Chemical composition of the lipopolysaccharides of Ectothiorhodospira shaposhnikovii,Ectothiorhodospira mobilis,and Ectothiorhodospira halophila

Lipopolysaccharides were isolated from the moderate halophilic Ectothiorhodospira shaposhnikovii slight to and Ectothiorhodospira mobilis and from the extremely halophilic Ectothiorhodospira halophila by the hot phenol-water and purified by the phenol-chloroform-petroleum ether methods. The isolated lipopolysaccharides of all three species contained 3-deoxy-d-manno-octulosonic acid and d-glycero-d-mannoheptose indicating the existence of a core. They contained additionally glucose and uronic acids (E. shaposhnikovii and E. mobilis) or glucose, uronic acids and threonine (E. halophila). Sodium deoxycholate gel-electrophoresis of the three lipopolysaccharides, each showing only one major band, indicated R-type character of the lipopolysaccharides of the three Ectothiorhodospira species.The lipid A fractions of the lipopolysaccharides from E. shaposhnikovii and E. mobilis represented phosphorylated mixed lipid A types with both 2,3-diamino-2,3-dideoxy-d-glucose and d-glucosamine. The lipid A from E. halophila contained also phosphate and 2,3-diamino-2,3-dideoxy-d-glucose but only traces of d-glucosamine, which would indicated lipid A_DAG. The fatty acid spectra were characterized by amide-bound 3-OH-10:0 and 3-OH-12:0 (E. shaposhnikovii), 3-OH-10:0 (E. mobilis), or 3-OH-10:0,3-OH-14:0, and 3-oxo-14-0 (E. halophila). The predominant ester-bound fatty acids were 14:0 and 16:0 (E. shaposhnikovii and E. mobilis), or 12:0 and 14:1 (E. halophila).Abbreviations DAG 2,3-diamino-2,3-dideoxy-d-glucose - Kdo 3-deoxy-d-manno-octulosonic acid - GlcA glucuronic acid - GalA galacturonic acid - GC-MS combined gas liquid chromatographymass spectrometry - GlcN Glucosamine - DOC sodium deoxycholate - LPS lipopolysaccharide - PAGE polyacrylamide gel electrophoresis - PCP phenol-chloroform-petroleum ether     

假尾孢属一新种

报道生于蓼属Polygonum sp.植物上的假尾孢属1个新种:余氏假尾孢Pseudocercospora yui Y.L.Guo.文中提供了新种的拉丁文简介、形态描述、绘图及讨论.模式标本保存在中国科学院微生物研究所菌物标本馆(HMAS).     

球束梗孢属一新种

描述了采自湖北省神农架的球束梗孢属一新种,神农架球束梗孢Gibellula shennongjiaensis,该菌分生孢子梗表面粗糙,瓶梗为棒状,5.4–10.8×1.1–2.2μm。分生孢子为圆柱形或纺锤形,透明,表面光滑,3.2–6.5×1.1–1.6μm。具糙梗孢式共无性型的产孢结构,其分生孢子梗由不规则的菌丝组成,产孢细胞不规则形状,分生孢子无色,光滑,线形,12.1–15.2×1.4–2.1μm。模式标本保存在贵州大学菌种保藏中心(GZAC-SNJ2012,主模式)。     

The primary structure of the antenna polypeptides of Ectothiorhodospira halochloris and Ectothiorhodospira halophila. Four core-type antenna polypeptides in E. halochloris and E. halophila.

Antenna polypeptides from two species of the family Ectothiorhodospiraceae have been investigated. By means of gel filtration and subsequent high-performance liquid chromatography, at least five polypeptides were isolated from each of Ectothiorhodospira halochloris and Ectothiorhodospira halophila. The majority of their primary structures was identified by Edman degradation. Comparison of these polypeptide sequences with the known primary structures of antenna polypeptides from various purple non-sulfur bacteria revealed interesting new aspects with regard to the structure of the core-peripheral antenna system. E. halochloris and E. halophila contain two pairs of alpha- and beta-polypeptides each with typical primary structure elements of core complexes, indicating a modified antenna complex organization.     

