共查询到20条相似文献,搜索用时 109 毫秒
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Background
MicroRNAs (miRNAs) have been shown to play important roles in regulating gene expression. Since miRNAs are often evolutionarily conserved and their precursors can be folded into stem-loop hairpins, many miRNAs have been predicted. Yet experimental confirmation is difficult since miRNA expression is often specific to particular tissues and developmental stages.Results
Analysis of 29 human and 230 mouse longSAGE libraries revealed the expression of 22 known and 10 predicted mammalian miRNAs. Most were detected in embryonic tissues. Four SAGE tags detected in human embryonic stem cells specifically match a cluster of four human miRNAs (mir-302a, b, c&d) known to be expressed in embryonic stem cells. LongSAGE data also suggest the existence of a mouse homolog of human and rat mir-493.Conclusion
The observation that some orphan longSAGE tags uniquely match miRNA precursors provides information about the expression of some known and predicted miRNAs.5.
Yi-Ju Li Puting Xu Xuejun Qin Donald E Schmechel Christine M Hulette Jonathan L Haines Margaret A Pericak-Vance John R Gilbert 《BMC bioinformatics》2006,7(1):504
Background
Serial Analysis of Gene Expression (SAGE) is a powerful tool to determine gene expression profiles. Two types of SAGE libraries, ShortSAGE and LongSAGE, are classified based on the length of the SAGE tag (10 vs. 17 basepairs). LongSAGE libraries are thought to be more useful than ShortSAGE libraries, but their information content has not been widely compared. To dissect the differences between these two types of libraries, we utilized four libraries (two LongSAGE and two ShortSAGE libraries) generated from the hippocampus of Alzheimer and control samples. In addition, we generated two additional short SAGE libraries, the truncated long SAGE libraries (tSAGE), from LongSAGE libraries by deleting seven 5' basepairs from each LongSAGE tag. 相似文献6.
Minami M Kureha O Mori M Kamitsuji H Suzuki K Irie T 《Applied microbiology and biotechnology》2007,75(3):609-618
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Hirst M Delaney A Rogers SA Schnerch A Persaud DR O'Connor MD Zeng T Moksa M Fichter K Mah D Go A Morin RD Baross A Zhao Y Khattra J Prabhu AL Pandoh P McDonald H Asano J Dhalla N Ma K Lee S Ally A Chahal N Menzies S Siddiqui A Holt R Jones S Gerhard DS Thomson JA Eaves CJ Marra MA 《Genome biology》2007,8(6):R113-12
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