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1.
Melathopoulos AP Winston ML Whittington R Higo H Le Doux M 《Journal of economic entomology》2000,93(3):559-567
Neem oil, neem extract (neem-aza), and canola oil were evaluated for the management of the honey bee mite parasites Varroa jacobsoni (Oudemans) and Acarapis woodi (Rennie) in field experiments. Spraying neem oil on bees was more effective at controlling V. jacobsoni than feeding oil in a sucrose-based matrix (patty), feeding neem-aza in syrup, or spraying canola oil. Neem oil sprays also protected susceptible bees from A. woodi infestation. Only neem oil provided V. jacobsoni control comparable to the known varroacide formic acid, but it was not as effective as the synthetic product Apistan (tau-fluvalinate). Neem oil was effective only when sprayed six times at 4-d intervals and not when applied three times at 8-d intervals. Neem oil spray treatments had no effect on adult honey bee populations, but treatments reduced the amount of sealed brood in colonies by 50% and caused queen loss at higher doses. Taken together, the results suggest that neem and canola oil show some promise for managing honey bee parasitic mites, but the negative effects of treatments to colonies and the lower efficacy against V. jacobsoni compared with synthetic acaricides may limit their usefulness to beekeepers. 相似文献
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Melathopoulos AP Winston ML Whittington R Smith T Lindberg C Mukai A Moore M 《Journal of economic entomology》2000,93(2):199-209
Laboratory bioassays were conducted to evaluate neem oil and neem extract for the management of key honey bee (Apis mellifera L.) pests. Neem pesticides inhibited the growth of Paenibacillus larvae (Ash, Priest & Collins) in vitro but had no effect on the growth of Ascophaera apis (Olive & Spiltoir). Azadirachtin-rich extract (neem-aza) was 10 times more potent than crude neem oil (neem oil) against P. larvae suggesting that azadirachtin is a main antibiotic component in neem. Neem-aza, however, was ineffective at controlling the honey bee mite parasites Varroa jacobsoni (Ouduemans) and Acarapis woodi (Rennie). Honey bees also were deterred from feeding on sucrose syrup containing > 0.01 mg/ml of neem-aza. However, neem oil applied topically to infested bees in the laboratory proved highly effective against both mite species. Approximately 50-90% V. jacobsoni mortality was observed 48 h after treatment with associated bee mortality lower than 10%. Although topically applied neem oil did not result in direct A. woodi mortality, it offered significant protection of bees from infestation by A. woodi. Other vegetable and petroleum-based oils also offered selective control of honey bee mites, suggesting neem oil has both a physical and a toxicological mode of action. Although oils are not as selective as the V. jacobsoni acaricide tau-fluvalinate, they nonetheless hold promise for the simultaneous management of several honey bee pests. 相似文献
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在云南,人们有食用蜜蜂幼虫和蛹的习俗。市场上销售的蜜蜂幼虫和蛹多经热水烫过或煮过,因而缺少成虫形态,俗名易混用,食用种类长期未予明确。本文在市场调查的基础上,收集其中部分样品,通过形态学和mtDNA的COI基因部分序列初步鉴定其分类地位,以明确食用种类。结果表明,云南蜜蜂的食用集中在横断山脉南部地区,接受人群广泛,食用方式多样;云南食用本地分布的全部5种野生蜜蜂,包括东方蜜蜂(Apis cerana)、小蜜蜂(A.florea)、大蜜蜂(A.dorsata)、黑大蜜蜂(A.laboriosa)和黑小蜜蜂(A.andreniformis);基于COI序列构建的邻接法(NJ)和最大简约法(MP)系统发育树支持现有的蜜蜂属系统发育关系;现有的食用蜜蜂大多采集自野外,亟待开展遗传资源保护。本研究为食用蜜蜂的鉴定提供了数据支持,也为蜜蜂自然资源的利用和保护提供参考。 相似文献
