Effect of intraoperative local application of 3% hydrogen peroxide on pocket infections following cardiac implantable electronic device implantation: An observational study

Aims

The aim of this study was to analyze whether local application of 3% hydrogen peroxide (H2O2) additionally to standard antibiotic prophylaxis following implantation of cardiac implantable electronic devices (CIED) reduces the incidence of pocket infections (PI).

Occurrence of Torsade de Pointes prior to complete lead connection during pacemaker implantation

Torsade de Pointes (TdP) can be triggered by a pacing spike on the T-wave, due to pacemaker undersensing. However, it is not widely known that this phenomenon can occur even during pacemaker implantation. An 84-year-old woman underwent pacemaker implantation for the treatment of a complete atrioventricular block with dyspnea. During the procedure, immediately following ventricular lead insertion and before torque wrench tightening, TdP was observed. Ventricular pacing was initiated by inserting the lead into the header of the generator; however, sensing remained unstable. T-waves associated with undersensed PVCs and ventricular pacing occurred simultaneously, resulting in a spike on the T-wave and TdP.     

Bovine peripheral blood MSCs chemotax towards inflammation and embryo implantation stimuli

Mesenchymal stem cells (MSCs) have a great potential in regenerative medicine because of their multipotential and immunoregulatory capacities, while in early pregnancy they could participate in the immunotolerance of the mother towards the embryo. Peripheral blood constitutes an accessible source of MSCs. We successfully isolated peripheral blood MSC (pbMSCs) lines, with or without previous bone marrow mobilization. All pbMSCs lines obtained in both conditions presented classical MSC markers and properties, alkaline phosphatase activity and multipotent capacity to differentiate among adipogenic, osteogenic or chondrogenic lineages, and suppressed the proliferation of T cells. pbMSCs showed migratory capacity without cytokine stimulation while increasing their migration rate in the presence of inflammatory or embryo implantation stimuli. Interestingly, in contrast to MSCs derived from endometrial tissue, three pbMSCs lines also showed increased migration towards the IFN‐τ implantation cytokine. Moreover, the secretome produced by an early implantation stage embryonic trophectoderm cell line showed a chemoattractant effect in pbMSCs. Our results suggest that circulating MSCs are present in the peripheral blood under healthy conditions. The fact that both the inflammation and implantation signals induced pbMSCs chemotaxis highlights MSC heterogeneity and suggests that their migratory capacity may differ according to their tissue of origin and would suggest the possible active recruitment of MSCs from bone marrow during pregnancy to repress the immune response to prevent the embryo rejection by the maternal organism.     

Evaluation of the role of leukotrienes on ovum implantation in mice

Prostaglandins are considered to be one of the important mediators of ovum implantation. Various lipoxygenase products also have been implicated along with PGs for this process. A specific rather than preferential inhibitor of 5-lipoxygenase is used to investigate the role of leukotrienes in the event of implantation and decidualization process in mice. AA861, a selective inhibitor of 5-lipoxygenase is used in different dose levels like 50,100 and 500 μg in (A) intact pregnant mice (on D1-D4 and D2-D4 and D4); (B) delayed mice on (D3-D8); (C) pseudopregnant traumatized mice (on D1-D4). All the experimental animals of group A were killed on D6. Estrogen injected delayed animals of group  were killed 48 h after the induction of implantation. Implantation sites were counted as blue spot and compared with those of control animals. Traumatized animals of group C were killed 24 h after the mechanical traumatization and uterine weights were compared with those of vehicle treated controls. Results show that AA861 could not inhibit ovum implantation in either intact or ovariectomized delayed animals. It also did not show any adverse effect on tubal transport or development of embryos. AA861 did not have any inhibitory role on decidualization of pseudopregnant uteri also. This experiment shows that a selective inhibitor of 5-lipoxygenase enzyme may not impair the implantation in mice indicating a doubt about the involvement of 5-lipoxygenase products in implantation.     

离子注入麦角甾醇酵母选育研究

用10 keV、剂量为2.6×1013N+/cm2～8.0×1014N+/cm2的氮离子注入产麦角甾醇酵母,可产生可遗传的变异.离子注入产麦角甾醇酵母的存活率与注入剂量呈负相关,在存活率为25%～45%,即注入剂量为1.3～2.3×1014N+/cm2时菌种有较高的正变率.最终筛选到的高产菌株YA1和YA2,麦角甾醇得率较出发菌株分别提高了60%和55%.经复筛及传代实验表明高产菌株遗传性能稳定.     

