共查询到20条相似文献,搜索用时 15 毫秒
1.
Kohsuke Tsuchiya Shoko Hosojima Hideki Hara Hiroko Kushiyama Mamunur Rashid Mahib Takeshi Kinoshita Takashi Suda 《Cell reports》2021,34(12):108887
- Download : Download high-res image (176KB)
- Download : Download full-size image
2.
B. Geiken J. MasojÍdek M. Rizzuto M. L. Pompili & M. T. Giardi 《Plant, cell & environment》1998,21(12):1265-1273
The effects of cadmium stress (CdCl2 ) on photochemical activity and protein behaviour of photosystem II (PSII) were studied in vivo and in vitro . Treatments of pea ( Pisum sativum ) and broad bean ( Vicia faba ) plants with 0·05–5 m M cadmium (CdCl2 ) modified PSII activity with a resulting increase in electron transfer followed by an inhibition and damage to the oxygen-evolving complex. Pulse-chase experiments with [35 S]methionine in vivo followed by the separation of the radiolabelled thylakoids into grana and stroma exposed regions indicated that the synthesis, degradation and assembly of the D1 protein were greatly affected by cadmium. Initially D1 synthesis increased, later slowing down when the stress became advanced; at the same time the D1 degradation was increased. Binding studies with radiolabelled [14 C]herbicide revealed that the QB pocket activity was also altered. However, the primary consequence of cadmium stress was the disassembly of the stacked regions. The measurements indicated differential tolerance to cadmium stress between the two plant species, which was not caused by either differential metal uptake or binding to the PSII complex. This suggests that the resulting changes in D1 turnover are a consequence of an unknown primary effect of cadmium on the PSII apparatus. However, we show that the higher tolerance to heavy metal stress found in broad bean plants relative to pea is accompanied by stimulation of D1 turnover. These experiments supported by previous data suggest that modulation of D1 turnover under stress is a commonly occurring process. 相似文献
3.
《Cell reports》2023,42(7):112770
- Download : Download high-res image (175KB)
- Download : Download full-size image
4.
During development in the thymus, each T lymphocyte is equipped with one, essentially unique, T cell receptor (TCR)-specificity. Due to its random nature, this process inevitably also leads to the emergence of potentially dangerous T lymphocytes that may recognize ‘self.’ Nevertheless, autoimmune tissue destruction, the cause of diseases such as multiple sclerosis and diabetes, is the exception rather than the rule. This state of immunological self-tolerance is to a large degree based upon a process called ‘negative selection’: prior to joining the circulating lymphocyte pool, immature T cells test their receptor on self-antigens within the thymic microenvironment, and TCR engagement at this immature stage elicits an apoptotic suicide program. We now find evidence that macroautophagy supports the tolerogenic presentation of self-antigens in the thymus. 相似文献
5.
6.
Ashlyn Poole Yong Song Helen Brown Prue H. Hart Guicheng Zhang 《Journal of cellular and molecular medicine》2018,22(7):3270-3277
Food allergies are becoming increasingly prevalent, especially in young children. Epidemiological evidence from the past decade suggests a role of vitamin D in food allergy pathogenesis. Links have been made between variations in sunlight exposure, latitude, birth season and vitamin D status with food allergy risk. Despite the heightened interest in vitamin D in food allergies, it remains unclear by which exact mechanism(s) it acts. An understanding of the roles vitamin D plays within the immune system at the cellular and genetic levels, as well as the interplay between the microbiome and vitamin D, will provide insight into the importance of the vitamin in food allergies. Here, we discuss the effect of vitamin D on immune cell maturation, differentiation and function; microbiome; genetic and epigenetic regulation (eg DNA methylation); and how these processes are implicated in food allergies. 相似文献
7.
Plant tolerance and resistance in food webs: community-level predictions and evolutionary implications 总被引:4,自引:0,他引:4
While evolutionary ecologists emphasize different ways in which plants can evolutionarily respond to herbivory, such as resistance
or tolerance, community ecology has lagged in its understanding of how these different plant traits can influence interactions,
abundance, composition, and diversity within more complex food webs. In this paper, we present a series of models comparing
community level outcomes when plants either resist or tolerate herbivory. We show that resistance and tolerance can lead to
very different outcomes. A particularly important result is that resistant species should often coexist locally with other,
less resistant competitors, whereas tolerant species should not be able to coexist locally with less tolerant competitors,
although priority effects allow them to coexist regionally. We also use these models to suggest some insights into the evolution
of these traits within more complex communities. We emphasize how understanding the differential effects of plant tolerance
and resistance in food webs provides greater appreciation of a variety of empirical patterns that heretofore have appeared
enigmatic.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
8.
