心心相印的组织培养与快速繁殖

1植物名称心心相印(Ceropegia woodii Schlecht.)。2材料类别茎段。3培养条件(1)芽诱导培养基:MS IBA0.1mg·L-1(单位下同) 6-BA1.0 蔗糖30g·L-1;(2)继代增殖培养基:MS IBA0.1 6-BA0.5 蔗糖3     

小叶栒子的组织培养

1植物名称小叶枸子(Cotoneaster microphyllus). 2材料类别茎尖及带腋芽的茎段. 3培养条件芽诱导培养基:(1)MS 6-BA 1.0 mg·L-1(单位下同) IBA 0.1;增殖培养基:(2)MS 6-BA0.8 IBA 0.3,(3)MS 6-BA 0.3 IBA 0.3,(4)MS 6-BA 0.1 IBA 0.5,(5)MS 6-BA 0.3 IBA 0.5,(6)MS 6-BA 0.5 IBA 0.5,(7)MS 6-BA 0.1 IBA0.5 TDZ 0.005;生根培养基:(8)1/2MS IBA 0.1,(9)1/2MS IBA 0.2,(10)1/2MS IBA 0.3,(11)1/2MS IBA 0.4,(12)1/2MS IBA 0.5,(13)1/2MS IBA 0.6.培养基均加入0.5%琼脂,诱导、增殖培养基加3%蔗糖,生根培养基加2%蔗糖,pH 6.0.光照度1500～2000 lx,光照时间10 h·d-1.培养温度15～25℃.空气湿度40%～70%.     

羽叶南洋参的组织培养

1植物名称羽叶南洋参(Polyscias fruticosa var.plumata Bailey),别名南洋森和福禄桐。2材料类别茎段。3培养条件基本培养基为MS。芽诱导培养基:(1)MS 6-BA2mg·L-1(单位下同) NAA0.5。芽增殖     

野扇花的组织培养和快速繁殖

1植物名称野扇花(Sarcococca ruscifolia Stapf),别名清香桂。2材料类别带腋芽的嫩茎段。3培养条件(1)诱导培养基:MS+6-BA2.0mg·L-1(单位下同)+NAA0.05+LH450;(2)增殖培养基:MS+6-BA0.5+NAA0.01+LH300;(3)生根培养基:1/4MS+NAA0.3+IBA0.2。(1)和(2)培养基均附加3%蔗糖、0.7%琼脂,(3)附加1.5%蔗糖、0.6%琼脂,pH5.8～6.0。培养温度(23±2)℃,光强40μmol·m-2·s-1左右,光照时间12h·d-1(李树丽和石文山2005;李雪等2005)。4生长与分化情况4.1外植体的处理先用洗涤剂将外植体清洗3次,然后在流水下冲洗2～3h。在超净工作台上用0.1…     

百里香的组织培养

1植物名称百里香(Thymus serpyllum var.mongolicus Ronn.)。2材料类别顶芽和腋芽。3培养条件(1)芽诱导培养基:MS基本培养基;(2)增殖培养基:MS 6-BA 1.5 mg·L~(-1)(单位下同) IBA 0.1 0.6%琼脂 3%蔗糖;(3)生根培养基:1/2MS IBA 0.5 0.7%琼脂 2%蔗糖。以上所有培养基均为pH 5.8～6.0,培养温度(24±2)℃,光     

霸王鞭的组织培养与快速繁殖

1植物名称霸王鞭(EuphorbiaroyleanaBoiss)。2材料类别带芽眼茎段。3培养条件(1)芽诱导培养基:MS+6-BA1.0mg·L-1(单位下同)+NAA0.1;(2)增殖培养基:MS+6-BA2.0+KT1.0+NAA0.1;(3)壮苗培养基:MS+NAA1.0+6-BA0.5;(4)生根培养基:MS+IBA1.0+6-BA0.1。以上培养基均添加3%蔗糖、0.55%琼脂,pH值5.8,培养温度25℃,光强30～40μmol·m-2·s-1,光照时间10～14h·d-1。4生长与分化情况4.1芽的诱导取当年生霸王鞭茎段,用自来水将其外部泥土冲洗干净,再用洗涤剂清洗,拔去茎表面的刺,用软毛刷仔细清洗刺座部分,然后于超净台上将茎段转入70%乙…     

