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1.
The degradation and fragmentation of the northern Ethiopian highlands has resulted in frequent encounters of large carnivores with humans and their livestock. We interviewed 500 randomly selected households to estimate economic impact of livestock predation by spotted hyaena (Crocuta crocuta), leopard (Panthera pardus) and jackal (Canis aureus aureus) in the highlands of northern Ethiopia. The annual mean economic loss per household was approximately U.S.$ 20.2, about 7% of the average annual income of households in the area. Households surveyed reported losses of a total of 3122 livestock to hyaena, leopard and jackal predation over the past 5 years. This loss equated to a total financial loss of U.S.$ 50,381 . Livestock predation incidents of spotted hyaena, leopard and jackal demonstrated that spotted hyaena had a preference for dog, donkey, goat and sheep; leopard for goat, dog and sheep; and jackal for goat and sheep. Livestock predation of spotted hyaena and leopard were mainly during the night. We conclude that assessing depredation problems is important to develop actions for management of either livestock practices or wildlife conservation.  相似文献   

2.
Distribution of Agrobiodiversity in Home Gardens along the Corrientes River, Peruvian Amazon. This paper examines crop species diversity in home gardens of traditional communities along a remote tributary in northeastern Peru. A large-scale survey was conducted of 300 gardens in 15 villages along a 150 km reach of the Corrientes River. Study villages vary notably in community size, ethnicity of residents (Achuar, Urarina, mestizo), and proximity to the regional town (Trompeteros) as well as to oil wells. Gardens were inventoried and interviews conducted with garden tenders to provide data on socioeconomic characteristics of the household and contextual conditions. Multiple regression analyses identified the determinants of species diversity. Results indicate high crop diversity—the highest yet reported for sites in the Amazon basin—particularly among the Achuar people. Garden species diversity is greatest in larger villages and, surprisingly, in those villages located nearer to the oil company. Within villages, households with larger land holdings (and gardens), more in-house labor, and garden tenders who are older and female tend to have more diverse gardens. A small number of households (15/300) were found to hold exceptional diversity, often older and land-wealthier households located on the outskirts of villages. These farmers held, on average, three times the number of species as a typical village garden and 60% of all species encountered in the study.  相似文献   

3.
Global declines of carnivores are related to difficult integration with human land use, in particular conflicts caused by livestock depredation. Spotted hyenas (Crocuta crocuta) live in remarkably close proximity to humans in the degraded and prey-depleted Enderta district, northern Ethiopia. Their diet and interaction with people were investigated in sub-districts close to the regional capital, Mekelle. We interviewed 1,686 randomly selected households from three sub-districts, Debri, Aynalem, and Felege Selam, about livestock management and incidence of depredation from 2005 to 2009. Livestock loss amounted to 492 heads over 5 years; an annual mean of 0.6% worth US$ 7,042. We also performed a survey giving a minimum population estimate of 60 hyenas in the three sub-districts; all but four were found in church forests where they are traditionally tolerated and protected. A total of 1,200 hyena scats were analyzed to determine prey species; the diet contained only domestic species, with sheep being by far the most common prey species. About 5.5% of fecal analysis contained human hairs. We conclude that hyenas depended entirely on domestic prey species, partly through depredation but more importantly through scavenging on (peri-) urban waste. Under the particular local circumstances, continued coexistence appears possible, provided that damage remains tolerable.  相似文献   

4.
The ovine POU1F1 gene is localized on chromosome 1 and it contains five introns and six exons. In different mammalian species some mutations in different exons are associated with different production traits. The aim of our research was to study the POU1F1 gene nucleotide sequence to detect possible polymorphisms and their relationships with milk productive traits in Sarda breed sheep. The study had been conducted on 140 ewes, 4 or 5 years old coming from a farm located in Sardinia. All the animals were multiparous, lactating and in their third to fifth lactation. Individual milk yield had been recorded monthly and for each sample fat, protein, casein, lactose, and somatic cell count values were analysed. A jugular blood sample was collected from each ewe to perform genomic DNA extraction. PCR, SSCP and sequencing analysis were carried out to examine the six exons to highlight possible SNPs. One-way ANOVA was used to analyse association of variants with milk yield and/or its composition. Two novel SNP were found: 121 C>T in the 5′UTR of the fourth intron fragment and 249 G>A in the 3′UTR of the sixth exon fragment. The statistical analysis did not shown association between milk productive traits and the found polymorphisms. However, further investigations about the promoter region or the prophet genes, like the PROP-1, could clarify its exact role in regulating the productive traits in sheep.  相似文献   

