Molluscs and biodegradable pollution in rivers: proposal for a scale of sensitivity of species

Malacological surveys and physico-shemical water analysis have been carried out at one hundred and sixty stations in France, distributed accross five major catchment areas. The relationships between six of the variables: dissolved oxygen, BOD₅, ammonia nitrogen, nitrites, Kjeldhal nitrogen and orthophosphates can be considered as indicators of biodegradable pollution and the populations of forty eight species of molluscs have been studied using correspondence analysis. A scale of sensitivity of these to this type of pollution is presented.     

Ecological studies on reovirus pollution of rivers in Toyama Prefecture

Reoviruses (reos) were isolated from river water in various areas of Toyama Prefecture. The frequency of reo isolation was higher in the river water, the basin of which has a larger human population. The degree of river contamination with reo paralleled that with the Escherichia coli group of organisms, and reos were frequently isolated from sewage, too. The high antibody-positive (greater than or equal to 1:8 or greater than or equal to 1:10) rates against reos in humans and other animals tested (swine, cattle, and field rodents) indicated their wide-spread infection with reos. These results suggested that the major source of reos present in the river water may be the excretion by humans and other animals, especially the former. Survival experiments in which reos were added into the filtered or centrifuged river water and kept at various temperatures, revealed that reos survived for more than 3 years at 5 C. In the field experiment where reos suspended in cellophane tubes were kept in an agricultural water stream in winter (water temperatures below 10 C), they survived for 6 months until the water temperature rose above 20 C in summer.     

Viral pollution of the rivers in Toyama City

Viral pollution of the river water in Toyama City was surveyed during the two-year period from July 1979 to July 1981, and the ecology of viruses in the river water is discussed. Virus isolation from the river water samples, or from the water squeezed from cotton pads that were immersed in the stream for 3 days, was carried out by the "filter adsorption/elution" method. River waters were found to be contaminated with various species of enteric viruses, that is, poliovirus, echovirus, coxsackievirus, adenovirus, and reovirus. Poliovirus was isolated during the period immediately after the oral administration of polio vaccine, and coxsackie B virus was frequently isolated all year around. The enterovirus concentration in the river water was significantly high with a maximum of five plaque-forming units of coxsackie B2 virus per 250 ml. The species and type distribution of enteroviruses isolated from the river water coincided well with that of viruses isolated from inhabitants of Toyama Prefecture, with the exception of reovirus which was the largest population of virus species in the river water.     

Extraction and physico-chemical characterization of a versatile biodegradable polysaccharide obtained from green algae

During the last years, considerable attention has been given to different marine organisms, like algae, as potential sources of valuable materials. The continuous demand for novel materials and technologies is high and research on the underexploited marine green algae, including its polysaccharidic part—ulvan, has increased accordingly. In this research work, a novel method for extraction of ulvan from green algae is proposed and demonstrated successfully. Different characterization techniques were employed to characterize the isolated algal polysaccharide, namely, on what concerns its thermal trace and crystallinity. Upon heating, ulvan behaves as a non-meltable polysaccharide that is thermally stable before degradation at 220 °C. Ulvan is semi-crystalline in nature and possesses high hygroscopic features, as revealed in this research work. Due to its properties, ulvan can be considered, pure or modified, as a versatile biodegradable polymer for different applications, including tissue engineering and regenerative medicine.     

Using diatom life-forms and ecological guilds to assess organic pollution and trophic level in rivers: a case study of rivers in south-eastern France

The European Union’s Water Framework Directive has set a target of achieving good ecological status for all aquatic environments in Europe by 2015. In order to determine the quality of aquatic environments, biological indicators such as diatoms are often used. However, biotic diatom indices can be difficult and time consuming to use because of complexity of species determination. We investigated whether the biological traits of diatoms in rivers (life-forms, size classes and ecological guilds) could be used to assess organic pollution and trophic level. We worked on a data set comprising 315 diatom species, determined at 328 river stations of south-east France and a variety of parameters. The abundances of some biological traits differed significantly between the different organic pollution and trophic levels, particularly stalked diatoms, and the motile and low-profile guilds.     

