共查询到20条相似文献,搜索用时 15 毫秒
1.
A. Kosiha C. Parthiban Samuele Ciattini Laura Chelazzi 《Journal of biomolecular structure & dynamics》2013,31(16):4170-4181
Protein binding, DNA binding/cleavage and in vitro cytotoxicity studies of 2-((3-(dimethylamino)propyl)amino)naphthalene-1,4-dione (L) and its four coordinated M(II) complexes [M(II) = Co(II), Cu(II), Ni(II) and Zn(II)] have been investigated using various spectral techniques. The structure of the ligand was confirmed by spectral and single crystal XRD studies. The geometry of the complexes has been established using analytical and spectral investigations. These complexes show good binding tendency to bovine serum albumin (BSA) exhibiting high binding constant values (105 M?1) when compared to free ligand. Fluorescence titration studies reveal that these compounds bind strongly with CT-DNA through intercalative mode (Kapp 105 M?1) and follow the order: Cu(II) > Zn(II) > Ni(II) > Co(II) > L. Molecular docking study substantiate the strength and mode of binding of these compounds with DNA. All the complexes efficiently cleaved pUC18-DNA via hydroxyl radical mechanism and the Cu(II) complex degraded the DNA completely by converting supercoiled form to linear form. The complexes demonstrate a comparable in vitro cytotoxic activity against two human cancer cell lines (MCF-7 and A-549), which is comparable with that of cisplatin. AO/EB and DAPI staining studies suggest apoptotic mode of cell death, in these cancer cells, with the compounds under investigation. 相似文献
2.
《Journal of liposome research》2013,23(4):813-827
AbstractTwo strategies for increasing liposome stability in vivo are described in this review. The first strategy involves the encapsulation of liposomes within polymeric microcapsules of alginate-poly(L-lysine) that retained the liposomes inside but allowed the outward diffusion of proteins of 100 kDa or less, once they were released from the encapsulated liposomes. In vivo studies revealed that the microencapsulated liposome systems (MELs) extended the delivery of a model antigen, bovine serum albumin (BSA), for more that 80 days, resulting in the prolonged production of high levels of antigen-specific antibodies. The antibody levels were higher that those obtained with rats injected with BSA in complete Freund's adjuvant, or in liposomes. The unique construction of MELs enabled also the enzymatically-triggered pulsatile delivery of proteins from encapsulated liposomes, which was not possible before with liposomes. 相似文献
3.
Reinvestigation of alloantisera containing antibodies to murine antigen H-2.7 revealed that the crucial recombinant, A.TFR1 (H-2
an1
), which was reported to separate theH-2G locus from theSs-Slp loci, has ak-like instead off-like H-2.7 antigen. Therefore, the crossover position inH-2
an1
and the position of the G locus in theH-2 map are now uncertain. By using the hemagglutination-serum inhibition test, anti-H-2.7 reactive substance was found to be present in normal mouse serum in a strain-specific manner. Tissue distribution study by absorption analysis indicated that H-2.7 antigen is present, in addition to RBCs, on spleen and lymph node cells, but is absent on thymus cells. Thirty B10.W congenic lines were analysed for the presence of the H-2.7 antigen. Two lines (B 10.CHA2 and B 10.KPA44) were found to be H-2.7 positive by both direct hemagglutination and absorption tests.Abbreviations used in this paper ACT
Ammonium chloride Tris-buffer
- BSA
Bovine serum albumin
- HA
Hemagglutination
- HASI
Hemagglutination serum inhibition
- HBSS
Hanks balanced salt solution
- PBS
Phosphate buffered saline
- PVP
Polyvinylpyrrolidone
- RBCs
Red blood cells 相似文献
4.
《International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology》1991,18(5):499-502
To elucidate a factor required for tumor-imaging 99mTc-labeled radiopharmaceuticals, in vivo behaviors of 99mTc-l-cysteine (99mTc-Cys) and 99mTc-2-mercaptoethylamine (99mTc-ME) were compared with that of 99mTc-dl-homocysteine (99mTc-Hcy) which had been found to accumulate in several experimental tumors. When these three complexes were intravenously injected into mice bearing Ehrlich solid tumor, their tumor affinity was found to depend on their binding ability to serum albumin; 99mTc-Hcy, the albumin-binding ability of which was highest of the three, was the most tumor-tropic. When the albumin-bound complexes of these three were injected, their tumor distributions were enlarged. These results suggest the importance of serum albumin in serving as a carrier for the transport of 99mTc-Hcy-related compounds to tumor tissue. 相似文献
5.