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A laboratory bioassay was developed to evaluate miticides to control Varroa jacobsoni (Oudemans), an important parasite of the honey bee, Apis mellifera L. Bees and mites were exposed to applications of essential oil constituents in petri dishes (60 by 20 mm). The registered mite control agents tau-fluvalinate (Apistan) and formic acid also were evaluated as positive controls. Treatments that caused high mite mortality (> 70%) at doses that produced low bee mortality (< 30%) were considered mite selective. The six most selective of the 22 treatments tested (clove oil, benzyl acetate, thymol, carvacrol, methyl salicylate, and Magic3) were further evaluated to estimate LD50 values and selectivity ratios (A. mellifera LD50/V. jacobsoni LD50) at 24, 43, and 67 h after exposure. Tau-fluvalinate was the most selective treatment, but thymol, clove oil, Magic3, and methyl salicylate demonstrated selectivity equal to or greater than formic acid. The effect of mode of application (complete exposure versus vapor only) on bee and mite mortality was assessed for thymol, clove oil, and Magic3 by using a 2-chambered dish design. Estimated V. jacobsoni LD50 values were significantly lower for complete exposure applications of thymol and Magic3, suggesting that both vapor and topical exposure influenced mite mortality, whereas estimated values for clove oil suggested that topical exposure had little or no influence on mite mortality. These results indicate that essential oil constituents alone may not be selective enough to control Varroa under all conditions, but could be a useful component of an integrated pest management approach to parasitic mite management in honey bee colonies. 相似文献
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The neutral sterols of the parasitic mite Varroa jacobsoni were compared with Apis mellifera carnica drone pupae. Analysis by GLC-mass spectrometry indicated mite sterols were reflective of the sterol composition of the drones; 24-methylenecholesterol was the major sterol in both species, with lesser amounts of sitosterol and isofucosterol. Cholesterol accounted for less than 1% of the total sterols. Ecdysteroid analyses indicated drones contained primarily makisterone A. In addition to makisterone A, mites contained ecdysone and 20-hydroxyecdysone, which accounted for over 66% of the ecdysteroid detected. These results indicate that while V. jacobsoni are apparently unable to convert dietary sterols to cholesterol, they are able to produce significant amount of C(27) ecdysteroids in a low cholesterol environment. 相似文献
6.
Live Varroa jacobsoni (Mesostigmata: Varroidae) fallen from honey bee (Hymenoptera: Apidae) colonies
The proportion of Varroa jacobsoni Oudemans that were alive and mobile when they fell from honey bees, Apis mellifera L., in hives was measured during a 20-wk period to determine the potential use of systems that prevent these mites from returning to the bees. Traps designed to discriminate between the live, fallen mites and those that are dead or immobile were used on hive bottom boards. A large fraction of the fallen mites was alive when acaricide was not in use and also when fluvalinate or coumaphos treatments were in the hives. The live proportion of mitefall increased during very hot weather. The proportion of mitefall that was alive was higher at the rear and sides of the hive compared with that falling from center frames near the hive entrance. More sclerotized than callow mites were alive when they fell. A screen-covered trap that covers the entire hive bottom board requires a sticky barrier to retain all live mites. This trap or another method that prevents fallen, viable mites from returning to the hive is recommended as a part of an integrated control program. It also may slow the development of acaricide resistance in V. jacobsoni and allow the substitution of less hazardous chemicals for the acaricides currently in use. 相似文献
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The recent collection of a female of Gryphopsylla jacobsoni segregata Beaucournu & Sountsov, 1999 from northern Borneo allowed us to better clarify the taxonomic status of this flea and confirm that G. jacobsoni and G. segregata are distinct species. We describe the female of G. segregata and revised the identification key of the genus. 相似文献