热耐受性及温度对食物同化的影响 总被引:2,自引:2,他引:0
研究变色树蜥(Calotes versicolor)的选择体温、热耐受性、温度对食物同化的影响.结果显示:①幼体的选择体温、临界高温和临界低温的平均值分别为32.6、41.7℃和 7.7℃;成体的选择体温、临界高温和临界低温的平均值分别为33.1、42.0℃和8.2℃.②环境温度在26~34℃时,对变色树蜥食物通过时间和摄入能有显著的影响;对表观消化系数(ADC)和同化效率(AE)无显著的影响;在28~34℃时食物通过时间随温度升高而缩短;在26、28℃和30℃时,摄入能小于更高温度的对应值. 相似文献
9.
Repair of UV-B induced damage of Photosystem II via de novo synthesis of the D1 and D2 reaction centre subunits in Synechocystis sp. PCC 6803 总被引:1,自引:0,他引:1
Sass László Spetea Cornelia Máté Zoltán Nagy Ferenc Vass Imre 《Photosynthesis research》1997,54(1):55-62
The repair of ultraviolet-B radiation induced damage to the structure and function of Photosystem II was studied in the cyanobacterium Synechocystis sp. PCC 6803. UV-B irradiation of intact Synechocystis cells results in the loss of steady-state oxygen evolution, an effect accompanied by a parallel loss of both D1 and D2 protein subunits of the Photosystem II reaction centre. Transfer of the UV-irradiated cells to normal growth conditions under visible light results in partial recovery of the inhibited oxygen evolving activity and restoration of the lost D1 and D2 proteins. The extent of recovery decreases with increasing degree of damage: after 50% inhibition, the original activity is completely restored within 2 hours. In contrast, after 90–95% inhibition less than half of the original activity is regained during a 4 hour recovery period. The translation inhibitor lincomycin completely blocks the recovery process if added after the UV-B treatment, and accelerates the kinetics of activity loss if added before the onset of UV-B irradiation. Substantial retardation of recovery and acceleration of activity loss is also observed if the very low intensity short wavelength contribution (<290 nm) is not filtered out from the UV-B light source. It is concluded that in intact cells UV-B induced damage of the Photosystem II complex can be repaired. This process is the first example of simultaneous D1 and D2 protein repair in Photosystem II, and considered to function as an important defence mechanism against detrimental UV-B effects in oxygenic photosynthetic organisms. De novo synthesis of the D1 and D2 reaction centre subunits is a key step of the repair process, which itself can also be inhibited by ultraviolet light, especially by the short wavelength UV-C components, or by high doses of UV-B. 相似文献
10.
Plasticity of photosynthetic heat tolerance in plants adapted to thermally contrasting biomes 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Plant, cell & environment》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Lingling Zhu Keith J. Bloomfield Charles H. Hocart John J.G. Egerton Odhran S. O'Sullivan Aurore Penillard Lasantha K. Weerasinghe Owen K. Atkin 《Plant, cell & environment》2018,41(6):1251-1262
In many biomes, plants are subject to heatwaves, potentially causing irreversible damage to the photosynthetic apparatus. Field surveys have documented global, temperature‐dependent patterns in photosynthetic heat tolerance (P HT); however, it remains unclear if these patterns reflect acclimation in P HT or inherent differences among species adapted to contrasting habitats. To address these unknowns, we quantified seasonal variations in T crit (high temperature where minimal chlorophyll‐a fluorescence rises rapidly, reflecting disruption to photosystem II) in 62 species native to 6 sites from 5 thermally contrasting biomes across Australia. T crit and leaf fatty acid (FA) composition (important for membrane stability) were quantified in three temperature‐controlled glasshouses in 20 of those species. T crit was greatest at hot field sites and acclimated seasonally (summer > winter, increasing on average 0.34 °C per °C increase in growth temperature). The glasshouse study showed that T crit was inherently higher in species from warmer habitats (increasing 0.16 °C per °C increase in origin annual mean maximum temperature) and acclimated to increasing growth temperature (0.24 °C °C?1). Variations in T crit were positively correlated with the relative abundance of saturated FAs, with FAs accounting for 40% of T crit variation. These results highlight the importance of both plastic adjustments and inherent differences determining contemporary continent‐wide patterns in P HT. 相似文献
11.
