共查询到20条相似文献,搜索用时 15 毫秒
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Expression of HIV-1 Reverse Transcriptase in Murine Cancer Cells Increases Mitochondrial Respiration
Zakirova N. F. Kondrashova A. S. Golikov M. V. Ivanova O. N. Ivanov A. V. Isaguliants M. G. Bayurova E. O. 《Molecular Biology》2022,56(5):723-734
Molecular Biology - Changes in metabolic pathways are often associated with the development of a wide range of pathologies. Increased glycolysis under conditions of sufficient tissue oxygen supply... 相似文献
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Jiong Wang Robert A. Bambara Lisa M. Demeter Carrie Dykes 《Journal of virology》2010,84(18):9377-9389
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Ⅰ型人免疫缺陷病毒(HIV-1)逆转录酶(RT)在抗病毒感染及AIDS治疗药物的设计中是一个重要的靶分子,并且可作为工具酶应用于逆转录PCR等分子生物学研究中。本研究将HIV-1RT基因经PCR扩增并修饰后克隆入大肠杆菌表达载体pBV220,所获重组子所表达的HIV-1RT蛋白占菌体总蛋白的8%左右,且经[~3H]dTTP掺入法证实该重组HIV-1RT具有RT聚合酶活性。用Q-Sepharose层析柱对重组HIV-1RT蛋白进行了初步纯化,所获纯化样品的RT聚合酶比活性(1.7×10~4U/mg)比纯化前的裂解上清提高612倍。 相似文献
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Michael I. Anazodo Elena Duta Horacio Salomon Albert D. Friesen Mark A. Wainberg Jim A. Wright 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1241-1249
Abstract Fully thioated antisense molecules are often cytotoxic and non-specitic in action. GPI2A is thioated at 7 base positions. GPI2A posses sequence-specific activity against HIV-1 gene expression and viral replication without sigdcant cytotoxicity. Partial thioation did not compromise its uptake, cellular distribution and nuclease resistance. 相似文献