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Randomly amplified polymorphic DNA (RAPD) was used to examine possible origin of Varroa jacobsoni Oudemans in the Americas. Among 64 primers screened, 2 primers provided variation which was informative for this study. All V. jacobsoni collected from the United States had the same banding pattern to that of mites collected from Russia, Morocco, Germany, Italy, Spain, and Portugal (Russian pattern). This banding pattern was different from the pattern found for mites collected from Japan, Brazil, and Puerto Rico (Japanese pattern). The Japanese pattern lacked a 766-bp band found in the Russian pattern (OPE-07). With primer OPP-03, the Russian pattern had a distinct band at 442 bp not found in the Japanese pattern. Two bands located at 675 and 412 bp were specific to the Japanese pattern. These results suggest that the V. jacobsoni of the United States is probably predominantly Russian in origin (via Europe), while the V. jacobsoni of Brazil and Puerto Rico are probably predominantly Japanese in origin. 相似文献
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狄斯瓦螨是一种严重危害西方蜜蜂的体外寄生螨,是世界养蜂业的最大威胁.人们广泛采用化学方法防治狄斯瓦螨,但易引起狄斯瓦螨的抗药性、蜜蜂中毒和蜂产品药物残留等问题.为此,人们尝试了多种蜂螨绿色防控技术.其中利用蜜蜂信息素防治狄斯瓦螨是一个重要的研究方向.研究表明,狄斯瓦螨能利用蜜蜂信息素识别处于不同发育阶段的寄主,并对特定时期的寄主表现出高度的选择性.近年来,多种能作用于狄斯瓦螨的蜜蜂信息素相继被报道.这些信息素包括成蜂、蛹和幼虫信息素.有的信息素对狄斯瓦螨表现出驱避作用,有的则表现出引诱作用.本文对这些信息素的种类、主要组成成分、对狄斯瓦螨的作用等进行了综述,旨在为今后的研究与应用提供参考. 相似文献
11.
Willem J. Boot Johan N.M. Calis Joop Beetsma Dong Minh Hai Nguyen Kim Lan Tran Van Toan Le Quang Trung Nguyen Hung Minh 《Experimental & applied acarology》1999,23(2):133-144
In colonies of European Apis mellifera, Varroa jacobsoni reproduces both in drone and in worker cells. In colonies of its original Asian host, Apis cerana, the mites invade both drone and worker brood cells, but reproduce only in drone cells. Absence of reproduction in worker cells is probably crucial for the tolerance of A. cerana towards V. jacobsoni because it implies that the mite population can only grow during periods in which drones are reared. To test if non-reproduction of V. jacobsoni in worker brood cells of A. cerana is due to a trait of the mites or of the honey-bee species, mites from bees in A. mellifera colonies were artificially introduced into A. cerana worker brood cells and vice versa. Approximately 80% of the mites from A. mellifera colonies reproduced in naturally infested worker cells as well as when introduced into worker cells of A. mellifera and A. cerana. Conversely, only 10% of the mites from A. cerana colonies reproduced, both in naturally infested worker cells of A. cerana and when introduced into worker cells of A. mellifera. Hence, absence of reproduction in worker cells is due to a trait of the mites. Additional experiments showed that A. cerana bees removed 84% of the worker brood that was artificially infested with mites from A. mellifera colonies. Brood removal started 2 days after artificial infestation, which suggests that the bees responded to behaviour of the mites. Since removal behaviour of the bees will have a large impact on fitness of the mites, it probably plays an important role in selection for differential reproductive strategies. Our findings have large implications for selection programmes to breed less-susceptible bee strains. If differences in non-reproduction are mite specific, we should not only look for non-reproduction as such, but for colonies in which non-reproduction in worker cells is selected. Hence, in selection programmes fitness of mites that reproduce in both drone and worker cells should be compared to fitness of mites that reproduce only in drone cells. © Rapid Science Ltd. 1998 相似文献
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空间诱变条件下蜜蜂后代的波动性不对称 总被引:1,自引:0,他引:1
利用航天搭载的雄蜂精液对处女王进行人工授精,产卵后大量培育后代蜂王;以SP1、SP2及SP3代蜂王后代工蜂为研究对象,考查波动性不对称(fluctuating asymmetry,FA)在不同代次种群中各对称性状间的表现情况。结果表明:SP1、SP2和SP3代工蜂的前翅长及肘脉a均表现出FA,SP1、SP2代的对照组中均未出现,而SP3代的对照组蜂群则全部表现出FA;与对照蜂群相比,空间诱变后代的前翅长及翅脉a的FA值均较高。此外,讨论FA在蜜蜂种群对生态环境适应性上应用以及利用蜜蜂的FA来监测环境污染的可能性及发展前景。 相似文献
15.