A new method for localizing the landmark of axillary vein and its application

BackgroundAxillary vein puncture is a popular puncture site for pacemaker implantation. However, due to the lacking of body surface markers, the current puncture method is too complicated and affect the popularization and application of axillary vein puncture. Here, we performed a new body surface landmark to make the blind axillary vein puncture simple and easy.MethodsThe study population included 30 patients referred for pacemaker implantation using axillary vein puncture. Digital subtraction angiography (DSA) was used to determine the direction and the surface landmarks of the axillary vein. Medial cusp of thoracic triangle and the coracoid process were directly touched with fingers. The puncture point was about 1 cm below the coracoid, and the needle tip pointed to the medial cusp of thoracic triangle with the angle of 30–60°.ResultsThere was little variation in distribution of axillary vein. The body surface landmark of the junction of the axillary vein and the subclavian vein is on the medial cusp of thoracic triangle. In these 30 patients, blind axillary vein puncture was successful obtained in all patients. There was no pneumothorax and inadvertent arterial puncture. The pacemaker lead wire was placed smoothly. Moreover, the pacemaker pocket was ideally positioned when cut along the puncture point.ConclusionsBlind axillary vein access using the body surface landmark of the thoracic triangle is an effective method for pacemaker implantation and can obvious avoid the complications usually observed with the traditional subclavian vein approach.     

Effects of anandamide on embryo implantation in the mouse

Anandamide (N-arachidonoylethanolamine), an arachidonic acid derivative, is an endogenous ligand for both the brain-type (CB1-R) and spleen-type (CB2-R) cannabinoid receptors. To investigate the possible effects of anandamide on embryo implantation in the mouse, we used a co-culture system in which mouse embryos are cultured with a monolayer of uterine epithelial cells. Our results indicate that 14 nM anandamide significantly promotes the attachment and outgrowth of the blastocysts on the monolayer of uterine epithelial cells, and those effects could be blocked by CB1-R antagonists SR141716A, but not by SR144528, a CB2-R antagonist. It suggests that the effects of anandamide on embryo attachment and outgrowth are mediated by CB1-R. However, 56 nM anandamide is capable of inhibiting the blastocyst attachment and outgrowth, we, therefore, conclude that anandamide may play an essential role at the outset of implantation.     

离子注入微生物诱变育种研究进展

论述了离子注入微生物育种的三大特征 ,介绍了微生物接受注入离子的方法、离子注入机的简单结构和工作情况。概述了近几年来离子注入微生物 ,改良、选育优良工业微生物菌株的应用研究情况 ,分析了今后离子注入微生物育种的发展趋势。     

自蔓延高温合成镍钛形状记忆合金的生物医学基础研究——第二部分 组织内植入实验

目的:研究自蔓延高温合成钛镍形状记忆合金(SHS SMA)生物学组织相容性。材料和方法:将SHS NiTi SMA多孔体制成薄片,植入大白鼠体内,观察埋植材料与组织的结合情况及对组织的影响,周围组织对埋植材料的反应,并用常规锻制的镍钛记忆合金作对照。结果:肉眼观察,SHS NiTi SMA多孔材料与周围组织结合紧密,而NiTi SMA光滑材料与周围组织结合疏松;光镜观察,两和埋植材料在实验初期,周围组织均有轻度的炎症反应;SHS NiTi SMA有孔材料在一月后材料周围纤维组织增生,并有乳头状突起;NiTi SMA光滑材料一月后材料周围呈包膜样改变。电镜观察,有孔材料的孔隙内有组织长入,并随相通的孔隙长入材料的内部。而光滑材料周围呈纤维组织包裹。结论:两种材料均有良好的组织相容性,SHSNiTi SMA的孔隙有利于组织的长入和结合。     

A cross-sectional survey of cadmium biomarkers and cigarette smoking

Cadmium contamination of tobacco may contribute to the health hazards of cigarette smoking. The 2005–2012 United States National Health and Nutrition Examination Survey data provided a unique opportunity to conduct a cross-sectional survey of cadmium biomarkers and cigarette smoking. Among a sample of 6761 participants, we evaluated mean differences and correlations between cadmium biomarkers in the blood and urine and characteristics of never, former and current smokers. We found statistically significant differences in mean cadmium biomarker levels between never and former smokers as well as between never and current smokers. In current smokers, duration in years had a higher correlation coefficient with urinary than blood cadmium levels. In contrast, number of cigarettes smoked per day had a higher correlation coefficient with blood than urinary cadmium levels. These data suggest that blood and urine cadmium biomarker levels differ by duration and dose. These findings should be considered in evaluating any association between cadmium and smoking related diseases, especially cardiovascular disease.     

