粤北山区亚硬孔菌属的一个新种(英文)

本文报道了采集于广东省阳山县太平洞乡的一个亚硬孔菌属Rigidoporopsis Johan. &Ryv.新种,命名为大孢亚硬孔菌Rigidoporopsis macrospora G.Y.Zheng et Bi。三个国内新纪录:榆硬孔菌R.ulmarius(Fr.)Ryv.;薄皮蜡卧孔菌Ceriporia leptoderma(Berk.&Br.)Ryv.和新西兰背孔菌Chaetoporus novae-zelandiae(G.H.Cuna.)G.H.Cunn. 硬孔菌属和亚硬孔菌属的主要差别是:前者孢子光滑,非淀粉质反应;而后者则为轻微粗糙或具小疣,淀粉质或拟糊精质反应。另外,前者的担子较后者小得多。     

粤北山区亚硬孔菌属的一个新种

本文报道了采集于广东省阳山县太平洞乡的一个亚硬孔菌属Rigidoporopsis Johan. &Ryv.新种,命名为大孢亚硬孔菌Rigidoporopsis macrospora G.Y.Zheng et Bi。三个国内新纪录:榆硬孔菌R.ulmarius(Fr.)Ryv.;薄皮蜡卧孔菌Ceriporia leptoderma(Berk.&Br.)Ryv.和新西兰背孔菌Chaetoporus novae-zelandiae(G.H.Cuna.)G.H.Cunn. 硬孔菌属和亚硬孔菌属的主要差别是:前者孢子光滑,非淀粉质反应;而后者则为轻微粗糙或具小疣,淀粉质或拟糊精质反应。另外,前者的担子较后者小得多。     

华东地区木腐菌研究3.中国薄孔菌属小记及一新记录种

本文总结了我国薄孔菌属Antrodia的种类,目前我国该属共报道有19种,其中脆薄孔菌A. oleracea为中国新记录种。该种的特征是子实体脆质易碎,菌丝系统几乎单系,生殖菌丝占绝大多数,骨架菌丝很少,孢子圆柱形至近纺锤形。根据中国的材料对这个种进行了详细描述和显微结构绘图。该种与软薄孔菌很相近,但脆薄孔菌主要生长在亚热带地区的阔叶树木上,而软薄孔菌生长在针叶树木上。另外,本文还给出了我国薄孔菌属种类的检索表及每个种的担孢子数据。     

新组合欧氏拟木层孔菌——“西方拟木层孔菌”的合法名称

拟木层孔菌属Phellinopsis是最近从木层孔菌属Phellinus中独立出来的一个属。拟木层孔菌属的全部4个种中,只有西方拟木层孔菌P. occidentalis在中国没有分布。简要回顾了西方拟木层孔菌这一名称的历史,其基元异名西方层孔菌Fomes occidentalis属于无效发表,因此将西方拟木层孔菌处理为无效名称,并将该名称所代表种的合法名称欧氏木层孔菌Phellinus overholtsii组合为欧氏拟木层孔菌Phellinopsis overholtsii。     

中国地孔菌属(盘菌目)分类研究

本文报道盘菌目地孔菌属(Geopora Harkn.)我国种类的分类研究成果,共描述4个种即砂生地孔菌(Geopora arenicola(Lév.)Kers),库氏地孔菌(Geopora cooperi Harkn.),叶质地孔菌(Geopora foliacea(Schaef.)Ahmad)和梭椭孢地孔菌(Geopora perprolata B.C.Zhang,sp.nov.)。叶质地孔菌为中国新记录种。文中对各个种给予了详细的描述,并附有分种检索表。     

多年卧孔菌属五个中国新记录种

报道了多年卧孔菌属Perenniporia5个中国新记录种。非洲多年卧孔菌Perenniporia africana采自安徽省,孔口表面浅黄色至赭色,且骨架菌丝不分枝;下延多年卧孔菌P.decurrata采自云南省,担子果盖形,具较小孔口和担孢子;椭圆孢多年卧孔菌P.ellipsospora采自云南省,担子果平伏,孔口圆形至多角形,担孢子不平截;硬多年卧孔菌P.inflexibilis采自福建省,担子果盖形,孔口表面灰白至浅褐色,担孢子无色至浅黄色;黄多年卧孔菌P.xantha采自海南省,孔口较小,表面呈黄色,且在KOH试剂中呈深褐色。根据采集的标本材料提供了它们的详细描述和显微结构图。     