Martin C Salvy M Provost E Bagnères A Roux M Crauser D Clement J Le Conte Y 《Insect biochemistry and molecular biology》2001,31(4-5):365-379
The ectoparasitic mite Varroa jacobsoni poses a major threat to the survival of European honey-bee populations. Development of effective control methods is therefore much needed. Study of interspecific chemical communication between the parasite and host is a particularly promising avenue of research. Previous study has shown that the cuticular hydrocarbons of the parasite mite Varroa jacobsoni are qualitatively identical to those of its honey-bee host Apis mellifera (Nation J.L., Sanford M.T., Milne K., 1992. Cuticular hydrocarbons from Varroa jacobsoni. Experimental and Applied Acarology 16, 331-344). The purpose of the present study was to compare the cuticular hydrocarbon patterns of the two species at different stages of bee development. Cuticular components were identified by gas chromatography/mass spectrometry. The proportion of each component was calculated at three stages of bee development (larvae, pupa, emerging bee). The degree of chemical mimicry between the parasite and host was evaluated by multivariate analyses using the resulting proportions for each category of individuals. There were four main findings. The first was that the proportions of some components are different at the larval, pupal and imago stage of bee development. Second, Varroa profiles vary depending on the developmental stage of the host. Third, the cuticular profile of adult mites is more similar to that of the stage of the host than that of later and/or earlier stages except for parasites collected from emerging adult bees. Fourth, the degree of mimicry by Varroa is greater during larval and pupal stages than during the emerging adult bee stages. The role of chemical mimicry - although it is not perfect - in enabling parasites to infest bee colonies by the parasite is discussed. 相似文献
16.
ADRIAN G. MARSHALL 《Ecological Entomology》1977,2(4):285-291
Abstract. 1. Four species of Molossidae occur in Malaysia ( Cheiromeles torquatus and three Tadarida species) and from these, six species of potentially ectoparasitic insect have been recorded (two Etermaptera: Arixeniina; two Hemiptera: Cimicidae; two Siphonaptera: Ischnopsyllidae).
2. The two Arixeniina, Arixenia esau and Xeniaria jacobsoni are obligatorily associated with C. torquatus and only accidentally with Tadarida species with which C. torquatus often roosts. The nature of this association is presumed to be mutualistic and its origins are discussed.
3. A.esau can occur in great numbers in association with C.torquatus. It is primarily a roost dweller and is seldom found upon flying bats, although it is in this way that transference to new roosts must occur. It feeds mainly upon body exudates and skin debris from its hairless host. Aspects of its biology are discussed.
4. Arixenia species are usually found together with Xeniaria species but always greatly outnumber them. Differences between A.esau and X.jacobsoni are discussed.
5. The remaining four species of insect all feed upon blood and are truly parasitic, both species of bedbug and one flea being associated with C. torquatus and the other flea with Tadarida species. A.esau acts as a phoretic host for the flea host-specific to C. torquatus. Lagaropsylla turba. Otherwise no interactions were noted amongst the six insects. 相似文献
2. The two Arixeniina, Arixenia esau and Xeniaria jacobsoni are obligatorily associated with C. torquatus and only accidentally with Tadarida species with which C. torquatus often roosts. The nature of this association is presumed to be mutualistic and its origins are discussed.