Comparative studies of skeletal soluble matrices from some Scleractinian corals and Molluscs

The soluble organic matrices extracted from aragonitic skeletons of five Scleractinia and two Molluscs were analyzed using FTIR spectrometry, HPLC and 2-D gel electrophoresis. All these methods show that scleractinian and molluscan matrices are different. Both matrices are acidic with poorly separated molecular weights. The scleractinian matrices are highly glycosylated, whereas the molluscan matrices are not as shown by stains in 2D-electrophoresis. These results were in accordance with earlier data: high S contents in Scleractinia skeletons and low S contents in Mollusca shells. These results confirm the control of the morphology and the chemical composition of aragonite biocrystals in various taxa.     

Assessing the ecological values of rivers: an application of a multi-criteria approach to rivers of the South Coast Region,Western Australia

There is a growing awareness that in the light of increased threats, Australia’s freshwater ecosystems of highest value need to be protected. This implies that a consistent and comprehensive system for the classification of freshwater systems in terms of their ecological values is required for conservation planning. Despite earlier steps taken to develop appropriate methodologies for prioritising Australian waterways for protection, there is, as yet, no nationally agreed method for prioritising waterways in Australia based on their ecological values. Using the core criteria of naturalness, representativeness, diversity, rarity and special features, this paper describes the development of a framework of criteria, indicators and measures for the identification of high conservation value aquatic ecosystems, and trials this system by undertaking a comparative assessment of the ecological values of river systems in the South Coast region, Western Australia. A total of 33 river systems located in two aquatic bioregions were successfully ranked according to their ecological value, confirming that the development and implementation of a relatively simple system for assessing ecological values of Australian rivers shows promise, and that core criteria identified previously in the literature provide a good starting point for assessing ecological values of Australian aquatic ecosystems.     

Lack of oxygen and low pH as limiting factors for Gammarus in Hessian brooks and rivers

During two decades (1968–1988) the occurrence of Gammarus was studied in Hessian running waters draining some 7700 km² of the Fulda-Eder-basin and two adjacent areas. About 1530 sites were sampled, most of them in 1968, 1978, 1981/82 and 1985, which made comparisons possible, primarily with respect to space, but partly also to time. After consideration of the relief of the landscape, geological conditions, and water-quality in the neighbourhood of human settlements, lack of oxygen (due to organic pollution) and low pH (souring) can be recognized as the most important factors which influence and alter natural distribution patterns of Gammarus fossarum, G. pulex and G. roeseli. Both vanishing and recovering populations were observed in the course of twenty years.     

The relationship between alien crustaceans and pollution in Croatian large rivers: implications for biological monitoring

Hydrobiologia - Peracarid crustaceans contribute to the homogenization of macroinvertebrate communities as one of the most important group of aquatic invaders. This study investigated the...     

Development and practical application of limiting values for the phosphate concentration in surface waters in the Netherlands

Summary To counteract eutrophication it is important to possess sufficient information (1) on the relationship between the content of nutrients and the algal biomass, and (2) on the cycling of nutrients in lakes.A comparative study of a large number of surface waters in the Netherlands has demonstrated that it is possible to derive a relationship between the nitrogen and phosphorus concentrations and the upper limit of the chlorophyll concentration, averaged over the summer season.For the authority in charge of water quality it is essential to know how far the phosphorus loading must be reduced to attain the desired phosphate concentration. The results of an extensive study of the nutrient budgets of Lake Wolderwijd-Nuldernauw over the period 1976–1979 demonstrate that in certain cases a relative high phosphate concentration can occur (approx. 0.30 mg/l) in spite of a low external phosphorus loading (approx. 0.7 g P/m² year). Such high concentrations can be explained by the continuous presence of a bloom of blue-green algae and the release of phosphate from the aquatic sediments during the summer. In such lakes it is necessary to take additional measures, such as flushing with water poor in algae and phosphates, or, where necessary, the removal of aquatic sediment rich in phosphate.     

