Kiens Bente; Richter Erik A. 《Journal of applied physiology》1998,84(5):1788-1794
To evaluate the effects of contractions on thekinetics of uptake and oxidation of palmitate in a physiological musclepreparation, rat hindquarters were perfused with glucose (6 mmol/l),albumin-bound [1-14C]palmitate, andvarying amounts of albumin-bound palmitate (200-2,200 µmol/l) atrest and during muscle contractions. When plotted against the unboundpalmitate concentration, palmitate uptake and oxidation displayedsimple Michaelis-Menten kinetics with estimated maximal velocity(Vmax)and Michaelis-Menten constant(Km) values of42.8 ± 3.8 (SE)nmol · min1 · g1and 13.4 ± 3.4 nmol/l for palmitate uptake and 3.8 ± 0.4 nmol · min1 · g1and 8.1 ± 2.9 nmol/l for palmitate oxidation, respectively, at rest.Whereas muscle contractions increased theVmaxfor both palmitate uptake and oxidation to 91.6 ± 10.1 and 16.5 ± 2.3 nmol · min1 · g1,respectively, theKm remainedunchanged.Vmaxand Km estimates obtained from Hanes-Woolf plots (substrate concentration/velocity vs.substrate concentration) were not significantly different. In theresting perfused hindquarter, an increase in palmitate delivery from31.9 ± 0.9 to 48.7 ± 1.2 µmol · g1 · h1by increasing perfusate flow was associated with a decrease in thefractional uptake of palmitate so that the rates of uptake andoxidation of palmitate remained unchanged. It is concluded that therates of uptake and oxidation of long-chain fatty acids (LCFA) saturatewith an increase in the concentration of unbound LCFA in perfusedskeletal muscle and that muscle contractions, but not an increase inplasma flow, increase theVmaxfor LCFA uptake and oxidation. The data are consistent with the notion that uptake of LCFA in muscle may be mediated in part by a transport system. 相似文献
5.
Muscle fiber hypertrophy, hyperplasia, and capillary density in college men after resistance training 总被引:9,自引:0,他引:9
McCall G. E.; Byrnes W. C.; Dickinson A.; Pattany P. M.; Fleck S. J. 《Journal of applied physiology》1996,81(5):2004-2012
McCall, G. E., W. C. Byrnes, A. Dickinson, P. M. Pattany,and S. J. Fleck. Muscle fiber hypertrophy, hyperplasia, and capillary density in college men after resistance training.J. Appl. Physiol. 81(5):2004-2012, 1996.Twelve male subjects with recreationalresistance training backgrounds completed 12 wk of intensifiedresistance training (3 sessions/wk; 8 exercises/session; 3 sets/exercise; 10 repetitions maximum/set). All major muscle groupswere trained, with four exercises emphasizing the forearm flexors.After training, strength (1-repetition maximum preacher curl) increasedby 25% (P < 0.05). Magneticresonance imaging scans revealed an increase in the biceps brachiimuscle cross-sectional area (CSA) (from 11.8 ± 2.7 to 13.3 ± 2.6 cm2;n = 8;P < 0.05). Muscle biopsies of thebiceps brachii revealed increases(P < 0.05) in fiber areas for type I(from 4,196 ± 859 to 4,617 ± 1,116 µm2;n = 11) and II fibers (from 6,378 ± 1,552 to 7,474 ± 2,017 µm2;n = 11). Fiber number estimated fromthe above measurements did not change after training (293.2 ± 61.5 × 103 pretraining; 297.5 ± 69.5 × 103 posttraining;n = 8). However, the magnitude ofmuscle fiber hypertrophy may influence this response because thosesubjects with less relative muscle fiber hypertrophy, but similarincreases in muscle CSA, showed evidence of an increase in fibernumber. Capillaries per fiber increased significantly(P < 0.05) for both type I(from 4.9 ± 0.6 to 5.5 ± 0.7;n = 10) and II fibers (from 5.1 ± 0.8 to 6.2 ± 0.7; n = 10). Nochanges occurred in capillaries per fiber area or muscle area. Inconclusion, resistance training resulted in hypertrophy of the totalmuscle CSA and fiber areas with no change in estimated fiber number,whereas capillary changes were proportional to muscle fiber growth. 相似文献
6.
