分子影像:转化医学的重要工具和主要路径

随着基础医学与临床医学脱节现象的日益凸显,作为二者之间连接纽带和桥梁的转化医学越来越受到重视.近年来,转化医学在美、欧等西方国家迅速发展,在我国也正成为"十二五"期间医学领域的重要发展方向之一.分子影像作为现代分子生物学与先进医学影像技术相结合的产物,可以利用影像方法对活体内分子的生物化学过程进行定性和定量研究,是将基...     

第三届“走向前沿一国际高级生化和分子细胞生物学暑期培训班”圆满结束

第三届“走向前沿一国际高级生化和分子细胞生物学暑期培训班（the 3rd Toward Cutting Edge：Intemational Advanced Summer School for Biochemistryand Molecular Cell Biology）”于2012年7月2-7日在上海国家会计学院举行。本期培训班的主题是：代谢与代谢相关疾病（Metabolism and Metabolic Diseases）,内容涉及到肥胖、糖尿病、脂肪和脂肪因子、慢性炎症、心血管和动脉硬化等6个领域。本次活动得到了中国科学院、国家自然科学基金委、国际生物化学与分子生物学联盟（IUBMB）、欧洲生物化学与分子生物学联盟（FEBS）等组织和单位的资助。围绕这6个相关领域,分别邀请6位来自国外知名学者担任主讲人（speaker）,6名来自国内知名学者担任辅讲人（tutor）（表1）。共有57名学员参加,其中8名学员来自欧洲,其余学员来自大陆和香港科研院所和高校。     

“第二届走向前沿-IFCC国际高级生化和分子细胞生物学暑期培训班”圆满结束

2011年第二届走向前沿-IFCC国际高级生化和分子细胞生物学暑期培训班(The 2nd Toward cutting-edge:IFCC Advanced Summer School in Biochemistry and Molecular Cell Biology.于7月4-9日在上海佘山森林宾馆举行,共有60名学员参加,学员分别来自大陆以及台湾和香港科研院所和高校。本期夏令营有6名主讲人     

第三届“走向前沿-国际高级生化和分子细胞生物学暑期培训班”圆满结束

第三届走向前沿-国际高级生化和分子细胞生物学暑期培训班(the 3rd Toward Cutting Edge:International Advanced Summer School for Biochemistry and Molecular Cell Biology)于2012年7月2~7日在上海国家会计学院举行。本期培训班的主题是:代谢与     

第三届“走向前沿-IFCC国际高级生化和分子细胞生物学暑期培训班”招收学员通知

详情请登录中国生物化学与分子生物学会网站http://www.csbmb.org.cn/或者是登录培训班网站:http://www.csbmb.org.cn/ifcc2012/查询,或者直接联系我们:联系人:宋娟孙     

《医学科技发展“十二五”规划》明确未来五年的重点任务

2011年11月15日,科技部网站公布国科发计[2011]552号文件,为了贯彻落实《国家中长期科学和技术发展规划纲要(2006—2020年)》,指导医学科技工作发展,科学技术部、卫生部、国家食品药品监督管理局、国家中医药管理局、教育部、国家人口和计划生育委员会、中国科学院、中国工程院、国家自然科学基金委     

Burkholderia sp.NCIMB 10467菌株中原儿茶酸3, 4-双加氧酶基因的分子克隆和生化特性研究

NCIMB 10467是一株木质素降解菌,根据其16S rDNA序列将其重新分类为Burkholderia菌属.研究显示,在NCIMB 10467菌株中,不同的底物可以诱导该菌株对于原儿茶酸的多种代谢形式.根据克隆到的一段原儿茶酸邻位开环酶,即原儿茶酸3,4-双加氧酶(P34D;EC 1.13.11.3)α-亚基的保守序列,通过染色体步移的方法,得到一段9505bp的DNA片段.序列分析显示,在这段9.5 kb的DNA片段中,两个可能的开放阅读框pcaG和pcaH分别编码P34D的α-亚基和β-亚基.将pcaGH克隆并在大肠杆菌中进行表达后,可以检测到P34D的活性.而pcaH在NCIMB 10467菌株中的敲除则使该菌完全丧失了代谢原儿茶酸的能力.由此证实,克隆到的pcaGH基因确实编码原儿茶酸3,4-双加氧酶,并且对于NCIMB 10467菌株对原儿茶酸的代谢是必需的.     