3. A.esau can occur in great numbers in association with C.torquatus. It is primarily a roost dweller and is seldom found upon flying bats, although it is in this way that transference to new roosts must occur. It feeds mainly upon body exudates and skin debris from its hairless host. Aspects of its biology are discussed.
4. Arixenia species are usually found together with Xeniaria species but always greatly outnumber them. Differences between A.esau and X.jacobsoni are discussed.
5. The remaining four species of insect all feed upon blood and are truly parasitic, both species of bedbug and one flea being associated with C. torquatus and the other flea with Tadarida species. A.esau acts as a phoretic host for the flea host-specific to C. torquatus. Lagaropsylla turba. Otherwise no interactions were noted amongst the six insects. 相似文献
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R. A. Ilyasov A. V. Poskryakov A. V. Petukhov A. G. Nikolenko 《Russian Journal of Genetics》2016,52(3):281-291
The black honeybee Apis mellifera mellifera L. is today the only subspecies of honeybee which is suitable for commercial breeding in the climatic conditions of Northern Europe with long cold winters. The main problem of the black honeybee in Russia and European countries is the preservation of the indigenous gene pool purity, which is lost as a result of hybridization with subspecies, A. m. caucasica, A. m. carnica, A. m. carpatica, and A. m. armeniaca, introduced from southern regions. Genetic identification of the subspecies will reduce the extent of hybridization and provide the gene pool conservation of the black honeybee. Modern classification of the honeybee mitotypes is mainly based on the combined use of the DraI restriction endonuclease recognition site polymorphism and sequence polymorphism of the mtDNA COI–COII region. We performed a comparative analysis of the mtDNA COI–COII region sequence polymorphism in the honeybees of the evolutionary lineage M from Ural and West European populations of black honeybee A. m. mellifera and Spanish bee A. m. iberiensis. A new approach to the classification of the honeybee M mitotypes was suggested. Using this approach and on the basis of the seven most informative SNPs of the mtDNA COI–COII region, eight honeybee mitotype groups were identified. In addition, it is suggested that this approach will simplify the previously proposed complicated mitotype classification and will make it possible to assess the level of the mitotype diversity and to identify the mitotypes that are the most valuable for the honeybee breeding and rearing. 相似文献
18.
Peak flowering by the total flora of Africa coincides with or immediately follows peak rainfall. Flowering intensity of the total flora decreases with distance from the equator, but that of the honeybee plant resource base (±2% of total flora) does not. Flowering in the latter is highly synchronous (months 1–5 north of and 9–11 south of the equator). Both total and honeybee flora are completely incongruent with either the biomes or phytochoria of Africa. There is no significant correspondence between honeybee phenology and the total flora but significant correspondence occurs between honeybees and flowering in honeybee plant genera. A logistic regression model reveals that honeybee plant flowering predicts major honeybee colony events with a probability of 0.81 south of the equator and 0.71 for the whole continent. It is postulated that promiscuity in the bee plant genera and honeybees of Africa have contributed to their continental ubiquity. 相似文献
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Le Conte Y Mohammedi A Robinson GE 《Proceedings. Biological sciences / The Royal Society》2001,268(1463):163-168
Primer pheromones are thought to act in a variety of vertebrates and invertebrates but only a few have been chemically identified. We report that a blend of ten fatty-acid esters found on the cuticles of honeybee larvae, already known as a kairomone, releaser pheromone and primer pheromone, also act as a primer pheromone in the regulation of division of labour among adult workers. Bees in colonies receiving brood pheromone initiated foraging at significantly older ages than did bees in control colonies in five out of five trials. Laboratory and additional field tests also showed that exposure to brood pheromone significantly depressed blood titres of juvenile hormone. Brood pheromone exerted more consistent effects on age at first foraging than on juvenile hormone, suggesting that the primer effects of this pheromone may occur via other, unknown, mechanisms besides juvenile hormone. These results bring the number of social factors known to influence honeybee division of labour to three: worker-worker interactions, queen mandibular pheromone and brood pheromone. 相似文献