中国地下真菌一新记录——古氏地孔菌球孢变型

地孔菌属 Geopora中只有少数种为地下生。其中,古氏地孔菌原变型Geopora cooperi Harkness f. cooperi在我国已有报道。作者对1986年采于贺兰山的一地下真菌标本进行研究,确认该标本是古氏地孔菌球孢变型Geopora cooperi Harkness f. gilkeyae Burdsall,为我国新记录。 古氏地孔菌球孢变型 核桃仁蘑菇,图1 Geopora cooperi Harkness f. gilkeyae Burdsall, Mycologia 60: 518, 1968     

中国小木层孔菌属研究

小木层孔菌属的种类在我国共有7种,其中芳香小木层孔菌Phellinidium fragrans为中国首次报道,该种发现于吉林省长白山自然保护区,与同属其它种的区别是子实体多年生、平伏、具有芳香气味,其担孢子在同属的种类中最小,菌肉中具有形状不规则的厚垣孢子。根据中国的材料对这个种进行了详细描述和显微结构绘图。给出了该属种类的检索表,并将每种孢子统计数据列在检索表中。     

海南霸王岭自然保护区多孔菌研究(英文)

对采自海南霸王岭自然保护区的木生多孔菌约400号标本进行了鉴定共获得110种。按字母顺序列出了这些多孔菌名录;其中浅黄林氏革孔菌Lindtneria flava为中国新记录种,提供了详细描述和显微结构绘图;同时,依据文献对世界林氏革孔菌属种类进行了总结,并提供了该属所有种类的检索表。     

产于海南的中国多年卧孔菌属两新记录种

在海南省发现中国多孔菌两个新记录种,分别为戈氏多年卧孔菌Perenniporia gomezii和近邻多年卧孔菌P. vicina。根据采集的标本材料提供了它们的详细描述和显微结构图,同时提供了中国多年卧孔菌属分种检索表。     

采于海南的中国多孔菌两新记录种(英文)

在海南省发现两种多孔菌,分别为软盖革孔菌Coriolopsis byrsina和贴生硬孔菌Rigidoporus adnatus,两者均为中国新记录种。根据采集的标本材料提供了他们的详细描述和显微结构图,同时提供了中国已报道革孔菌的检索表。     

Docking Simulation and Competitive Experiments Validate the Interaction Between the 2,5-Xylidine Inhibitor and Rigidoporus lignosus Laccase

Abstract

Laccases are polyphenol oxidases which oxidize a broad range of reducing substrates, preferably phenolic compounds, and their use in biotechnological applications is increasing.

Recently, the first X-ray structure of active laccase from white rot fungus Rigidoporus lignosus has been reported containing a full complement of copper ions. Comparison among selected fungal laccases of known 3D structure has shown that the Rigidoporus lignosus laccase has a very high similarity with the Trametes versicolor laccase that, being co-crystallized with 2,5-xylidine, shows a well defined binding pocket for the substrate. Global sequence alignment between Rigidoporus lignosus and Trametes versicolor laccases shows 73% of identity but, surprisingly, there is no identity and neither conservative substitutions between the residues composing the loops directly contacting the 2,5-xylidine. Moreover the structural alignment of these two enzymes identifies in these loops a striking structural similarity proposing the question if 2,5-xylidine may bind in same enzyme pocket.

Here we report the protein-ligand docking simulation of 3D structure of Rigidoporus lignosus laccase and 2,5-xylidine. Docking simulation analyses show that spatial conformation of the two 2,5-xylidine binding pockets, despite differences in the residues directly contacting the ligand, may arrange a similar pocket that allows a comparable accommodation of the inhibitor. To validate these results the binding of 2,5-xylidine in the substrate cavity has been confirmed by kinetic competitive experiments.     

