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1.
Abscisic Acid Production and Water Relations in Wilty Tomato Mutants Subjected to Water Deficiency 总被引:7,自引:3,他引:4
Neill, S. J. and Horgan, R. 1985. Abscisic acid production andwater relations in wilty tomato mutants subjected to water deficiency.J.exp. BoL 36: 1222-1231. Abscisic acid (ABA) concentrations were determined in shootsof Lycopersicon esculentum Mill. cv. Ailsa Craig wild type andthe three wilty mutants notabilis (not), flacca (flc) and sitiens(sit). ABA content of unstressed wild type leaves was 1.5 nmolg1 fr. wt.; concentrations in not, flc and sit were 49,26 and 15% of this respectively. Gradual water stress was imposed on potted plants and a morerapid stress imposed on detached leaves. Leaves of the wildtype and not responded to both stresses by increasing theirABA content but leaves of flc and sit did not produce any moreABA under stress. Transpiration rates of flc plants were three times greater thanthose of the wild type and stomatal resistances correspondinglylower. Stomata of both flc and the wild type responded to darknessand externally supplied ABA by closing. However, only wild typestomata responded to water stress by dosing; those of flc leavesremained open until the leaves were severely desiccated. Thus,there was some relationship between the lack of stomatal responseto water stress and the failure to synthesize ABA. Key words: ABA, biosynthesis, stomata, water shortage, wilty mutants 相似文献
2.
Xanthoxin Metabolism in Cell-free Preparations from Wild Type and Wilty Mutants of Tomato 总被引:9,自引:5,他引:4 下载免费PDF全文
Extracts prepared from the turgid and water-stressed leaves of wild-type tomato (Lycopersicon esculentum Mill cv Ailsa Craig) and the wilty mutants sitiens, notabilis, and flacca were tested for their ability to metabolize xanthoxin to ABA. Extracts from wild type and notabilis converted xanthoxin at similar rates, while extracts from sitiens and flacca showed little or no activity. We also observed no activity when extracts of sitiens and flacca were mixed. Similar results were obtained when ABA aldehyde was used as a substrate, in that extracts from wild type and notabilis were equally active, but extracts from flacca and sitiens showed little activity. None of the tomato extracts showed significant activity with xanthoxin acid, xanthoxin alcohol, or ABA-1′,4-′Trans-diol as substrates. Extracts from bean leaves (Phaseolus vulgaris L. cv Blue Lake) were similar to the wild-type tomato extracts in their ability to convert the various substrates to ABA, although excised bean leaves did convert ABA-1′,4′-trans-diol and xanthoxin alcohol to ABA when these substances were taken up through the petiole. These results are consistent with a role for xanthoxin as a normal intermediate on the ABA biosynthetic pathway, and they suggest that ABA aldehyde is the final ABA precursor. 相似文献
3.
Growth and Water Relations of Wilty Mutants of Tomato (Lycopersicon esculentum Mill.) 总被引:2,自引:0,他引:2
In contrast to some previous reports on the growth of the ABA-deficientwilty mutants of tomato, growth was at least as rapid in themutants as in the wild type, as long as an adequate plant waterstatus was maintained by growing the plants under mist. Moreover,shoot extension was greater and the rate of leaf productionmore rapid in the mutants. Stomatal changes in response to environmentand to time in the light-dark cycle were generally similar inboth wilty mutants and the wild type, though the wild-type weregenerally more closed. Grafting experiments confirmed that thegenotype of the shoot was dominant in determining stomatal aperture,though wild-type rootstocks could cause a slight reduction inthe stomatal conductance of mutant leaves. The effect on plantwater relations of draughting only part of the root system wasinvestigated in a split-root experiment. Withholdingwater from only part of the root system was found to lower significantlythe mean leaf water potential, even though the potential evaporationrate was kept very small. Key words: Abscisic acid, stomata, tomato 相似文献
4.
