共查询到20条相似文献,搜索用时 15 毫秒
1.
The red coral grouper, Plectropomus maculatus, is a high value marine food fish species. We isolated the first set of 10 polymorphic microsatellites and examined their polymorphism in two related species: the Malabar grouper Epinephelus malabaricus and the brown‐marbled grouper Epinephelus fuscoguttatus. The average allele number of these 10 microsatellite DNA loci is 9.1/locus with a range of four to 17, whereas the expected heterozygosity ranged from 0.18 to 0.94, averaging 0.76. Five of the 10 markers amplified polymorphic and specific products in E. malabaricus, whereas only four were amplified in E. fuscoguttatus. These microsatellites could be applicable to captive breeding and to the studies on genetic diversity and population structure of grouper wild stocks. 相似文献
2.
Chinese mitten crab (Eriocheir sinensis) has great commercial significance in Asia, but is an invasive organism in Europe and America. We isolated five novel microsatellites for E. sinensis. All five loci were polymorphic. The average allele number was 16.8 per locus with a range of 13 to 21, while the expected heterozygosity ranged from 0.82 to 0.91 with an average of 0.88. These markers could be applicable to studies of the genetic diversity and population structure of E. sinensis. 相似文献
3.
Ramesh K. Aggarwal Albert Lalremruata Thirumalaisamy P. Velavan Ayyadevara Pavani Sowjanya Lalji Singh 《Conservation Genetics》2008,9(4):981-984
Olive ridleys, one of the widely distributed marine turtle species has undergone declines in recent years due to multiple
anthropogenic factors warranting conservation efforts for which assessment of genetic variability in existing populations
become critical. Here we describe development of ten new microsatellite markers from a short sequence repeat-enriched partial
genomic DNA library, which are found to be highly informative for genetic studies. Eight of these markers when tested on 83
olive ridley turtles revealed high allelic diversity (4–27 alleles per marker), and high observed and expected heterozygosity
estimates that ranged from 0.29 to 0.82 and 0.62 to 0.94, respectively. Two microsatellites were monomorphic in the tested
olive ridley samples, but were found to be informative/polymorphic when tested on related marine turtle species. More importantly,
nine of the new markers showed robust cross-species amplifications in three related species Dermochelys coriacea, Chelonia mydas and Eretmochelys imbricata. Thus, this study describes ten new microsatellite markers and also demonstrates their potential as efficient genetic markers
in studies related to parentage analysis, population structure, phylogeography and species relationships of olive ridleys
and other marine turtle species. 相似文献
4.
We isolated 12 polymorphic microsatellites from an important marine food fish Larimichthys polyactis and characterized them in 32 unrelated individuals. Among the 12 microsatellites, four were tetranucleotide repeats and eight were dinucleotide repeats. The allele number ranged from five to 25 with an average of 15.4/locus; average expected heterozygosity was 0.81, ranging from 0.57 to 0.95, whereas the observed heterozygosity ranged from 0.34 to 1.00 (average: 0.78). Nine of the 12 markers conformed to Hardy–Weinberg equilibrium and showed no sign of linkage. These microsatellites will be useful for population genetic studies and selective breeding programs of this species. 相似文献
5.
Brian M. Shamblin Brant C. Faircloth Mark G. Dodd Dean A. Bagley Llewellyn M. Ehrhart Peter H. Dutton Amy Frey Campbell J. Nairn 《Conservation Genetics》2009,10(3):577-580
The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and
polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea
turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded
an average of 11.2 alleles per locus (range of 4–24), an average observed heterozygosity of 0.83 (range 0.59–0.96), and an
average polymorphic information content of 0.80 (range 0.62–0.94). We also demonstrate the utility of these primers, in addition
to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the
two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea). 相似文献
6.
The ribbonfish, Trichiurus haumela, is an important marine foodfish species. Supply of this fish relies on capture from the wild. No information about genetic diversity and population structure of wild stocks is available. Therefore, we developed the first set of 10 polymorphic microsatellites. The average allele number was 13.2 per locus ranging from six to 20. The expected heterozygosity was 0.84, whereas the observed heterozygosity was 0.71. This set of microsatellites provides a tool for assessing genetic diversity and population structure of this species. 相似文献
7.
J. CHAIB S. DANAN B. JOUAUD L. S. HAGEN F. LEFEVRE B. FADY 《Molecular ecology resources》2006,6(3):840-842
We developed primers for the amplification of six polymorphic nuclear microsatellites in Mediterranean Cedrus taxa. Microsatellites originated from two Cedrus atlantica genomic libraries enriched for TC (four markers) and TG (two markers) motifs. No distortion from Mendelian segregation was observed. There were up to eight alleles per locus and mean expected heterozygosity was 0.75. Four microsatellites also amplified in the Himalayan cedar. These markers should be helpful for species identification, diversity studies, parentage analysis and genome mapping and to monitor biodiversity in endangered Mediterranean Cedrus forests. 相似文献
8.