Elena Krayukhina Masanori Noda Kentaro Ishii Takahiro Maruno Hirotsugu Wakabayashi Minoru Tada 《MABS-AUSTIN》2017,9(4):664-679
A number of studies have attempted to elucidate the binding mechanism between tumor necrosis factor (TNF) and clinically relevant antagonists. None of these studies, however, have been conducted as close as possible to physiologic conditions, and so the relationship between the size distribution of TNF-antagonist complexes and the antagonists' biological activity or adverse effects remains elusive. Here, we characterized the binding stoichiometry and sizes of soluble TNF-antagonist complexes for adalimumab, infliximab, and etanercept that were formed in human serum and in phosphate-buffered saline (PBS). Fluorescence-detected sedimentation velocity analytical ultracentrifugation analyses revealed that adalimumab and infliximab formed a range of complexes with TNF, with the major complexes consisting of 3 molcules of the respective antagonist and one or 2 molcules of TNF. Considerably greater amounts of high-molecular-weight complexes were detected for infliximab in human serum. The emergence of peaks with higher sedimentation coefficients than the adalimumab monomer as a function of added human serum albumin (HSA) concentration in PBS suggested weak reversible interactions between HSA and immunoglobulins. Etanerept exclusively formed 1:1 complexes with TNF in PBS, and a small amount of complexes with higher stoichiometry was detected in human serum. Consistent with these biophysical characterizations, a reporter assay showed that adalimumab and infliximab, but not etanercept, exerted FcγRIIa- and FcγRIIIa-mediated cell signaling in the presence of TNF and that infliximab exhibited higher potency than adalimumab. This study shows that assessing distribution profiles in serum will contribute to a more comprehensive understanding of the in vivo behavior of therapeutic proteins. 相似文献
6.
Yun Li Nana Wang Dong Lin Xiaohui Liu Yong Yang 《Journal of biomolecular structure & dynamics》2020,38(17):4977-4996
AbstractTwo new nickel (II) triphenylphosphine complexes derived from tridentate aroylhydrazone ligands [H2L1 = 2-hydroxy-3-methoxybenzylidene)benzohydrazone and H2L2 = N′-(2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzoylhydrazone] and triphenylphosphine were prepared and their molecular structures were determined by single crystal X-ray diffraction analysis. Both nickel(II) complexes showed slightly distorted square planar geometry with one tridentate aroylhydrazone ligand coordinated through ONO donor atoms and one triphenylphosphine ligand coordinated to the nickel center through the phosphorus atom. DNA interaction studies indicated that both complexes possessed higher affinity to herring sperm DNA (HS-DNA) than the corresponding free aroylhydrazone ligand. Molecular docking investigations showed that both complexes could bind to DNA through intercalation of the phenyl rings between adjacent base pairs in the double helix. Meanwhile, bovine serum albumin (BSA) binding studies revealed the complexes could effectively interact with BSA and change the secondary structure of BSA. Further pharmacological evaluations of the synthesized complexes by in vitro antioxidant assays demonstrated high antioxidant activity against NO· and O2˙? radicals. The anticancer activity of each complex was assessed through in vitro cytotoxicity assays (CCK-8 kit) toward A549 and MCF-7 cancer cell and normal L-02 cell lines. Significantly, the Ni(II) complex derived from H2L1 ligand was found to be more effective cytotoxic toward MCF-7cancerous cell with the IC50 value equaled 9.7?μM, which showed potent cytotoxic activity over standard drug cisplatin. Abbreviations A549 human lung carcinoma cell BSA bovine serum albumin CCK-8 Cell Counting Kit-8 DFT density functional theory DNA deoxyribonucleic acid DPPH˙ 2,2-diphenyl-1-picrylhydrazyl H2L1 2-hydroxy-3-methoxybenzylidene)benzohydrazone N′-(2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzoylhydrazone H2L2 N′-(2-hydroxy-3-methoxybenzylidene)-2-hydroxybenzoylhydrazone HOMO highest occupied molecular orbital IC50 the 50% activity L-02 human normal liver cell LOMO lowest unoccupied molecular orbital (LUMO) MCF-7 human breast carcinoma cell NO˙ nitric oxide O2˙? superoxide anion SOD superoxide dismutase Communicated by Ramaswamy H. Sarma 相似文献
7.