Electric Signaling and Pin2 Gene
Expression on Different Abiotic Stimuli Depend on a Distinct Threshold
Level of Endogenous Abscisic Acid in Several Abscisic Acid-Deficient
Tomato Mutants 下载免费PDF全文
Oliver Herde Hugo Pe?a Cortés Claus Wasternack Lothar Willmitzer Joachim Fisahn 《Plant physiology》1999,119(1):213-218
Experiments were performed on three
abscisic acid (ABA)-deficient tomato (Lycopersicon
esculentum Mill.) mutants, notabilis,
flacca, and sitiens, to investigate the
role of ABA and jasmonic acid (JA) in the generation of electrical
signals and Pin2 (proteinase
inhibitor II) gene expression. We selected
these mutants because they contain different levels of endogenous ABA.
ABA levels in the mutant sitiens were reduced to 8% of
the wild type, in notabilis they were reduced to 47%,
and in flacca they were reduced to 21%. In wild-type
and notabilis tomato plants the induction of
Pin2 gene expression could be elicited by heat
treatment, current application, or mechanical wounding. In
flacca and sitiens only heat stimulation
induced Pin2 gene expression. JA levels in
flacca and sitiens plants also
accumulated strongly upon heat stimulation but not upon mechanical
wounding or current application. Characteristic electrical signals
evolved in the wild type and in the notabilis and
flacca mutants consisting of a fast action potential and
a slow variation potential. However, in sitiens only
heat evoked electrical signals; mechanical wounding and current
application did not change the membrane potential. In addition,
exogenous application of ABA to wild-type tomato plants induced
transient changes in membrane potentials, indicating the involvement of
ABA in the generation of electrical signals. Our data strongly suggest
the presence of a minimum threshold value of ABA within the plant that
is essential for the early events in electrical signaling and mediation
of Pin2 gene expression upon wounding. In contrast,
heat-induced Pin2 gene expression and membrane potential
changes were not dependent on the ABA level but, rather, on the
accumulation of JA.The plant hormones ABA and JA play a predominant role in the
conversion of environmental signals into changes in plant gene
expression. An increase in endogenous ABA and JA levels precedes and is
involved in Pin2 (proteinase
inhibitor II) gene expression upon wounding
(Peña-Cortés et al., 1989, 1991, 1995, 1996; Farmer and
Ryan, 1992; Farmer et al., 1992). This increase in ABA and JA is not
restricted to the tissue damaged directly but can also be detected in
the nonwounded, systemically induced tissue
(Peña-Cortés et al., 1989; Peña-Cortés and
Willmitzer, 1995). The accumulation of ABA and JA have been described
for several plant species, including potato, tomato, and tobacco
(Sanchez-Serrano et al., 1991; Peña-Cortés and Willmitzer,
1995).Further evidence for the involvement of ABA and JA in wound-induced
Pin2 gene expression was provided by a series of experiments
in which potato plants were sprayed with ABA or JA and Pin2
mRNA accumulated in the absence of any wounding
(Peña-Cortés et al., 1989; Hildmann et al., 1992). Both
nonsprayed leaves and leaves that were sprayed directly showed
increased Pin2 mRNA levels with a pattern identical to the
one described for wounded plants (Peña-Cortés et al., 1988;
Peña-Cortés and Willmitzer, 1995). Conclusive evidence for
the involvement of ABA in wound-induced Pin2 activation was
obtained from mutants impaired in ABA biosynthesis. Consequently, wound
induction of Pin2 was not observed in the droopy
mutant of potato or the sitiens mutant of tomato
(Peña-Cortés et al., 1989). However, in these mutants
treatment with ABA caused a return of the accumulation of
Pin2 mRNA to levels normally found in wild-type plants upon
wounding (Peña-Cortés et al., 1991).Like wounding, the application of electrical current was able to
initiate ABA and JA accumulation in wild-type plants but not in
ABA-deficient plants (Herde et al., 1996). These results suggested
that, like wounding, electrical current requires the presence of ABA
for the induction of Pin2 gene expression (Herde et al.,
1996). In contrast to wounding and electrical current, burning of
leaves activated Pin2 gene expression in sitiens
mutants by directly triggering the biosynthesis of JA via an
alternative pathway that is independent of endogenous ABA levels (Herde
et al., 1996).To determine the endogenous levels of ABA that are sufficient to
mediate electrical current-, heat-, and wound- induced Pin2
gene expression via electrical signals, we used several tomato mutants
containing progressively reduced levels of ABA. The effects of these
attenuated ABA levels on JA content and membrane potentials and the
expression pattern of Pin2 genes were analyzed. Analysis of
JA content was conducted to confirm the existence of an alternative
pathway that is independent of endogenous ABA levels in the different
ABA-deficient mutants. 相似文献
5.