5.
Semen from Barbary sheep (Ammotragus lervia), Bighorn sheep (Ovis canadensis), Mouflon sheep (Ovis musimon), Fallow deer (Dama dama), collected by electroejaculation, and semen from Wildebeest antelope (Connochaetes taurinus), collected post mortem, were frozen using a standardized technique and a commercial freezing medium (Triladyl). Sperm quality was assessed by measuring their capacitation status (chlortetracycline assay) in addition to classic assessment: motility, viability (plasma membrane integrity), and acrosome integrity. Sperm cryosurvival measured in terms of recovery (from the initial, pre-freeze values) of motility, viability, and acrosome integrity was relatively high in most species. However, proportion of premature-capacitated spermatozoa (B + AR patterns) increased many times in relation to that of fresh semen: from 8% to 78% in Barbary sheep; from 27% to 61% in Bighorn sheep; and from 12% to 68% in Mouflon sheep. The use of a standardized technique for freezing of spermatozoa from wild ruminant species produced, in most of the cases, acceptable results. This approach may be extensively used to carry out sperm cryopreservation in field conditions. Chlortetracycline assay allowed identifying the same fluorescent patterns observed in spermatozoa from domestic animals and produced additional information on sperm cryosurvival. That is, it revealed those cells that survive freeze–thawing and are potentially fertile: non-capacitated (F pattern) and capacitated acrosome-intact spermatozoa (B pattern).  相似文献   

6.
We cloned the cDNA and genomic DNA encoding for Izumo1 of cashmere goat (Capra hircus) and sheep (Ovis aries). Analysis of 4.6 kb Izumo1 genomic sequences in sheep and goat revealed a canonical open reading frame (ORF) of 963 bp spliced by eight exons. Sheep and goat Izumo1 genes share >99% identity at both DNA and protein levels and are also highly homologous to the orthologues in cattle, mouse, rat and human. Extensive cloning and analysis of Izumo1 cDNA revealed three (del 69, del 182 and del 217) and two (del 69 and ins 30) alternative splicing isoforms in goat and sheep, respectively. All of the isoforms are derived from splicing at typical GT-AG sites leading to partial or complete truncation of the immunoglobulin (Ig)-like domain. Bioinformatics analysis showed that caprine and ovine Izumo1 proteins share similar structure with their murine orthologue. There are a signal peptide at the N-terminus (1–22 aa), a transmembrane domain at the C-terminus (302–319 aa), and an extracellular Ig-like region in the middle (161–252 aa) with a putative N-linked glycosylation site (N205-N-S). Alignment of Izumo1 protein sequences among 15 mammalian species displayed several highly conserved regions, including LDC and YRC motifs with cysteine residues for potential disulfide bridge formation, CPNKCG motif upstream of the Ig-like domain, GLTDYSFYRVW motif upstream of the putative N-linked glycosylation site, and a number of scattered cysteine residues. These distinctive features are very informative to pinpoint the important gene motifs and functions. The C-terminal regions, however, are more variable across species. Izumo1 cDNA sequences of goat, sheep, and cow were found to be largely homologous, and the molecular phylogenetic analysis is consistent with their morphological taxonomy. This implies the Izumo1 gene evolves from the same ancestor, and the mechanism of sperm–egg fusion in mammals may be under the same principle in which Izumo1 plays an important role.  相似文献   