寄螨属1新种和革索螨属1新种(蜱螨亚纲:中气门目:寄螨科)

记述寄螨属1新种:简单寄螨Parasitus simplendogynii sp.nov.和革索螨属1新种:泾源革索螨Gamasodes jingyuanensis sp.nov.     

Carotenoid composition in the genus Ectothiorhodospira pelsh

Summary The three species of the genus Ectothiorhodospira have been found to contain the carotenoids of the normal spirilloxanthin series, spirilloxanthin itself being the major component. While the carotenoid composition of E. mobilis and E. shaposhnikovii is not sufficiently different to allow interspecific differentiation, that of E. halophila differs from the two former species by its lack of rhodopin, and its higher amount of spirilloxanthin.     

The porins from the halophilic species Ectothiorhodospira shaposhnikovii and Ectothiorhodospira vacuolata

Major outer membrane proteins with porin activity were isolated from cell envelopes of the halophilic strains Ectothiorhodospira shaposhnikovii N1 and Ectothiorhodospira vacuolataβ1. The porins were obtained as oligomers. They dissociated into monomers by heat or EDTA treatment. The molecular masses of the monomers were determined by mass spectrometry to be 39,285 and 37,160 Da for E. shaposhnikovii N1 and E. vacuolataβ1, respectively. Both were shown by analytical ultracentrifugation to be trimers of about 112,000 Da. Circular dichroism spectra indicated predominantly β-sheet structure. The 18 N-terminal amino acid sequences of the two porins were identical except for the amino acids in positions 12 and 14. No sequence similarity with the primary structure of known porins was found. In reconstitution experiments with lipid bilayers, the porins of E. shaposhnikovii N1 and E. vacuolataβ1 formed channels with a single-channel conductance of 1.5 and 0.7 nS, respectively, in 1 M KCl. The single-channel conductance saturated with increasing salt concentration, indicating a putative binding-site for anions in the channel since both porins exhibited anion-selectivity. For the porin of E. vacuolataβ1, but not for that of E. shaposhnikovii N1, an influence of detergent concentration on the single-channel conductance was observed. Received: 3 April 1996 / Accepted: 31 May 1996     

A new species of the genus Trematosphaeria from China

A new species of the genus Trematosphaeria was isolated and identified from the soil in Jiuzhaigou Nature Reserve, southwestern China. The new taxon was studied based on morphological characters and phylogenetic analyses of three nuclear loci, i.e., the internal transcribed spacer rDNA operon (ITS), and large and small subunit nuclear ribosomal DNA (nuc28S rDNA, nuc18S rDNA). The analyzed Trematosphaeria species were clustered in several clades within the Pleosporales and the genus was shown to be polyphyletic. The new species is introduced as Trematosphaeria terricola, and it is characterized by short-papillate ascomata with suborbicular ostiolum, 3-septate, cinnamon-brown, fusiform ascospores with narrowly rounded ends, verruculose at maturity. Trematosphaeria terricola is compared with morphologically similar species T. confusa, T. mangrovis, and T. pertusa, which differ from the former species in the number of septa, and the shape and size of the ascospores with or without a sheath. This is the first report of a Trematosphaeria isolate from alpine soil in China.     

Chrysocapsopsis rupicola: A new genus and species in the Chrysophyceae

Chrysocapsopsis rupicola gen. et sp. nov. (Chrysophyceae) is described. It differs from Chrysocapsa and the closely related genera Chrysocapselia and Tetrachrysis by possessing both epiphytic and planktonic forms within the same life-history. Reproduction is by budding which produces short irregular branches within a gelatinous mass. Four or more aplanospores are produced from each mother cell; no motile cells were observed.     