Neill, S. J., McGaw, B. A. and Horgan, R. 1986. Ethylene and1-aminocyclopropane-l-carboxylic acid production in flacca,a wilty mutant of tomato, subjected to water deficiency andpretreatment with abscisic acid J. exp. Bot. 37: 535541. Plants of Lycoperstcon esculentum Mill. cv. Ailsa Craig wildtype and flacca (flc) were sprayed daily with H2O or 2?102mol m3 abscisic acid (ABA). ABA treatment effected apartial phenotypic reversion of flc shoots; leaf areas wereincreased and transpiration rates decreased. Leaf expansionof wild type shoots was inhibited by ABA. Indoleacetic acid (IAA), ABA and l-aminocyclopropane-l-carboxylicacid (ACC) concentrations were determined by combined gas chromatography-massspectrometry using deuterium-labelled internal standards ABAtreatment for 30 d resulted in greatly elevated internal ABAlevels, increasing from 1?0 to 4?3 and from 0?45 to 4?9 nmolg1 fr. wt. in wild type and flc leaves respectively.Endogenous IAA and ACC concentrations were much lower than thoseof ABA. IAA content ranged from 0?05 to 0?1 nmol g1 andACC content from 0?07 to 0?24 nmol g1 Ethylene emanationrates were similar for wild type and flc shoots. Wilting of detached leaves induced a substantial increase inethylene and ACC accumulation in all plants, regardless of treatmentor type. Ethylene and ACC levels were no greater in flc leavescompared to the wild type. ABA pretreatment did not preventthe wilting-induced increase in ACC and ethylene synthesis. Key words: ABA, ACC, ethylene, wilting, wilty mutants 相似文献
6.
WANG TREVOR L.; DONKIN MARIA E.; MARTIN E. STEPHEN 《Journal of experimental botany》1984,35(8):1222-1232
Wilty (JI 1069), a mutant of Pisum sativum, hasbeen examined for its ability to produce abscisic acid (ABA)under water stress. ABA was measured using combined gas chromatography-massspectrometry and multiple-ion-monitoring employing a deuteratedinternal standard. In intact droughted plants, Wiltyproduced less ABA than a non-wilty line (JI 1194) and maximumproduction was delayed. Detached leaves of the wilty mutantlost significantly more water than control leaves but did notshow an increase in ABA content. Non-stressed mutant materialfrom both intact plants and isolated leaves contained less ABAthan control tissue. Key words: Pea, Wilty mutant, Abscisic acid 相似文献
7.
Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: V. Effect of Abscisic Acid on Indoleacetic Acid Metabolism and Ethylene Evolution 总被引:4,自引:2,他引:2 下载免费PDF全文
Tal M 《Plant physiology》1979,63(6):1044-1048
The wilty tomato mutant flacca, the normal cultivar Lycopersicon esculentum Mill. Rheinlands Ruhm, and abscisic acid-induced phenotypic revertants were compared with respect to ethylene evolution, activity of tryptophan aminotransferase, and [1-14C]indoleacetic acid decarboxylation. 相似文献
8.
