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1.
Mangala D. Manandhar Aboo Shoeb Randhir S. Kapil Satya P. Popli 《Phytochemistry》1978,17(10):1814-1815
Four new alkaloids, O-(3,3-dimethylallyl)-halfordinol, N-2-ethoxy-2-(4-methoxyphenyl)ethylcinnamamide, N-2-methoxy-2-[4-(3′,3′-dimethyl 相似文献
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Techniques have been developed for the regeneration of Aegle marmelos from nucellar explants. Slow-growing calli were induced from nucellar explants excised from 90–120 d-old developing fruits. The medium consisted of Murashige and Skoog formulation containing 40 g/l sucrose, 400 mg/l casein hydrolysate, 5 mg/l 1-naphthaleneacetic acid and 1 mg/l kinetin. The basal medium with high concentration (1–5 mg/l) of N6-benzyladenine (BA) and low concentration (0.1 mg/l) of NAA was suitable for regeneration of shoots from 3-month-old calli. Addition of 1 mg/l gibberellic acid (GA3) favoured shoot growth. Callus-derived shoots produced roots and developed into plantlets when transferred to half-strength MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l NAA. Approximately 5 months were required for the full regenerative process. 相似文献
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《Phytochemistry》2012
Five (1–5) and 15 known compounds (6–20) were isolated from the acetone extract of the green fruits of Aegle marmelos. The structure of compounds 1–5, marmesiline (1), 6-(4-acetoxy-3-methyl-2-butenyl)-7-hydroxycoumarin (2), 6-(2-hydroxy-3-hydroxymethyl-3-butenyl)-7-hydroxycoumarin (3), marmelonine (4) and 8-hydroxysmyrindiol (5), were determined on the basis of spectroscopic analyses. Antifungal and antibacterial activities of selected compounds were also evaluated. 相似文献
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Bhuwan B. Mishra Desh D. Singh Navneet Kishore Vinod K. Tiwari Vyasji Tripathi 《Phytochemistry》2010,71(2-3):230-234
Antifungal constituents, 2-isopropenyl-4-methyl-1-oxa-cyclopenta[b]anthracene-5,10-dione and (+)-4-(2′-hydroxy-3′-methylbut-3′-enyloxy)-8H-[1,3]dioxolo[4,5-h]chromen-8-one in addition to known compounds imperatorin, β-sitosterol, plumbagin, 1-methyl-2-(3′-methyl-but-2′-enyloxy)-anthraquinone, β-sitosterol glucoside, stigmasterol, vanillin and salicin were isolated during phytochemical investigation on seeds of Aegle marmelos Correa. 相似文献
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M. Hossain M. R. Karim R. Islam O. I. Joarder 《Plant Cell, Tissue and Organ Culture》1993,34(2):199-203
A protocol for organogenesis from nucellar explants excised from fertilized ovules of immature fruits of Aegle marmelos Corr. was developed. Adventitious buds were initiated on Murashige and Skoog's (MS) medium containing various combinations of 6-benzyladenine (BA), -naphthalene-acetic acid (NAA), 3-indoleacetic acid and gibberellic acid. Medium containing 4.4 m BA and 2.7 M NAA produced the maximum number of adventitious buds per explant. Shoots were elongated by transferring explants with shoot buds to medium with a low concentration of BA (0.44 M). Rooting of in vitro-regenerated shoots was obtained in half-strength MS medium with 4.9 M indole-3-butyric acid. This is the first report of plant regeneration from nucellar explants of A. marmelos.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- NAA
naphthaleneacetic acid
- GA3
gibberellic acid 相似文献
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Following ion-exchange, Sephadex LH-20 and paper chromatography of the methanolic extracts of young, developing fruits of Aegle marmelos Correa, cytokinin-like activity in the soybean callus assay was detected in six fractions. Of the four butanol-soluble compounds, two were tentatively identified as zeatin and zeatin riboside, and the others as zeatin glucoside and zeatin riboside glucoside. The major cytokinino of the butanol-insoluble fraction is probably zeatin nucleotide. The levels of compounds with cytokinin-like activity were high during the early phase, and low in the subsequent period of fruit growth. The activity resembling that of cytokinin glucoside increased with maturation of the fruit. The content of free cytokinins in the fruits was more than that released from the tRNA. 相似文献
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《Phytomedicine》2013,21(14):1267-1271
In continuation of evaluating the anti-obesity effect of Aegle marmelos, we have screened the n-hexane, dichloro methane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts of the leaves at the concentration of 25, 50, 75 and 100 μg/ml for adipogenesis inhibition in the adipocytes. Nile red staining with the help of fluorometry was used as indicator of the antiobesity activity. The most active DCM extract showed the 33.98 ± 3.55% lipid content at 100 μg/ml and was selected for the further isolation. 14 compounds were isolated from DCM extract of A. marmelos leaves. The compounds were screened for the adipogenesis inhibition at 50 and 100 μM concentrations. Out of the 14 compounds, halfordinol, ethyl ether aegeline and esculetin were showing 10.04 ± 0.52, 16.29 ± 0.85 and 25.09 ± 1.31% lipid content respectively at 100 μM. We hereby report the adipogenesis inhibition by A. marmelos as one of the pathway for its antiobesity effect. 相似文献