Living scaffolds (specialized and unspecialized) for regenerative and therapeutic medicine

The physical sciences have increasingly demonstrated a significant influence on the life sciences. Engineering in particular has shown its input through the development of novel medical devices and processes having significance to the biomedical field. This review introduces and discusses several fiber generation protocols, which have recently undergone development and exploration for directly handling living cells from which continuous cell-bearing or living threads to scaffolds and membranes have been fabricated. In doing so these protocols have not only demonstrated their versatility but also opened several unique possibilities for the use of these scaffolds in a plethora of biological and medical applications. In particular, these living fibrous structural units could be explored for regeneration purposes, e.g., from accelerated wound healing to combating a wide range of pathologies when coupled with gene therapy. Thus, "living entities" such as these scaffolds could be most useful in surgery/medicine, including its exploration with stem cells for the preparation of unspecialized living scaffolds and membranes.     

Vitamin C for sepsis intervention: from redox biochemistry to clinical medicine

Molecular and Cellular Biochemistry - Vitamin C, also known as ascorbic acid or ascorbate, is a water-soluble vitamin synthesized in plants as well as in animals except humans and several other...     

Micro CT-based multiscale elasticity of double-porous (pre-cracked) hydroxyapatite granules for regenerative medicine

Hundred micrometers-sized porous hydroxyapatite globules have proved as a successful tissue engineering strategy for bone defects in vivo, as was shown in studies on human mandibles. These granules need to provide enough porous space for bone ingrowth, while maintaining sufficient mechanical competence (stiffness and strength) in this highly load-bearing organ. This double challenge motivates us to scrutinize more deeply the micro- and nanomechanical characteristics of such globules, as to identify possible optimization routes. Therefore, we imaged such a (pre-cracked) granule in a microCT scanner, transformed the attenuation coefficients into voxel-specific nanoporosities, from which we determined, via polycrystal micromechanics, voxel-specific (heterogeneous) elastic properties. The importance of the latter and of the presence of one to several hundred micrometers-sized cracks for realistically estimating the load-carrying behavior of the globule under a typical two-point compressive loading (as in a "splitting" test) is shown through results of large-scale Finite Element analyses, in comparison to analytical results for a sphere loaded at its poles: Use of homogeneous instead of heterogeneous elastic properties would overestimate the structure's stiffness by 5% (when employing a micromechanics-based process as to attain homogeneous properties)-the cracks, in comparison, weaken the structure by one to two orders of magnitudes.     

Biochemistry and molecular biology teaching over the past 50 years

The revolution in molecular biology that began nearly 50 years ago has had an enormous impact in the lab, but it also triggered a quieter revolution in undergraduate teaching. How has it affected the content and teaching methodology of undergraduate courses, and is the training that students now receive in the molecular life sciences appropriate to their future career paths?     

The contribution of the Nobel laureates to biochemistry and molecular biology

The Nobel Prize winners works are considered and analyzed. Main attention is paid to the works devoted to biochemistry or problems related to biochemistry. The most prominent biochemical investigations are considered in detail. In some cases it is given the history and chronology of important biochemical discoveries. The survey is devoted due to the 170th anniversary of birth of Alfred Bernhard Nobel--a famous scientist and businessman, the founder of the Nobel Prizes.     

Canadian medical education: 50 years of innovation and leadership.

Over the past 50 years, many Canadian medical educators have pursued ideas and visions, as individuals in the 1950s and 1960s and later in partnership with various national bodies. Relations between universities and national medical organizations have been productive in dealing with issues of postgraduate education and clinical assessment, in particular. From 1970 to 1990, strong education offices and formally trained educators led to many successes in the areas of research in cognition, continuing medical education and clinical assessment. Canadian medical education has now achieved international recognition for its work in all aspects of the continuum of the physician''s education through vision, initiative and cooperation.     

Tissue microarray (TMA) applications: implications for molecular medicine

Modern expression-screening platforms such as complementary DNA (cDNA) arrays allow for high-throughput lead discovery in cancer and other diseases. For evaluation of promising candidate genes, however, in situ analysis of high numbers of clinical tissues samples--for example, by immunohistochemistry or fluorescence in situ hybridisation--is mandatory. Tissue microarray (TMA) technology greatly facilitates such analysis. Minute tissue cores (diameter 0.6 mm) are removed from up to a thousand different conventional paraffin blocks and re-assembled in a single empty paraffin block at predefined positions. Sections of the resulting TMA can be utilised for the range of research applicable to conventional tissue sections. Important advantages of the TMA technology are speed (parallel analysis of up to a thousand tissues), cost efficiency (the same amount of reagents required for a single large-section analysis is sufficient for a thousand samples), and standardisation (the same experimental conditions are applied to all samples). Because of the high numbers of samples usually included in TMAs, they are optimally suited to detect genotype-phenotype associations with high statistical power. Thus, TMA technology will markedly accelerate the transition from basic research to clinical applications.