Analyses of abscisic acid, phaseic acid and dihydrophaseic acidby high performance liquid chromatography were carried out onto-2 tomato mutant plant, which has strong apical dominanceexpression. Significant differences in comparison to the normalplant were found only at 20 days after sowing. Of particularinterest for the to-2 phenotype trait was the high quantitiesof these substances in the roots of this mutant. (Received April 20, 1984; Accepted November 21, 1984) 相似文献
9.
Shirley M. Norman Stephen M. Poling V. P. Maier Mary D. Nelson 《Bioscience, biotechnology, and biochemistry》2013,77(8):2317-2324
Several compounds having the basic α-ionylideneacetic acid structure were tested in Cercospora rosicola resuspensions. At 100 μm, all the compounds inhibited abscisic acid (ABA) biosynthesis. Time studies with unlabelled and deuterated (2Z,4E)- and (2E,4E)-α-ionylideneacetic acids showed rapid conversions into both (2Z,4E)- and (2E,4E)-4′-keto-α-ionylideneacetic acids as major products. Incorporation of the label into ABA was specific for the 2Z,4E-isomer. Minor products, identified by GC-MS, were (2Z,4E)- and (2E,4E)-4′-hydroxy-α-ionylideneacetic acids and (2Z,4E)-1′-hydroxy-α-ionylideneacetic acid. The conversion to (2Z,4E)-l′-hydroxy-α-ionylideneacetic acid has not been previously reported and was specific for the 2Z,4E-isomer. A time study for the conversion of methyl esters of [2H3]-(2Z,4E)- and [2H3]-(2E,4E)-4′-keto-α-ionylideneacetates showed a slow introduction of the l′-hydroxyl group and specificity for 2Z,4E-isomer. Conversion of the ethyl esters of (2Z,4E)- and (2E,4E)-l′-hydroxy-α-ionylideneacetates into the ethyl esters of both ABA and (2E,4E)-ABA demonstrated that ABA can be formed by oxidation of the 4′-position after the insertion of the 1′-hydroxy group. The ethyl 1′-hydroxy acids were also isomerized to the corresponding ethyl (2Z,4E)- and ethyl (2E,4E)-3′-hydroxy-β-ionylideneacetates. Ethyl (2Z,4E)-1′-hydroxy acid also gave small amounts of ethyl l′,4′-trans-diol of ABA. These results suggest that ABA may be formed through a (2Z,4E)-1′-hydroxy-α-ionylidene-type intermediate in addition to the previously proposed route through (2Z,4E)-4′-keto-α-ionylideneacetic acid. 相似文献
10.
Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: II. Auxin- and Abscisic Acid-like Activity 总被引:9,自引:8,他引:1 下载免费PDF全文
The wilty tomato mutant, flacca, and the control variety, Rheinlands Ruhm, were compared with regard to the endogenous activity and concentration of auxin- and abscisic acid-like substances during ontogeny. The mutant wilts fast under water deficit because of inability to close its stomata. Symptoms characteristic of excessive auxin are evident in the developing mutant. Among these symptoms are branch and leaf epinasty, excessive rooting along the stem, and increased apical dominance. By using a leucine-incorporation assay, spray of whole plants with 2,4-dichlorophenoxyacetic acid, and wheat coleoptile bioassay, indications were found of an excess of activity and concentration of auxin-like substances in shoots of young and mature mutant plants. The wheat coleoptile bioassay also revealed a much lower amount of substances with abscisic acid-like activity in the mutant compared with the normal plant. In contrast to the appearance during ontogeny of morphological symptoms characteristic of auxin excess in the mutant, the absolute amount of auxin-like substances and their activity in incorporation of leucine decreased with age. A parallel decrease of the concentration and activity of auxin-like compounds was also found in the normal plant. The concentration of abscisic acid-like substances increased with age in both genotypes. The disagreement between the increasing morphological symptoms and the decrease of auxin-like activity and concentration is discussed, together with the possibility of a causal relationship between auxin-and abscisic acid-like activity and stomatal behavior. 相似文献
11.