Dongho Geum Woong Sun Sang Ki Paik Chung Choo Lee Kyungjin Kim 《Molecular reproduction and development》1997,46(4):450-458
D-type cyclins are involved in the regulation of the G1/S transition of the cell cycle in various cell types cultured in vitro. Little is, however, known about the expression pattern and functional role of D-type cyclins in physiological processes in vivo. In this report, we studied whether the expression of murine D-type cyclins correlates with the states of mouse uterine cell proliferation in vivo. Time-course changes in cyclin D1 and D3 mRNA levels in the uterine tissues of immature mice primed with 17β-estradiol (E2) were examined by Northern blot hybridization. c-fos and thymidine kinase (TK) mRNA levels were also examined as markers for the transition from G0 to G1 and the onset of S phase, respectively. Cyclin D1 and D3 mRNAs were induced 2.5-fold between c-fos and TK mRNA peaks. The E2-induced cyclin D1 and D3 gene expressions were blocked by antiestrogens tamoxifen and ICI 182,780. We also investigated the effects of cycloheximide (CHX), a protein synthesis inhibitor, on cyclin D1 and D3 gene expressions. When CHX was treated alone, cyclin D3, but not cyclin D1, mRNA was immediately superinduced. The E2-induced cyclin D3 gene expression was shifted by approximately 6 h when CHX was pretreated 1 hr before E2 administration. Interestingly, the 3H-thymidine incorporation experiment showed that the mouse uterine cell cycle progression also shifted by 6 hr with pretreatment of CHX. The overall results suggest that both cyclin D1 and D3 mRNAs are constitutively expressed in uterine tissues and induced by E2 at G1 phase of the mouse uterine cell cycle. However, the superinducibility and temporal shift of cyclin D3 by CHX suggest that there is a different regulatory mechanism underlying cyclin D1 and D3 gene expressions in the mouse uterine cell cycle progression. Mol. Reprod. Dev. 46:450–458, 1997. © 1997 Wiley-Liss, Inc. 相似文献
12.
Enhanced tolerance to drought stress in transgenic rice plants overexpressing a small heat-shock protein,sHSP17.7 总被引:2,自引:0,他引:2
Exposure of rice (Oryza sativa L.) seedlings to a high temperature (42°C) for 24 h resulted in a significant increase in tolerance to drought stress. To
try to determine the mechanisms of acquisition of tolerance to drought stress by heat shock, the rice small heat-shock protein
gene, sHSP17.7, the product of which was shown to act as molecular chaperones in vitro and in vivo in our previous study, was overexpressed
in the rice cultivar “Hoshinoyume”. Western and Northern blot analyses showed higher expression levels of sHSP17.7 protein
in three transgenic lines than in one transgenic line. Drought tolerance was assessed in these transgenic lines and wild-type
plants by withholding water for 6 days for evaluation of the ability of plants to continue growth after water-stress treatments.
Although no significant difference was found in water potential of seedlings between transgenic lines and wild-type plants
at the end of drought treatments, only transgenic seedlings with higher expression levels of sHSP17.7 protein could regrow
after rewatering. Similar results were observed in survival rates after treatments with 30% polyethylene glycol (PEG) 3640
for 3 days. These results suggest that overproduction of sHSP17.7 could increase drought tolerance in transgenic rice seedlings. 相似文献
13.