Energy requirements and physical activity in free-living older women and men: a doubly labeled water study 总被引:2,自引:0,他引:2
Starling Raymond D.; Toth Michael J.; Carpenter William H.; Matthews Dwight E.; Poehlman Eric T. 《Journal of applied physiology》1998,85(3):1063-1069
Determinants of daily energy needs and physicalactivity are unknown in free-living elderly. This study examineddeterminants of daily total energy expenditure (TEE) andfree-living physical activity in older women(n = 51; age = 67 ± 6 yr) and men(n = 48; age = 70 ± 7 yr) by usingdoubly labeled water and indirect calorimetry. Usingmultiple-regression analyses, we predicted TEE by using anthropometric,physiological, and physical activity indexes. Data were collected onresting metabolic rate (RMR), body composition, peak oxygen consumption(O2 peak),leisure time activity, and plasma thyroid hormone. Data adjusted forbody composition were not different between older women and men,respectively (in kcal/day): TEE, 2,306 ± 647 vs. 2,456 ± 666;RMR, 1,463 ± 244 vs. 1,378 ± 249; and physical activity energyexpenditure, 612 ± 570 vs. 832 ± 581. In a subgroup of 70 womenand men, RMR andO2 peakexplained approximately two-thirds of the variance in TEE(R2 = 0.62;standard error of the estimate = ±348 kcal/day). Crossvalidation ofthis equation in the remaining 29 women and men was successful, with nodifference between predicted and measured TEE (2,364 ± 398 and2,406 ± 571 kcal/day, respectively). The strongest predictors ofphysical activity energy expenditure(P < 0.05) for womenand men were O2 peak(r = 0.43), fat-free mass(r = 0.39), and body mass(r = 0.34). In summary, RMR andO2 peak are importantindependent predictors of energy requirements in the elderly.Furthermore, cardiovascular fitness and fat-free mass are moderatepredictors of physical activity in free-living elderly. 相似文献
7.
Withers R. T.; LaForgia J.; Pillans R. K.; Shipp N. J.; Chatterton B. E.; Schultz C. G.; Leaney F. 《Journal of applied physiology》1998,85(1):238-245
This study compared the traditionaltwo-compartment (fat mass or FM; fat free mass or FFM)hydrodensitometric method of body composition measurement, which isbased on body density, with three (FM, total body water or TBW, fatfree dry mass)- and four (FM, TBW, bone mineral mass or BMM,residual)-compartment models in highly trained men(n = 12), sedentary men(n = 12), highly trained women(n = 12), and sedentary women(n = 12). The means andvariances for the relative body fat (%BF) differences between the two-and three-compartment models [2.2 ± 1.6 (SD) % BF;n = 48] were significantlygreater (P 0.02) than those between the three- and four-compartment models (0.2 ± 0.3% BF;n = 48) for all four groups. Thethree-compartment model is more valid than the two-compartmenthydrodensitometric model because it controls for biological variabilityin TBW, but additional control for interindividual variability in BMMvia the four-compartment model achieves little extra accuracy. Thecombined group (n = 48) exhibited greater (P < 0.001) FFM densities(1.1075 ± 0.0049 g/cm3) thanthe hydrodensitometric assumption of 1.1000 g/cm3, which is based on analysesof three male cadavers aged 25, 35, and 46 yr. This was primarilybecause their FFM hydration (72.4 ± 1.1%;n = 48) was lower(P 0.001) than thehydrodensitometric assumption of 73.72%. 相似文献
8.
