共查询到20条相似文献,搜索用时 15 毫秒
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MAPK signaling up-regulates the activity of hypoxia-inducible factors by its effects on p300 总被引:23,自引:0,他引:23
Sang N Stiehl DP Bohensky J Leshchinsky I Srinivas V Caro J 《The Journal of biological chemistry》2003,278(16):14013-14019
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Two transactivation mechanisms cooperate for the bulk of HIF-1-responsive gene expression 总被引:6,自引:0,他引:6
Kasper LH Boussouar F Boyd K Xu W Biesen M Rehg J Baudino TA Cleveland JL Brindle PK 《The EMBO journal》2005,24(22):3846-3858
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Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300 总被引:13,自引:0,他引:13
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Ema M Hirota K Mimura J Abe H Yodoi J Sogawa K Poellinger L Fujii-Kuriyama Y 《The EMBO journal》1999,18(7):1905-1914
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Fu M Wang C Rao M Wu X Bouras T Zhang X Li Z Jiao X Yang J Li A Perkins ND Thimmapaya B Kung AL Munoz A Giordano A Lisanti MP Pestell RG 《The Journal of biological chemistry》2005,280(33):29728-29742
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Grimshaw SJ Mott HR Stott KM Nielsen PR Evetts KA Hopkins LJ Nietlispach D Owen D 《The Journal of biological chemistry》2004,279(3):2192-2201
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Leu-574 of HIF-1alpha is essential for the von Hippel-Lindau (VHL)-mediated degradation pathway 总被引:3,自引:0,他引:3
Huang LE Pete EA Schau M Milligan J Gu J 《The Journal of biological chemistry》2002,277(44):41750-41755
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Identification and characterization of mutations in the UPF1 gene that affect nonsense suppression and the formation of the Upf protein complex but not mRNA turnover. 总被引:13,自引:4,他引:9
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To understand the relationship between translation and mRNA decay, we have been studying how premature translation termination accelerates the degradation of mRNAs. In the yeast Saccharomyces cerevisiae, the Upf1 protein (Upf1p), which contains a cysteine- and histidine-rich region and nucleoside triphosphate hydrolysis and helicase motifs, was shown to be a trans-acting factor in this decay pathway. A UPF1 gene disruption results in the stabilization of nonsense-containing mRNAs and leads to a nonsense suppression phenotype. Biochemical analysis of the wild-type Upf1p demonstrated that it has RNA-dependent ATPase, RNA helicase, and RNA binding activities. In the work described in the accompanying paper (Y. Weng, K. Czaplinski, and S. W. Peltz, Mol. Cell. Biol. 16:5477-5490, 1996) mutations in the helicase region of Upf1p that inactivated its mRNA decay function but prevented suppression of leu2-2 and tyr7-1 nonsense alleles are identified. On the basis of these results, we suggested that Upf1p is a multifunctional protein involved in modulating mRNA decay and translation termination at nonsense codons. If this is true, we predict that UPF1 mutations with the converse phenotype should be identified. In this report, we describe the identification and biochemical characterization of mutations in the amino-terminal cysteine- and histidine-rich region of Upf1p that have normal nonsense-mediated mRNA decay activities but are able to suppress leu2-2 and tyr7-1 nonsense alleles. Biochemical characterization of these mutant proteins demonstrated that they have altered RNA binding properties. Furthermore, using the two-hybrid system, we characterized the Upf1p-Upf2p interactions and demonstrated that Upf2p interacts with Upf3p. Mutations in the cysteine- and histidine-rich region of Upf1p abolish Upf1p-Upf2p interaction. On the basis of these results, the role of the Upf complex in nonsense-mediated mRNA decay and nonsense suppression is discussed. 相似文献
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Golgin-97, RanBP2alpha, Imh1p and p230/golgin-245 (GRIP) domain golgins are targeted to the Golgi membrane through their GRIP domains. By analyzing more than 30 mutants of golgin-97 and golgin-245 GRIP domains for their properties of dimerization, interaction with ARF like protein 1 (Arl1)-GTP and Golgi targeting, we found hierarchically organized three-tier interactions governing the Golgi targeting of GRIP domain golgins. GRIP domain self-dimerization is necessary for bivalent interaction with Arl1-GTP. Unexpectedly, however, these two interactions are not sufficient for Golgi targeting, as a third group of residues, including positive-charged arginine between alpha1 and alpha2 and hydrophobic residues C-terminal to the GRIP domain, turn out to be essential. Surface plasmon resonance analysis indicates that GRIP domain interacts directly with membrane lipid, partially through the third group of residues such as W744 of golgin-97. This third tier of interaction with the membrane could be mediated by non-specific hydrophobic and electrostatic forces. 相似文献