Hydrogen-1 nuclear magnetic resonance investigation of high-potential iron-sulfur proteins from Ectothiorhodospira halophila and Ectothiorhodospira vacuolata: a comparative study of hyperfine-shifted resonances

Proton NMR spectra of the oxidized and reduced forms of high-potential iron-sulfur proteins (HiPIPs) were recorded at 200 MHz. The proteins studied were the HiPIPs I and II from Ectothiorhodospira halophila and Ectothiorhodospira vacuolata. Hyperfine-shifted peaks in spectra of the oxidized proteins were assigned to some of the protons of the cysteinyl ligands and aromatic residues at the active site on the basis of their chemical shifts, longitudinal relaxation times, and temperature-dependent behavior. The cysteinyl C beta-H protons were found to resonate downfield (about 100 ppm) and the C alpha-H protons upfield (about-25 ppm). This hyperfine shift pattern is consistent with the observed isotropic shift being contact in origin; it probably results from a pi-spin-transfer mechanism. The large magnitudes of the chemical shifts of peaks assigned to aromatic residues suggest that these residues interact with the iron-sulfur cluster via pi-pi overlap. Some of the hyperfine-shifted peaks observed in water were found to disappear in 2H2O solution. Such resonances probably arise from exchange-labile hydrogens of amino acid residues directly hydrogen bonded to the iron-sulfur cluster. In the case of HiPIPs I and II from E. vacuolata, whose spectra are similar except for the number of such peaks, the relative number of hydrogen bonds inferred to be present in the oxidized and reduced proteins qualitatively explains the difference between their midpoint redox potentials. On the other hand, for E. halophila HiPIPs I and II, consideration of the inferred number of hydrogen bonds alone fails to predict the sign of the difference between their midpoint redox potentials.(ABSTRACT TRUNCATED AT 250 WORDS)     

Crystallization of a high potential iron-sulfur protein from the halophilic phototrophic bacterium Ectothiorhodospira halophila

Crystals of the high-potential iron-sulfur protein from Ectothiorhodospira halophila strain BN 9626 have been grown from 3.4 to 3.5 M ammonium sulfate solutions at pH 7.5. The crystals belong to the space group P21 with unit cell dimensions of a = 60.00 A, b = 31.94 A, c = 40.27 A, and beta = 100.5 degrees. There are 2 molecules/asymmetric unit. The crystals diffract to at least 1.8 A, are stable in the x-ray beam, and are suitable for a high resolution x-ray crystallographic analysis.     

A complete relaxation matrix refinement of the solution structure of a paramagnetic metalloprotein: Reduced HiPIP I from Ectothiorhodospira halophila

We have accounted for the effect of paramagnetism on the intensities of NOEs in a 73-residue paramagnetic metalloprotein, the reduced high-potential iron sulfur protein ISO I from Ectothiorhodospira halophila, whose solution structure had been recently solved by us. The paramagnetic effects were dealt with through a suitably modified complete relaxation matrix approach. We have then recalculated the structure through a distance geometry program by minimizing the difference between the sixth roots of the calculated and experimental NOEs. The average RMSD, calculated on residues 4–71, within the structures constituting the two families decreased from 0.67 to 0.46 Å for backbone atoms and from 1.23 to 1.06 Å for all heavy atoms. The structures in the new family are for the most part within the indetermination of the previous, less resolved, family. A few specific differences are detected and related to the presence of non-negligible paramagnetic effects, which are now properly evaluated.     

我国苏铁属一新种

我国苏铁属(苏铁种)一新种, 即念珠苏铁。     

华山松上散斑壳属一新种

根据子囊果的形态及埋生位置和松针上线纹的特征,鉴定出四川二郎山华山松Pinus armandii上散斑壳属一新种,即二郎山散斑壳Lophodermium erlangshanense。对该新种进行了形态学特征的拉丁文和中文描述,并附有形态结构图。模式标本存于四川农业大学林木真菌标本室(FMSAU)。     

中国双缘衣属(双缘衣科)地衣一新种

现分类系统中,双缘衣属隶属于子囊菌门,茶渍纲,厚顶盘亚纲,文字衣目,双缘衣科。全世界有48种,被确认为单系类群的小属。利用形态学、解剖学、生物化学以及分子生物学方法,对采自天山西部的双缘衣属地衣标本进行分类学研究,发现了双缘衣属地衣1个新种：绿盘双缘衣Diploschistes viridis (属于双缘衣亚属)。基于最大似然分析(ML)和贝叶斯分析(BI)构建了基于ITS位点系统发育树,详细描述该种的形态特征、分布及栖息地等情况,并提供了地衣体的彩色图片。