Ettinger Steven M.; Silber David H.; Gray Kristen S.; Smith Michael B.; Yang Qing X.; Kunselman Allen R.; Sinoway Lawrence I. 《Journal of applied physiology》1998,85(6):2075-2081
We comparedreflex responses to static handgrip at 30% maximal voluntarycontraction (MVC) in 10 women (mean age 24.1 ± 1.7 yr) during twophases of their ovarian cycle: the menstrual phase (days 1-4) and the follicularphase (days10-12). Changes in muscle sympathetic nerve activity (MSNA; microneurography) in response tostatic exercise were greater during the menstrual compared withfollicular phase (phase effect P = 0.01). Levels of estrogen were less during the menstrual phase(75 ± 5.5 vs. 116 ± 9.6 pg/ml, days 1-4 vs.days 10-12;P = 0.002). Generated tension did not explain differences in MSNA responses (MVC: 29.3 ± 1.3 vs. 28.2 ± 1.5 kg, days 1-4 vs.days 10-12;P = 0.13). In a group of experiments with the use of 31P-NMRspectroscopy, no phase effect was observed forH+ andH2PO4 concentrations(n = 5). During an ischemicrhythmic handgrip paradigm (20% MVC), a phase effect was notobserved for MSNA or H+ orH2PO4 concentrations,suggesting that blood flow was necessary for the expression of thecycle-related effect. The present studies suggest that, during statichandgrip exercise, MSNA is increased during the menstrual compared withthe follicular phase of the ovarian cycle. 相似文献
9.
Drink composition, voluntary drinking, and fluid balance in exercising, trained, heat-acclimatized boys 总被引:2,自引:0,他引:2
Rivera-Brown Anita M.; Gutierrez Randall; Gutierrez Juan Carlos; Frontera Walter R.; Bar-Or Oded 《Journal of applied physiology》1999,86(1):78-84
This study examinedthe effects of beverage composition on the voluntary drinking pattern,body fluid balance, and thermoregulation of heat-acclimatized trainedboys exercising intermittently in outdoor conditions (wet bulb globetemperature 30.4 ± 1.0°C). Twelve boys (age 13.4 ± 0.4 yr) performed two 3-h sessions, each consisting of four20-min cycling bouts at 60% maximal aerobic power alternating with25-min rest. One of two beverages was assigned: unflavored water (W) orflavored water plus 6% carbohydrate and 18 mmol/l Na (CNa). Drinkingwas ad libitum. Total intake was higher(P < 0.05) during CNa (1,943 ± 190 g) compared with W (1,470 ± 143 g). Euhydration wasmaintained with CNa (+0.18% body wt), but a mild dehydration resultedwith W (0.94% body wt; P < 0.05). Sweat loss, much higher than previously published for children of similar age, was similar between conditions (CNa = 1,644.7 ± 117.5; W = 1,750.2 ± 152.7 g). The increase in rectaltemperature (CNa = 0.86 ± 0.3; W = 0.76 ± 0.1°C), heartrate, and all perceptual variables did not differ between conditions.In conclusion, a flavored carbohydrate-electrolyte drink preventsvoluntary dehydration in trained heat-acclimatized boys exercising in atropical climate despite their large sweat losses. Because hydrationchanges were minor, the thermoregulatory strain observed was similarbetween conditions. 相似文献
10.