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Yukiko Tokitomo Yūko Shimono Akio Kobayashi Tei Yamanishi 《Bioscience, biotechnology, and biochemistry》2013,77(7):1873-1877
The attractive and characteristically sweet aroma components of baelfruit—a tropical fruit— were investigated. The aroma concentrates possessing the sweet floral and somewhat terpene-like aroma were obtained from both the pulp and peel of fresh baelfruits by means of lyophilization and ether extraction, being analyzed mainly by GC-MS, A total of 39 components were identified. Among these components, terpene alcohols and β-βonone were considered to contribute to the aroma of baelfruit. At optimum ripeness, the fruit with excellent flavor contained a large quantity of an isomeric compound of 3,7-dimethyl-1,5,7-octatrien-3-ol. This compound couldn’t be found in unripe fruit, and seems to be ?mportant in making the baelfruit flavor attractive. 相似文献
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The aim of this study is to determine the affinity of six active compounds of Aegle Marmelos Correa, they are (E, R)-Marmin,
skimmianine, (S)-aegeline, aurapten, zeorin, and dustanin as antihistamines in histamine H1 receptor in comparison to cetirizin,
diphenhydramine and chlorpheniramine as ligands comparison. Previously, in the in vitro study marmin obviously antagonized
the histamine H1 receptor in a competitive manner. Methods: molecular docking to determine the interaction of ligand binding to its
receptor. Lower docking score indicates more stable binding to that protein. Results: Marmin, skimmianine, aegeline, aurapten,
zeorin, and dustanin were potential to develop as antihistamine agents, especially as histamine H1 receptor antagonists by
interacting with amino acid residues, Asp107, Lys179, Lys191, Asn198, and Trp428 of histamine H1 receptor. Conclusions: Based on
molecular docking, Amino acid residues involved in ligand protein interactions were Asp107, Lys179, Lys191, Asn198, and Trp428. 相似文献
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Jun Li Fakhri Mahdi Lin Du Mika B. Jekabsons Yu-Dong Zhou Dale G. Nagle 《Bioorganic & medicinal chemistry》2013,21(7):1795-1803
Bioassay-guided isolation and subsequent structure elucidation of a Bael tree Aegle marmelos lipid extract yielded two unstable acylated geranyloxycoumarin mixtures (1–2), six geranyloxycoumarins (3–8), (+)-9′-isovaleroxylariciresinol (9), and dehydromarmeline (10). In a T47D cell-based reporter assay, 1 and 2 potently inhibited hypoxia-induced HIF-1 activation (IC50 values 0.18 and 1.10 μg mL?1, respectively). Insufficient material and chemical instability prevented full delineation of the fatty acyl side chain olefin substitution patterns in 1 and 2. Therefore, five fatty acyl geranyloxycoumarin ester derivatives (11–15) were prepared from marmin (3) and commercial fatty acyl chlorides by semisynthesis. The unsaturated C-6′ linoleic acid ester derivative 14 that was structurally most similar to 1 and 2, inhibited HIF-1 activation with comparable potency (IC50 0.92 μM). The octanoyl (11) and undecanoyl (12) ester derivatives also suppressed HIF-1 activation (IC50 values 3.1 and 0.87 μM, respectively). Mechanistic studies revealed that these geranyloxycoumarin derivatives disrupt mitochondrial respiration, primarily at complex I. Thus, these compounds may inhibit HIF-1 activation by suppressing mitochondria-mediated hypoxic signaling. One surprising observation was that, while less potent, the purported cancer chemopreventive agent auraptene (8) was found to act as a mitochondrial poison that disrupts HIF-1 signaling in tumors. 相似文献
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Phuwapraisirisan P Puksasook T Jong-Aramruang J Kokpol U 《Bioorganic & medicinal chemistry letters》2008,18(18):4956-4958
A series of phenylethyl cinnamides, which included new compounds named anhydromarmeline, aegelinosides A and B, were isolated from Aegle marmelos leaves as alpha-glucosidase inhibitors. The structures of new compounds were characterized by spectroscopic data and chemical degradation. Of compounds isolated, anhydroaegeline revealed the most potent inhibitory effect against alpha-glucosidase with IC(50) value of 35.8 microM. The present result also supports ethnopharmacological use of A. marmelos as a remedy for diabetes mellitus. 相似文献
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Sahare KN Anandhraman V Meshram VG Meshram SU Reddy MV Tumane PM Goswami K 《Indian journal of experimental biology》2008,46(2):128-131
In the present study, methanolic extracts of roots of Vitex negundo L. and extracts of leaves of Vitex negundo L., Ricinus communis L. and Aegle marmelos Corr. were explored for possible antifilarial effect against Brugia malayi microfilariae. It was observed that among the herbal extracts, root extract of Vitex negundo L. and leaves extract of Aegle marmelos Corr. at 100 ng/ml concentration showed complete loss of motility of microfilariae after 48 hr of incubation. Thin layer chromatography of the extracts revealed the presence of alkaloids, saponin and flavonoids in the roots of Vitex negundo L. and coumarin in the leaves of Aegle marmelos Corr. 相似文献
