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1.
Patterns in the distribution of intracellular ATP concentration in relation to coordination of amoeboid cell behavior in Physarum polycephalum 总被引:3,自引:0,他引:3
The Physarum plasmodium reacts tactically to external stimuli. The cell behavior of this giant amoeboid cell was studied by analysing intracellular ATP concentration. The two-dimensional (2D) spatial distribution of ATP depended on cell shape: a polar pattern for a unidirectionally migrating plasmodium, a bowl shape for a circular plasmodium, a hump shape for an oval plasmodium, or a wavy pattern for plasmodia stimulated with blue light or confined in a small chamber, etc. Local external stimulation brought about new patterns of ATP distribution. The ATP concentrations around the stimulated frontal region were reduced by about a half stimulation with KCl (repellent) or casamino acids (attractant). In both cases, migration was inhibited. Migration velocity increased almost linearly with increasing concentration of intracellular ATP above the threshold (about 20 micrograms/mg protein). Under anaerobic conditions or at low temperatures, the intracellular ATP oscillated slowly with a periodicity of about 30 min. Pattern formations in the intracellular ATP concentration and amoeboid coordination are discussed in terms of coupled chemical oscillators in a self-organizing system. 相似文献
2.
Temporal and regional aspects of early neural crest cell migration in relation to extracellular matrix (ECM) organization and distribution in the embryonic axolotl trunk were studied by light microscopy, TEM, and SEM. The dominating structure of the interstitial ECM is a complex network of fibrils, which are indicated by ruthenium red staining to consist of collagen in association with ruthenium red-positive components, probably including glycosaminoglycans. The ECM fibrils, which are largely used as substratum for locomotion by the crest cells, have a temporally and regionally specific organization and distribution. Increase in ECM fibrils on the neural tube, ahead of the crest cell front, is correlated with initiation of crest cell emigration, and it is suggested that the fibrils may stimulate this process by providing a suitable substratum for cell locomotion. An increase in ECM fibrils in extracellular spaces surrounding the crest cell population is correlated with an expansion of these spaces and with progressing crest cell migration into them. It is proposed that the spatial organization of the ECM fibrils influences crest cell shape and orientation during early migration. 相似文献
3.
Summary R-HMM (rhodamine-heavy meromyosin) stained the birefringent fibrous structure which appears and disappears cyclically in parallel with the periodic shuttle streaming in the plasmodium ofPhysarum polycephalum. In addition, 0.6 M KI readily made the birefringent fibrils fade away. These results clearly show that the birefringent fibrils are composed of actin filaments and prove the possibility of actin filaments to alter in the aggregation state during the cyclic production of the motive force responsible for the cytoplasmic streaming. 相似文献
4.
Changes in cell shape and size were measured during locomotion, together with the motive force of the protoplasmic streaming, in various amoeboid cells in different stages of their life cycle, and under various environmental conditions. The variations in these measurements with time were examined by Fourier spectral analysis. Notwithstanding a change in cell type in the life cycle of P. polycephalum, myxamoebae and tiny plasmodia showed a similar time pattern of locomotion, exhibiting oscillations having a mixture of several periods. A regular oscillation with protoplasmic streaming appeared in the plasmodium only above a critical cell size. D. discoideum amoebae oscillated with two periods of a few minutes in preaggregation stage, but with a period of 10 min in aggregation stage, the latter being induced by cAMP. Macrophages and A. proteus also oscillated with periods of a few minutes. Periods of all these oscillations were prolonged severalfold by respiratory inhibition with NaCN, but were unaffected by glycolytic inhibition with 2-deoxyglucose. Cell fragments of A. proteus containing fewer granules oscillated more slowly and with a larger amplitude than those containing more granules. Among the granules, the nucleus was excluded as a possible modifier of the oscillation. The oscillation in Physarum plasmodium was reversibly suppressed by combining respiratory and ATPase inhibitions in mitochondria with NaCN and oligomycin, intracellular ATP concentration being kept at an appropriate level. The present results show that amoeboid motility, as well as cell shape, is oscillatory and that mitochondria are involved in time keeping. 相似文献
5.