Skeletal muscle fiber quality in older men and women 总被引:15,自引:0,他引:15
Frontera WR Suh D Krivickas LS Hughes VA Goldstein R Roubenoff R 《American journal of physiology. Cell physiology》2000,279(3):C611-C618
Wholemuscle strength and cross-sectional area (WMCSA), andcontractile properties of chemically skinned segments from single fibers of the quadriceps were studied in 7 young men (YM, 36.5 ± 3.0 yr), 12 older men (OM, 74.4 ± 5.9 yr), and 12 olderwomen (OW, 72.1 ± 4.3 yr). WMCSA was smaller in OMcompared with YM (56.1 ± 10.1 vs. 79.7 ± 13.1 cm2; P = 0.031) and in OW (44.9 ± 7.5; P < 0.003) compared with OM. Age-related, but notsex-related, differences in strength were eliminated after adjustingfor WMCSA. Maximal force was measured in 552 type I and 230 type IIAfibers. Fibers from YM (type I = 725 ± 221; type IIA = 792 ± 271 µN) were stronger (P < 0.001) thanfibers from OM (I = 505 ± 179; IIA = 577 ± 262 µN) even after correcting for size. Type IIA fibers were stronger(P < 0.005) than type I fibers in YM and OM but not inOW (I = 472 ± 154; IIA = 422 ± 97 µN).Sex-related differences in type I and IIA fibers were dependent onfiber size. In conclusion, differences in WMCSA explain age-relateddifferences in strength. An intrinsic defect in contractile proteinscould explain weakness in single fibers from OM. Sex-relateddifferences exist at the whole muscle and single fiber levels. 相似文献
11.
Changes in maximum oxygen uptake during prolonged training, overtraining, and detraining in horses 总被引:2,自引:0,他引:2
Tyler Catherine M.; Golland Lorraine C.; Evans David L.; Hodgson David R.; Rose Reuben J. 《Journal of applied physiology》1996,81(5):2244-2249
Tyler, Catherine M., Lorraine C. Golland, David L. Evans,David R. Hodgson, and Reuben J. Rose. Changes in maximum oxygenuptake during prolonged training, overtraining, and detraining inhorses. J. Appl. Physiol. 81(5):2244-2249, 1996.Thirteen standardbred horses were trained asfollows: phase 1 (endurance training, 7 wk),phase 2 (high-intensity training, 9 wk),phase 3 (overload training, 18 wk), andphase 4 (detraining, 12 wk). Inphase 3, the horses were divided intotwo groups: overload training (OLT) and control (C). The OLT groupexercised at greater intensities, frequencies, and durations than groupC. Overtraining occurred after 31 wk of training and was defined as asignificant decrease in treadmill run time in response to astandardized exercise test. In the OLT group, there was a significantdecrease in body weight (P < 0.05).From pretraining values of 117 ± 2 (SE)ml · kg1 · min1,maximal O2 uptake(O2 max) increased by15% at the end of phase 1, and when signs of overtraining werefirst seen in the OLT group,O2 max was 29%higher (151 ± 2 ml · kg1 · min1in both C and OLT groups) than pretraining values. There was nosignificant reduction inO2 max until after 6 wk detraining whenO2 max was 137 ± 2 ml · kg1 · min1.By 12 wk detraining, meanO2 max was134 ± 2 ml · kg1 · min1,still 15% above pretraining values. When overtraining developed, O2 max was notdifferent between C and OLT groups, but maximal values forCO2 production (147 vs. 159 ml · kg1 · min1)and respiratory exchange ratio (1.04 vs. 1.11) were lower in the OLTgroup. Overtraining was not associated with a decrease inO2 max and, afterprolonged training, decreases inO2 max occurredslowly during detraining. 相似文献
12.
Li Yansui; Kuang Kunyan; Yerxa Benjamin; Wen Quan; Rosskothen Heinz; Fischbarg Jorge 《American journal of physiology. Cell physiology》2001,281(2):C595
Rabbit conjunctival epithelium exhibits UTP-dependentCl secretion into the tears. We investigated whetherfluid secretion also takes place. Short-circuit current(Isc) was 14.9 ± 1.4 µA/cm2(n = 16). Four P2Y2 purinergic receptoragonists [UTP and the novel compounds INS365, INS306, and INS440(Inspire Pharmaceuticals)] added apically (10 µM) resulted intemporary (~30 min) Isc increases (88%, 66%,57%, and 28%, respectively; n = 4 each). Importantly, the conjunctiva transported fluid from serosa to mucosa at a rate of6.5 ± 0.7 µl · h1 · cm2 (range2.1-15.3, n = 20). Fluid transport was stimulatedby mucosal additions of 10 µM: 1) UTP, from 7.4 ± 2.3 to 10.7 ± 3.3 µl · h1 · cm2,n = 5; and 2) INS365, from 6.3 ± 1.0 to 9.8 ± 2.5 µl · h1 · cm2,n = 5. Fluid transport was abolished by 1 mMouabain (n = 5) and was drastically inhibited by 300 µM quinidine (from 6.4 ± 1.2 to 3.6 ± 1.0 µl · h1 · cm2,n = 4). We conclude that this epithelium secretes fluidactively and that P2Y2 agonists stimulate bothCl and fluid secretions. 相似文献
13.