12.
Homogenates of tomato (Lycopersicon esculentum Mill.) tissues oxidize IAA at a rate proportional to their peroxidase activity. Moreover, isoproteins with peroxidase activity catalyze also the oxidation of IAA. Two monogenic recessive mutations, olivacea and monstrosa, are involved in the control of the peroxidase-IAA oxidase activity. In olivacea leaflets this activity is 15 times that of the normal; all the aerial organs of monstrosa show high activities, particularly the midrib. These high activities depend on the enhancement of the organ-specific isoenzymes and on the derepression of the root isoenzyme band C2. This latter peroxidase-IAA oxidase band is present in olivacea and monstrosa leaflets, in monstrosa stems and, less intensely stained, in monstrosa midribs. The reduction in length and weight of mutant organs and their epinastic and geotropic-like behavior are associated with high peroxidase-IAA oxidase activities and with the derepression of the root-specific band C2. These results suggest an important role of the peroxidase-IAA oxidase system in controlling the metric relationships among different organs of the plant. Olivacea and monstrosa are to be viewed as auxin-related mutations controlling the synthesis of a group of proteins related in function. 相似文献
13.
Abnormal Stomatal Behavior and Hormonal Imbalance in flacca, a Wilty Mutant of Tomato: IV. Effect of Abscisic Acid and Water Content on RNase Activity and RNA 总被引:2,自引:1,他引:1 下载免费PDF全文
Plants of the wilty tomato (Lycopersicum esculentum) mutant, flacca, and of the normal cultivar Rheinlands Ruhm growing under either “normal” or high humidity were used in this research. Under normal humidity, RNase activity was much higher in mutant plants in which abscisic acid (ABA) and water content were lower than in the normal plant. The mutant also contained less RNA and protein per cell and less soluble RNA relative to ribosomal RNA as compared with the normal genotype. In ABA-treated mutant plants, RNase activity decreased while RNA, protein, the ratio of soluble to ribosomal RNA and water content increased. 相似文献
14.
Water Relations and Growth of the flacca Tomato Mutant in Relation to Abscisic Acid 总被引:2,自引:1,他引:1 下载免费PDF全文
Bradford KJ 《Plant physiology》1983,72(1):251-255
The flacca mutant in tomato (Lycopersicon esculentum Mill. cv Rheinlands Ruhm) was employed to examine the effects of a relatively constant diurnal water stress on leaf growth and water relations. As the mutant is deficient in abscisic acid (ABA) and can be phenotypically reverted to the wild type by applications of the growth substance, inferences can be made concerning the involvement of ABA in responses to water stress. Water potential and turgor were lower in leaves of flacca than of Rheinlands Ruhm, and were increased by ABA treatment. ABA decreased transpiration rates by causing stomatal closure and also increased the hydraulic conductance of the sprayed plants. Osmotic adjustment did not occur in flacca plants despite the daily leaf water deficits. Stem elongation was inhibited by ABA, but leaf growth was promoted. It is concluded that, in some cases, ABA may promote leaf growth via its effect on leaf water balance. 相似文献
15.
Teruhiko Yoshihara Sadao Sakamura 《Bioscience, biotechnology, and biochemistry》2013,77(12):2427-2429
Two compounds were isolated from alfalfa. Their structures were determined as benzoyl meso-tartaric acid (I) and benzoyl (S), (–)-malic acid (II) respectively. They were found in nature for the first time. 相似文献
16.