The influence of Igh-1 genes on the class and subclass distribution of oxazolone-specific antibodies
Previous studies have demonstrated that the level of the oxazolone-specific antibody response induced by contact sensitization is under the control of H-2 and Igh-1-linked genes. The aim of the present study was to clarify the role of H-2 and Igh-1 genes in the regulation of antibody affinity and isotype composition of oxazolone-specific antibodies. Analysis of the antibody response to oxazolone has revealed different ratios of IgG2a and IgG2b antibodies in mice carrying the Igh-1
b allele and in strains carrying alleles a, c, and e. The characteristic ratio of IgG2a and IgG2b isotypes persisted during the whole period of the primary and secondary antibody response of CBA and CBA-Ig
b
Igh-C congenic mice. The Igh-1-linked genes influenced the isotype distribution and not the affinity of oxazolone-specific antibodies induced by contact sensitization.Abbreviations used in this paper c.Ig
chicken immunoglobulin
-
Igh-C
constant region of immunoglobulin heavy chain
- DNCB
2,4-dinitrochlorobenzene
- DTH
delayed-type hypersensitivity
- FITC
fluorescein isothiocyanate
- Igh-1
cluster of structural heavy chain allotype genes of IgG2a
- KLH
keyhole limpet hemocyanin
- KSCN
potassium-sulfocyanid
- MHC
major histocompatibility complex
- Ox
oxazolone (4-ethoxymethylene-2-phenyl-5-oxazolone
- Ox-BSA
oxazolone-bovine serum albumin
- Ox-cap
oxazolone-capronic acid
- Ox-MSA
oxazolone-mouse serum albumin
- NP
4-hydroxy-3nitrophenyl acetyl
- PVP
polivinylpirrolidon
- RIA
radioimmunoassay
- SRBC
sheep red blood cell
- TH
T helper
- TS
T suppressor
-
Igh-V
variable region of immunoglobulin heavy chain 相似文献
8.
Neuraminidases (sialidases) catalyse the removal of terminal sialic acid from glycoconjugates. Bacterial pathogens often utilize neuraminidases to scavenge host sialic acid, which can be utilized either as a nutrient or as a decorating molecule to disguise themselves from host immune attacks. Herein, a putative neuraminidase (TDE0471) was identified in Treponema denticola, an oral spirochaete associated with human periodontitis. TDE0471 is a cell surface‐exposed exo‐neuraminidase that removes sialic acid from human serum proteins; it is required for T. denticola to grow in a medium that mimics gingival crevice fluid, suggesting that the spirochaete may use sialic acid as a nutrient in vivo. TDE0471 protects T. denticola from serum killing by preventing the deposition of membrane attack complexes on the bacterial cell surface. Animal studies revealed that a TDE0471‐deficient mutant is less virulent than its parental wild‐type strain in BALB/C mice. However, it causes a level of tissue damage similar to the wild type in complement‐deficient B6.129S4‐C3tm1Crr/J mice albeit the damage caused by both bacterial strains is more severe in these transgenic mice. Based on these results, we propose that T. denticola has evolved a strategy to scavenge host sialic acid using its neuraminidase, which allows the spirochaete to acquire nutrients and evade complement killing. 相似文献
9.
Yukio Ikehara Kimimitsu Oda Keitaro Kato 《Biochemical and biophysical research communications》1976,72(1):319-326
The conversion site of proalbumin into serum albumin was investigated in the subcellular fractions of rat liver labeled with [3H] leucine in vivo. In the cisternae-rich fraction of the Golgi complex as well as in the microsomal fraction most of the labeled albumin was detected as proalbumin, while in the secretory vesicles, which were obtained in increased amount by oral administration of ethanol, more than 70% of the labeled albumin was found as serum type, indicating that conversion of proalbumin into serum albumin occurs within the secretory vesicles in rat liver. Little accumulation of albumin was observed in colchicine-treated rats. 相似文献
10.
