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The electron transport system of Acetobacter suboxydans with particular reference to cytochrome 总被引:13,自引:0,他引:13
R M Daniel 《Biochimica et biophysica acta》1970,216(2):328-341
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The possibility of immobilization of Acetobacter suboxydans cells by entrapment within polyacrylamide gels or by intercellular cross-linking with glutaral-dehyde was investigated. The oxidation of unsubstituted alditols and aldose diethyl dithioacetals by the resultant modified cells has been achieved. 相似文献
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Phosphoenolpyruvate Carboxylation and Aspartate Synthesis in Acetobacter suboxydans 总被引:3,自引:3,他引:0 下载免费PDF全文
Dialyzed extracts of Acetobacter suboxydans ATCC 621 catalyze (14)CO(2) assimilation in the presence of phosphoenolpyruvate and a divalent cation. The formation of (14)C-oxalacetate was demonstrated and found not to be dependent upon the presence of orthophosphate or diphosphonucleotides. Oxalacetate synthesis was stimulated by orthophosphate and inhibited by aspartate. All attempts to demonstrate a reversible carboxylation mechanism have failed. (14)C-aspartate was synthesized when phosphoenolpyruvate, H(14)Co(3) (-), pyridoxal phosphate, and glutamate were added to dialyzed extracts. Chromatographic and spectrophotometric analyses and chemical degradation further demonstrate the presence of a reversible aspartate aminotransferase. The function of oxalacetate synthesis in a bacterium that reportedly lacks an operative tricarboxylic acid cycle is discussed. 相似文献
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