L. Irina Zaharia Mary K. Walker-Simmon Carlos Nicolás Rodríguez Suzanne R. Abrams 《Journal of Plant Growth Regulation》2005,24(4):274-284
Recently there have been breakthroughs on a number of fronts in abscisic acid (ABA) biology research that have advanced the
field significantly, including discovery of genes involved in ABA metabolism, along with progress in understanding of ABA
signaling (Finkelstein and others 2002; Kushiro and others 2004; Lim and others 2005; Saito and others 2004). At the same time, the chemistry of ABA has advanced. New analytical methods have been developed for profiling ABA and catabolites
(Ross and others 2004; Zaharia and others 2005). Novel bioactive catabolites have been discovered from feeding studies with deuterated ABA and catabolites (Zaharia and others 2004; Zhou and others 2004). This review covers recent advances and prospects in natural products chemistry, analysis of ABA catabolism, and applications
of ABA analogs for biochemical studies and horticultural uses. 相似文献
17.
《Bioscience, biotechnology, and biochemistry》2013,77(12):2644-2650
A Chiralcel OJ column was used to determine the absolute configuration of naturally occurring α-ionylideneacetic acid from Cercospora rosicola and γ-ionylideneacetic acid from C. cruenta as (R) enantiomers in accordance with their biosynthetic product, (S)-ABA. Both enantiomers of [1, 2-13C2]-α and γ-ionylideneacetic acids were prepared and fed to C. rosicola and C. cruenta. Six combinations of feeding experiments comparatively and unequivocally demonstrated stereoselectivity in the biosynthetic conversions, including stepwise hydroxylation at C-1′ and 4′. Enzymatic isomerization from the γ to α-intermediate was suggested not to be involved in ABA biosynthesis in C. rosicola. 相似文献
18.
19.
植物ABA受体及其介导的信号转导通路 总被引:3,自引:0,他引:3
ABA是调控植物体生长发育和响应外界应激的重要植物激素之一。近年来, ABA受体的筛选和鉴定取得了突破性进展, 为植物中ABA信号转导通路的阐明奠定了重要基础。该文主要综述了ABA-binding protein/H subunit of Mgchelatase (ABAR/CHLH)、G protein-coupled receptor 2 (GCR2)、GPCR-type G protein 1/2 (GTG1/2)和pyrabactin resistant/PYR-like/regulatory component of ABA (PYR/PYL/RCAR)被报道为ABA受体的研究历程, 重点介绍了以ABAR/CHLH PYR/PYL/RCAR为受体的ABA信号转导通路模型的构建, 旨在为ABA受体及其信号转导通路的相关研究提供参考。 相似文献
20.
NaCI Reduces Indole-3-Acetic Acid Levels in the Roots of Tomato Plants Independent of Stress-Induced Abscisic Acid 下载免费PDF全文
Indole-3-acetic acid (IAA) was measured in leaves and roots of tomato (Lycopersicon esculentum) genotypes subjected to salt stress. An abscisic acid (ABA)-deficient mutant of tomato (sitiens), the genetic parent (Rheinlands Ruhm, RR), and a commercial variety (Large Cherry Red, LCR) of tomato were treated with 50 to 300 mM NaCl in nutrient culture. Both LCR and RR had significantly higher levels of IAA in the roots compared with that in sitiens prior to treatment. The initial levels of IAA in the roots of LCR and RR declined by nearly 75% after exposure to NaCl, whereas those in roots from the sitiens mutant remained unchanged. IAA levels in the leaves of all genotypes remained unchanged or increased slightly in response to NaCl. ABA was highest in leaves from the normal genotypes after exposure to NaCl. ABA levels in the roots of sitiens were similar to the levels in the normal genotypes, whereas levels in the leaves were only 10% of the levels found in normal genotypes regardless of the salt treatment. Treatment of LCR and sitiens with exogenous ABA increased the ABA levels in leaves and roots, but there were no measurable changes in endogenous IAA. Therefore, the reduction in IAA appears to result from an ABA-independent effect of NaCl on IAA metabolism in the roots of stressed plants. 相似文献