7.
Yu Y  Song X  Du L  Wang C 《Molecular biology reports》2009,36(7):1799-1809
The calcium and integrin binding protein 1(CIB1), is an EF-hand-containing protein that binds many effector proteins including the platelet αIIbβ3 integrin and potentially regulates their functions. Here we report the cloning and characterization of the sheep CIB1 gene. The CIB1 cDNA is 885-bp in size, containing a 45-bp of 5′ untranslated region (UTR), a 264-bp long 3′-UTR and a 576-bp open reading frame that encodes 191 amino acids. The sheep CIB1 cDNA shows 98.3, 92.0, 91.8, 91.3, 90.5 and 90.1% of similarity, at the nucleotide level, to its equivalents in cattle, pigs, rhesus monkey, humans, rats and mice, respectively at the deduced protein level, the corresponding values are more than 94%. The sheep CIB1 gene consisted of seven exons. Quantitative PCR (Q-PCR) showed that CIB1 was widely expressed in different tissues with the highest level in the testis, suggesting that it may play a role in ram fertility. We cloned the sheep CIB2, CIB3 and CIB4 genes and detected their expression patterns in different tissues.  相似文献   

8.
Brucellosis is a zoonosis caused by bacteria of the genus Brucella, which includes nine species: B. melitensis (goats and sheep as the main reservoir hosts), B. abortus (cattle), B. suis (pigs), B. neotomae (desert woodrats), B. ovis (sheep), B. canis (dogs), B. ceti (whales), B. pinnipedialis (pinnipeds), and B. microti (Microtus voles). The epidemic and epizootic situation with brucellosis is accounted for by farm animals, which are the carriers of three main pathogens (B. melitensis, B. abortus, and B. suis). Their ubiquitous distribution is the factor determining global prevalence of the above Brucella species on all continents and in the overwhelming majority of countries. Consistent with the expansion of the pathogen ecological range are the 1990s findings of new Brucella species in marine mammals (whales and pinnipeds) and in some rodents. These bacteria proved to be also pathogenic for terrestrial mammals and humans. All Brucella-infected animals considered in the paper are tentatively divided into two groups. The first includes most of the wild and domestic animal species, birds, and ticks that acquire the infection farm animals, the main hosts of Brucella. The second group includes animals (wild reindeer, hares, bison, and probably saiga antelopes, dogs, and marine mammals) which may carry Brucella regardless of infection prevalence in the main hosts.  相似文献   

9.
T. C. Hawes 《Polar Biology》2008,31(10):1287-1289
Direct observations of feeding behaviour in Branchinecta gaini are described. B. gaini possess sophisticated food manipulation behaviours, using their abdominal appendages to ‘scrape’ at and carry away food items. Scraping behaviour was employed by animals for both detrital feeding and the cannibalisation of dead conspecifics. In the absence of like observations from other non-raptatory species of Branchinecta, it appears that adults of B. gaini occupy an intermediary position between species that feed exclusively on vegetative and detrital material and those (e.g. B. gigas, B. ferox, B. raptor) that are known to be carnivorous. In an Antarctic context, particularly, specialisation towards carnivory would have little adaptive value, while expansion of diet breadth via increased manipulative and digestive flexibility would, on the other hand, be advantageous. These behaviours, moreover, mean that B. gaini plays an active role in the breakdown and recycling of its own nutrient base.  相似文献   

10.
Eight individual rock iguanas (Iguana pinguis) from Anegada Island were relocated to Guana Island by Lazell, 1984–1987, in order to establish a second population reservoir for this endangered species. The species may have originally occupied the entire Puerto Rico Bank. The relocation has been successful and, in the area currently providing the best habitat, we estimate a density of 9 or 12 animals of various age classes per 19 ha. The optimal area contains a sheep exclosure with relatively dense understory vegetation and numerous exotic as well as native species of plants. Iguana activity is concentrated on east facing slopes and ridge-tops that get morning sun. Outside the exclosure most edible ground cover and shrubs have been eaten by sheep, leaving toxic or noxious species, such as Croton or Lantana, in the understory where I. pinguis adults generally forage. Removal of sheep may be critical to continued population growth of these reptiles. Views on relocation or repatriation of other endangered Antillean Iguana species are advanced, with some ideas on minimum viable population sizes and a possible explanation for the extirpation of I. pinguis from much of its former range.  相似文献   