Matrix Proteins in the Outer Shells of Molluscs

The shells of molluscs are composed mainly of calcium carbonate crystals, with small amounts of matrix proteins. For more than 50 years, they have attracted attention for their unique mechanical and biological properties. Only recently, however, have researchers begun to realize that it is the matrix proteins that control the formation of calcium carbonate crystals and play key roles in their extraordinary properties, despite the fact that matrix proteins comprise less than 5% of the shell weight. This article reviews the matrix proteins identified to date from the shells of molluscs, their structural characteristics, and their roles in shell formation. Some suggestions are given for further investigation based on the summary and analysis.     

Introduction to the studies of pollution in the Oslofjord

Summary 1. The Oslofjord penetrates inland over a distance of about 60 nautical miles from the open Skager-Rak to the capital city of Norway. At the town of Drøbak, about 2/3 up the fjord, there are a series of obstacles to a free exchange of water, the main passage being a channel about 300 m wide with a sill depth of 19 m. Inside Drøbak, the fjord consists of two wider basins, Vestfjorden and Bunnefjorden with depths down to 164 and 160 m. The area of the inner fjord is 191 km²; it holds about 9.4×10⁹ m³ of water. The discharge of freshwater is about 800 million m³ per year, polluted by a population of 700 thousand and considerable industry.2. Faunistic studies were made byO. F. Müller in the 18th, M. andG. O Sars in the 19th century, while more extensive hydrobiological studies were initiated byJ. Hjort andH. H. Gran about 1900. They were subsequently extended through participation of the staffs of biological institutes of the University of Oslo, especially from the early thirties, when the importance of the pollution for the biology of the inner fjord had become well documented.3. In 1953 the situation had become so alarming that the authorities of the city of Oslo were warned that the use of the fjord as recipient for the city sewage had detrimental effects which required their attention and that more comprehensive studies were needed. The Norwegian Institute of Water Research (NIVA) was asked to plan and direct the cooperative investigations which have been carried out in the years 1962 to 1965. The Institute of Marine Biology of the University of Oslo and the Geophysical Institute of the University of Bergen participated in these studies.4. This summer the results were reported to the supporting authorities in a general report, edited byK. Baalsrud, Director of NIVA, and in 20 special reports by participating scientists.

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Einführung in die Untersuchungen über die Verunreinigung im Oslofjord

Kurzfassung Der Oslofjord erstreckt sich etwa 100 km in nördlicher Richtung vom Skagerrak bis nach Oslo. Bei Drøbak, etwa auf dem zweiten Drittel des Weges nach Oslo, gibt es eine Reihe von Hindernissen, die einem ungehinderten Austausch der Wassermassen im Fjord entgegenstehen. Der wichtigste Durchlaß ist nur etwa 300 m breit und hat eine Wassertiefe über der Schwelle von 19 m. Hinter Drøbak besteht der Fjord aus zwei Becken, dem Westfjord und dem Bunnefjord, die Tiefen von 164 m beziehungsweise von 160 m aufweisen. Der Fjord hinter Drøbak hat eine Fläche von 191 km²; die Wassermenge beträgt nach Berechnungen 9,4 Milliarden m³. Der jährliche Zufluß an Süßwasser, das durch Abwässer von ca. 700 000 Menschen und einer bedeutenden Industrie verunreinigt wird, beträgt etwa 800 Millionen m³. Studien über die Fauna wurden im 18. Jahrhundert vonO. F. Müller und im 19. Jahrhundert von M. undG. O. Sars vorgenommen. Ausgedehntere Untersuchungen wurden um 1900 vonJ. Hjort undH. H. Gran begonnen. Seit Anfang der dreißiger Jahre sind diese Untersuchungen von den biologischen Instituten der Universität Oslo weitergeführt worden. 1953 wurde die Situation hinsichtlich der Verunreinigung des Fjords alarmierend. Die Behörden der Stadt Oslo wurden davon unterrichtet, daß umfassendere Untersuchungen notwendig seien, um die Folgen, die dadurch entstehen, daß der Fjord als Auffangbecken für die Abwässer der Stadt dient, zu erforschen. Das norwegische Institut für Wasserforschung NIVA (Norsk institutt for vannforskning), wurde gebeten, diese Untersuchungen vorzubereiten und zu leiten. Im Juni 1967 konnte das Institut seinen allgemeinen Bericht sowie 20 Spezialberichte über dieses Forschungsprojekt, das in den Jahren 1962 bis 1965 bearbeitet worden war, vorlegen.

