杀虫双对梭毒隐翅虫捕食功能的影响

梭毒隐翅虫Paederus fuscipes Curtis是一种重要的天敌昆虫.在室内条件下,采用浸渍法测定了18%高效杀虫双在田间常用最低浓度时,对梭毒隐翅虫成虫死亡率和捕食功能的影响.试验结果表明,18%高效杀虫双对梭毒隐翅虫的致死作用及捕食功能影响均较小,但对残存个体捕食功能的影响超过致死作用.     

几种农药对梭毒隐翅虫的毒力测定

梭毒隐翅虫Paederus fuscipesCurtis是农田常见的捕食性天敌昆虫。在室内条件下采用浸渍法分别测定了5种常用杀虫农药对梭毒隐翅虫成虫的杀伤情况。试验结果表明:10%毒.溴乳油对梭毒隐翅虫的毒力最强,40%氧化乐果乳油毒力次之,22%噻.吡可湿性粉剂和50%辛硫磷+氰戊菊酯毒力较弱,18%杀虫双水剂(bisultap)毒力最弱。     

苦参碱对大眼隐翅虫和梭毒隐翅虫的影响

大眼隐翅虫Stenus (Stenus,s.str)sp.1和梭毒隐翅虫Paedenus fusipes Curtis是重要的天敌昆虫。用苦参碱在室内做了大眼隐翅虫和梭毒隐翅虫的药效实验。结果表明,苦参碱对大眼隐翅虫和梭毒隐翅虫有一定的毒杀作用。     

梭毒隐翅虫人工饲料研究

分别用一种天然饲料和三种人工饲料以同样的方法喂养梭毒隐翅虫(Paederus fuscipes Curtis)成虫,结果表明四种饲料都能使梭毒隐翅虫存活,就可操作性而言猪肝粉蜂蜜混合饲料优于其他三种饲料,可加工成成品饲料饲养与梭毒隐翅虫食性相近的毒隐翅虫成虫.     

Bt水稻对青翅蚁形隐翅虫存活及捕食功能的影响

【目的】以稻田中重要捕食性天敌青翅蚁形隐翅虫Paederus fuscipes Curtis为研究对象,探讨在室内条件下青翅蚁形隐翅虫取食转Bt基因水稻上的褐飞虱后对其存活率和捕食功能的影响。【方法】在室内条件下,取食通过用一直取食转Bt水稻的褐飞虱Nilaparvata lugens(St?l)来饲养青翅蚁形隐翅虫,在第7、14、21、28天调查其成活率,研究转Bt水稻对青翅蚁形隐翅虫生长的影响。通过设置不同的猎物密度和捕食者密度,研究转Bt水稻对青翅蚁形隐翅虫成虫捕食功能的影响。【结果】室内条件下,转Bt水稻对青翅蚁形隐翅虫的存活率无明显影响。在试验褐飞虱密度下,转Bt基因水稻对青翅蚁形隐翅虫对褐飞虱的捕食量无显著影响,且捕食作用方程可用HollingⅡ模型拟合,理论方程为Na=1.3421N/(1+0.0887N)。瞬时攻击率a′和平均处理时间Th无显著差异,同时,转Bt基因水稻对青翅蚁形隐翅虫的捕食干扰反应无显著影响。【结论】转Bt基因水稻对供试天敌昆虫青翅蚁形隐翅虫存活率和捕食功能无明显影响。     

两种杀虫剂对稻田捕食性天敌集团捕食功能的影响

将稻田各类捕食性天敌作为一集团,运用功能法评价了甲胺磷和杀虫双对该集团捕食功能的影响,由于各类捕食性天敌对杀虫剂敏感性的不同,甲胺磷和杀虫双造成的各类天敌的死亡率和残存天敌个体的捕食功能减退率均不同。实验表明,隐翅虫类和狼蛛类对甲胺磷较为敏感,而对杀虫双,肖蛸类最为敏感。杀虫双对隐翅虫类的致死作用及捕食功能影响较小。就集团的捕食功能而言,甲胺磷影响明显大于杀虫双,但在总的功能减退率组分中,甲胺磷致     