11.
This study presents the binding of ovine factor H (fH) by various serotypes of Borrelia and simultaneously correlates their complement resistance to sheep serum. Affinity ligand binding assay was employed to study the binding of borrelial proteins to ovine recombinant fH and its truncated forms (short consensus repeat, SCR 7 and SCRs 19–20). From a repertoire of 17 borrelial strains, only two strains showed affinity to sheep fH. A ~28-kDa protein of Borrelia burgdorferi sensu stricto (B. burgdorferi s.s., strain SKT-2) bound full-length fH as well as SCRs 19–20. This fH-binding protein was further identified as complement regulator-acquiring surface protein of B. burgdorferi (BbCRASP-1) by MALDI-TOF analysis. Surprisingly, a ~26-kDa protein of Borrelia bissettii (DN127) showed affinity to full-length fH but not to SCR 7 and SCRs19–20. In complement sensitivity assay, both strains—SKT-2 and DN127—were resistant to normal sheep serum. Significant complement resistance of two Borrelia garinii strains (G117 and T25) was also observed; however, none of those strains was able to bind sheep fH. Our study underscores the need of further exploration of fH-mediated evasion of complement system by Borrelia in domestic animals.  相似文献   

12.
The review aimed at searching for DNA structure markers of epigenetic modifications leading to intrauterine growth restriction (IUGR) in three livestock species, mouse and human. IUGR affects mammals by harming their wellbeing and the profitability of breeding enterprises. Of the livestock species, we chose cow, pig and sheep owing to there being many reports on the epigenetics of IUGR. IUGR investigations in human and mouse are particularly numerous, as we are interested in our own wellbeing and the mouse is a model species. We decided to focus on five genes (Igf2r, Igf2, H19, Peg3 and Mest) of known IUGR association, reported in all of those species. Despite the abundance of papers on IUGR, naturally occurring mutations responsible for epigenetic modifications have been described only in human and cow. The effect of induced DNA structural modifications upon epigenetics has been described in mouse and pig. One paper regarding mouse was chosen from among those describing DNA modifications performed to obtain parthenogenetic progeny. Papers regarding pig parthenogenetic progeny described the epigenetics of genes involved in foetal development, with no interference with the genome structure. No reports on DNA modifications altering IUGR epigenetics in sheep were found. Only environmental effects were studied and we could not conclude from the experiment designs whether the gene setup could affect the expression of involved genes, as different populations were not included or not specified within particular experiments. Apparently, DNA markers of IUGR epigenetics exist. It has been reported that the small number of them, occurring naturally, may result from neglecting existing evidence of such selection or health status forecasting markers.  相似文献   

13.
Four Footed Pharmacists: Indications of Self-Medicating Livestock in Karamoja, Uganda.Following observations of goats’ possible self-medication browsing the anti-parasitic plant, Albizia anthelmintica, an ethnobotanical survey was undertaken to examine whether livestock engage in other self-medicating behaviors, and if people also use the same medications. Information was gathered over a five-month period from 147 Karamojong pastoralists and healers using a checklist of questions. There were 124 observations for 50 proposed self-medicating behaviors, primarily eating plants, to treat a total of 35 disease conditions. Of the plant species, 72% were also prepared by informants to treat human or veterinary diseases. Species importance was estimated by four factors: >3 user citations, informant consensus factor >0.4, fidelity level >40% and presence in the local pharmacopoeia. Eight species fulfilled all of these factors, and 12 had at least three. These results provide support for the hypothesis that animals graze specific plants when ill and suggest that people have developed some of their knowledge through animal observation.  相似文献   