Tetsuo Ueda Kenji Matsumoto Tatsuo Akitaya Yonosuke Kobatake 《Experimental cell research》1986,162(2)
Spatio-temporal organization of a migrating plasmodium was studied both by analysing intracellular concentrations of adenine and cyclic nucleotides and by applying image processing for recording oscillatory changes in thickness with use of microcomputers. ATP and ADP concentrations were about twice as high in the front as in the rear, while AMP distributed uniformly. On the other hand, cAMP and cGMP concentrations were several times higher in the rear than in the front, showing oscillations in between. The cAMP concentrations at the front oscillated with a phase advancing about one-third of the period with respect to the phase of the thickness oscillation, while cGMP concentration there varied only little. ATP concentration oscillated concomitantly with H+. A feedback control loop consisting of (ATP-H+)-cAMP-Ca2+ is proposed. The possible mechanism of rhythmic contractions involving mitochondria which may excrete pulses of Ca2+ and induce cell polarization is discussed. 相似文献
6.
THE CHANGING PATTERN OF BIREFRINGENCE IN PLASMODIA OF THE SLIME MOLD, PHYSARUM POLYCEPHALUM 总被引:7,自引:5,他引:2
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Plasmodia of the acellular slime mold, Physarum polycephalum, reveal a complex and changing pattern of birefringence when examined with a sensitive polarizing microscope. Positively birefringent fibrils are found throughout the ectoplasmic region of the plasmodium. In the larger strands they may be oriented parallel to the strand axis, or arranged circularly or spirally along the periphery of endoplasmic channels. Some fibrils exist for only a few minutes, others for a longer period. Some, particularly the circular fibrils, undergo changes in birefringence as they undergo cyclic deformations. In the ramifying strand region and the advancing margin there is a tendency for fibrils of various sizes to become organized into mutually orthogonal arrays. In some plasmodia the channel wall material immediately adjacent to the endoplasm has been found to be birefringent. The sign of endoplasmic birefringence is negative, and its magnitude is apparently constant over the streaming cycle. The pattern of plasmodial birefringence and its changes during the shuttle streaming cycle of Physarum are considered in the light of several models designed to explain either cytoplasmic streaming alone or the entire gamut of plasmodial motions. The results of this and other recent physical studies suggest that both streaming and the various other motions of the plasmodium may very likely be explained in terms of coordinated contractions taking place in the fibrils which are rendered visible in polarized light. 相似文献
7.
Summary The birefringent fibrils in thin-spread plasmodium ofPhysarum polycephalum have been investigated with both polarizing and electron microscopes. The birefringent fibrils were classified into three groups by polarized light microscopy. The first type of fibril is observed in the advancing frontal region as a mutual orthogonal array. The birefringence changes rhythmically in accordance with the shuttle streaming. The second type of birefringent fibril is located in the strand region and runs parallel or somewhat oblique to the strand axis. The third type is observed in the strand region always perpendicular to the streaming axis. Electron microscopy confirmed that all these fibrils are composed of microfilaments, which range in densities in the cross view of the fibril from 1.2 to 1.7 × 103/m2 (1.5 × 103/(xm2 on the average). 相似文献
8.
The spreading behaviour of dissociated hypoblast cells on and besides a band of aligned fibrils associated with the basal lamina of the epiblast was investigated by the use of scanning electron microscopy. A horse-shaped band of aligned fibrils, first demonstrated by Wakely and England (1979), is present during the gastrulation stages of chicken embryos on the ventral side of the epiblast at the cranial and lateral borders of the area pellucida. The basal lamina of the area pellucida situated inside the fibrillar band enables the spreading and probably the locomotion of dissociated cells, which appeared as polarized cells. Numerous cells were also found on the fibrillar band, and these cells lacked distinct lamellae and a polarized shape. Extensions of the cells contacted the extracellular fibrils and, at these sites of contact, the pattern of the fibrils was frequently deformed. From these observations and from previous results emerged the concept that spreading and locomotion of dissociated hypoblast cells, as well as single mesoblast cells and healing hypoblast epithelium, are inhibited by the band of extracellular fibrils, which acts as a physical barrier. The cell biological basis of the mechanism by which extracellular fibrils associated with the basal lamina arrest the migration of hypoblast and mesoblast cells, but guide the migration of primordial germ cells, is discussed. 相似文献
9.