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V. Mallika K.C. Sivakumar G. Aiswarya 《Journal of biomolecular structure & dynamics》2019,37(1):195-209
Quinolone synthase from Aegle marmelos (AmQNS) is a Rutacean-specific plant type III polyketide synthase that synthesizes quinolone, acridone, and benzalacetone with therapeutic potential. Simple architecture and broad substrate affinity of AmQNS make it as one of the target enzymes to produce novel structural scaffolds. Another unique feature of AmQNS despite its high similarity to acridone forming type III polyketide synthase from Citrus microcarpa is the variation in the product formation. Hence, to explore the characteristic features of AmQNS, an in-depth sequence and structure-based bioinformatics analyses were performed. Our studies indicated that AmQNS and its nearest homologs have evolved by a series of gene duplication events and strong purifying selection pressure constrains them in the evolutionary process. Additionally, some amino acid alterations were identified in the functionally important region(s), which can contribute to the functional divergence of the enzyme. Prediction of favorable amino acid substitutions will be advantageous in the metabolic engineering of AmQNS for the production of novel compounds. Furthermore, comparative modeling and docking studies were utilized to investigate the structural behavior and small molecule interaction pattern of AmQNS. The observations and results reported here are crucial for advancing our understanding of AmQNS’s phylogenetic position, selection pressure, evolvability, interaction pattern and thus providing the foundation for further studies on the structural and reaction mechanism. 相似文献
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本文对野生植物三叶木橘的营养成分进行了分析。结果表明,三叶木橘中含有多种营养成分,丰富的矿质元素和 维生素及β-胡萝卜素,其中至少含有17种氨基酸。为开发利用其植物提供了科学依据。 相似文献
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Mohankumar Saraladevi Resmi Priyanka Verma Rajesh S. Gokhale Eppurathu Vasudevan Soniya 《The Journal of biological chemistry》2013,288(10):7271-7281
Quinolone alkaloids, found abundantly in the roots of bael (Aegle marmelos), possess various biological activities and have recently gained attention as potential lead molecules for novel drug designing. Here, we report the characterization of a novel Type III polyketide synthase, quinolone synthase (QNS), from A. marmelos that is involved in the biosynthesis of quinolone alkaloid. Using homology-based structural modeling, we identify two crucial amino acid residues (Ser-132 and Ala-133) at the putative QNS active site. Substitution of Ser-132 to Thr and Ala-133 to Ser apparently constricted the active site cavity resulting in production of naringenin chalcone from p-coumaroyl-CoA. Measurement of steady-state kinetic parameters demonstrates that the catalytic efficiency of QNS was severalfold higher for larger acyl-coenzymeA substrates as compared with smaller precursors. Our mutagenic studies suggest that this protein might have evolved from an evolutionarily related member of chalcone synthase superfamily by mere substitution of two active site residues. The identification and characterization of QNS offers a promising target for gene manipulation studies toward the production of novel alkaloid scaffolds. 相似文献
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This study was designed to elucidate the toxicity of the widely used plant Aegle marmelos in rats. We have taken total alcoholic, total aqueous, whole aqueous and methanolic extracts isolated from the leaves of A. marmelos and studied their toxic effects. Acute, subacute and LD(50) values were determined in experimental rats. The dead animals were obtained from primary screening studies, LD(50) value determination experiments and acute studies subjected to postmortem studies. The external appearance of the dead animals, the appearance of the viscera, heart, lungs, stomach, intestine, liver, kidney, spleen and brain were carefully noted and any apparent and significant features or differences from the norm were recorded. Following the chronic administration of A. marmelos for 14 days, the vital organs such as heart, liver, kidney, testis, spleen and brain were carefully evaluated by histopathological studies and any apparent and significant changes or differences from the norm were studied. From the acute administration of A. marmelos, the LD(50) values were determined using graphical method. The hearts stopped in systolic stand-still in the acute experiments. There were no remarkable changes noticed in the histopathological studies after 50 mg/kg body wt of the extracts of A. marmelos when administered intraperitoneally for 14 days successively. Pathologically, neither gross abnormalities nor histopathological changes were observed. After calculation of LD(50) values using graphical methods, we found a broad therapeutic window and a high therapeutic index value for A. marmelos extracts. Intraperitoneal administration of the extracts of the leaves of A. marmelos at doses of 50, 70, 90 and 100 mg/kg body wt for 14 consecutive days to male and female Wistar rats did not induce any short-term toxicity. Collectively, these data demonstrate that the extracts of the leaves of A. marmelos have a high margin of drug safety. 相似文献