14.
Yu WL  Dan H  Lin M 《Current microbiology》2008,56(5):505-509
The internalins InlA and InlC2 are encoded proteins from two strongly immunoreactive clones recently identified by differential immunoscreening of a Listeria monocytogenes serotype 4b genomic expression library during the search of the gene products of L. monocytogenes specifically induced in vivo during infection (Yu WL, Dan H, Lin M. J Med Microbiol 56:888–895, 2007). In this study, we examined the humoral immune response against InlA and InlC2 in various L. monocytogenes-infected hosts using Western blots. InlA and InlC2 were recognized by antibodies in experimentally infected rabbits but not by antisera from rabbits immunized with the heat-killed bacterium. Similar strong immunological reactions to InlA and InlC2 were seen with antisera from infected guinea pigs, cattle, and sheep but not with those from the animals (guinea pigs or sheep) receiving heat-killed bacteria. This study provides the first experimental evidence that InlA and InlC2 are the in vivo induced or upregulated antigens for humoral immune responses that are common to listerial infection of various host species. These two immunogenic proteins may thus be explored as reagents for the laboratory diagnosis of listeriosis or candidates for vaccine development.  相似文献   

15.
Livestock grazing is a common management practise in semi-natural grasslands in Central Europe. Different types of livestock (horses, cattle, sheep) and grazing intensity are known to affect the richness and composition of plant species. However, knowledge of grazing-dependent effects on invertebrates is limited. We examined the influence of horse, cattle and sheep grazing on the richness, abundance and composition of land snail species in 21 calcareous nutrient-poor grassland areas in the northwestern Jura Mountains, Switzerland. Grazing by different livestock species did not affect the species richness, abundance and species composition of land snails. Furthermore, the number of open-land species and the ratio of large- to small-sized snail species or individuals did not differ among the three pasture types. However, independent of livestock species, grazing intensity negatively influenced the snail fauna. Snail species richness, abundance and number of Red list species decreased with increasing grazing intensity. Grazing intensity also affected the occurrence of individual snail species (Truncatellina cylindrica, Cecilioides acicula, Candidula unifasciata and Trichia plebeia). To preserve the snail fauna in nutrient-poor grasslands, pastures can be stocked with horses, cattle or sheep. However, both maximum stocking rate (number of livestock units per hectare) and grazing duration (number of grazing days per year) must be carefully defined for the proper management of the pastures.  相似文献   

16.
Ovis aries is one of the most important agricultural livestock for meat production, and also is an ideal model organism for biological and comparative genomics studies. Many miRNAs have been reported for their important roles in developmental processes in various animals, but there is limited information about O. aries miRNAs. In this study, combining a computational method based on expressed sequence tag (EST) analysis with experimental identification based on small RNA cDNA library, we identified 31 miRNAs belong to 24 families in sheep, 2 of which were novel miRNAs which had never been previously identified in any species. Especially, we cloned 12 miRNAs from the sheep skeletal muscle, which were good candidate miRNAs to be studied about the miRNA-dependant regulated process of muscle development, and we identified four pairs of miRNA/miRNA* and one pair of miRNA-3p/miRNA-5p from sheep EST sequences. Expression analysis indicated that some miRNAs were expressed in a specific tissue, and the pair of miRNA-3p/miRNA-5p and one pair of miRNA/miRNA* had a similar relative expression pattern in some tissues, respectively. Further, we predicted 120 potential target genes of 31 oar-miRNAs on the 3′UTR of O. aries genes. Gene ontology analysis showed that most of these genes took part in the cellular process and metabolic process. Our results enriched the O. aries miRNA database and provided useful information for investigating biological functions of miRNAs and miRNA* in sheep.  相似文献   

17.
More than 50% of Nepal’s population lives in the hill regions, usually with small land holdings, and over 60% are considered below the poverty line. Livestock, and particularly goats, provide these small-scale farmers with about 55% of their on-farm income. We studied goat production in Katteldanda, a mid-hill village of 78 households, mainly Brahmins, in Ghorka District. Subsistence farmers raise six or seven goats and one or two buffalo, and cultivate mainly maize, millet and rice. Tree fodder constituted about 70% of dry matter intake of goats for large parts of the year and was collected by lopping branches from trees on upland, rain-watered, private terraces (bari) near the household compound. Local farmers ranked the fodder trees they considered best and we measured the actual fodder they offered to goats. In addition, we evaluated and ranked 23 fodder species on the basis of laboratory in vitro nutritional and metabolizable energy yields. Using a Mantel test, a significant correlation was found between what the farmers thought was best fodder and fodder offered to goats (Mantel r = 0.398; P = 0.037) but non-significant correlations were found between either what the farmers thought was best fodder or fodder offered to goats and laboratory rankings (Mantel r = −0.027; P = 0.49 and Mantel r = 0.187; P = 0.18, respectively). We concluded that biomass produced and availability throughout the year, in addition to nutritional and energy yield, are important criteria for selecting fodder trees for goat production.  相似文献   