We have analyzed the genetic control of susceptibility to suppression by 1-J+, suppressor-T-cell derived factors (TsF) specific for the synthetic polymer L-glutamic acid50-L-tyrosine50 (GT). GT-TsF activity was measured as specific inhibition of proliferative responses to GT developed in cultures of lymph-node T cells from mice primed with GT complexed to methylated bovine serum albumin (GT-MBSA). These experiments demonstrated that there is no MHC-encoded genetic restriction between donors and recipients of GT-TsF in suppression of proliferative responses. We have also confirmed the observations that mice of the H-2
b, H-2
d, and H-2
khaplotypes can produce GT-TsF, whereas H-2
amice do not, and that H-2
b, H-2
d, and H-2
kmice are sensitive to GT-TsF from all producer strains, whereas H-2
bmice are not sensitive to GT-TsF from any strain. Analysis of the effect of GT-TsF on responses by mice bearing recombinant haplotypes suggests that at least two genes are required for susceptibility to GT-TsF and that these genes show coupled complementation.Abbreviations used in this paper GAT
random linear terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10
- GAT-MBSA
GAT complexed to methylated bovine serum albumin
- GATTsF
GAT-specific-T-cell derived suppressor factor
- GT
random linear copolymer of L-glutamic acid50-L-tyrosine50
- GT-MBSA
GT complexed methylated bovine serum albumin
- GT-TsF
GT, specific, T-cell derived suppressor factor
-
3H-TdR
tritiated thymidine
- Ir gene
immune response gene
- MBSA
methylated bovine serum albumin
- MEM
minimal essential media
- MHC
major histocompatibility complex
- PFC
plaque-forming cell(s)
- PPD
purified protein derivative of M. tuberculosis H37Ra 相似文献
11.
Fatemeh Mohammadi 《Journal of biomolecular structure & dynamics》2020,38(10):3059-3073
AbstractThe side effects and resistance of metal-based anticancer drugs prompted us to synthesis a novel series of five Pd(II) complexes of the type [Pd(8-QO)(AA)]; where 8-QO?=?anion of 8-hydroxyquinoline and AA?=?anions of amino acids having nonpolar aliphatic side chain such as glycine (–H), alanine (–CH3), valine (–CH(CH3)2), leucine (–CH2–CH(CH3)2) and isoleucine (–CH(CH3)CH2–CH3). The complexes have been characterized with the help of FT-IR, UV–Vis, one and two-dimensional 1H-NMR, elemental analysis and conductivity measurements. On the basis of these characterization data, a four coordinated square planar geometry for all of these complexes have been proposed. The compounds were screened for their in vitro activities against human cancer cell line, MOLT-4 and their 50% inhibition concentration were ascertained by means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Since four out of the five newly synthesized compounds were found to be more active than the standard anticancer drug, cisplatin, their detailed interaction with calf thymus DNA (as a target) and bovine serum albumin (BSA) (as a carrier) were also carried out by utilizing absorption spectra, fluorescence spectra and ethidium bromide displacement studies. In these experiments, several binding and thermodynamic parameters were also calculated. These results suggested that hydrogen binding and van der Waals forces play a major role in the interaction between metal complexes with CT-DNA and BSA.Communicated by Ramaswamy H. Sarma 相似文献
12.
