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1.
Garrulax morrisonianus, an endemic avian species of Taiwan, inhabits evergreen forests at high elevations of 2,000–3,952 m. In this study, we developed
15 microsatellite primer pairs for genetic study. These markers were screened for 52 samples collected from wild populations
of different geographical regions. The number of alleles ranged from 4 to 13. The expected (H
E) and observed (H
O) heterozygosities were 0.430–0.725 and 0.000–0.500, respectively. All loci were significantly deviated from Hardy-Weinberg
equilibrium due to the heterozygote deficiency.
The authors Tzen-Yuh Chiang, Sao-Cheng Lao, Ya-Fu Lee contributed to the study equally. 相似文献
2.
Cheng-Yu Hung Yi-Yen Chen Tsai-Wen Hsu Tsurng-Juhn Huang Tzen-Yuh Chiang 《Conservation Genetics》2008,9(5):1337-1339
Suzukia shikikunensis Kudo is an endemic plant in Taiwan and suffers habitat destruction caused by human overexploitation. In this study, we developed
12 microsatellite primer pairs for genetic study. These markers were screened for 24 samples collected from wild populations distributed in Taiwan, and for a sister species
S. luchuensis collected from Yonaguni and Lutao Islands. The number of alleles ranged from 5 to 14. The expected (H
E) and observed (H
O) heterozygosities were 0.65–0.922 and 0–0.625, respectively. All loci were significantly deviated from Hardy–Weinberg equilibrium
due to the heterozygote deficiency. These primers amplifying microsatellites in the two species may provide a useful tool
for population genetics to establish conservation strategy. 相似文献
3.
Tsurng-Juhn Huang Yi-Yen Chen Yen-Ping Li Cheng-Yu Hung Tzen-Yuh Chiang Chang-Hung Chou 《Conservation Genetics》2008,9(5):1389-1391
Pedicularis verticillata L. is a highly valuable herb for traditional Chinese medical treatment. In this report, 11 microsatellite loci from P. verticillata were isolated. The simple sequence repeat (SSR) markers were screened in 23 samples of wild populations of P. verticillata, and eight samples from its sister P. ikomai. The number of alleles ranged from 4 to 13, and value of expected (H
E) and observed (H
0) heterozygosity was 0.62609–0.89662 and 0–0.95652, respectively. All loci were significantly deviated from Hardy–Weinberg
expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed
species. The markers amplified well in the two species are useful for examining genetic diversity and population genetic structure,
which, in turn, can provide information for establishing conservation strategy for these endangered species. 相似文献
4.
Hiroaki Setoguchi Yuki Mitsui Hajime Ikeda Naofumi Nomura Atsushi Tamura 《Conservation Genetics》2009,10(3):705-707
Nine microsatellite loci were isolated and characterized for the endangered and tertiary relict perennial herb, Tricyrtis ishiiana. The number of alleles ranged from 2 to 33. The expected (H
E) and observed (H
O) heterozygosities were 0.207–0.944 and 0.215–0.813, respectively, from 96 individuals on one population. Five loci exhibited
significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish
conservation strategy. 相似文献
5.
We developed 11 polymorphic microsatellite markers for an endangered shrub Ammopiptanthus mongolicus using the Fast Isolation by AFLP of Sequences Containing repeats protocol. Polymorphism of each locus was assessed in 24
individuals from five natural populations of A. mongolicus. The average allele number of these markers was 4.2, ranging from 2 to 7. The observed (H
O) and expected (H
E) heterozygosities were 0.044–0.956 and 0.044–0.827, respectively. Further assessment in the congeneric species A. nanus revealed that nine of eleven loci were successfully amplified, but only two loci showed polymorphism. These markers provide
powerful tools for the conservation genetics studies of A. mongolicus. 相似文献
6.
Chi-Chun Huang Kuo-Hsiang Hung Tsai-Wen Hsu Kuo-Hsiung Wang Chi-Yung Lin Tzen-Yuh Chiang 《Conservation Genetics》2008,9(5):1333-1335
Fatsia polycarpa Hayata (Araliaceae) is an element of evergreen forests at middle elevations in Taiwan, also representing the southern limitation
in distribution of the genus. In this study, we described the development of 11 microsatellite loci from F. polycarpa in Taiwan for genetic studies. These new markers were tested in 20 individuals of 5 populations of the woody species. The
number of alleles ranged from 9 to 18. The expected (H
E
) and observed (H
O
) heterozygosities were 0.80–0.95 and 0.10–0.75, respectively. Six of the 11 microsatellite loci are significantly deviated
from Hardy–Weinberg expectations likely due to the population structure within samples. No linkage disequilibrium were observed
from pairwise comparisons of loci.
Chi-Chun Huang, Kuo-Hsiang Hung, and Tsai-Wen Hsu contributed equally to this article. 相似文献
7.