中国沿岸小型鲸类误捕情况的调查

报道了１９９４ ～１９９５ 年对中国沿岸小型鲸类误捕情况的调查结果．调查表明,有误捕记录的小型鲸类主要有５ 种,即江豚（ Ｎｅｏｐｈｏｃａｅｎａ ｐｈｏｃａｅｎｏｉｄｅｓ） 、长喙真海豚（ Ｄｅｌｐｈｉｎｕｓ ｃａｐｅｎｓｉｓ） 、瓶鼻海豚（ Ｔｕｒｓｉｏｐｓｔｒｕｎｃａｔｕｓ） 、点斑原海豚（Ｓｔｅｎｅｌｌａ ａｔｔｅｎｕａｔａ） 和伪虎鲸（ Ｐｓｅｕｄｏｒｃａ ｃｒａｓｓｉｄｅｎｓ） ,其中以江豚的误捕率最高．１９９４ 年在山东、浙江、福建、广东、海南省和广西壮族自治区沿岸渔业误捕的小型鲸类约３０４５ ±２１２０ 头,其中江豚的误捕量约２１３２ ±１４８４ 头．误捕小型鲸类的渔具主要有拖网、刺网和张网．中国沿岸没有专门捕鲸的渔业方式．误捕的小型鲸类在沿岸居民的生活中不占重要地位,它们大部分被丢弃或廉价出售．目前尚不知道误捕对中国水域小型鲸种群影响的程度,但可能是造成数量下降原因之一．     

A survey of mammal diversity in the Turaif province,Kingdom of Saudi Arabia

One hundred and forty mammals of fifteen different species belonging to nine families, collected during one-year survey period in Turaif province of Saudi Arabia, are reported. Collections were made during studying the diversity of land vertebrate fauna of Turaif area at the northern province of Saudi Arabia. Cricetidae (n?=?64) was the most common family. Jaculus jaculus vocator (n?=?34) was recorded the highest number of events for any mammal in the surveyed area followed by Gerbillus nanus (n?=?23). Two species of mammals namely G. nanus (n?=?23) and Felis margarita (n?=?3) were reported for the first time in the study area. The geographical distribution of the collected species within this province was mapped.     

The stimulating effect of recombinant cytokine LIF on the mouse blastocysts during implantation

The effect of recombinant LIF cytokine (Leukemia inhibitory factor) on the isolated mouse embryos at the stages of middle and late blastocyst has been investigated. We have demonstrated here that this agent is necessary in vitro at the stage of normal trophoblast formation after the blastocysts hatch from zona pellucida. This cytokine (10 ng/ml) caused intensification of adhesion and proliferative activity of the trophoblast cells. This is important for intercellular interactions with endometrium and for invasion of embryos into the uterus. The recombinant LIF insignificantly influenced cells of the inner cell mass.     

A survey of therapeutic drug monitoring in a teaching hospital

ObjectivesTherapeutic drug monitoring (TDM) is one of the tools that aim to improve and ensure the best therapeutic effects while avoiding drug toxicity. This study aimed to identify the clinical utilization and application of TDM at a major teaching hospital in Jeddah.MethodsA cross sectional survey of the clinical utilization and application of TDM at King Abdulaziz University Teaching Hospital across nurses in medical, surgical, pediatric, and intensive care units. The sample size (n = 130) represented 30% of the nursing population. The collection of questionnaires started on the 31st of January 2019 and was completed by the 10th of March 2019.ResultsThe indication to use TDM was well-known to respondents. However, only 64% of respondents reported collection and measuring of the correct drug levels at a precise sampling time with no specific protocols being followed for each drug. Moreover, only 53% reported that the drug levels were being re-measured and adequately monitored for the right indication and proper sampling time. Regarding the presence of clinical pharmacists, 70% of the respondents indicated that no clinical pharmacist worked in their department.ConclusionResults demonstrate that appropriate sampling time was not used for the majority of monitored drugs. In the absence of a TDM request form, this finding probably indicates the lack of national or local TDM guidelines. In conclusion, TDM services, which include standardized forms, references, and an active clinical pharmacist will likely improve the application of TDM.     