Summary Phototactic responses in a giant amoeboid cell ofPhysarum plasmodium were studied both by analyzing intracellular ATP content and by applying image processing for recording oscillatory changes in thickness with use of a microcomputer. The ATP content fluctuated and deviated from the initial level upon exposure to light with varying wavelength from 400 to 600 nm. The maximum decrease in the integrated value
dt with T=9 minutes occurred at the wavelength 450 nm. The ATP in a migrating plasmodium distributed twice as high in the front as in the rear. This polar pattern became unstable, and a new wavy pattern appeared by stimulating a local frontal part with blue light. In a concentrically extending plasmodium, peripheral and inside regions oscillated in opposite phase to each other. When part of this organism was exposed to light, stimulated and unslimulated regions began to oscillate in opposite phase, and phase waves propagated inward the stimulated region. And the protoplasm there became thinner, the strongest avoidance reaction occurring to 450 nm light as in ATP response. Phototactic behavior inPhysarum is discussed in terms of bifurcation in spatio-temporal organization appearing in a self-organizing system. 相似文献
10.
Summary The development of a spatio-temporal pattern of Ca2+ concentration by a plasmodium ofPhysarum polycephalum during chemotaxis was studied using fura-2. Whenever the cell displayed coordinated migration in one direction as a whole body, a spatiotemporal pattern was established with a characteristic feature along the longitudinal axis. Calcium concentration oscillated with a period of a few minutes within the cell; the mean concentration at the front was higher than that at the rear. When the cell was given an attractant only at the rear end, the mean concentration rose at the site of application with an immediate increase in the frequency of oscillation. First, the change of the frequency is propagated toward the other end and then the mean level of the Ca2+ concentration at the non-stimulated site decreases. As a result, the Ca2+ gradient is reversed along the cell, which then begins to migrate in a coordinated manner in the reverse direction. This study showed that the spatiotemporal pattern of Ca2+ concentration is closely related to information processing for coordinated migration in chemotaxis. The role of the pattern in that process is discussed. 相似文献
11.
Dynamic organization of Physarum plasmodium 总被引:1,自引:0,他引:1
Birefringent fibrils (BRFs) with a positive sign composed of bundles of F-actin were found throughout the Physarum plasmodium with the mode of existence differing regionally. In the zone behind the leading edge of an advancing plasmodium, where cytoplasmic sol and gel were still not well differentiated, more BRFs came to the foreground when the endoplasm flowed backward (emptying phase), and a substantial portion disappeared when the endoplasm flowed forward (filling phase), except for nodes, from which BRFs were reorganized in the early emptying phase of each cycle. BRFs found in the wall of the streaming channel in the posterior network and the branched vein section ran in parallel to or helically around the channel. They were much more stable and maintained strong birefringence irrespective of the direction of the cytoplasmic flow. When the fan-like expanse ceased moving forward, the BRFs no longer appeared and disappeared cyclically but persisted in the area which had previously been the front. We concluded that the site of the active contraction-relaxation rhythm in an advancing plasmodium with antero-posterior polarity is restricted to its frontal zone and that the rest of the plasmodium is in a state of "tonus" which continuously imparts a certain level of hydrostatic pressure to the interior. The meaning of the tonus and the mechanics of tensile force production in the plasmodium are discussed in terms of a working hypothesis arrived at from the phase relationship between isometric and isotonic contraction waves. 相似文献
12.