Daniel T. Baran John M. Quail Rahul Ray Thomas Honeyman 《Journal of cellular biochemistry》2001,80(2):259-265
We have recently reported that annexin II serves as a membrane receptor for 1α,25‐(OH)2D3 and mediates the rapid effect of the hormone on intracellular calcium. The purpose of these studies was to characterize the binding of the hormone to annexin II, determine the specificity of binding, and assess the effect of calcium on binding. The binding of [14C]‐1α,25‐(OH)2D3 bromoacetate to purified annexin II was inhibited by 1α,25‐(OH)2D3 in a concentration‐dependent manner. Binding of the radiolabeled ligand to annexin II was markedly diminished by 1α,25‐(OH)2D3 at 24 μM, 18 μM, and 12 μM and blunted by 6 μM and 3 μM. At a concentration of 12 μM, 1β,25‐(OH)2D3 also diminished the binding of [14C]‐1α,25‐(OH)2D3 bromoacetate to annexin II, but cholecalciferol, 25‐(OH)D3, and 24,25‐(OH)2D3 did not. Saturation analyses of the binding of [3H]‐1α,25‐(OH)2D3 to purified annexin II showed a KD of 5.5 × 10−9 M, whereas [3H]‐1β,25‐(OH)2D3 exhibited a KD of 6.0 × 10−9 M. Calcium, which binds to the carboxy terminal domain of annexin II, had a concentration‐dependent effect on [14C]‐1α,25‐(OH)2D3 bromoacetate binding to annexin II, with 600 nM calcium being able to inhibit binding of the radiolabeled analog. The inhibitory effect of calcium was prevented by EDTA. Homocysteine, which binds to the amino terminal domain of annexin II, had no effect on the binding of the bromoacetate analog to the protein. The data indicate that 1α,25‐(OH)2D3 binding to annexin II is specific and suggest that the binding site may be located on the carboxy terminal domain of the protein. The ability of 1β,25‐(OH)2D3 to inhibit the binding of [14C]‐1α,25(OH)2D3 bromoacetate to annexin II provides a biochemical explanation for the ability of the 1β‐epimer to inhibit the rapid actions of the hormone in vitro. J. Cell. Biochem. 80:259–265, 2000. © 2000 Wiley‐Liss, Inc. 相似文献
14.
John H Georgakopoulos Anna SokolenkoMichael Arkas Georgia SofouReinhold G Herrmann Joan H Argyroudi-Akoyunoglou 《BBA》2002,1556(1):53-64
Light-harvesting complex II (LHCII) prepared from isolated thylakoids of either broken or intact chloroplasts by three independent methods, exhibits proteolytic activity against LHCII. This activity is readily detectable upon incubation of these preparations at 37 °C (without addition of any chemicals or prior pre-treatment), and can be monitored either by the LHCII immunostain reduction on Western blots or by the Coomassie blue stain reduction in substrate-containing “activity gels”. Upon SDS-sucrose density gradient ultracentrifugation of SDS-solubilized thylakoids, a method which succeeds in the separation of the pigment-protein complexes in their trimeric and monomeric forms, the protease activity copurifies with the LHCII trimer, its monomer exhibiting no activity. This LHCII trimer, apart from being “self-digested”, also degrades the Photosystem II (PSII) core proteins (D1, D2) when added to an isolated PSII core protein preparation containing the D1/D2 heterodimer. Under our experimental conditions, 50% of LHCII or the D1, D2 proteins are degraded by the LHCII-protease complex within 30 min at 37 °C and specific degradation products are observed. The protease is light-inducible during chloroplast biogenesis, stable in low concentrations of SDS, activated by Mg2+, and inhibited by Zn2+, Cd2+, EDTA and p-hydroxy-mercury benzoate (pOHMB), suggesting that it may belong to the cysteine family of proteases. Upon electrophoresis of the LHCII trimer on substrate-containing “activity gels” or normal Laemmli gels, the protease is released from the complex and runs in the upper part of the gel, above the LHCII trimer. A polypeptide of 140 kDa that exhibits proteolytic activity against LHCII, D1 and D2 has been identified as the protease. We believe that this membrane-bound protease is closely associated to the LHCII complex in vivo, as an LHCII-protease complex, its function being the regulation of the PSII unit assembly and/or adaptation. 相似文献
15.
16.
研究了青海沙蜥(Phrynocephalus vlangalii)成体的选择体温、热耐受性及食物同化和运动表现的热依赖性。结果显示:选择体温、临界低温和临界高温无显著的两性差异,其平均值分别为33.3、0.9℃和46.9℃。在27-35℃实验温度范围内,体温显著影响日摄食量,表观消化系数(ADC)和同化效率(AE)无显著影响。停顿次数随着体温的升高而降低,至39℃时停顿次数最少,但与37℃和41℃处理下的停顿次数无显著差异。疾跑速在17-39℃范围内随体温升高而加快,在39℃体温下最快。体温大于39℃后速度减慢。在17-27℃体温范围内,随体温的升高持续运动距离无显著差异。持续运动距离在29-41℃体温下大于较低体温(17-27℃)下的测定值。 相似文献
17.