Recombinant Laccase: I. Enzyme cloning and characterization

We obtained structural and functional characterization of a recombinant Laccase from Rigidoporus lignosus (formerly Rigidoporus microporus), a white‐rot basidiomycete, by means of circular dichroism (CD) spectra, cyclic voltammetry (CV) and biochemical assays. Here we report the optimization of expression and purification procedures of a recombinant Laccase expressed in supercompetent Escherichia coli cells. We amplified the coding sequence of Laccase using PCR from cDNA and cloned into a bacterial expression system. The resulting expression plasmid, pET‐28b, was under a strong T7/Lac promoter induced by IPTG (isopropyl‐β‐d‐thiogalactoipyranoside). We obtained purification by fast protein liquid chromatography (FPLC) method. We recorded the variation of the current of a solution containing purified Laccase with increasing Syringaldazine (SGZ) concentration using a potentiometer as proof of principle, showing its compatibility with the development of a new enzymatic biosensor for medical purposes, as described in Part II. J. Cell. Biochem. 114: 599–605, 2013. © 2012 Wiley Periodicals, Inc.     

Studies in neotropical polypores 10. New polypores from Venezuela

Antrodiella dentipora, Ceriporia albobrunnea, C. cystidiata, Diplomitoporus allantosporus, D. stramineus, D. venezuelicus, Flabellophora fasciculata, Navisporus perennis, Nigroporus macroporus, Polyporus albostipes, Rigidoporus aurantiacus, Skeletocutis microcarpa, Tinctoporellus isabellinus, Trametes olivaceopora, T. supermodesta, Trichaptum variabilis, Tyromyces neostrigosus, T. polyporoides and Wrightoporia roseocontexta are described as new. Keys to all, except Trametes, the new neotropical species in their respective genera are included. The combinations Trichaptum griseofuscens (Mont.) Ryvarden & Iturriaga, and Tyromyces americanus (D. Reid) Ryvarden & Iturriaga are proposed.     

Seven Unrecorded Indigenous Fungi from Mudeungsan National Park in Korea

Fungi act as important decomposers in the forest environment. They recycle essential nutrients, promote plant growth through mycorrhizal relationships, and act as food for small animals. Samples of 265 indigenous fungal species were collected from Mudeungsan National Park in 2020. These species were identified based on morphological, molecular, and phylogenetic analyses using the internal transcribed spacer (ITS), nuclear large subunit rRNA (LSU), and RNA polymerase II second largest subunit (rpb2) regions. Subsequently, seven species were identified as unrecorded species in Korea: Cordyceps cicadae, Dentocorticium bicolor, Hymenochaete nanospora, Physisporinus crataegi, Rigidoporus piceicola, Russula raoultii, and Scutellinia crinita. This study reveals their detailed macro- and microscopic morphological characteristics with phylogenetic trees to report them as unrecorded species in Korea.     

层孔菌产漆酶的摇瓶最适培养条件研究

筛选到一株高产漆酶的层孔菌W－1,对其兴体产漆酶的培养条件进行了优化,确定了最适的碳源、氮源,并用正交试验确定了培养基中碳源和氮源的浓度。在最优化的培养条件下,培养7d后,W－1产生的漆酶活力可以达到7．1U／mL。采用补料的方法,可以得到大量的漆酶粗酶液。     

Ultrastructure of Laticifers Modifications in Hevea brasiliensis Infected with Root Rot Fungi

This paper presents some aspects of latex coagulation inside latice vessels of roots of Hevea brasiliensis infected by two fungi: Rigidoporus lignosus and Phellinus noxius. Three stages are described in latex coagulation: the phase of latex destabilisation characterized by the bursting of vacuoles and lysosomes membranes; the phase of latex coagulation characterized by the fusion of rubber particles and the disorganization of the cytoplasm; the formation of shots of rubber clumps indicating the final stage of coagulation.     

Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus

Laccase from Rigidoporus lignosus, a white-rot basidiomycete, has been isolated from culture filtrates. The enzyme was purified to homogeneity and some of its structural and kinetic parameters have been determined. The effects of pH, temperature, and organic solvents on the activity and stability of the enzyme, under different conditions, were also assayed. The results we have obtained, including the rather broad substrate specificity of enzyme, combined with their relatively easy production and purification, suggest that laccase may be efficiently employed in a variety of biotechnology applications.     

云南省柳叶藓科植物新记录

在研究标本和文献的基础上,报道了云南省柳叶藓科植物的11个新分布记录种和3个变种,包括1个中国新分布记录种细肋镰刀藓(Drepnocaldus tenuinervis Kop.),并对它们的生境和地理分布作了初步讨论．