三种隐翅虫下颚须和下唇须感器超微结构的比较研究(鞘翅目:隐翅虫总科)

利用扫描电子显微镜对3种隐翅虫成虫的下颚须和下唇须外部形态进行观察,结果表明,3种隐翅虫成虫的下颚须、下唇须末节感器变化较大。梭毒隐翅虫Paederus fuscipes Curtis下颚须末节有毛形感器、刚毛形感器、柱形感器、感器簇和板形感器,其中柱形感器、感器簇和板形感器在其它昆虫下颚须中未见报道,下唇须末节有锥形感器。闪蓝束毛隐翅虫Dianous coeruleotinctus Puthz和瘦突眼隐翅虫Stenus tenuipes Sharp下颚须、下唇须末节较光滑,仅闪蓝束毛隐翅虫下颚须末节有孔状结构。此外,文中还探讨了这些感器的功能及在分类学上的意义。     

稻田主要捕食性天敌的栖境生态位与捕食作用分析

本文进行了稻田捕食性天敌与稻飞虱的栖境生态位调查,并应用正交试验设计、添加试验法和排除试验法研究了?物种的栖境生态位与各天敌捕食作用的关系,结果表明：在5种灭敌与2种害虫共存系统中,天敌对褐飞虱Nilaparvata lugens Stal捕食量的正主效应大小依次为： 粽管巢蛛 Clubiona aponicola、青翅蚁形隐翅虫Paederus fuscipes、前齿肖蛸Tetragnatha praedonia、食虫沟瘤蛛 Ummeliata insecticeps,拟水狼蛛Pirata subpiraticus的负主效应值最大(随狼蛛密度增大捕食量下降);对稻纵卷叶螟Cnaphlocrcis medinalis Guenee捕食量正主效应大小依次为： 粽管巢蛛、肖蛸、青翅蚁形隐翊虫。多物种共存系统中,狼蛛、微蛛、跳蛛主要捕食飞虱;肖蛸主要捕食稻纵卷叶螟;管巢蛛对两种害虫均具有较好的控制作用。这与各物种的生态位宽度值相一致。当飞虱密度在每穴10一30头时,多物种共存系统优良的生物序是拟水狼蛛、食虫瘤胸蛛、青翅蚁形隐翅虫各为l头,棕管巢蛛2头,此时天敌的控制作用最大。添加法和排除法试验结果表明在具-定数量的多物种共存系统中,再增加天敌密度不一定能增加猎物的被捕食数,其直接的生物学原因是种间种内干扰(包括种间种内残杀)     

氟虫腈、吡虫啉作为黑翅土白蚁诱杀药剂的效果

毒力测定结果表明,0.025~0.4μg/mL氟虫腈和吡虫啉分别在药后3 d和5 d对黑翅土白蚁Odontotermes formosanus表现出明显的毒杀效果,氟虫腈和吡虫啉药后1 d的LC50分别为药后5 d的509倍和63.8倍,2种药剂对黑翅土白蚁的毒杀效果均比较缓慢。毒性传递试验表明,0.5μg/g毒沙处理白蚁1 h后,氟虫腈和吡虫啉的致死毒性均可被传毒白蚁传递给受毒白蚁。驱避作用试验表明,50μg/mL氟虫腈对黑翅土白蚁无明显的驱避作用,而50μg/mL吡虫啉对黑翅土白蚁表现出了明显的驱避作用。可见,2种供试药剂中,氟虫腈是较理想的白蚁诱杀药剂。     

青翅蚁形隐翅虫对稻纵卷叶螟和白背飞虱的捕食作用

应用二次回归正交设计建立青翅蚁形隐翅虫对稻纵卷叶螟和白背飞虱捕食作用的数学模型分别为：Y＇cm=3.5072＋1.6671x1＋1.7200x2-1.004x3＋0.6375x1x2-0.7625x1x3-0.2625x2x3＋0.6020x^21-0.3538x^22-0.0807x^23;Y＇sf=3.7612＋0.4015x1＋0.2573x2＋1.6645x3＋0.1250x1x3＋x2x3＋0.3372x^21-0.4820x^22＋0.0641x^23.结果表明,青翅蚁形隐翅虫对白背飞虱表现出更强的取食选择性.当猎物密度较低时,青翅蚁形隐翅虫对稻纵卷叶螟的捕食作用为密度制约性,当猎物密度较高时,青翅隐翅虫对白背飞虱的捕食作用为密度制约性.     

