Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53

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An intronic GAA repeat expansion in FGF14 causes the autosomal-dominant adult-onset ataxia SCA50/ATX-FGF14

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De novo deletions and duplications at recombination hotspots in mouse germlines

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FANCM regulates repair pathway choice at stalled replication forks

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Global and tissue-specific aging effects on murine proteomes

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Depolarization-dependent Induction of Site-specific Changes in Sialylation on N-linked Glycoproteins in Rat Nerve Terminals

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Highlights

• •Comprehensive sialiomics of isolated rat synaptosomes.
• •Site-specific modulation of sialic acids on surface glycoproteins after brief depolarization.
• •Sialylation as dynamic modification important for synaptic depolarization-dependent processes.

     

Upregulation of CD73 Confers Acquired Radioresistance and is Required for Maintaining Irradiation-selected Pancreatic Cancer Cells in a Mesenchymal State

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Highlights

• •CD73 is one of the most upregulated proteins in the radioresistant cells.
• •CD73 upregulation confers radioresistance and irradiation-induced apoptosis.
• •CD73 confers radioresistance potentially through inactivating protein BAD.
• •Elevated CD73 is required for maintaining the resistant cells in a mesenchymal state.

     

OpenPepXL: An Open-Source Tool for Sensitive Identification of Cross-Linked Peptides in XL-MS

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Highlights

• •OpenPepXL is a new XL-MS identification tool with a high sensitivity.
• •It is available for all common operating systems and remote computing environments.
• •OpenPepXL is open source and supports open OpenPepXL is available as part of OpenMS data formats like mzML and mzIdentML.
• •at https://www.openms.de/openpepxl.

     

Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons

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Highlights

• •Integrated phosphoproteomics and analyses of newly synthesized proteins in neurons.
• •Resource of temporal mGluR-induced signaling pathways upon DHPG stimulation.
• •Validation of PKC, MAPK1, CAMKIIa, and CDK2 in mGluR-activation and signaling.
• •Validation of Intersectin-1 in DHPG-induced AMPAR internalization.

     

Fast Quantitative Analysis of timsTOF PASEF Data with MSFragger and IonQuant

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Highlights

• •MSFragger now supports raw timsTOF PASEF data.
• •IonQuant performs fast and accurate feature detection and quantification.
• •MSFragger and IonQuant provide excellent performance for timsTOF PASEF data.
• •Flexibility allows for complex analyses, such as semi-enzymatic and open search.

     

Discovery of Species-unique Peptide Biomarkers of Bacterial Pathogens by Tandem Mass Spectrometry-based Proteotyping

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Highlights

• •Discovery of peptide biomarker candidates of respiratory tract pathogens S. pneumoniae, H. influenzae, M. catarrhalis and S. aureus as target pathogens.
• •Peptide biomarker candidates were experimentally verified in clinical samples.
• •Targeted MS using promising peptide biomarker candidates shown as proof-of-concept.

     

Perlecan Knockdown Significantly Alters Extracellular Matrix Composition and Organization During Cartilage Development

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Highlights

• •Matrisome content significantly changes with development and perlecan knockdown.
• •Chondrocytes respond to perlecan deficiency by increasing bulk matrisome secretion.
• •Decreased stiffness may be explained by atypical glycosaminoglycan deposition.
• •Elevated COL10A1 expression and chondrocyte hypertrophy indicate early ossification.

     

Discovery of a Redox Thiol Switch: Implications for Cellular Energy Metabolism

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Highlights

• •Develop a TMT-based proteomics tool to profile cysteine persulfides in the cellular proteomes.
• •Discover a Redox Thiol Switch from protein S-glutathioinylation to S-persulfidation (RTS^GS) with implications in the regulation of cellular energy metabolism under oxidative stress.

     

Tandem Mass Tag Approach Utilizing Pervanadate BOOST Channels Delivers Deeper Quantitative Characterization of the Tyrosine Phosphoproteome

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Highlights

• •Detection of low-abundance phosphotyrosine-containing peptides is challenging.
• •Multiplexed TMT allows inclusion of modification(pTyr)-saturated boost channels.
• •Boost channels facilitate selection of pTyr precursor ions for fragmentation.
• •Quantitation depth is increased while maintaining accuracy and precision.

     

MS2Rescore: Data-Driven Rescoring Dramatically Boosts Immunopeptide Identification Rates

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Identification of Novel Kinases of Tau Using Fluorescence Complementation Mass Spectrometry (FCMS)

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The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana

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Parinarioidins A-B,unprecedented flavonoids from the bark of Brosimum parinarioides Ducke

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Predicting Successful Generation and Inhibition of Seizure-like Afterdischarges and Mapping Their Seizure Networks Using fMRI

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Opto-katanin,an optogenetic tool for localized,microtubule disassembly

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