18.
This study investigated the epidemiological and ecological factors to assess the infection pressure of alveolar echinococcosis to human which are living in villages and small towns. Foxes and fox faeces were examined for Echinococcus multilocularis and foxes were observed by radio telemetry in Upper Bavaria, Germany. Forty-three percent of the village foxes (n = 65) had been infected with E. multilocularis. This prevalence rate did not differ significantly from the prevalence among rural foxes, which was 39% (n = 33; χ 2 = 0.12, df = 1, p = 0.727) determined by the intestinal scraping technique. PCR analyses of fox faeces showed a higher infection rate of 35% (n = 26) among rural foxes than among foxes in villages and small towns (26%, n = 69; χ 2 = 0.68, df = 1, p = 0.411). One quarter of the fox faecal samples come from private gardens of residents. The radio-tracking study on 17 foxes showed that foxes preferred the built-up area and grassland outside the villages. Village foxes concentrated their activity within a range of 500 m around the settlement. Sixty-four percent of all bearings for radio-tracked foxes showed positions in areas outside the town, and 36% of bearings were within the settlement. Village foxes, which are infected with E. multilocularis, are able to carry the parasite continuously into settlements and fox faeces present an immediate source of infection to humans, especially within their gardens. Therefore, foxes are responsible for environmental E. multilocularis egg contamination in the vicinity of humans, leading to an infection risk to inhabitants of villages and small towns.  相似文献   

19.
Bidding Customs and Habitat Improvement for Matsutake (Tricholoma matsutake) in Japan. A brief history of Japan’s matsutake production, use, and management is presented, with emphasis on habitat improvement efforts and the land use tradition of iriai. Bidding systems for allotment of matsutake gathering rights are discussed. Three villages with different bidding systems are compared to see what effects the bidding systems have on village finances, matsutake production, and matsutake habitat enhancement. Contrary to expectations, habitat for matsutake was not improved when land owners were guaranteed the gathering and selling rights to matsutake growing on their own lands. Instead, habitat improvement was most successful and matsutake production was highest on community-owned lands in Oka Village, where the iriai tradition is strongest.  相似文献   

20.
Livestock production is the primary source of livelihood and income in most of the high steppe and alpine regions of the Indian Trans-Himalaya. In some areas, especially those established or proposed for biodiversity conservation, recent increases in populations of domestic livestock, primarily sheep and goats, have raised concern about domestic animals competitively excluding wild herbivores from the rangelands. We evaluated the influence of domestic sheep and goat grazing on the habitat use and time budget of the endangered Tibetan argali Ovis ammon hodgsoni in the proposed Gya-Miru Wildlife Sanctuary, Ladakh, India. We asked if the domestic sheep and goat grazing and collateral human activities relegate the argali to sub-optimal habitats, and alter their foraging time budgets. Data were collected on habitat use and time budget of a population of c. 50 argalis before and after c. 2,000 sheep and goats moved onto their winter pasture in the Tsabra catchment of the aforementioned reserve. Following the introduction of domestic sheep and goats, argalis continued to use the same catchment but shifted to steeper habitats, closer to cliffs, with lower vegetation cover, thus abandoning previously used plant communities with denser cover. Argalis’ active time spent foraging also decreased by 10% in response to the presence of livestock. These results suggest a clear disturbance effect of livestock on argalis, and indicate a potential for competition, conceivably a significant disadvantage for argalis in winter when forage availability is minimal.  相似文献   

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