霸王的组织培养和植株再生

1植物名称霸王[Zygophyllun xanthoxylun(Bunge) Maxim.]。2材料类别当年生健壮枝条。3培养条件芽诱导培养基:(1)MS 6-BA 0.5 mg·L~(-1)(单位下同) NAA 0.01;继代增殖培养基:(2)MS 6-BA 0.3 NAA 0.5;生根培养基:(3) MS IAA 1.0。以上培养基均附加3%蔗糖(生根培     

黄金香柳的组织培养

1植物名称黄金香柳(Melaleuca bracteata F.Muell.)。2材料类别新梢顶芽或新生侧芽。3培养条件(1)腋芽萌发及愈伤组织诱导培养基:MS 6-BA 0.5 mg·L~(-1)(单位下同) NAA 0.3。(2)继代增殖培养基:MS 6-BA 0.1 NAA 0.5。(3)生根培养基:MS NAA 0.3。上述培养基琼脂用     

风箱果腋芽组织培养

1植物名称风箱果[Physocarpusamurensis(Maxim.)Maxim.]。2材料类别休眠期腋芽。3培养条件(1)诱导培养基:WPM+TDZ0.01mg·L-1(单位下同)+IBA1.0;(2)增殖培养基:MS+6-BA1.1+NAA1.0;(3)壮苗培养基:MS+6-BA0.6+NAA2.0;(4)生根培养基:MS+IBA0.1。以上培养基均添加0.65%琼脂和2.5%蔗糖,在高温灭菌前pH值调至5.8。培养温度为25℃,光照时间16h·d-1,光强为30￣40μmol·m-2·s-1。4生长与分化情况4.1无菌材料的获得风箱果的枝条采自黑龙江省尚志市东北林业大学帽儿山实验林场境内。在室内水培3周后,取饱满的腋芽作外植体。腋芽用70%…     

连钱草的组织培养与植株再生

1植物名称连钱草[Glechomalongituba(Nakai)Kupr.]。2材料类别叶片和茎段(带侧芽)。3培养条件诱导芽培养基:(1)MS+NAA0.2mg·L-1(单位下同),(2)MS+NAA0.2+6-BA1.0,(3)MS+NAA0.2+6-BA2.0,(4)MS+NAA0.2+6-BA3.0;增殖培养基:(5)MS+6-BA2.0￣4.0;生根培养基:(6)1/2MS+IBA0.5+IAA0.2。各培养基中均添加30g·L-1白糖和5g·L-1琼脂,pH5.8。培养温度25℃,诱导和增殖培养的光强为1000￣2000μmol·m-2·s-1,光照时间12h·d-1。4生长与分化情况4.1启动培养取连钱草植株,清洗干净,截取茎段(带侧芽),用70%的酒精处理30s,转入0.1%升汞…     

斑叶兰的组织培养

1 植物名称斑叶兰（Goodyera schlechtendalianaRchh．f．）。 2 材料类别 茎段。     

拟蝶唇兰的组织培养

1植物名称拟蝶唇兰【Psychopsis papilio（Lind1．）H．G．Jones】。 2材料类别幼芽。 3培养条件（1）原球茎诱导培养基：MS＋6-BA2．0mg．L^-1（单位下同）＋NAA0．5：（2）丛生芽诱导及增殖培养基：1g．L。花宝1号＋2g．L。花宝2号＋6-BA2．0＋NAA0．05＋100g．L^-2香蕉汁（花宝1号和花宝2号由美国Hyponex化学公司生产,     

Reproductive biology and conservation genetics of Goodyera procera (Orchidaceae)

Goodyera procera is an endangered terrestrial orchid in Hong Kong. Information on its reproductive biology and pattern of genetic variation is needed to develop efficient conservation strategies. Pollination experiments showed that the species is self-compatible, but dependent on pollinators for fruit set. Bagged plants produced no fruits. Artificial pollinations resulted in 92% fruit set through selfing, 94% with geitonogamous pollination, and 95% following xenogamous pollination. Fruit set in the open-pollinated control was 75% at the same sites. Allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to evaluate genetic variation and structure of 15 populations of Goodyera procera. Despite its outbreeding system, allozyme data revealed low variation both at the population (P = 21.78%, A = 1.22, and H = 0.073) and species (P = 33%, A = 1.33, and H = 0.15) levels, in comparison with other animal-pollinated outbreeding plant species. However, RAPD variation was relatively high (P = 55.13% and H = 0.18 at the population level, and P = 97.03% and H = 0.29 at the species level). G_ST estimates indicated high levels of genetic differentiation among populations (G_ST = 0.52 and I = 0.909 ± 0.049 based on allozyme data, and G_ST = 0.39 and I = 0.859 ± 0.038 based on RAPD data), much above the average for outcrossing species, suggesting that gene flow was limited in this species. Based on these data, suitable strategies were developed for the genetic conservation and management of the species.     