Using four different protein antigens, two different strains of mice, and various immunization protocols, we have studied production in mice of immunological enhancement antibodies that specifically suppress induction of delayed hypersensitivity. Primary assay of these antibodies was in vivo, because no in vitro test used detected them dependably. Any antigen priming that favored initiation of humoral antibody responses prepared mice to make these contrasensitizing antibodies vigorously following appropriate boosting. The method of boosting usually was more important than that of priming, high titers regularly developing only when primed mice were boosted with much antigen in a short time and were bled a few days later. The presence or absence of delayed hypersensitivity was immaterial. CAF1 mice made these antibodies better than CF-1 mice, and antigen effectiveness correlated with propensity to induce humoral antibody formation in mice, decreasing from ovalbumin through human serum albumin and bovine serum albumin to methylated human serum albumin. In certain antigenmouse combinations (e.g., ovalbumin in CAF1 mice) immunosuppressive antibody production was vigorous and prolonged; in others (e.g., bovine serum albumin in CF-1 mice) it was moderate and brief. From our results one can predict what conditions should induce formation of strongly enhancing/contrasensitizing antisera, and speculate that these conditions also should elicit strong, active immunologic tolerance for averting induction of delayed hypersensitivity. 相似文献
13.
14.
《MABS-AUSTIN》2013,5(2):344-351
Serum albumin is the major determinant of blood colloidal osmotic pressure acting as a depot and distributor of compounds including drugs. In humans, serum albumin exhibits an unusually long half-life mainly due to protection from catabolism by neonatal Fc receptor (FcRn)-mediated recycling. These properties make albumin an attractive courier of therapeutically-active compounds. However, pharmaceutical research and development of albumin-based therapeutics has been hampered by the lack of appropriate preclinical animal models. To overcome this, we developed and describe the first mouse with a genetic deficiency in albumin and its incorporation into an existing humanized FcRn mouse model, B6.Cg-Fcgrttm1Dcr Tg(FCGRT)32Dcr/DcrJ (Tg32). Albumin-deficient strains (Alb-/-) were created by TALEN-mediated disruption of the albumin (Alb) gene directly in fertilized oocytes derived from Tg32 mice and its non-transgenic background control, C57BL/6J (B6). The resulting Alb-/- strains are analbuminemic but healthy. Intravenous administration of human albumin to Tg32-Alb-/- mFcRn-/- hFcRnTg/Tg) mice results in a remarkably extended human albumin serum half-life of ~24 days, comparable to that found in humans, and in contrast to half-lives of 2.6–5.8 d observed in B6, B6-Alb-/- and Tg32 strains. This striking increase can be explained by the absence of competing endogenous mouse albumin and the presence of an active human FcRn. These novel albumin-deficient models provide unique tools for investigating the biology and pathobiology of serum albumin and are a more appropriate rodent surrogates for evaluating human serum albumin pharmacokinetics and albumin-based compounds. 相似文献
15.
Akiyuki Yokoyama Akiyuki Yokoyama Hiroyuki Sakakibara Akiyuki Yokoyama Hiroyuki Sakakibara Alan Crozier 《Free radical research》2013,47(10):913-921
Quercetin has strong antioxidant potency. Quercetin-3′-O-sulphate (Q3′S) and quercetin-3-O-glucuronide (Q3GA) are the main circulating metabolites after consumption of quercetin-O-glucoside-rich diets by humans. However, information about how these quercetin metabolites function in vivo is limited. Hence, this study evaluated the efficacy of Q3′S and Q3GA for the protection of oxidative injury using in vitro and in vivo experiments. Peroxynitrite-mediated hepatic injury in rats was induced by administration of galactosamine/lipopolysaccharide (GalN/LPS). Twenty-four hours after GalN/LPS treatment, plasma ALT and AST levels δ increased significantly. However, pretreatment with 4G-α-D-glucopyranosyl rutin, a quercetin glycoside (30 mg/kg body weight), prevented these increases and reduced nitrotyrosine formation, indicating that consumption of quercetin glycosides prevent oxidative hepatotoxicity. Moreover, physiological levels of Q3′S and Q3GA (1 µM) effectively prevented peroxynitrite-induced nitrotyrosine formation in human serum albumin in in vitro experiments. These findings indicate peroxynitrite-induced oxidative hepatotoxicity is protected by the in vivo metabolites of quercetin, Q3′S and Q3GA. 相似文献
16.
