共查询到20条相似文献,搜索用时 0 毫秒
1.
Sterol regulatory element-binding protein-1 is regulated by glucose at the transcriptional level 总被引:10,自引:0,他引:10
Hasty AH Shimano H Yahagi N Amemiya-Kudo M Perrey S Yoshikawa T Osuga J Okazaki H Tamura Y Iizuka Y Shionoiri F Ohashi K Harada K Gotoda T Nagai R Ishibashi S Yamada N 《The Journal of biological chemistry》2000,275(40):31069-31077
2.
3.
4.
K Chakravarty P Leahy D Becard P Hakimi M Foretz P Ferre F Foufelle R W Hanson 《The Journal of biological chemistry》2001,276(37):34816-34823
5.
6.
7.
Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes 总被引:20,自引:0,他引:20
Shimano H Yahagi N Amemiya-Kudo M Hasty AH Osuga J Tamura Y Shionoiri F Iizuka Y Ohashi K Harada K Gotoda T Ishibashi S Yamada N 《The Journal of biological chemistry》1999,274(50):35832-35839
To elucidate the physiological role of sterol regulatory element-binding protein-1 (SREBP-1), the hepatic mRNA levels of genes encoding various lipogenic enzymes were estimated in SREBP-1 gene knockout mice after a fasting-refeeding treatment, which is an established dietary manipulation for the induction of lipogenic enzymes. In the fasted state, the mRNA levels of all lipogenic enzymes were consistently low in both wild-type and SREBP-1(-/-) mice. However, the absence of SREBP-1 severely impaired the marked induction of hepatic mRNAs of fatty acid synthetic genes, such as acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase, that was observed upon refeeding in the wild-type mice. Furthermore, the refeeding responses of other lipogenic enzymes, glycerol-3-phosphate acyltransferase, ATP citrate lyase, malic enzyme, glucose-6-phosphate dehydrogenase, and S14 mRNAs, were completely abolished in SREBP-1(-/-) mice. In contrast, mRNA levels for cholesterol biosynthetic genes were elevated in the refed SREBP-1(-/-) livers accompanied by an increase in nuclear SREBP-2 protein. When fed a high carbohydrate diet for 14 days, the mRNA levels for these lipogenic enzymes were also strikingly lower in SREBP-1(-/-) mice than those in wild-type mice. These data demonstrate that SREBP-1 plays a crucial role in the induction of lipogenesis but not cholesterol biosynthesis in liver when excess energy by carbohydrates is consumed. 相似文献
8.
9.
10.
Masaki Yoshida Nagakatsu Harada Tadahiko Nakagawa Kazuaki Mawatari Hiroshi Sakaue 《FEBS letters》2010,584(6):1217-1222
A link between cellular uptake of high density lipoprotein (HDL) and regulation of sterol regulatory element-binding protein-1 (SREBP-1) was investigated in vitro. HDL decreased nuclear SREBP-1 levels as well as SREBP-1 target gene expression in HepG2 and HEK293 cells. However, HDL did not repress an exogenously expressed, constitutively active form of SREBP-1. HDL increased cellular cholesterol levels, and cellular cholesterol depletion by methyl-β-cyclodextrin abolished the effects of HDL. These results suggest that HDL inhibits the activation of SREBP-1 through a cholesterol-dependent mechanism, which may play an important role in regulating lipid synthetic pathways mediated by SREBP-1. 相似文献
11.
12.
In vivo promoter analysis on refeeding response of hepatic sterol regulatory element-binding protein-1c expression 总被引:1,自引:0,他引:1
Takeuchi Y Yahagi N Nakagawa Y Matsuzaka T Shimizu R Sekiya M Iizuka Y Ohashi K Gotoda T Yamamoto M Nagai R Kadowaki T Yamada N Osuga J Shimano H 《Biochemical and biophysical research communications》2007,363(2):329-335
13.
14.
Melissa Gmez Sebastin Campusano María Soledad Gutirrez Dionisia Sepúlveda Salvador Barahona Marcelo Baeza Víctor Cifuentes Jennifer Alcaíno 《Journal of lipid research》2020,61(12):1658
Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces carotenoids, mainly astaxanthin. Astaxanthin is an organic pigment of commercial interest due to its antioxidant and coloring properties. X. dendrorhous has a functional SREBP pathway, and the Sre1 protein is the SREBP homolog in this yeast. However, how sterol regulatory element (Sre)1 promotes the biosynthesis of sterols and carotenoids in X. dendrorhous is unknown. In this work, comparative RNA-sequencing analysis between modified X. dendrorhous strains that have an active Sre1 protein and the WT was performed to identify Sre1-dependent genes. In addition, Sre1 direct target genes were identified through ChIP combined with lambda exonuclease digestion (ChIP-exo) assays. SRE motifs were detected in the promoter regions of several Sre1 direct target genes and were consistent with the SREs described in other yeast species. Sre1 directly regulates genes related to ergosterol biosynthesis as well as genes related to the mevalonate (MVA) pathway, which synthesizes the building blocks of isoprenoids, including carotenoids. Two carotenogenic genes, crtE and crtR, were also identified as Sre1 direct target genes. Thus, carotenogenesis in X. dendrorhous is regulated by Sre1 through the regulation of the MVA pathway and the regulation of the crtE and crtR genes. As the crtR gene encodes a cytochrome P450 reductase, Sre1 regulates pathways that include cytochrome P450 enzymes, such as the biosynthesis of carotenoids and sterols. These results demonstrate that Sre1 is a sterol master regulator that is conserved in X. dendrorhous. 相似文献
15.
16.
Titus MA Zeithaml B Kantor B Li X Haack K Moore DT Wilson EM Mohler JL Kafri T 《PloS one》2012,7(1):e30192
Background
Prostate cancer (CaP) is the second leading cause of cancer death in American men. Androgen deprivation therapy is initially effective in CaP treatment, but CaP recurs despite castrate levels of circulating androgen. Continued expression of the androgen receptor (AR) and its ligands has been linked to castration-recurrent CaP growth.Principal Finding
In this report, the ligand-dependent dominant-negative ARΔ142–337 (ARΔTR) was expressed in castration-recurrent CWR-R1 cell and tumor models to elucidate the role of AR signaling. Expression of ARΔTR decreased CWR-R1 tumor growth in the presence and absence of exogenous testosterone (T) and improved survival in the presence of exogenous T. There was evidence for negative selection of ARΔTR transgene in T-treated mice. Mass spectrometry revealed castration-recurrent CaP dihydrotestosterone (DHT) levels sufficient to activate AR and ARΔTR. In the absence of exogenous testosterone, CWR-R1-ARΔTR and control cells exhibited altered androgen profiles that implicated epithelial CaP cells as a source of intratumoral AR ligands.Conclusion
The study provides in vivo evidence that activation of AR signaling by intratumoral AR ligands is required for castration-recurrent CaP growth and that epithelial CaP cells produce sufficient active androgens for CaP recurrence during androgen deprivation therapy. Targeting intracrine T and DHT synthesis should provide a mechanism to inhibit AR and growth of castration-recurrent CaP. 相似文献17.
18.
Caveolin-1 interacts with androgen receptor. A positive modulator of androgen receptor mediated transactivation 总被引:10,自引:0,他引:10
Lu ML Schneider MC Zheng Y Zhang X Richie JP 《The Journal of biological chemistry》2001,276(16):13442-13451
19.