Crimson red snapper (Lutjanus erythropterus) is an important marine foodfish species in Asia with great potentials for aquaculture. We isolated nine polymorphic microsatellites, among which eight were tetranucleotide repeats, and one was CA‐microsatellite. The average allele number present in 48 individuals was 11.1/locus, ranging from three to 33. The expected heterozygosity ranged from 0.49 to 0.97 with an average of 0.74. Six of the nine markers conformed to the Hardy–Weinberg expectations. No pairwise markers showed the possibility of linkage. Protocols for two multiplex polymerase chain reactions (PCRs), each amplifying two and seven markers, respectively, were presented. The developed microsatellites and optimized multiplex PCRs will be useful for studying population structure of wild stocks and parentage assignment for cultured populations. 相似文献
9.
Fourteen polymorphic microsatellite loci were isolated from the golden pheasant (Chrysolophus pictus). The average allele number of these microsatellites was 11.14 per locus, ranging from two to seventeen in a group of 31
individuals. The mean observed and expected heterozygosities were 0.763 and 0.722, respectively. The average polymorphic information
content value, cumulative discrimination power, overall probability of exclusion with both unknown parents or with only unknown
sire for these markers were 0.741, 1.0, 0.9999, and 1.0, respectively. This set of microsatellite markers would provide useful
tools for conservation genetic studies of the golden pheasant. 相似文献
10.
Nineteen polymorphic microsatellite markers were isolated and characterized from a trinucleotide enriched partial genomic library of Epimedium koreanum. The average allele number of these microsatellites was 5.9 per locus, ranging from two to 11. The observed heterozygosity (HO) and expected heterozygosity (HE) at the population level were 0.00–0.90 and 0.12–0.90, respectively. In addition, the results of cross‐species amplification of this set of microsatellite markers in four closely related Epimedium species, E. brevicornum, E. sagittatum, E. pubescens and E. wushanense, revealed that these microsatellite markers were useful for population genetic structure evaluation and genotype analysis of major Epimedium species that have been used as traditional Chinese medicines. 相似文献
11.
Twelve polymorphic microsatellite markers were isolated from an AC-enriched genomic library of Buff-throated partridge (Tetraophasis szechenyii). The allele number of these loci ranged from three to 13 (average 7.75 per locus) in tested individuals. Polymorphism information
content ranged from 0.532 to 0.882 with an average of 0.721. Average observed and expected heterozygosities were 0.559 (range
from 0.240 to 0.792) and 0.772 (range from 0.621 to 0.910), respectively. These microsatellite markers will be useful for
the assessment of genetic diversity, relatedness identification of family and analysis of population structure in wild buff-throated
partridge. 相似文献
12.
Junco Nagata Youichi Sonoda Keiko Hamaguchi Naoki Ohnishi Soh Kobayashi Ken Sugimura Fumio Yamada 《Conservation Genetics》2009,10(4):1121-1123
We report on the isolation and characterization of eight polymorphic and five monomorphic microsatellites in the Amami rabbit
(Pentalagus furnessi). Microsatellite polymorphism was determined using 25 individuals. There were 2–11 alleles for each polymorphic locus with
heterozygosity ranging between 0.08 and 0.76. Linkage disequilibrium was not suggested between any pairs among the eight polymorphic
loci. We suggest that these primers be used in future studies to monitor population size, determine dispersal patterns, and
genetic diversity within and between populations of this and related species. 相似文献
13.
We developed microsatellite markers in American yam (Dioscorea trifida). A microsatellite sequence‐enriched genomic library was screened, and after sequencing, primers were designed for 20 microsatellites. Among these, eight primer pairs yielded amplification products that were both interpretable and polymorphic in 24 yam cultivars. The number of alleles per locus ranged from two to 13 and the overall expected heterozygosity was around 0.5. Six of the eight Dioscorea trifida microsatellite loci gave amplification products in other Dioscorea species. 相似文献
14.
XIAOHONG YAO QIGANG YE MING KANG JIANFENG ZHOU YANQIN XU YING WANG HONGWEN HUANG 《Molecular ecology resources》2006,6(1):133-136
Twenty‐four polymorphic microsatellite loci were isolated and characterized from an AAG‐enriched genomic library of Sinojackia xylocarpa. The average allele number of these microsatellites was 3.3 per locus, ranging from two to seven. The observed and expected heterozygosities at population level were 0.10–0.83 and 0.10–1.00, respectively. In addition, successful cross‐species amplification of this set of microsatellites in three other species of Sinojackia and a closely related taxon, Changiostyrax dolichocarpa, suggested that this set of microsatellite markers should provide a useful tool for genetic and conservation studies of Sinojackia species and other closely related taxa in the Styracaceae. 相似文献
15.