Does pollen availability mitigate the impact of pesticides on generalist predatory mites?

The effect of the provision of pollen on the impact of pesticides on the predatory mite Kampimodromus aberrans was assessed at individual and population levels. In the laboratory we evaluated the influence of pollen amount and pollen application frequency on lethal and sub-lethal effects of chlorpyrifos and spinosad. In a potted plant experiment, the effects of pesticides and pollen were assessed on predatory mite population abundance. In the laboratory, survival and fecundity of predatory mites were reduced by insecticides, and spinosad was more toxic than chlorpyrifos. In the same experiment, high pollen application frequency alleviated the sub-lethal effect induced by chlorpyrifos. On potted plants, pollen applications reduced the impact of chlorpyrifos on K. aberrans, whereas without pollen applications the impact of spinosad and chlorpyrifos on the predatory mite population was similar. Results obtained here highlight that the provision of fresh pollen is of particular importance for predatory mites when pesticides are applied.     

The impact of insecticides applied in apple orchards on the predatory mite Kampimodromus aberrans (Acari: Phytoseiidae)

Kampimodromus aberrans is an effective predatory mite in fruit orchards. The side-effects of insecticides on this species have been little studied. Field and laboratory experiments were conducted to evaluate the effects of insecticides on K. aberrans. Field experiments showed the detrimental effects of etofenprox, tau-fluvalinate and spinosad on predatory mites. Spider mite (Panonychus ulmi) populations reached higher densities on plots treated with etofenprox and tau-fluvalinate than in the other treatments. Single or multiple applications of neonicotinoids caused no detrimental effects on predatory mites. In the laboratory, spinosad and tau-fluvalinate caused 100 % mortality. Etofenprox caused a significant mortality and reduced fecundity. The remaining insecticides did not affect female survival except for imidacloprid. Thiamethoxam, clothianidin, thiacloprid, chlorpyrifos, lufenuron and methoxyfenozide were associated with a significant reduction in fecundity. No effect on fecundity was found for indoxacarb or acetamiprid. Escape rate of K. aberrans in laboratory was relatively high for etofenprox and spinosad, and to a lesser extent thiacloprid. The use of etofenprox, tau-fluvalinate and spinosad was detrimental for K. aberrans and the first two insecticides induced spider mite population increases. The remaining insecticides caused no negative effects on predatory mites in field trials. Some of them (reduced fecundity and repellence) should be considered with caution in integrated pest management programs.     

用ELISA方法研究稻田节肢动物的食物关系

应用酶联免疫吸附试验双抗体夹心法,研究了稻田节肢动物,包括19种捕食性天敌、4种主要水稻害虫及1种中性昆虫之间的食物关系。在检测的19种捕食者中,有15种捕食了白背飞虱,11种捕食了褐飞虱。其中食虫沟瘤蛛、拟水狼蛛、八斑鞘蛛、粽管巢蛛、四斑锯螯蛛和拟环纹豹蛛对两种稻飞虱的捕食阳性率较高。有7种捕食了稻纵卷叶螟幼虫,其中粽管巢蛛、褶管巢蛛、拟环纹豹蛛、青翅蚁形隐翅虫和印度细颈步甲的捕食阳性率较高。拟环纹豹蛛、浙江豹蛛和拟水狼蛛对稻蝗若虫有较高的阳性率。13种捕食者捕食了摇蚊,摇蚊作为替代或补充猎物,对稻田捕食性节肢动物亚群落的重建和发展具有重要意义。在实验检测的基础上,构建了被检测节肢动物猎物与捕食者间的食物关系图。     