     

The macroinvertebrate communities of two contrasting Norwegian glacial rivers in relation to environmental variables

1. Macroinvertebrate communities in two Norwegian glacial rivers, one in the western fjords (Dalelva) and one in the eastern mountains (Leirungsåi), were investigated during three time periods in 1996 and 1997.
2. Channel stability variables (substratum heterogeneity/Pfankuch index/hydraulic stress) and water temperature accounted for 54% of the total inertia in the principal components analysis (PCA) ordination of environmental variables. The importance of these variables was confirmed by cluster analysis.
3. The two rivers were well separated in the ordinations, with Leirungsåi showing much greater heterogeneity. This is explained by differences in altitudinal range, terrestrial vegetation and the importance and nature of tributary inputs.
4. Channel stability and temperature were also important in determining faunal communities in the two glacial rivers, supporting the main determining variables in the conceptual model of glacial streams ( Milner & Petts, 1994 ). However, clear temporal differences were apparent in the data, the two rivers being more similar during the summer period of high discharge dominated by glacial meltwater. During spring and especially during autumn environmental conditions and the macroinvertebrate fauna differed both within and between rivers.
5. Diamesinae dominated in the upper reaches of both rivers, with Orthocladiinae becoming more common downstream. The dominance of Diamesinae persisted further down Dalelva because of the continued influence of glacial tributaries, whereas in Leirungsåi the influence of non-glacial tributaries led to a change towards a greater proportion of Orthocladiinae. Lakes modified macroinvertebrate communities in both river systems.     

Disentangling the roles of spatial and environmental variables in shaping benthic algal assemblages in rivers of central and northern China

Benthic algae were collected from central and northern Chinese rivers to test the hypothesis that geographic location has significant contributions in shaping algal assemblages. We used Moran’s eigenvector maps (MEM) to model spatial components and variation partitioning to quantify the influences of spatial and environmental variables on regional patterns of algal richness and community composition, respectively. We found that variation in algal richness was attributed to MEM component 2, 8, and 9 and the quadratic term of N–NO₃. Regarding abundance data, latitude, longitude, and MEM component 1, 2, and 7 were important spatial variables. Although P–PO₄, pH, and annual mean temperature were significant environmental variables influencing algal community composition, they were all spatially structured. Among the total explained variance in both algal metrics, spatial proportions were higher than that of environmental variables. We also found that abundant species of Achnanthidium minutissimum, Cocconeis placentula, Cymbella delicatula, Cymbella affinis, Cymbella turgidula, and Synedra ulna displayed clear spatially related patterns. In conclusion, the contributions of spatial and environmental variables to regional variation of algal assemblages are scale-dependent. As for our study scale (~1,000 km), spatial control may be more important. Since spatial effects could obscure local environmental impacts on algal communities, appropriate study scale and statistical methods should be taken into account in algal bioassessment. We recommend inclusion of both algal richness and community composition in study of algal biogeography, due to their different relationships with spatial and environmental variables.     