Phagocytic Cells in the Peripheral Blood of the Dogfish Scyliorhinus canicula L. II. In Vivo Studies
In vivo phagocytosis by peripheral blood leucocytes of the dogfish Scyliorhinus canicula L. was examined by monitoring the fate of a variety of injected materials, both particulate and soluble, in normal and immunised fish. Carbon, yeasts and bacteria were phagocytosed by monocytes, thrombocytes and type 1 granulocytes (neutrophils). Quantitative in vivo antigen clearance studies employed five species of bacteria, yeast and KLH. After an initial significant decrease of these antigens in the circulation, low numbers of viable bacteria and yeasts and low concentrations of KLH persisted for long periods after injection. Previous exposure to several of these antigens had little or no effect. 相似文献
17.
Kunihiko Maeda Marie H. Kosco-Vilbois Greg F. Burton Andras K. Szakal John G. Tew 《Cell and tissue research》1995,279(1):47-54
Intercellular adhesion molecule-1 (ICAM-1)1 has been implicated in the development of germinal center reactions in vitro, and the present study was undertaken to determine the distribution of ICAM-1 in active germinal centers in vivo and in murine secondary lymphoid tissues in general. Anti-ICAM-1-specific monoclonal antibodies were used in conjunction with immunohistochemistry at both the light and ultrastructural levels of resolution. Examination of cryostat sections of lymph nodes, spleens, and Peyer's patches revealed that anti-ICAM-1 distinctly labeled cells in the light zones of germinal centers, a few cells in the T cell zones (e.g. paracortex of lymph nodes), cells in the sinus floor of the subcapsular sinuses of lymph nodes, and high endothelial venules (HEV). Ultrastructural studies revealed that the cells labeling with anti-ICAM-1 in germinal centers were follicular dendritic cells (FDC) which appeared to have more ICAM-1 than any other cell type. The surfaces of well-developed, intricate, convoluted FDC processes were intensely labeled even under conditions where B cells appeared negative. Interdigitating cells (IDC) were also labeled as were certain endothelial cells in the HEV. The cells in the subcapsular sinus floor labeling with anti-ICAM-1 were the antigen transporting cells (ATC) that carry antigen-antibody complexes into lymph node follicles. We suspect ATC are FDC precursors which mature into FDC in the follicles. Interestingly, FDC, IDC, and ATC are 3 important accessory cells known to handle antigens in specific compartments of lymphoid tissues. The marked localization of this adhesion molecule on these critical antigen handling cells supports the concept that ICAM-1 is important in providing the intercellular adhesion necessary for optimal initiation of immune responses in vivo.Abbreviations
ICAM-1
Intercellular adhesion molecule-1
-
LFA-1
leukocyte functional antigen-1
-
IDC
interdigitating cells
-
ATC
antigen transporting cells
-
FDC
follicular dendritic cells
-
HEV
high endothelial venules
-
DC
dendritic cells
-
PBS
phosphate-buffered saline
-
PLP
periodate-lysine-4% paraformaldehyde
-
GPLP
periodate-lysine-0.1% glutaraldehyde-2% paraformaldehyde
-
EM
electron microscopy
-
HRP
horseradish peroxidase
-
DAB
diaminobenzidine tetrahydrochloride
-
HSA
human serum albumin 相似文献
18.
19.