Naofumi Nomura Kento Fujiwara Tokushiro Takaso Motomi Ito Koichi Uehara Hiroaki Setoguchi 《Conservation Genetics》2009,10(4):1093-1095
Eight microsatellite loci for the perennial herb Farfugium japonicum, including the rheophytic variety luchuense endemic to riparian areas of the Ryukyu Islands, Japan, were isolated and characterised. The number of alleles ranged from
5 to 14. The expected (H
E) and observed (H
O) heterozygosities were 0.344–0.885 and 0.121–0.754, respectively, from 69 individuals in one population. Six loci exhibited
significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). The primers amplifying microsatellite sequences in F. japonicum may provide a population genetics tool useful in the establishment of a conservation strategy. 相似文献
8.
Eight microsatellite loci of Calystegia soldanella useful for comparisons of the genetic structure of isolated populations in the ancient Lake Biwa and coastal populations
in Japan were isolated and characterised. The number of alleles ranged from 2 to 5. The expected (H
E) and observed (H
O) heterozygosities were 0.097–0.583 and 0.000–0.380, respectively, from 100 individuals from Lake Biwa and coastal populations.
Seven of the eight loci exhibited significantly fewer heterozygotes than expected based on the Hardy–Weinberg equilibrium
(P < 0.05). These primers amplifying microsatellites in C. soldanella may provide a population genetics tool useful in the establishment of a conservation strategy. 相似文献
9.
Tomomi Dan Yuki Mitsui Hajime Ikeda Yuji Isagi Hiroaki Setoguchi 《Conservation Genetics》2009,10(3):507-509
Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected
(H
E) and observed (H
O) heterozygosities were 0.027–0.877 and 0.027–0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus.
One locus exhibited significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish
conservation strategy. 相似文献
10.
Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae) 总被引:1,自引:0,他引:1
Kuo-Hsiang Hung Tzen-Yuh Chiang Chi-Te Chiu Tsai-Wen Hsu Chuan-Wen Ho 《Conservation Genetics》2009,10(5):1377-1380
Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine
microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of
alleles ranged from 3 to 8. The expected (H
E) and observed (H
O) heterozygosities were 0.37–0.78 and 0.05–0.52, respectively. All microsatellite loci are significantly deviated from Hardy–Weinberg
expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed
from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its
closely related species, M. floridulus. 相似文献
11.
Cristaria plicata was an important freshwater mussel for pearl culture in China. 18 polymorphic microsatellite markers were isolated and characterized
using (CA)15-enriched genomic library of C. plicata. These loci showed high levels of genetic polymorphism testing on 60 individuals sampled from Poyang Lake of Jiangxi Province,
China. The number of alleles per locus ranged from 4 to 18. The expected (H
E) and observed heterozygosities (H
O) were 0.7232–0.8961 and 0.0000–1.0000, respectively. Four microsatellite loci were significantly deviated from Hardy–Weinberg
equilibrium and no linkage disequilibrium was found. These microsatellite loci will be useful for assessment of genetic diversity
and population structure in C. plicata. 相似文献
12.
Kuo-Hsiung Wang Ming-Jou Wu Tzen-Yuh Chiang Chang-Hung Chou 《Conservation Genetics》2009,10(4):1163-1165
Euphrasia nankotaizanensis is an endangered flowering plant distributed restrictedly on rocky slope of high mountain peaks in central and northern Taiwan.
In order to undertake a conservation program, especially given impacts of the global warming, it is essential to evaluate
its genetic diversity and population structure. We described nine novel microsatellite primer pairs in E. nankotaizanensis and also examined its relative E. transmorrisonensis. The number of alleles per locus ranged from 7 to 29. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.83 to 0.98 and 0.00 to 0.95, respectively. Eight of the nine microsatellite loci displayed
significant departures from Hardy–Weinberg expectations, likely due to the loss of habitats and the small population size.
Significant linkage disequilibrium (LD) was detected in most loci. Cross-species amplification of microsatellites took place
at five loci. These primers may provide a tool for understanding the demography and population structure of Euphrasia species in Taiwan.
Kuo-Hsiung Wang, Ming-Jou Wu, and Tzen-Yuh Chiang contributed equally to this work. 相似文献
13.
Kyung-Ho Ma Anupam Dixit Young-Chang Kim Dong-Yun Lee Tae-San Kim Eun-Gi Cho Yong-Jin Park 《Conservation Genetics》2007,8(6):1507-1509
Panax ginseng C.A. Meyer, commonly known as Korean or Asian ginseng, is a perennial herb native to Korea and China. Its roots are highly
prized for several medicinal properties. The present study describes development and characterization of twenty-two polymorphic
microsatellite markers for this species. A total of 99 alleles were detected with an average of 4.5 alleles per locus across
20 accessions. Values for observed (H
O
) and expected (H
E
) heterozygosities ranged from 0.05 to 1.00 and from 0.18 to 0.73, respectively. Eleven loci deviated from Hardy–Weinberg
equilibrium (P < 0.001). Significant (P < 0.05) heterozygote deficiency was observed at 13 loci. Exact test for linkage disequilibrium showed significant values
(P < 0.05) between 12 pairs of loci. These microsatellite markers provide powerful tools for understanding population and conservation
genetics of this species and also for genetic differentiation and authentication of different Panax species being used in commercial ginseng products. 相似文献
14.