仙人掌的微繁殖

成功建立了仙人掌离体快繁的实验体系 ,并且对影响微繁殖的一些因素 ,诸如激素组合、外植体的物理状态、大量元素的含量等进行了研究。结果表明 :BA对仙人掌芽增殖具明显作用 ,MS +BA 5 .0mg/L +IBA 0 .1mg/L为最适增殖培养基 ;接种方式实验表明劈接优于整棵。钙、镁离子浓度对试管苗生长没有影响 ,但影响生根数及根长 ;NAA抑制根的伸长 ,但一定浓度可促进生根。总体而言 ,最适的生根培养基为 1 /2MS。同时发现块接比单芽接具有优势。试管苗在形态上出现一些变异。实验结果对仙人掌科其它植物的快速繁殖具有参考意义     

广东石斛的组织培养

1植物名称广东石斛(Dendrobium wilsonii Rolfe). 2材料类别幼芽.     

A New Steroidal Glycoside from Ophiopogon japonicus （Thunb.） Ker-Gawl.

To study the chemical constituents from traditional Chinese herb Ophiopogon japonicus （Thunb.） Ker-Gawl., a new steroidal glycoside, named ophiopojaponin C （1）, together with two known ones, was isolated by column chromatography. Spectroscopic and chemical evidence revealed the structures to be ophiopogenin 3-O-[α-L-rhamnopyranosyl（1→2）]-β-D-xylopyranosyl（1→4）-β-D-glucopyranoside （1）, diosgenin 3-O-[2-O-acetyl-α-L-rhamnopyranosyl（1→2）]-β-D-xylopyranosyl（1→3）-β-D-glucopyranoside （2）, and ruscogenin 1-O-[2-O-acetyl-α-L-rhamnopyranosyl（1→2）]-β-D-xylopyranosyl（1→3）-β-D-fucopyranoside （3）.     

A New Steroidal Glycoside from Ophiopogon japonicus (Thunb.) Ker-Gawl.

To study the chemical constituents from traditional Chinese herb Ophiopogon japonicus(Thunb.) Ker-Gawl., a new steroidal glycoside, named ophiopojaponin C (1), together with two known ones,was isolated by column chromatography. Spectroscopic and chemical evidence revealed the structures to be ophiopogenin 3-O-[α-L-rhamnopyranosyl(1→2)]-β-D-xylopyranosyl(1→4)-β-D-glucopyranoside (1),diosgenin 3-O-[2-O-acetyl-α-L-rhamnopyranosyl( 1 →2)] -β-D-xylopyranosyl( 1 →3)-β-D-glucopyranoside (2),and ruscogenin 1-O-[2-O-acetyl-α-L-rhamnopyranosyl(1→2)]-β-D-xylopyranosyl(1→3)-β-D-fucopyranoside(3).     

观音兰的组织培养与快速繁殖

1植物名称观音兰[Tritonia crocata (Thunb) Ker-Gawl.]。2材料类别球茎和根。3培养条件(1)球茎诱导芽的分化培养基:MS BA2.0mg·L-1(单位下同) NAA0.5;(2)根诱导愈伤组织的培养基:MS BA0.5 NAA0.1 2,4-D     

岗梅的组织培养和快速繁殖

1植物名称岗梅[Ilexasprella(Hook.etArn.)Champ.exBenth.],别名称星树。2材料类别嫰枝。3培养条件芽启动诱导培养基:(1)MS+6-BA2.0mg·L-1(单位下同)+NAA0.05+3%蔗糖。增殖培养基:(2)MS+6-BA1.0+NAA0.01+2%蔗糖。生根培养基:(3)1/2MS+NAA0.1+2%蔗糖+0.1%活性炭(AC);(4)1/2MS+NAA0.5+2%蔗糖+0.1%AC;(5)1/2MS+NAA1.0+2%蔗糖+0.1%AC。以上培养基均附加0.7%的琼脂,pH5.8。光强27～36μmol·m-2·s-1,光照时间为10h·d-1;培养温度为(28±2)℃。4生长与分化情况4.1无菌材料的获得取10～15cm的嫰枝条,切去叶片,在超净工作台上,…