Photoinhibition of PSII and turnover of the D1 reaction-centre protein in vivo were studied in pumpkin leaves (Cucurbita pepo L.) acclimated to different growth irradiances and in low-light-grown moss, (Ceratodon purpureus) (Hedw.) Brid. The low-light-acclimated pumpkins were most susceptible to photoinhibition. The production rate of photoinhibited PSII centres (kPI), determined in the presence of a chloroplast-encoded protein-synthesis inhibitor, showed no marked difference between the high- and low-light-grown pumpkin leaves. On the other hand, the rate constant for the repair cycle (kREC) of PSII was nearly three times higher in the high-light-grown pumpkin when compared to low-light-grown pumpkin. The slower degradation rate of the damaged D1 protein in the low-light-acclimated leaves, determined by pulsechase experiments with [35S]methionine suggested that the degradation of the Dl protein retards the repair cycle of PSII under photoinhibitory light. Slow degradation of the D1 protein in low-light-grown pumpkin was accompanied by accumulation of a phosphorylated form of the D1 protein, which we postulate as being involved in the regulation of D1-protein degradation and therefore the whole PSII repair cycle. In spite of low growth irradiance the repair cycle of PSII in the moss Ceratodon was rapid under high irradiance. When compared to the high- or low-light-acclimated pumpkin leaves, Ceratodon had the highest rate of D1-protein degradation at 1000 mol photons m–2 s–1. In contrast to the higher plants, the D1 protein of Ceratodon was not phosphorylated either under high irradiance in vivo or under in-vitro conditions, which readily phosphorylate the D1 protein of higher plants. This is consistent with the rapid degradation of the D1 protein in Ceratodon. Screening experiments indicated that D1 protein can be phosphorylated in the thylakoid membranes of angiosperms and conifers but not in lower plants. The postulated regulation mechanism of D1-protein degradation involving phosphorylation and the role of thylakoid organization in the function of PSII repair cycle are discussed.Abbreviations Chl
Chlorophyll
- D1*
phosphorylated form of D1 protein
- Fmax and Fv
maximal and variable fluorescence respectively
- kPJ and kREC
rate constants of photoinhibition and concurrent recovery respectively
- LHCII
lightharvesting chlorophyll a/bprotein of PSII
- PFD
photon flux density
Dr. R. Barbato (Dipartimento di Biologia, Universita di Padova, Padova, Italy), Prof. P. Böger (Lehrstuhl fur Physiologie und Biochemie der Pflanzen, Universität Konstanz, Konstanz, Germany), Prof. A. Melis (Department of Plant Biology, University of California, Berkeley, USA), Prof. I. Ohad (Department of Biological Chemistry, Hebrew University, Jerusalem, Israel) and Mr. A. Soitamo (Department of Biology, University of Turku, Turku, Finland) are gratefully acknowledged for the D1-protein-specific antibodies. The authors thank Ms. Virpi Paakkarinen for excellent technical assistance. This work was supported by the Academy of Finland and the Foundation of the University of Turku. 相似文献
18.
M. Lutter 《Genes, Brain & Behavior》2013,12(6):658-665
While it is known that mice lacking melanocortin 4 receptor (MC4R) expression develop hyperphagia resulting in early‐onset obesity, the specific neural circuits that mediate this process remain unclear. Here, we report that selective restoration of MC4R expression within dopamine‐1 receptor‐expressing neurons [MC4R/dopamine 1 receptor (D1R) mice] partially blunts the severe obesity seen in MC4R‐null mice by decreasing meal size, but not meal frequency, in the dark cycle. We also report that both acute cocaine‐induced anorexia and the development of locomotor sensitization to repeated administration of cocaine are blunted in MC4R‐null mice and normalized in MC4R/D1R mice. Neuronal retrograde tracing identifies the lateral hypothalamic area as the primary target of MC4R‐expressing neurons in the nucleus accumbens. Biochemical studies in the ventral striatum show that phosphorylation of DARPP‐32Thr‐34 and GluR1Ser‐845 is diminished in MC4R‐null mice after chronic cocaine administration but rescued in MC4R/D1R mice. These findings highlight a physiological role of MC4R‐mediated signaling within D1R neurons in the long‐term regulation of energy balance and behavioral responses to cocaine. 相似文献
19.