Eleven microsatellite loci have been developed from Fagus longipetiolata and the loci were characterized for 21 individuals. All eleven loci were polymorphic, with 2–8 alleles and an average of
4.8 per locus. The observed (H
O) and expected (H
E) heterozygosities were 0.053–0.714 and 0.355–0.856, respectively. There was significant deviation from Hardy–Weinberg equilibrium
at two loci. No locus pair had significant linkage disequilibrium. Cross-species amplifications of the markers were also tested
in three other congeneric species. 相似文献
15.
Chiao-Chuan Han Cheng-Sheng Chang Tzen-Yuh Chiang Ping-Han Chung Hung-Du Lin 《Conservation Genetics》2009,10(4):1065-1068
Caridina gracilipes, an atyid shrimp of Taiwan, inhabits the fresh and brackish waters. Its life history can be classified into amphidromous
and landlocked types. Recent human’s transportation has caused concerns of genetic contamination between these long evolved
lineages and ecological instabilities. Molecular markers with high sensitivities are required for detecting the possible introgression
due to human disturbance. In the study, we described the development of 15 polymorphic microsatellite loci in C. gracilipes for genetic and ecological studies. These new markers were tested in 20 individuals of three populations. The number of alleles
ranged from 6 to 17. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.653 to 0.954, averaged at 0.865, and from 0.000 to 0.4, averaged at 0.146, respectively.
All loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. Significant genetic
differentiation among populations was detected based on F
ST values, which were estimated from 0.82 to 0.88.
Chiao-Chuan Han and Cheng-Sheng Chang are contributed equally to this work. 相似文献
16.
Tzen-Yuh Chiang Teh-Wang Lee Feng-Jiau Lin Kuo-Hua Huang Hung-Du Lin 《Conservation Genetics》2008,9(5):1399-1401
Varicorhinus alticorpus (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the southern and eastern regions of Taiwan.
We described the development of nine polymorphic microsatellite loci in V. alticorpus for genetic studies. These new markers were tested in 20 individuals of the rare species. The number of alleles ranged from
4 to 13. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.692 to 0.892 (averaged at 0.821) and from 0.000 to 0.350 (averaged at 0.088), respectively.
All loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. This suite of highly
polymorphic microsatellites provides the first chance to undertake a conservation program for this species in Taiwan. It is
essential to evaluate its genetic diversity and the fine-scale population structure.
Tzen-Yuh Chiang, Teh-Wang Lee, and Feng-Jiau Lin contributed equally to this work. 相似文献
17.
Fourteen polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Liriodendron chinense (Magnoliaceae). The average allele number of these microsatellites was 5.2 per locus, ranging from three to eight. The observed
and expected heterozygosity at population level were 0.07–1.00 and 0.10–0.83, respectively. These sets of microsatellites
will be useful for studies of population genetic structure of L. chinense and L. tulipifera as well as to estimate fine-scale gene flow rates. 相似文献
18.
Tianjun Xu Changwei Shao Xiaolin Liao Xiangshan Ji Songlin Chen 《Conservation Genetics》2009,10(3):527-529
From a (GT)13-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine,
the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated
from the Hardy–Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic
in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis
and molecule-assisted breeding for O. fasciatus.
Changwei Shao contributed equally. 相似文献
19.
Kate Meares Deborah A. Dawson Gavin J. Horsburgh Mike R. Perrin Terry Burke Tiawanna D. Taylor 《Conservation Genetics》2008,9(5):1363-1367
Forty-two unique microsatellite loci were isolated from an unenriched and a tetranucleotide-enriched blue crane (Grus paradisea) genomic library. Fourteen polymorphic loci were characterised in 20 unrelated wild blue crane individuals from the Karoo
region, South Africa, and displayed 4–27 alleles with observed heterozygosities ranging between 0.50 and 0.95. All 14 loci
were also polymorphic in the grey-crowned crane (Balearica regulorum) and the wattled crane (Grus carunculatus). These markers are aimed at assisting the identification of illegal trade in the blue crane but have wider population and
conservation applications for most, if not all, crane species. 相似文献
20.
Ling Li Zheng-Feng Wang Shu-Guang Jian Peng Zhu Min Zhang Wan-Hui Ye Hai Ren 《Conservation Genetics》2009,10(3):793-795
Eight microsatellite loci were isolated from repetitive DNA enriched libraries for Cycas changjiangensis, an endangered endemic species in Hainan Island, China. The number of allele ranged from three to seven. The observed (H
O) and expected (H
E) heterozygosities ranged from 0.0000 to 0.8750 and from 0.2359 to 0.7582, respectively. These microsatellite loci will enrich
our scientific understanding for C. changjiangensis conservation. 相似文献