共查询到20条相似文献,搜索用时 302 毫秒
1.
Background
Proteomic data obtained from mass spectrometry have attracted great interest for the detection of early-stage cancer. However, as mass spectrometry data are high-dimensional, identification of biomarkers is a key problem. 相似文献2.
Witold E Wolski Malcolm Farrow Anne-Katrin Emde Hans Lehrach Maciej Lalowski Knut Reinert 《Proteome science》2006,4(1):18-19
Background
The elemental composition of peptides results in formation of distinct, equidistantly spaced clusters across the mass range. The property of peptide mass clustering is used to calibrate peptide mass lists, to identify and remove non-peptide peaks and for data reduction. 相似文献3.
Background
Mass spectrometers can produce a large number of tandem mass spectra. They are unfortunately noise-contaminated. Noises can affect the quality of tandem mass spectra and thus increase the false positives and false negatives in the peptide identification. Therefore, it is appealing to develop an approach to denoising tandem mass spectra. 相似文献4.
Kouroush Sadegh Zadeh Hubert J Montas Adel Shirmohammadi 《Theoretical biology & medical modelling》2006,3(1):36-19
Background
Quantification of in-vivo biomolecule mass transport and reaction rate parameters from experimental data obtained by Fluorescence Recovery after Photobleaching (FRAP) is becoming more important. 相似文献5.
Background
Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become one of the most used tools in mass spectrometry based proteomics. Various algorithms have since been developed to automate the process for modern high-throughput LC-MS/MS experiments. 相似文献6.
Yuanyuan Wang Julie Anne Simpson Anita E Wluka Andrew J Teichtahl Dallas R English Graham G Giles Stephen Graves Flavia M Cicuttini 《Arthritis research & therapy》2009,11(2):R31
Introduction
Total joint replacement is considered a surrogate measure for symptomatic end-stage osteoarthritis. It is unknown whether the adipose mass and the distribution of adipose mass are associated with the risk of primary knee and hip replacement for osteoarthritis. The aim of the present investigation was to examine this in a cohort study. 相似文献7.
Sae-Kyung Choi In-Yang Park Jong-chul Shin 《Reproductive biology and endocrinology : RB&E》2011,9(1):6
Background
The purpose of the study was to evaluate the effects of maternal pre-pregnancy body mass index (BMI) and gestational weight gain on perinatal outcomes in a population of Korean women. 相似文献8.
A. Nur K. Hirota H. Yumoto K. Hirao D. Liu K. Takahashi K. Murakami T. Matsuo R. Shu Y. Miyake 《Journal of applied microbiology》2013,115(1):260-270
Aims
The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone‐like DNA‐binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.Methods and Results
Formed biofilm mass was measured with 0·1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7‐hydoxyl‐9H‐(1,3‐dichloro‐9,9‐dimethylacridin‐2‐one) and anti‐HLP antibody without fixation, respectively. DNase I treatment (200 U ml?1) markedly decreased biofilm formation and cell density in biofilms. Colocalization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml?1) purified from Strep. intermedius, other Gram‐positive bacteria, Gram‐negative bacteria, or human KB cells into the Strep. intermedius culture increased the biofilm mass of all tested strains of Strep. intermedius, wild‐type, HLP‐downregulated strain and control strains. In contrast, the addition of eDNA (>1 μg ml?1) decreased the biofilm mass of all Strep. intermedius strains.Conclusions
These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity.Significance and Impact of the Study
eDNA‐ and HLP‐targeting strategies may be applicable to novel treatments for bacterial biofilm‐related infectious diseases. 相似文献9.
Background
Overweight is reported as a side effect of SDR. The aims were to study the development of weight, height and body mass index (BMI) during five years after SDR. 相似文献10.
Background
Peptide Mass Fingerprinting (PMF) is a widely used mass spectrometry (MS) method of analysis of proteins and peptides. It relies on the comparison between experimentally determined and theoretical mass spectra. The PMF process requires calibration, usually performed with external or internal calibrants of known molecular masses. 相似文献11.
Towards the systematic discovery of signal transduction networks using phosphorylation dynamics data
Haruna Imamura Nozomu Yachie Rintaro Saito Yasushi Ishihama Masaru Tomita 《BMC bioinformatics》2010,11(1):232
Background
Phosphorylation is a ubiquitous and fundamental regulatory mechanism that controls signal transduction in living cells. The number of identified phosphoproteins and their phosphosites is rapidly increasing as a result of recent mass spectrometry-based approaches. 相似文献12.
Grazyna?Kwapiszewska Markus?Meyer Ralf?Bogumil Rainer?M?Bohle Werner?Seeger Norbert?Weissmann Ludger?Fink
Background
Laser microdissection allows precise isolation of specific cell types and compartments from complex tissues. To analyse proteins from small cell numbers, we combine laser-microdissection and manipulation (LMM) with mass spectrometry techniques. 相似文献13.
Background
Decoding of the liver proteome is subject of intense research, but hampered by methodological constraints. We recently developed an improved protocol for studying rat liver proteins based on 2-DE-MALDI-TOF-MS peptide mass finger printing. 相似文献14.
Background
The scope of this work is to show that the correct and complete definition of the system of muscle contraction requires the knowledge of both the mass and the acceleration of the load. 相似文献15.
Background
The relationship between body mass (M) and standard metabolic rate (B) among living organisms remains controversial, though it is widely accepted that in many cases B is approximately proportional to the three-quarters power of M. 相似文献16.
Background
New approaches are needed for large-scale predictive modeling of cellular signaling networks. While mass action and enzyme kinetic approaches require extensive biochemical data, current logic-based approaches are used primarily for qualitative predictions and have lacked direct quantitative comparison with biochemical models. 相似文献17.
Background
The development of high-throughput technologies such as yeast two-hybrid systems and mass spectrometry technologies has made it possible to generate large protein-protein interaction (PPI) datasets. Mining these datasets for underlying biological knowledge has, however, remained a challenge. 相似文献18.
Zhuo Zhang Shiwei Sun Xiaopeng Zhu Suhua Chang Xiaofei Liu Chungong Yu Dongbo Bu Runsheng Chen 《BMC bioinformatics》2006,7(1):222-8
Background
Tandem mass spectrometry (MS/MS) is a powerful tool for protein identification. Although great efforts have been made in scoring the correlation between tandem mass spectra and an amino acid sequence database, improvements could be made in three aspects, including characterization ofpeaks in spectra, adoption of effective scoring functions and access to thereliability of matching between peptides and spectra. 相似文献19.
Background
Mass spectrometry-based biomarker discovery has long been hampered by the difficulty in reconciling lists of discriminatory peaks identified by different laboratories for the same diseases studied. We describe a multi-statistical analysis procedure that combines several independent computational methods. This approach capitalizes on the strengths of each to analyze the same high-resolution mass spectral data set to discover consensus differential mass peaks that should be robust biomarkers for distinguishing between disease states. 相似文献20.