Smith Sinclair A.; Montain Scott J.; Matott Ralph P.; Zientara Gary P.; Jolesz Ferenc A.; Fielding Roger A. 《Journal of applied physiology》1998,85(4):1349-1356
Young[n = 5, 30 ± 5 (SD) yr] andmiddle-aged (n = 4, 58 ± 4 yr) menand women performed single-leg knee-extension exercise inside a wholebody magnetic resonance system. Two trials were performed 7 days apartand consisted of two 2-min bouts and a third bout continued toexhaustion, all separated by 3 min of recovery.31P spectra were used to determinepH and relative concentrations ofPi, phosphocreatine (PCr), and-ATP every 10 s. The subjects consumed 0.3 g · kg1 · day1of a placebo (trial 1) or creatine(trial 2) for 5 days before eachtrial. During the placebo trial, the middle-aged group had a lowerresting PCr compared with the young group (35.0 ± 5.2 vs. 39.5 ± 5.1 mmol/kg, P < 0.05) and alower mean initial PCr resynthesis rate (18.1 ± 3.5 vs. 23.2 ± 6.0 mmol · kg1 · min1,P < 0.05). After creatinesupplementation, resting PCr increased 15%(P < 0.05) in the young group and30% (P < 0.05) in the middle-aged group to 45.7 ± 7.5 vs. 45.7 ± 5.5 mmol/kg, respectively. Mean initial PCr resynthesis rate also increased in the middle-aged group(P < 0.05) to a level not differentfrom the young group (24.3 ± 3.8 vs. 24.2 ± 3.2 mmol · kg1 · min1).Time to exhaustion was increased in both groups combined after creatinesupplementation (118 ± 34 vs. 154 ± 70 s,P < 0.05). In conclusion, creatinesupplementation has a greater effect on PCr availability andresynthesis rate in middle-aged compared with youngerpersons. 相似文献
14.
Eliakim Alon; Scheett Tim; Allmendinger Nicki; Brasel Jo Anne; Cooper Dan M. 《Journal of applied physiology》2001,90(1):35-44
Little is known about the relationship among training,energy expenditure, muscle volume, and fitness in prepubertalgirls. Because physical activity is high in prepubertalchildren, we hypothesized that there would be no effect of training.Forty pre- and early pubertal (mean age 9.1 ± 0.1 yr) nonobesegirls enrolled in a 5 day/wk summer school program for 5 wk and were randomized to control (n = 20) or training groups(n = 20; 1.5 h/day, endurance-type exercise). Totalenergy expenditure (TEE) was measured using doubly labeled water, thighmuscle volume using magnetic resonance imaging, and peak O2uptake (O2 peak) using cycle ergometry.TEE was significantly greater (17%, P < 0.02) in thetraining girls. Training increased thigh muscle volume (+4.3 ± 0.9%, P < 0.005) andO2 peak (+9.5 ± 6%,P < 0.05), effects surprisingly similar to thoseobserved in adolescent girls using the same protocol (Eliakim A,Barstow TJ, Brasel JA, Ajie H, Lee W-NP, Renslo R, Berman N, and CooperDM, J Pediatr 129: 537-543, 1996). We furthercompared these two sample populations: thigh muscle volume per weightwas much lower in adolescent compared with prepubertal girls (17.0 ± 0.3 vs. 27.8 ± 0.6 ml/kg body mass; P < 0.001), and allometric analysis revealed remarkably low scaling factorsrelating muscle volume (0.34 ± 0.05, P < 0.0001), TEE (0.24 ± 0.06, P < 0.0004), andO2 peak (0.28 ± 0.07, P < 0.0001) to body mass in all subjects. Muscle andcardiorespiratory functions were quite responsive to brief training inprepubertal girls. Moreover, a retrospective, cross-sectional analysissuggests that increases in muscle mass andO2 peak may be depressed in nonobeseAmerican girls as they mature. 相似文献
15.