三种农药亚致死剂量对椰甲截脉姬小蜂功能反应的影响

测定了氯氰菊酯、啶虫脒和椰甲清3种杀虫剂亚致死剂量对椰甲截脉姬小蜂Asecodes hispinarumBoucˇek寄生椰心叶甲Brontispa logissima(Gestro)功能反应的影响。结果表明:亚致死剂量杀虫剂没有改变椰甲截脉姬小蜂功能反应模型的基本结构,但影响到了模型的各项参数。药剂处理后椰甲截脉姬小蜂的最大日捕食量降低,处理猎物时间延长,搜寻效应减弱。氯氰菊酯和啶虫脒的处理后瞬时攻击率减小,但椰甲清处理后瞬时攻击率高于对照。可见,在亚致死剂量下,氯氰菊酯和啶虫脒对椰甲截脉姬小蜂的寄生作用存在不良影响,椰甲清(30%杀虫单+15%啶虫脒)可提高其瞬时攻击率但减弱其搜索效应。     

Effects of reduced rates of two insecticides on enzyme activity and mortality of an aphid and its lacewing predator

By applying insecticides at lower rates of active ingredients per unit area, survival rates of the pests' natural enemies can be enhanced, whereas pest mortality can remain high. The effects of reduced application rates of the insecticides lambda-cyhalothrin and dimethoate on the mortality of bird cherry-oat aphid, Rhopalosiphon padi (L.), and lacewing Micromus tasmaniae Walker were determined in the laboratory and field. Cholinesterase (ChE) and glutathione S-transferase (GST) activities in survivors provided a measure of sublethal effects and general fitness. In the laboratory, lacewings were less sensitive than aphids to both insecticides, and dimethoate was more toxic than lambda-cyhalothrin. However, these results could not be recreated in the field, in part due to very low recapture rates. In summary, lambda-cyhalothrin seemed to have no effect on aphids, but it was toxic to lacewings. Dimethoate was far less toxic in the field, but aphids were still more sensitive than were lacewings. Cholinesterase activity was reduced by dimethoate exposure in the laboratory in both species, but there were species-specific differences. Dimethoate and lambda-cyhalothrin had no effects on GST activity in either species. The high mortality rate for lacewings and aphids exposed to dimethoate in the field suggests that the application rate could be reduced to as low as 10% of that recommended by manufacturers, and this should still be highly efficacious against aphids, while protecting the predatory lacewing. Measurement of enzyme activity could provide a useful indicator of "fitness" of survivors.     

温度对中华锉叶蜂发育和存活的影响及药剂防治效果

中华锉叶蜂Pristiphora sinensisWong是我国特有的害虫种类。在室内较系统地研究了温度对中华锉叶蜂发育和存活的影响,并进行了田间防治试验。结果表明,不同温度对中华锉叶蜂各虫态生长发育和存活具有明显不同的影响,在16,20,24,28℃条件下,卵、幼虫、蛹的存活率和发育历期均存在显著的差异,比较而言,以20~24℃较为适宜,温度过高或过低均有明显不利的影响。药剂防治试验表明,供试6种药剂处理对中华锉叶蜂幼虫均具有较好的防治效果。     

The toxic effect of greenhouse insecticides on the predatory bugs <Emphasis Type="Italic">Nesidiocoris tenuis</Emphasis> Reuter and <Emphasis Type="Italic">Macrolophus pygmaeus</Emphasis> H.-S. (Heteroptera,Miridae)

The study reports the results of toxicity tests for several insecticides which are allowed to be used on greenhouse plants (Admiral, Mospilan, Spintor, and BT), as applied to different developmental stages of the predatory bugs Nesidiocoris tenuis Reuter and Macrolophus pygmaeus H.-S. (family Miridae), which feed on whiteflies, aphids, thrips, and spider mites. The juvenoid Admiral is found to be the least toxic to these species, whereas the spinosyn Spintor is most toxic. The neonicotinoid Mospilan and a microbiological insecticide BT are intermediate in their effects. The timing of application of insecticides most favorable to the predatory mirids when both pest management methods are used together is discussed.