N. B. Matveeva V. A. Teplov S. I. Beylina 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2012,6(3):255-264
The possibility of tight coupling of phospholipase C with the signal pathway PI3K/ PTEN, a ubiquitous mechanism for the control of chemotaxis and cell shape in free-living amoebae and mammalian tissue cells, has been investigated in Physarum polycephalum plasmodium, a multinuclear amoeboid cell with the autooscillatory mode of motility. It was found that on the maintenance of contractile autooscillations and protoplasmic shuttle streaming, U73122, an inhibitor of the signal transduction to phospholipase C, induces degradation of the plasmodium frontal zone, decreases efficiency of locomotion and suppresses the chemotaxis toward glucose as well as the response of oscillator to this attractant. The identity of the effects of U73122 with those shown for wortmannin and LY294002, widely used PI3K inhibitors (Matveeva et al. 2008. Biophysics. 53, 533–538), suggests a tight coupling of the signal pathways of phospholipase C and PI3K/PTEN. U73122 increases the period of contractile oscillations and abolishes its cyclic changes attributed for the plasmodium migration. The results indicate that motile behavior of the plasmodium is under the receptor-mediated control. 相似文献
13.
Rana pipiens eggs fertilized by Rana esculenta sperm (ESC) hybrid embryos develop until gastrulation in control Rana pipiens embryos (PIP) and then show morphogenetic arrest. After arrest, ESC do not gastrulate but live for 5 days as blastula-like embryos. We studied the distribution of fibronectin (FN)-containing fibrils and integrin (INT) in PIP and ESC. There are many FN-fibrils in PIP organized in anastomosing networks radiating away from the center of individual cells and across intercellular boundaries. ESC have fewer fibrils compared to PIP. These fibrils are first located between cells in disorganized arrays. After arrest in ESC, when PIP are Stage 14 neurulae, many more FN-fibrils appear. INT-staining occurs in both embryos in similar patterns. In xenoplastic transplantations, we found that the extracellular matrix on the inner surface of the ESC blastocoel roof serves as a substratum for PIP cell migration. In an in vitro assay, we found more cell adhesion to FN-substrata in PIP than in ESC. Cell locomotion rates on FN-substrata were 1.70 +/- 0.85 microns/min for PIP but only 0.46 +/- 0.56 microns/min for ESC. We also found that the inner surface of the blastocoel roof from ESC can not promote cell adhesion and locomotion when Stage 11 fragments are used for conditioning but that Stage 14 fragments can deposit a FN-fibril-rich extracellular matrix which supports PIP mesodermal cell migration at a rate of 1.26 +/- 0.38 microns/min. 相似文献
14.
The present paper is an analytical review of the information available on amoeboid movement in human leucocytes. The reported evidence suggests that leucocyte locomotion is due to pressure developed in the cell cortex in the middle and posterior parts of the moving cell, that 4 nm fibrils may provide at least part of the ultrastructural basis of locomotion, that actin-like and myosin-like proteins may be involved in the mechanism of movement and that ATP may serve as an energy source. Leucocyte motility appears to be governed mainly by factors produced in the external medium. Neutrophil chemotaxis is the most antitubulin-susceptible cell mechanism known; from this observation an essential role of microtubule redistribution in chemotaxis is inferred. In contrast, the random movement of neutrophils is not appreciably affected by antimitotic concentrations of antitubulins. Amoeboid movement seems to be an important mechanism in the short-distance locomotion and immunological functions of leucocytes. 相似文献
15.
John Aronson 《The Journal of cell biology》1963,19(1):107-114
Fibrils from the indirect flight muscle of Drosophila melanogaster which have been teased into a solution containing 0.1 M KCl, 2 mM EDTA, 4 mM MgCl2, and 2.5 mM ATP at pH 7.0 can be made to shorten to 10 per cent of their initial length by reducing the level of ATP at a pH of about 8 or by briefly treating the fibrils with trypsin before lowering the level of ATP. Fibrils shortened in either of these ways, when dehydrated and immersed in nitrobenzene, display a strong positively birefringent band at the level of the Z band. In the trypsin-treated fibrils the width of this Z band increases as the fibril shortens. The data obtained are in agreement with the view that the positively birefringent Z band results from the interdigitation of A filaments in adjacent sarcomeres. With shortening to about 35 per cent of the initial length, the cytological pattern suggests that the A filaments of alternate as well as of adjacent A regions interdigitate. 相似文献
16.