Laforgia J.; Withers R. T.; Shipp N. J.; Gore C. J. 《Journal of applied physiology》1997,82(2):661-666
Laforgia, J., R. T. Withers, N. J. Shipp, and C. J. Gore.Comparison of energy expenditure elevations after submaximal andsupramaximal running. J. Appl.Physiol. 82(2): 661-666, 1997.Although exerciseintensity has been identified as a major determinant of the excesspostexercise oxygen consumption (EPOC), no studies have compared theEPOC after submaximal continuous running and supramaximal intervalrunning. Eight male middle-distance runners [age = 21.1 ± 3.1 (SD) yr; mass = 67.8 ± 5.1 kg; maximal oxygen consumption(O2 max) = 69.2 ± 4.0 ml · kg1 · min1] thereforecompleted two equated treatments of treadmill running (continuousrunning: 30 min at 70%O2 max; intervalrunning: 20 × 1-min intervals at 105%O2 max withintervening 2-min rest periods) and a control session (no exercise) ina counterbalanced research design. The 9-h EPOC values were 6.9 ± 3.8 and 15.0 ± 3.3 liters (t-test:P = 0.001) for the submaximal andsupramaximal treatments, respectively. These values represent 7.1 and13.8% of the net total oxygen cost of both treatments. Notwithstanding the higher EPOC for supramaximal interval running compared with submaximal continuous running, the major contribution of both to weightloss is therefore via the energy expended during the actual exercise. 相似文献
16.
Withers R. T.; Smith D. A.; Tucker R. C.; Brinkman M.; Clark D. G. 《Journal of applied physiology》1998,84(4):1333-1340
This study examined differences betweenlong-term exercising (LE) and long-term nonexercising (LNE) women[n = 24; age 56.4 ± 6.2 (SD) yr] for resting metabolic rate (RMR) and energyexpenditure in the free-living state by using doubly labeled water(DLW). There was a statistically significant difference(P = 0.0002) between the 12 LE(94.85 ± 8.44 kJ · kg1 · day1)and 12 LNE (81.16 ± 6.62 kJ · kg1 · day1)for RMR, but this difference was only marginally significant (P = 0.06) when the data (MJ/day) weresubjected to an analysis of covariance with fat-free mass as thecovariate. The DLW data indicated that the eight most active LE(12.99 ± 3.58 MJ/day) expended significantly(P = 0.01) more energy than did theeight least active LNE (9.30 ± 1.15 MJ/day). Energy expendituresranged from 7.64 to 18.15 MJ/day, but there was no difference(P = 0.96) between the LE and LNE inenergy expenditure during activity that was not designed to eitherimprove or maintain fitness. These cross-sectional data on 49- to70-yr-old women therefore suggest that1) aerobic-type training results ina greater RMR per unit of body mass and also when statistical controlis exerted for the effect of the metabolically active fat-free mass,2) there is a large range in theenergy intake necessary to maintain energy balance, and3) aerobic training does not resultin a compensatory reduction in energy expenditure during the remainderof the day. 相似文献
17.