The rate limiting step in the reticulocyte uptake of transferin and transferrin iron Effects of some incubation variables

Reticulocytes incubated in an isotonic NaCl saline medium containing glucose, glutamine and amino acids, were able to detach both iron atoms from all the transferrin incorporated by them. In the absence of these metabolites, although transferrin uptake was the same, the reticuloctes failed to remove completely the iron from the transferrin which they incorporated.It has been shown before that there is unspecific as well as specific binding of transferrin to the reticulocyte. By incubating the cells in the presence of a high concentration of bovine serum albumin, we have been able to prevent the unspecific attachment of transferrin.At least 94% of the iodinated transferrin was capable of donating its iron to the reticulocytes.     

Optimization of cationic lipid mediated gene transfer: structure-function,physico-chemical,and cellular studies

The rationale design aimed at the enhancement of cationic lipid mediated gene transfer is discussed. These improvements are based on the straight evaluation of the structure-activity relationship and on the introduction of new structures. Much attention have been given to the supramolecular structures of the lipid/DNA complexes, to the effect of serum on gene transfer and to the intracellular trafficking of the lipoplexes. Finally new avenue using reducible cationic lipids has been discussed.     

Rapid assessment of rivers using macroinvertebrates: Case studies in the Nepean River and Blue Mountains,NSW

Abstract Two sets of data were used to evaluate the procedure for rapid assessment of rivers described by Chessman (1995): (i) 72 samples from four habitats at 27 sites on the Nepean River and tributaries; and (ii) 41 riffle samples from the Blue Mountains. In the Nepean system all the sites had moderate anthropogenic disturbance but none had gross organic pollution. There were, however, conductivity differences related to mixed sandstone and shale lithology. The sites differed widely in natural physical attributes such as stream order (2–6) and altitude (40–600 m). The Blue Mountains data included sites with little or no anthropogenic disturbance and sites greatly affected by organic pollution. There was again a wide range of altitudes (15–1000 m) but stream orders were more restricted (1–4). The occurrence of macroinvertebrate families was analysed using cluster analysis (TWINSPAN) and ordination (semi-strong hybrid multidimensional scaling). Relationships between the patterns observed, the SIGNAL biotic index, and physical and chemical data were investigated. The Nepean data showed that the sample size of 100 animals used in the rapid procedure was sufficient to reveal natural distribution patterns in the communities, and that SIGNAL was essentially independent of these patterns. In the Blue Mountains, water pollution had a greater effect on macroinvertebrate communities than the physical habitat, and SIGNAL distinguished sites with differing levels of pollution.     

Peripheral Actions of the SCPs in Aplysia and Other Gastropod Molluscs

The SCPs are a family of neuropeptides found in many gastropodspecies. Two SCPs with similar sequences have been characterizedin Aplysia. These peptides are potent modulators of centraland peripheral synapses. They also enhance ongoing contractileactivity in spontaneously active tissues such as heart and gut.Their distribution in central ganglia suggests that their predominantrole is in the regulation of feeding behavior. There is goodevidence that the identified SCP-containing neurons, B1 andB2, provide the major central regulation of gut motility duringfeeding through the release of the SCPs from their terminalsin gut. The SCPs have also been localized to motor neurons thatinnervate buccal muscles which generate biting and swallowingmovements. In many of these neurons, the SCPs have been shownto coexist with conventional transmitters such as ACh, or otherpeptides such as FMRFamide. The SCPs appear to be released alongwith conventional transmitters from these neurons to modulatethe effectiveness of the conventional transmitter. In all cases,the SCPs cause an enhancement of the amplitude of contractionsproduced by motor neuron stimulation. The precise mechanismsunderlying this effect vary from muscle to muscle. All of theeffects of the SCPs are mediated by increased cAMP levels intarget tissue. At many sites of action, serotonin produces actionsthat are qualitatively similar to those of the SCPs. This islikely to involve a convergence at the level of the adenylylcyclase. In addition to these peripheral effects, the SCPs alsohave multiple central effects on feeding and other behaviorsin gastropods.