Development of the Basal Lamina and Its Role in Migration and Pattern Formation of Primary Mesenchyme Cells in Sea Urchin Embryos 总被引:4,自引:3,他引:1
Shonan Amemiya 《Development, growth & differentiation》1989,31(2):131-145
The development and substructure of the basal lamina and its role in migration and pattern formation of primary mesenchyme cells (PMCs) in normal as well as Li+ - and Zn++ -treated embryos of sea urchins were investigated by electron microscopy. Major findings were as follows. 1) Network fibrils appear along the basal surface of the blastular wall by the hatching blastula stage. The area covered with fibrils is restricted to the vegetal hemisphere at earlier stages, but extends to the animal hemisphere as development proceeds. 2) Nonfibrous fuzzy material embeds the fibrils to form a basal lamina, but in places the fibrils project from the basal lamina into the blastocoel. The major components of the fuzzy material were digested by glycosidase, which failed to digest the fibrous components. 3) The fibrils can be classified into two types, one Ca++ -independent and the other Ca++ -dependent. PMCs apparently utilize the Ca++ -indepndent fibrils as a substratum for locomotion. 4) After migration, PMCs accumulate in a specific region to form the PMC pattern. This is formed in the area of greatest concentration of Ca++ -independent fibrils. 5) PMCs in embryos treated with LiCl, in contrast to normal embryos, accumulate in the animal pole region where the Ca++ -independent fibrils are markedly concentrated. 相似文献
17.
M Osborn K Weber W Naib-Majani H Hinssen W Stockem K E Wohlfarth-Bottermann 《European journal of cell biology》1983,29(2):179-186
The acellular slime mold Physarum forms very thin plasmodia when sandwiched between two agar sheets. After extraction with glycerol-containing buffers, suitable objects for immunofluorescence microscopy are obtained, and an analysis of the cytoskeletal and contractile system of Physarum becomes possible. Plasmodia were stained with antibodies against myosin and fragmin, a protein factor involved in actin filament length regulation. The microanatomy and topography of cellular structures containing these proteins were investigated at the light and electron microscopic levels. The patterns obtained with the two antibodies are closely related to those obtained with actin antibody [25]. In both cases the complex system of cytoplasmic fibrils is stained selectively. The fibrils form a more or less regular network in the advancing front zone with the fibrils being interconnected by focal nodes. In the posterior region of the plasmodium, where endoplasmic pathways and protoplasmic veins are differentiated, larger fibrils are detected, running obliquely or longitudinally to the veins. With both antibodies the fluorescent pattern of the fibrils is continuous without indications of periodic interruptions or striations, which would be expected in the case of sarcomere-like subunits. With anti-myosin unstained patches are frequently seen at or close to the nodes of the fibrillar network in the anterior region. The small lobopodia, which are rich in actin, are apparently not stained by the myosin antibody, a result similar to the situation in "ruffling edges? of cultured vertebrate cells. Electron microscopic investigations of antibody-labeled fibrils in embedded and sectioned plasmodia allow the identification of antibody molecules at specific sites along the fibrils with a different distribution pattern for each of the two antibodies. 相似文献
18.
Possible involvement of extracellular cAMP-specific phosphodiesterase in the control of cell motile behavior has been investigated in Physarum polycephalum plasmodium, a multinuclear amoeboid cell with the autooscillatory mode of motility. It was found that the rate of the hydrolysis of 10 mM cAMP by a partially purified preparation of cAMP-specific phosphodiesterase secreted by the plasmodium in the course of migration decreases 20-30 times under the action of 1 mM dithiothreitol. In the presence of 1-5 mM of this strong reducing agent, the onset of the plasmodium spreading and the transition to the stage of migration were delayed in a concentration-dependent manner. In accordance with the morphological pattern of motile behavior, the duration of the maintenance of high frequency autooscillations, which normally precede the increase in the rate of the spreading and appear also in response to the application of attractants at spatially uniform concentrations, strongly increased by the action of dithiothreitol. The results obtained suggest that the autocrine production of cAMP and extracellular cAMP-specific phosphodiesterase is an important constituent of the mechanism controlling the motile behavior of the Physarum polycephalum plasmodium. 相似文献
19.