Microdialysis ethanol removal reflects probe recovery rather than local blood flow in skeletal muscle 总被引:2,自引:0,他引:2
Radegran G.; Pilegaard H.; Nielsen J. J.; Bangsbo J. 《Journal of applied physiology》1998,85(2):751-757
The present study compared the microdialysis ethanoloutflow-inflow technique for estimating blood flow (BF) in skeletalmuscle of humans with measurements by Doppler ultrasound of femoralartery inflow to the limb(BFFA). The microdialysis probeswere inserted in the vastus lateralis muscle and perfused with a Ringeracetate solution containing ethanol,[2-3H]adenosine (Ado),andD-[14C(U)]glucose.BFFA at rest increased from0.16 ± 0.02 to 1.80 ± 0.26 and 4.86 ± 0.53 l/minwith femoral artery infusion of Ado (AdoFA,i) at 125 and 1,000 µg · min1 · l1thigh volume (low dose and high dose, respectively;P < 0.05) and to 3.79 ± 0.37 and6.13 ± 0.65 l/min during one-legged, dynamic, thigh muscle exercisewithout and with high AdoFA,i,respectively (P < 0.05). The ethanoloutflow-to-inflow ratio (38.3 ± 2.3%) and the probe recoveries(PR) for [2-3H]Ado(35.4 ± 1.6%) and forD-[14C(U)]glucose(15.9 ± 1.1%) did not change withAdoFA,i at rest (P = not significant). During exercisewithout and with AdoFA,i, theethanol outflow-to-inflow ratio decreased(P < 0.05) to a similar level of17.5 ± 3.4 and 20.6 ± 3.2%, respectively(P = not significant), respectively,while the PR increased (P < 0.05) toa similar level (P = not significant)of 55.8 ± 2.8 and 61.2 ± 2.5% for[2-3H]Ado and to 42.8 ± 3.9 and 45.2 ± 5.1% forD-[14C(U)]glucose.Whereas the ethanol outflow-to-inflow ratio and PR correlated inverselyand positively, respectively, to the changes in BF during muscularcontractions, neither of the ratio nor PR correlated tothe AdoFA,i-induced BF increase.Thus the ethanol outflow-to-inflow ratio does not represent skeletalmuscle BF but rather contraction-induced changes in molecular transport in the interstitium or over the microdialysis membrane. 相似文献
18.
Wapnir, Raul A., Maria C. Sia, and Stanley E. Fisher.Enhancement of intestinal water absorption and sodium transport byglycerol in rats. J. Appl. Physiol.81(6): 2523-2527, 1996.Glycerol (Gly) is a hydrophilic,absorbable, and energy-rich solute that could make water absorptionmore efficient. We investigated the use of Gly in a high-energybeverage containing corn syrup (CS) by using a small intestineperfusion procedure in the rat, an approach shown earlier to providegood preclinical information. The effectiveness of several formulationswith Gly and CS was compared with commercial products and toexperimental formulas where Gly substituted for glucose (Glc). TheCS-Gly combination was more effective than preparations on the marketcontaining sucrose and Glc-fructose syrups (G-P and G-L, respectively)in maintaining a net water absorption balance in the test jejunal segment [CS-Gly = 0.021 ± 0.226, G-L = 1.516 ± 0.467, and G-P = 0.299 ± 0.106 (SE)µl · min1 · cm1(P = 0.0113)] and in reducingsodium release into the lumen [CS-Gly = 133.2 ± 16.2, G-L = 226.7 ± 25.2, and G-P = 245.6 ± 23.4 nmol · min1 · cm1(P = 0.0022)]. In otherpreparations, at equal CS concentrations (60 and 80 g/l, respectively),Gly clearly improved net water absorption over a comparableGlc-containing product [CS60-Gly = 0.422 ± 0.136 and CS80-Gly = 0.666 ± 0.378 vs. CS60-Glc = 0.282 ± 0.200 andCS80-Glc = 1.046 ± 0.480 µl · min1 · cm1(P = 0.0019)]. On the basis ofthe data of this rat intestine perfusion model, Gly could be a usefulingredient in energy-rich beverages and might enhance fluid absorptionin humans. 相似文献
19.
