共查询到20条相似文献,搜索用时 31 毫秒
1.
Zdorovenko EL Varbanets LD Zatonsky GV Kachala VV Zdorovenko GM Shashkov AS Knirel YA 《Carbohydrate research》2008,343(14):2494-2497
The O-polysaccharide of Rahnella aquatilis 95 U003 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: 相似文献
2.
Kilcoyne M Perepelov AV Tomshich SV Komandrova NA Shashkov AS Romanenko LA Knirel YA Savage AV 《Carbohydrate research》2004,339(3):477-482
Mild acid degradation of the lipopolysaccharide of the bacterium Idiomarina zobellii, type strain KMM 231T, with aq 2% HOAc at 100 degrees C, yielded an oligosaccharide, which represents one repeating unit of the O-polysaccharide. A polysaccharide was obtained by mild base degradation of the lipopolysaccharide. The following structure of the O-polysaccharide was elucidated by 1H and 13C NMR spectroscopy of the oligosaccharide and base-degraded lipopolysaccharide, including COSY, TOCSY, ROESY, 1H, 13C HSQC, HSQC-TOCSY and HMBC experiments: [-->3)-alpha-D-Quip4N-(1-->4)-alpha-D-GlcpA-(1-->6)-alpha-D-GlcpNAc-(1-->4)-alpha-L-GulpNA-(1-->3)-beta-D-FucpNAc-(1-->] The O-polysaccharide is distinguished by the presence of two unusual amino sugars, 4-amino-4,6-dideoxy-D-glucose (D-Qui4N) and 2-amino-2-deoxy-L-guluronic acid (L-GulNA), both having the free amino group. The unexpectedly high acid lability of the glycosidic linkage of 2-acetamido-2,6-dideoxy-D-galactose (D-FucNAc) could be associated with the presence of a free amino group adjacent to the site of attachment of FucNAc to Qui4N. 相似文献
3.
Kocharova NA Ovchinnikova OG Toukach FV Torzewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2005,340(7):1419-1423
The O-polysaccharide (O-antigen) of Providencia stuartii O44:H4 (strain 3768/51) was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, and H-detected (1)H,(13)C HSQC, and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [Formula: see text]. 相似文献
4.
Perepelov AV Wang Q Senchenkova SN Shevelev SD Zhao G Shashkov AS Feng L Knirel YA Wang L 《Carbohydrate research》2006,341(12):2176-2180
A teichoic acid-like O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O29. The O-polysaccharide and an oligosaccharide obtained by dephosphorylation of the O-polysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy. The following structure of the branched oligosaccharide repeating unit, containing five monosaccharide residues and glycerol 1-phosphate (D-Gro-1-P), was established: [carbohydrate structure: see text]. 相似文献
5.
Kocharova NA Ovchinnikova OG Torzewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2006,341(6):786-790
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O30. Studies by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, ROESY, and H-detected (1)H,(13)C HSQC, HMBC, and HMQC-TOCSY experiments, showed that the polysaccharide has a linear pentasaccharide repeating unit of the following structure: 相似文献
6.
Ovchinnikova OG Bushmarinov IS Kocharova NA Toukach FV Wykrota M Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2007,342(8):1116-1121
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide from Providencia alcalifaciens O27 and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC, and HMBC experiments. It was found that the polysaccharide is built up of linear partially O-acetylated tetrasaccharide repeating units and has the following structure: [structure: see text] where Qui4NFo stands for 4-formamido-4,6-dideoxyglucose (4-formamido-4-deoxyquinovose). The O-polysaccharide structure of Providencia stuartii O43 established earlier was revised with respect to the configuration of the constituent 4-amino-4,6-dideoxyhexose (from Rha4N to Qui4N). 相似文献
7.
