共查询到20条相似文献,搜索用时 62 毫秒
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天花粉蛋白(TCS)在≥2.22μmol/L时能引起大豆磷脂脂质体内含物释放,Ca^2+对这种释放有一定的促进作用,低PH值也能促进TCS与脂质体的作用;将TCS与细细胞一起保温;当TCS终浓度达14.7μmol/L时能损伤红细胞膜,产生溶血作用;TCS还能作用于红细胞血影膜,改变其脂双层的不对称性。 相似文献
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多细胞有机体中每个细胞的活动不是孤立进行的,在众多细胞之间存在着一套复杂的跨膜信号传递系统(Transmembranesignaltransd-uctionsystem),使细胞间能相互联系和沟通,协调不同组织器官中各个细胞的代谢、分泌、分化和增殖等... 相似文献
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脱血红素细胞色素c与膜结合及插膜时的构象研究 总被引:1,自引:0,他引:1
应用特殊的单分子层样品制备技术,分别制备了与中性、酸性磷脂膜结合的和完全插膜的鸡心脱血红素细胞色素c样品,并运用圆二色谱(CD)、表面衰减全反射Fourier变换红外光谱(ATR-FTIR)对膜上蛋白的构象进行了鉴定.研究结果表明,蛋白在与膜结合及插入阶段的构象是不同的,膜界面性质的不同也会对蛋白的构象产生不同的诱导,在酸性磷脂DSPG膜表面,该蛋白是以α螺旋和β折叠混合的构象形式结合;而在中性磷脂DSPC膜表面是以β折叠为主的构象形式结合.插入 DOPG单分子层内时则是 α螺旋为主的构象形式. 相似文献
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以‘日本晴’水稻为试验材料,研究铝胁迫对水稻幼苗生长的影响,及铝胁迫下其根系和功能叶片中脂质含量及组分的变化。结果表明Al处理显著抑制了水稻根尖的伸长和生长,降低了植株的生物量以及叶绿素含量。铝胁迫后,半乳糖脂含量变化显著,根和叶中的单半乳糖甘油二酯(MGDG)和双半乳糖甘油二酯(DGDG)含量均降低;而磷脂(PL)及硫代异鼠李糖甘油二酯(SQDG)的含量在根中表现为上升,在叶中则显著降低。此外,根和叶中的脂肪酸不饱和度均降低。由此可见,铝胁迫引起水稻膜脂组分和含量的变化,这种变化可能是铝抑制植物生长的重要原因之一。 相似文献
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THE FINE STRUCTURE AND IMMUNOLOGICAL LABELING OF THE ACHROMATIC MITOTIC APPARATUS AFTER DISRUPTION OF CELL MEMBRANES 总被引:3,自引:1,他引:2 下载免费PDF全文
After treatment of HeLa and L cells with vinblastine sulfate the material of microtubules (tubulin) was reorganized into (a) large paracrystals (PC) of tightly packed tubules; (b) smaller aggregates of tubules with greater diameter whose walls are constituted from well defined, helically arranged morphological subunits; and (c) microtubules associated with helices of polyribosomes of uniform size. All of these structures survived disruption of cellular membranes by means of a nonionic detergent. Following a thorough stripping of membranes there remained a subcellular fraction sedimenting at 1,500 g for 15 min, in which were contained nuclei, centrioles, and the above mentioned microtubular elements, maintained as a complex of organelles by an interconnecting network of 80 Å microfibrils. As a result of membrane disruption it was possible to localize precisely in the electron microscope the binding of ferritin antibody conjugates. Specific labeling at the surface of PC and microtubule aggregates could be demonstrated. This result was substantiated by means of the immunoperoxidase method of labeling the PC. A concentrated deposit of ferritin was also found in the vicinity of centrioles and related structures, the annuli of the nuclear pore complex and the annulate lamellae. However, the specificity of the label on these organelles remains questionable because ferritin, albeit in lower concentration, was also present on them in control preparations reacted with preimmune sera. 相似文献
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1. Experiments were carried out to decide whether or not the electromotive properties of dried collodion membranes depend upon their thickness. 2. A number of dried collodion membranes of varying thickness, 3–160 µ, were prepared from collodion preparations of different electrochemical activity. The characteristic concentration potentials across them were measured and the means of these values determined for each thickness. 3. The characteristic concentration potentials across dried collodion membranes are a function of their thickness. The thinnest membranes yield in all cases the lowest concentration potentials; increasingly thicker membranes give increasingly higher potential values, until a constant value is reached which is characteristic of the particular collodion preparation used. With electrochemically active collodion, characteristic concentration potentials approaching the thermodynamically possible maximum are obtained with membranes of only 10 µ thickness, thinner membranes giving appreciably lower values. With two rather inactive commercial collodion preparations the characteristic concentration potential increases from about 30 mv. for membranes 3 µ thick to about 42 mv. for 20 µ membranes; still thicker membranes do not show a significant increase in the potential values. With a highly purified collodion preparation the constant maximum value was found to be about 32 mv., 4 µ thick membranes giving only about 22 mv. 4. These results do not support the homogeneous phase theory as applied to the dried collodion membrane. They are readily compatible with the micellar-structural theory. Several special possible cases of the latter as applied to the dried collodion membrane are discussed. 相似文献
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Marian Irwin 《The Journal of general physiology》1923,5(4):427-428
1. A method is given for determining the chloride content in a drop (less than 0.03 cc.) of the cell sap of Nitella. 2. Chlorides accumulate in the sap to the extent of 0.128 M; this accumulation can be followed during the growth of the cell. The chloride content does not increase when the cell is placed for 2 days in solutions (at pH 6.2) containing chlorides up to 0.128 M. 3. The exosmosis of chlorides from injured cells can be followed quantitatively. When one end of the cell is cut off a wave of injury progresses toward the other end; this is accompanied by a progressive exosmosis of chlorides. 相似文献
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ON THE MECHANISM OF TAURINE TRANSPORT AT BRAIN CELL MEMBRANES 总被引:9,自引:8,他引:1
One non-saturable and two saturable transport systems were demonstrated for taurine in rat brain slices. One of the saturable systems, designated β, is characterized by a high asnity for taurine and a low transport capacity, while the other, designated ω, by a low affinity and a high transport capacity. 2,4-Dinitrophenol inhibited the saturable transport of taurine non-competitively, while hypotaurine. β-alanine, γ-aminobutyric acid, N-methyl-taurine and L-cysteic acid inhibited transport competitively. It is thus inferred that the hypothetical carrier sites of taurine at cell membrane recognize to an equal degree strongly ionized electropositive and electronegative ends of an acceptable molecule separated by two or three carbon atoms. Two is the minimal and also the optimal carbon chain length in an acceptable molecule. 相似文献