Pearse David B.; Wagner Elizabeth M.; Permutt Solbert 《Journal of applied physiology》1999,86(1):123-132
Ventilation during ischemia attenuatesischemia-reperfusion lung injury, but the mechanism is unknown.Increasing tissue cyclic nucleotide levels has been shown to attenuatelung ischemia-reperfusion injury. We hypothesized thatventilation prevented increased pulmonary vascular permeability duringischemia by increasing lung cyclic nucleotide concentrations.To test this hypothesis, we measured vascular permeability and cGMP andcAMP concentrations in ischemic (75 min) sheep lungs that wereventilated (12 ml/kg tidal volume) or statically inflated with the samepositive end-expiratory pressure (5 Torr). The reflection coefficientfor albumin (alb) was 0.54 ± 0.07 and 0.74 ± 0.02 (SE) in nonventilated and ventilatedlungs, respectively (n = 5, P < 0.05). Filtration coefficientsand capillary blood gas tensions were not different. The effect ofventilation was not mediated by cyclic compression of alveolarcapillaries, because negative-pressure ventilation(n = 4) also was protective (alb = 0.78 ± 0.09). Thefinal cGMP concentration was less in nonventilated than in ventilatedlungs (0.02 ± 0.02 and 0.49 ± 0.18 nmol/g blood-free dry wt,respectively, n = 5, P < 0.05). cAMP concentrations werenot different between groups or over time. Sodium nitroprussideincreased cGMP (1.97 ± 0.35 nmol/g blood-free dry wt) andalb (0.81 ± 0.09) innonventilated lungs (n = 5, P < 0.05). Isoproterenol increasedcAMP in nonventilated lungs (n = 4, P < 0.05) but had no effect onalb. The nitric oxide synthaseinhibitor NG-nitro-L-arginine methylester had no effect on lung cGMP (n = 9) or alb(n = 16) in ventilated lungs but didincrease pulmonary vascular resistance threefold(P < 0.05) in perfused sheep lungs (n = 3). These results suggest thatventilation during ischemia prevented an increase in pulmonaryvascular protein permeability, possibly through maintenance of lungcGMP by a nitric oxide-independent mechanism. 相似文献
20.
Human skeletal muscle carnitine palmitoyltransferase I activity determined in isolated intact mitochondria 总被引:2,自引:0,他引:2
Berthon Phanelie M.; Howlett Richard A.; Heigenhauser George J.F.; Spriet Lawrence L. 《Journal of applied physiology》1998,85(1):148-153
This study was designed to compare theactivity of skeletal muscle carnitine palmitoyltransferase I (CPT I) intrained and inactive men (n = 14) andwomen (n = 12). CPT Iactivity was measured in intact mitochondria, isolated from needlebiopsy vastus lateralis muscle samples (~60 mg). The variability ofCPT I activity determined on two biopsy samples from the same leg onthe same day was 4.4, whereas it was 7.0% on two biopsy samples fromthe same leg on different days. The method was sensitive to the CPT Iinhibitor malonyl-CoA (88% inhibition) and therefore specific for CPTI activity. The mean CPT I activity for all 26 subjects was 141.1 ± 10.6 µmol · min1 · kgwet muscle (wm)1 and wasnot different when all men vs. all women (140.5 ± 15.7 and 142.2 ± 14.5 µmol · min1 · kgwm1, respectively) were compared. However, CPT Iactivity was significantly higher in trained vs. inactive subjects forboth men (176.2 ± 21.1 vs. 104.1 ± 13.6 µmol · min1 · kgwm1) and women (167.6 ± 14.1 vs. 91.2 ± 9.5 µmol · min1 · kgwm1). CPT I activity was also significantly correlatedwith citrate synthase activity (all subjects,r = 0.76) and maximal oxygen consumption expressed in milliliters per kilogram per minute (all subjects, r = 0.69). Theresults of this study suggest that CPT I activity can be accurately andreliably measured in intact mitochondria isolated from human musclebiopsy samples. CPT I activity was not affected by gender, and higheractivities in aerobically trained subjects appeared to be the result ofincreased mitochondrial content in both men and women. 相似文献