Keratocytes derived from the epidermis of aquatic vertebrates are now widely used for investigation of the mechanism of cell locomotion. One of the main topics under discussion is the question of driving force development and concomitantly subcellular force distribution. Do cells move by actin polymerization-driven extension of the lamella, or is the lamella edge extended at regions of weakness by a flow of cytoplasm generated by hydrostatic pressure? Thus, elasticity changes were followed and the stiffness of the leading front of the lamella was manipulated by local application of phalloidin and cytochalasin D (CD). In scanning acoustic microscopy (SAM), elasticity is revealed from the propagation velocity of longitudinal sound waves (1 GHz). The lateral resolution of SAM is in the micrometer range. Using this method, subcellular tension fields with different stiffnesses (elasticity) can be determined. A typical pattern of subcellular stiffness distribution is related to the direction of migration. Cells forced to change their direction of movement by exposure to DC electric fields of varying polarity alter their pattern of subcellular stiffness in relationship to the new direction. The cells spread into the direction of low stiffness and retract at zones of high stiffness. The pattern of subcellular stiffness distribution reveals force distribution in migrating cells; i.e., if a cell moves exactly in a direction perpendicular to its long axis, then the contractile forces are largest along the long axis and decrease toward the short axis. Locomotion in any angle oblique to this axis requires an asymmetric stiffness distribution. Inhibition of actomyosin contractions by La3+ (2 mM), which inhibits Ca2+ influx, reduces cytoplasmic stiffness accompanied by an immediate cessation of locomotion and a change of cell shape. Local release of CD in front of a progressing lamella activates a cell to follow the CD gradient: The lamella thickens locally and is extended toward the tip of the microcapillary. Release of phalloidin stops extension of the lamella, and the cell turns away from the releasing microcapillary. The response to CD is assumed to be the result of local weakening of the cytoplasm due to severing of the actin fibrils. Phalloidin is supposed to stabilize the leading front by inhibition of F-actin depolymerization. These observations are in favor of the assumption that migration is due to an extension of the cell into the direction of minimum stiffness, and they are consistent with the hypothesis that local release of hydrostatic pressure provides the driving force for the flux of cytoplasm. 相似文献
20.
Klein M Seeger P Schuricht B Alper SL Schwab A 《The Journal of general physiology》2000,115(5):599-608
Cell migration is crucial for processes such as immune defense, wound healing, or the formation of tumor metastases. Typically, migrating cells are polarized within the plane of movement with lamellipodium and cell body representing the front and rear of the cell, respectively. Here, we address the question of whether this polarization also extends to the distribution of ion transporters such as Na(+)/H(+) exchanger (NHE) and anion exchanger in the plasma membrane of migrating cells. Both transporters are required for locomotion of renal epithelial (Madin-Darby canine kidney, MDCK-F) cells and human melanoma cells since their blockade reduces the rate of migration in a dose-dependent manner. Inhibition of migration of MDCK-F cells by NHE blockers is accompanied by a decrease of pH(i). However, when cells are acidified with weak organic acids, migration of MDCK-F cells is normal despite an even more pronounced decrease of pH(i). Under these conditions, NHE activity is increased so that cells are swelling due to the accumulation of organic anions and Na(+). When exclusively applied to the lamellipodium, blockers of NHE or anion exchange inhibit migration of MDCK-F cells as effectively as when applied to the entire cell surface. When they are directed to the cell body, migration is not affected. These data are confirmed immunocytochemically in that the anion exchanger AE2 is concentrated at the front of MDCK-F cells. Our findings show that NHE and anion exchanger are distributed in a polarized way in migrating cells. They are consistent with important contributions of both transporters to protrusion of the lamellipodium via solute uptake and consequent volume increase at the front of migrating cells. 相似文献