陕西泾河流域2019年旧石器考古调查简报

泾河流域旧石器考古工作开展很早,1920年法国神父桑志华(E.Licent)在甘肃庆阳地区赵家岔、辛家沟黄土堆积中发现石英质石制品,标志着中国旧石器考古的开端。在之后的近100年时间里,学者们陆续在属于泾河流域的甘肃庆阳、平凉等地发现牛角沟、寺沟口、楼房子和陕西长武窑头沟、乾县大北沟等旧石器地点,这些发现丰富了泾河流域旧石器文化内涵。然而,上述发现多集中在泾河中上游,且开展时间较早,下游地区特别是关中平原几乎是一片空白。本次调查在泾河流域中下游新发现旧石器地点16处,集中分布在关中平原泾河及支流的黄土堆积上。在黄土堆积中获取石制品63件,类型包括石核、石片和刮削器等,属于北方地区石片石器工业。根据石制品埋藏层位及泾河流域地貌发育和地层对比,初步推测这些地点时代为晚更新世。     

Preliminary survey on the population and distribution of gibbons in yunnan province

Of the gibbons in China,Hylobates hoolock, H. lar, andH. concolor leucogenys are distributed only in Yunnan Province, whileH. c. concolor occurs limitedly in Yunnan Province and Hainan Island. During the periods from February to November 1984, and March to August 1985, the authors undertook surveys of the population and distribution of gibbons in 56 counties of 10 prefectures in Yunnan Province, and obtained the first reliable population and distribution data on the gibbons of this Province. The findings serve to fill an important blank in gibbon research in Yunnan and should pave the way for further research in the future.     

Effects of GnRH antagonist on endometrial protein profiles in the window of implantation

GnRH antagonists can suppress luteinizing hormone and follicle‐stimulating hormone (FSH), with less initial stimulatory effect and lower risk of ovarian hyperstimulation syndrome. The effects of GnRH antagonists on embryonic implantation remain controversial. To evaluate the effects of GnRH antagonists, endometrial tissues were biopsied from 12 women with intracytoplasmic sperm injection treatment, in which four subjects undergoing controlled ovulation stimulation with rFSH and GnRH antagonist, four subjects with a GnRH agonist long protocol, and four natural cycle controls. After iTRAQ quantification analysis, 24 proteins showed differential expression between natural cycle and agonist treatment group and 39 proteins between natural cycle and antagonist treatment group. A total of seven proteins demonstrated differential expression only in antagonist treatment group. Bioinformatic analysis implied these proteins can function in cell processes including angiogenesis, cell proliferation, apoptosis, cell migration, and immune response. Furthermore, GnRH antagonist suppressed the function of GNAS and ANPEP, which were important for endometrial functions. Immunohistochemical staining showed that ANPEP was mainly localized in the human endometrial stroma, while ACO2, CDC5L, and GNAS were mainly localized in the glands. This study could provide insights into the effect of GnRH antagonists on the endometrium, and help optimize the embryo implantation and improve the success rate for GnRH antagonist protocol.     

离子注入选育霉酚酸高产菌株及其发酵条件研究

离子注入技术是20世纪80年代兴起的一种综合诱变技术,其应用于生物工程已取得了丰硕成果,但在霉酚酸产生菌的诱变育种中的应用还未见报道。短密青霉菌(Penicillium brevicompactum)M_51是从土壤中分离得到的MPA产生菌F_663经过紫外线、微波等诱变处理得到的。为获得霉酚酸的高产工业菌株,进一步对该菌株进行了离子注入诱变处理。用15keV氮离子分5个剂量进行处理,结果显示,随离子注入剂量增加,存活率呈现较明显的下降_上升_下降的“马鞍型”变化趋势。在剂量为140×2.6×1013ions/cm2时,菌株变异率及正变率均最高,分别达到88.9%和63.4%。用HPLC定量测定发酵液中霉酚酸的含量,筛选到产霉酚酸能力提高30.1%的突变株M_163。经过连续传代试验,其遗传性状稳定。对发酵条件的优化结果显示最佳种龄为24h;用正交试验方法对发酵培养基中的碳、氮源进行优化,得到较优配方。突变株M_163在最优发酵条件下,霉酚酸摇瓶发酵单位可达2819μg/mL。野生菌株F_663的MPA产量为133μg/mL,经过5代诱变育种及发酵条件优化,产量提高了20.2倍。     

低能氮离子注入大肠杆菌诱发的生物学效应研究

研究离子注入的诱变和导入外源DNA的生物学效应,用低能氮离子注入处理大肠杆菌野生型菌株MC4100A,将含水母绿色荧光蛋白基因的质粒导入细胞中。实验结果表明,在一些转化子中绿色荧光蛋白的表达或折叠受到了影响,一些转化子丧失了分裂后分离能力,且细胞膜有一定程度的损伤,为进一步研究微生物细胞分裂和蛋白质折叠机理提供了菌株。故低能氮离子注入对微生物细胞的诱变效应和导入外源DNA效应的有机结合将使离子注入技术在生物学基础研究中有着广泛的应用前景。