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, we developed 11 novel microsatellite loci for this rare
species and applied them to examine genetic variation of indigenous alligators from Changxing Nature Reserve and America-born
Chinese alligators. The 11 polymorphic microsatellites presented a total of 31 alleles among 57 individuals scored, yielding
an average of 2.82 alleles per locus. One allele was unique to the American population but four private alleles were detected
in the Changxing population. The average expected and observed heterozygosities were 0.400 and 0.482 for the Changxing alligators
and 0.520 and 0.621 for the America-born individuals, respectively. These microsatellite markers would be useful tools in
the genetic examination of this endangered species. 相似文献
16.
The first set of six polymorphic microsatellites was isolated from a partial genomic DNA library of an important marine food fish Gnathanodon specious, and was characterized in 28 unrelated individuals. The allele number ranged from three to 12 with an average of seven/locus; average expected heterozygosity was 0.57 ranging from 0.10 to 0.87, whereas the observed heterozygosity ranged from 0.11 to 0.96 (average: 0.57). All six markers conform to Hardy–Weinberg and linkage equilibria. These markers will be useful for accessing genetic population structure of wild populations and for selective breeding program in fish farms. 相似文献
17.
Characterization of microsatellites located within the genes of goldfish (Carassius auratus auratus)
The goldfish (Carassius auratus auratus; Cyprinidae) is not only an important ornamental fish species, but also a useful model for biological studies. The sequence of goldfish genes present in the public database was searched for short tandem repeats, and 11 polymorphic microsatellites were detected within eight important genes. Two microsatellites were located in coding regions of the c‐myc and GAP‐43 genes, respectively, whereas the others were mainly located in 5′ and 3′ untranscribed regions of other genes, such as gonadotropin and activin. The average allele number of these microsatellites was 5.5 per locus with a range between 3 and 9. These microsatellites will be useful for ecological and population genetic studies of this species, as well as for the ornamental fish industry. 相似文献
18.
Novel polymorphic microsatellites for studying genetic diversity of red Asian arowanas 总被引:1,自引:0,他引:1
Asian arowana (Scleropages formosus) is a highly endangered fish species listed in Appendix 1 of CITES since 1980. Fourteen novel polymorphic microsatellites were isolated from a CA-enriched partial genomic DNA library, and were used for studying genetic diversity of 41 red arowanas from the wild population. Surprisingly the average allele number of the 14 polymorphic microsatellites was as high as 12.4/locus. The average observed heterozygosity was 0.78 ranging from 0.51 to 0.95, and the gene diversity was quite high (0.78). All these data suggest that high level of genetic diversity existed in the red Asian arowana population. 相似文献
19.
JIA LE LI ZE YUAN ZHU GUI LING WANG ZHI YI BAI GEN HUA YUE 《Molecular ecology resources》2007,7(6):1114-1116
Here we report the isolation and characterization of 17 polymorphic loci isolated from a partial genomic DNA library of grass carp (Ctenopharyngodon idellus) enriched for CA repeats. We tested variability of these microsatellites on 24 unrelated individuals collected in China. All microsatellites were polymorphic. The average allele number was 7.9 per locus, ranging from four to 13. The observed heterozygosity was from 0.46 to 0.88 with an average of 0.71, whereas the average expected heterozygosity was 0.78. Sixteen of the 17 microsatellites conformed to Hardy–Weinberg equilibrium, and inherited independently. These microsatellites can be used to study genetic diversity and population structure of wild populations, and facilitate selective breeding of cultured broodstocks. 相似文献
20.
The yellow catfish Pelteobagrus fulvidraco is a freshwater fish species. Due to overfishing and pollution of freshwater ecosystems, the wild stocks of this fish reduced
substantially. We isolated and characterized 12 polymorphic microsatellites of this species. The number of alleles at the
12 microsatellite loci ranged from four to eight, with an average of 6.6/locus. The average observed heterozygosity was 0.72,
whereas the expected heterozygosity ranged from 0.60 to 0.86 (average: 0.80). All 12 microsatellites conformed to Hardy–Weinberg
Equilibrium and were in linkage equilibrium. These 12 novel microsatellites could facilitate studies of genetic diversity
and population structure of the yellow catfish to supply necessary information of conservation of the yellow catfish. 相似文献