Immune responsiveness to Ambrosia artemishfolia (short ragweed) pollen allergen Amb a VI (Ra6) is associated with HLA-DR5 in allergic humans 总被引:1,自引:0,他引:1
David G. Marsh Linda R. Freidhoff Eva Ehrlich-Kautzky Wilma B. Bias Marianne Roebber 《Immunogenetics》1987,26(4-5):230-236
The relationship between HLA type and specific immune responsiveness toward ultrapure Ambrosia artemisiifolia (short ragweed) pollen allergen Amb a VI (Ra6) was explored in a genetic-epidemiologic study of groups of 116 and 81 Caucasoid subjects who were skin-test \ positive (ST–) toward common environmental allergens. Specific immune responsiveness to Amb a VI was assessed by measuring serum IgE and IgG antibodies (Abs) by double Ab radioimmunoassay in both ST– groups. Significant associations were found between IgE Ab responsiveness to Amb a VI and the possession of HLA-DR5; P values for the two groups were, respectively, 7 × 10–7 and 1 × 10–3 by nonparametric analyses, and 4 × 10–11 and 5 × 10–8 by parametric analyses. The levels of significance for the associations between HLA-DR5 and IgG Ab responsiveness were highly dependent on the extent of ragweed immunotherapy (Rx) within the patient group; by parametric statistics, the associations were 10–11 for the group that had received relatively little Rx and 2 × 10–3 for the group that had received more intensive Rx. These results provide further striking evidence for the existence of specific HLA-linked human Ir genes involved in responsiveness toward inhaled allergens and illustrate the usefulness of the allergy model in studies of the genetic basis of human immune responsiveness. Extension of these studies to investigation of structure-function relationships involved in antigen recognition by Ia molecules and the T-cell receptor will lead to a better understanding of human susceptibility toward immunologic diseases.Abbreviations used in this paper Ab
antibody
-
Amb a VI
Amb a V, new IUIS nomenclature for Ambrosia artemisiifolia pollen allergens nos. 6 and 5 (short ragweed Ra6 and Ra5) (Marsh et al. 1986b)
-
Lol p II, III
new IUIS nomenclature for Lolium perenne pollen allergens II and III (perennial rye grass, Rye II and Rye III) (Marsh et al. 1986b)
- BBS
borate-buffered physiologic saline
- BSA
bovine serum albumin
- DARIA
double-antibody radioimunoassay
- Ia
immune-associated
- PAGE
polyacrylamide gel electrophoresis
- RIST
radioimmunosorbent test
- Rx
immunotherapy
- SDS
sodium dodecyl sulfate
- ST
skin test 相似文献
20.
Michel Fischbach Hanwei Cao Miguel Diez Ibanez Christos Tsaconas Sami Alouani Frédéric Montandon Mohammed El Baraka Prudent Padieu Michel Dreano Martine Chessebeuf-Padieu 《Cell biology and toxicology》1991,7(4):327-345
Collagenase isolated rat hepatocytes were transfected with liposome encapsulated pEJ (LE-pEJ), a plasmid carrying the human cellular activated Ha-rasEJ oncogene. A proliferative cell line was cloned from these cells transfected in vitro. It secreted per day 0.87 µg albumin and 0.32 µg transferrin per 106 cells, and 11.06 nmol free and conjugated bile acids (BA) per mg protein. Also, it metabolized 2-acetylaminoflourene (2-AFAF) into N- and ring-hydroxylated metabolites and 2-aminofluorene at rates of 1.50, 9.73, and 1.98 nmol/mg cell protein/24 hr, respectively. Rats were i.v. injected with both LE-pEJ and LE-p17hGHnneo carrying the hGH cDNA gene, and secreted hGH in the plasma which induced the synthesis of anti-hGH antibodies. A cell line was cloned from cultures of primary hepatocytes isolated from the liver of transfected rats. After 2 to 3 months in culture, this cell line secreted per day 18.9 µg albumin and 11.0 µg transferrin per 106 cells, 38.75 nmol total BA per mg cell protein, and up to 31 ng hGHper 106 cells without cloning hGH recombinant cells. A 24 hr control culture of primary hepatocytes isolated from non transfected rats secreted 25.5 µg albumin and 11.7 µg transferrin per 106 cells, and produced 21.64 nmol total BA and 2.13 nmol N-OH-2-AFAF per mg cell protien. Hence, Ha-ras
EJ transfection of either hepatocytes in vitro or liver cells in vivo, initiated cell cycles leading to presumptive proliferating hepatocytes which express liver function.Abbreviations
BWE
basal Williams' medium E
- FBS
fetal bovine serum
-
F10 or F12
basal Ham's F10 or F12 medium
- Ha-ras
EJ
EJ allele of the human cellular ras oncogen of Harvey
-
hGH
human growth hormone
-
hsp
heat shock protein gene
-
LE-p
liposome encapsulated plasmid
- N-OH-2-AFAF
N-hydroxy-2-acetylaminofluorene
- RLECC
rat liver epithelial cell
- SF
serum-free
- SS
serum-supplemented
- UGG
serum substitute UGltroser G®
- 1-OH-, 3-OH-2-AFAFF
1-hydroxy-, 3-hydroxy-2-acetylaminofluorene
- 2-AFAF
2-acetylaminofluorene
- 2-AFF
2-aminofluorene 相似文献