Ovchinnikova OG Kocharova NA Wykrota M Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2007,342(14):2144-2148
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O6 and studied by sugar and methylation analysis, selective hydrolytic removal of 3,6-dideoxy-L-xylo-hexose (colitose, Col), (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY and H-detected (1)H,(13)C HSQC and HMBC experiments. The polysaccharide was found to have a branched pentasaccharide repeating unit with the following structure: [see text] Remarkably, the trisaccharide side chain of the O6-polysaccharide represents a colitose ('3-deoxy-L-fucose') analogue of the H type 1 (precursor) antigenic determinant. 相似文献
8.
Ovchinnikova OG Kocharova NA Kondakova AN Bialczak-Kokot M Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2011,(16):2638-2641
An O-polysaccharide and oligosaccharides were isolated by GPC following mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O28. The O-polysaccharide was studied by sugar and methylation analyses, 1H and 13C NMR spectroscopy, including 2D ROESY and H-detected 1H,13C HSQC and HMBC experiments, and the following structure of the branched pentasaccharide repeating unit was established:This structure was confirmed by ESI MS of the isolated tridecasaccharide consisting of the lipopolysaccharide core and one O-polysaccharide repeat. The ESI mass spectrum also enabled inferring the composition of the core oligosaccharide. 相似文献
9.
Perepelov AV Han W Senchenkova SN Shevelev SD Shashkov AS Feng L Liu Y Knirel YA Wang L 《Carbohydrate research》2007,342(3-4):648-652
An acidic O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Escherichia coli O150 and studied by sugar and methylation analyses, triflic acid solvolysis, Smith degradation, (1)H and (13)C NMR spectroscopy, including 2D ROESY, (1)H,(13)C HSQC, HMQC-TOCSY, and HMBC experiments. The polysaccharide was found to contain a regioisomer of N-acetylisomuramic acid, 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose [d-GlcNAc4(Slac)]. The structure of its hexasaccharide repeating unit was established. 相似文献
10.
Kocharova NA Ovchinnikova OG Bushmarinov IS Toukach FV Torzewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2005,340(4):775-780
The O-polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O57:H29. Studies by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC, and HMBC experiments, showed that the polysaccharide contains an amide of D-galacturonic acid with L-alanine and has the following pentasaccharide repeating unit: [formula: see text] 相似文献
11.
Ovchinnikova OG Kocharova NA Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2012,347(1):168-171
An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O48 and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D COSY, TOCSY, ROESY and (1)H,(13)C HSQC and HMBC experiments. It was found that the polysaccharide is acidic and has a linear mono-O-acetylated tetrasaccharide repeating unit with the following structure: →3)-α-D-Manp-(1→2)-α-L-Fucp-(1→2)-β-D-GlcpA4Ac-(1→3)-α-D-GalpNAc-(1→. 相似文献
12.
Toukach FV Kocharova NA Maszewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2008,343(15):2706-2711
A glycerol teichoic acid-like O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O8 and studied by chemical methods and NMR spectroscopy, including 2D ROESY, {(1)H,(13)C} HSQC, and HMQC-TOCSY experiments. It was found that the compound contains a new component of bacterial lipopolysaccharides: ether-linked (2S,4R)-2,4-dihydroxypentanoic acid (Dhpa), which was identified by NMR spectroscopy. The following structure of the repeating unit of the polysaccharide was established: 相似文献
13.
Structural analysis of the three lipopolysaccharides produced by Salmonella madelia (1,6,14,25) 总被引:3,自引:0,他引:3
Salmonella madelia reported to express the O-antigenic factors 1, 6, 14, and 25, defined in the Kauffmann-White classification system, was found to produce three different homogeneous lipopolysaccharides, which differed in having three structurally distinct O-polysaccharide components. The O-polysaccharide fraction obtained by mild acetic acid hydrolysis of the S. madelia lipopolysaccharide was analyzed by chemical composition, nitrous acid deamination, periodate oxidation, methylation, and 1H and 13C nuclear magnetic resonance methods and was demonstrated to be composed of three polysaccharides, PS(I), PS(II), and PS(III), which had the structures of repeating oligosaccharide units: (formula; see text) 相似文献
14.
The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of Rahnella aquatilis 1-95 and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including NOESY and 1H,13C HSQC experiments for linkage and sequence analysis. The following structure of the branched trisaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text]. 相似文献
15.
Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O29 总被引:1,自引:0,他引:1
Bushmarinov IS Ovchinnikova OG Kocharova NA Toukach FV Torzewska A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2006,341(9):1181-1185
The O-polysaccharide was obtained by a mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O29. Structural studies were performed using sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including two-dimensional 1H, 1H COSY, TOCSY, ROESY, H-detected 1H, 13C HSQC and HMBC experiments. On the basis of the data obtained, the following structure of the branched tetrasaccharide repeating unit of the O-polysaccharide was established: [structure: see text]. 相似文献
16.
Perepelov AV Liu B Senchenkova SN Shashkov AS Feng L Knirel YA Wang L 《Carbohydrate research》2008,343(3):571-575
An acidic O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O112ab and studied by sugar analysis along with (1)H and (13)C NMR spectroscopy. The O-polysaccharide was found to contain a rarely occurring sugar component, L-iduronic acid (L-IdoA), and the following structure of the branched pentasaccharide repeating unit was established: [structure: see text]. 相似文献
17.
An acidic branched O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide (LPS) of Proteus genomospecies 4 and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, ROESY and H-detected 1H, 13C HSQC experiments. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established, which is unique among Proteus polysaccharide structures: [structure: see text] where Qui3NAc stands for 3-acetamido-3,6-dideoxyglucose. Based on the O-polysaccharide structure and serological data, we propose classifying Proteus genomospecies 4 into a new, separate Proteus serogroup, O56. A weak cross-reactivity of Proteus genomospecies 4 antiserum with LPS of Providencia stuartii O18 and Proteus vulgaris OX2 was observed and is discussed in view of a similarity of the O-polysaccharide structures. Structural and serological investigations showed that Proteus genomospecies 5 and 6 should be classified into the existing Proteus serogroups O8 and O69, respectively. 相似文献
18.
Ovchinnikova OG Kocharova NA Torzewska A Blaszczyk A Shashkov AS Knirel YA Rozalski A 《Carbohydrate research》2005,340(7):1407-1411
The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O43:H28 and studied by sugar and methylation analyses, Smith degradation and 1H and 13C NMR spectroscopy, including 2D ROESY, and H-detected 1H, 13C HSQC and HMBC experiments, as well as a NOESY experiment in a 9:1 H2O/D2O mixture to reveal correlations for NH protons. It was found that the polysaccharide is built up of linear tetrasaccharide repeating units containing an amide of D-galacturonic acid with L-serine [D-GalA6(L-Ser)] and has the following structure:[3)-beta-D-GalpA6(L-Ser)-(1-->3)-beta-D-GlcpNAc-(1-->2)-alpha-D-Rhap4NAc-(1-->4)-beta-D-GlcpA-(1-->]n. 相似文献
19.
Perepelov AV Liu B Senchenkova SN Shashkov AS Feng L Knirel YA Wang L 《Carbohydrate research》2007,342(17):2676-2681
The O-polysaccharide was isolated from the lipopolysaccharide of Escherichia coli O168 and studied by chemical analyses and Smith degradation along with (1)H and (13)C NMR spectroscopies. The following structure of the branched pentasaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text] where 6-O-acetylation of GlcNAc is partial. Reinvestigation of the O-polysaccharide of Shigella dysenteriae type 4 established earlier showed it to have the same structure except for that the lateral Fuc residue is nonstoichiometrically O-acetylated at each position. 相似文献
20.
Fedonenko YP Konnova ON Zatonsky GV Konnova SA Kocharova NA Zdorovenko EL Ignatov VV 《Carbohydrate research》2005,340(6):1259-1263
A neutral O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant-growth-promoting bacterium Azospirillum lipoferum Sp59b. On the basis of sugar and methylation analyses along with 1D and 2D (1)H and (13)C NMR spectroscopy, including a NOESY experiment, the following structure of the branched hexasaccharide repeating unit of the O-polysaccharide was established: [carbohydrate structure: see text]. 相似文献