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1.
Effect of long-term Se deficiency on the antioxidant capacities of rat vascular tissue 总被引:5,自引:0,他引:5
Selenium (Se) is an essential micronutrient in human health and Se deficiency has been incriminated in the etiology of cardiovascular
diseases. However, the effect of long-term Se deficiency on the antioxidant capacities of vascular tissue has not been elucidated.
This study was to determine whether long-term Se deficiency might affect the antioxidant capacity of rat vascular tissue and
whether the diet Se might affect the activities of glutathione peroxidase (GPx) and thioredoxin reductase (TR) in rat vascular
tissue. Weanling male Wister rats were fed Se-deficient and Se-adequate diets for 12 mo. Se was supplemented in drinking water
(1 μg Se/mL) for 1 mo. The arterial walls isolated from various groups were used in the assay. In comparison with the control,
Se-deficient rats exhibited significant decreases of GPx activity and total antioxidant capacity in the arterial wall. Similar
decreases appeared in the heart, liver, and kidney. The superoxide dismutase activity was also decreased in the Se-deficient
rat’s arterial wall. Followed by Se supplementation, they were restored to different extent. TR activity was decreased in
the heart, liver, and kidney, but increased in the arterial wall. The content of malondialdehyde was increased markedly in
Se-deficient rats. In conclusion, a positive correlation exists between dietary Se and antioxidant capacity of rat vascular
tissue except TR. It seems that the activities of GPx and TR in the rat arterial wall were mediated in different pathways
by the Se status. 相似文献
2.
Nitric oxide (NO) has been shown to both enhance hydrogen peroxide (H2O2) toxicity and protect cells against H2O2 toxicity. In order to resolve this apparent contradiction, we here studied the effects of NO on H2O2 toxicity in cultured liver endothelial cells over a wide range of NO and H2O2 concentrations. NO was generated by spermine NONOate (SpNO, 0.001–1 mM), H2O2 was generated continuously by glucose/glucose oxidase (GOD, 20–300 U/l), or added as a bolus (200 μM). SpNO concentrations between 0.01 and 0.1 mM provided protection against H2O2-induced cell death. SpNO concentrations >0.1 mM were injurious with low H2O2 concentrations, but protective at high H2O2 concentrations. Protection appeared to be mainly due to inhibition of lipid peroxidation, for which SpNO concentrations as low as 0.01 mM were sufficient. SpNO in high concentration (1 mM) consistently raised H2O2 steady-state levels in line with inhibition of H2O2 degradation. Thus, the overall effect of NO on H2O2 toxicity can be switched within the same cellular model, with protection being predominant at low NO and high H2O2 levels and enhancement being predominant with high NO and low H2O2 levels. 相似文献
3.
T. Rannem K. Ladefoged E. Hylander J. Hegnhøj S. Jarnum 《Biological trace element research》1993,39(1):81-90
Severe selenium (Se) depletion was found in nine patients receiving long-term home parenteral nutrition because of short bowel
syndrome. Plasma Se ranged from 0–0.51 (median 0.21 μmol/L) and erythrocyte Se ranged from 0.7–2.6 (median 1.8 μmol/gHgb),
which was significantly lower than in the controls. Glutathione peroxidase (GSHPx) in plasma and erythrocytes was also decreased.
After bolus injections with 200 μg Se/d in the form of sodium selenite for 4 mo, followed by 100 μg/d for 8 mo, plasma Se
increased to values slightly but significantly higher than in the controls. Erythrocyte Se reached normal levels in most of
the patients after 4 mo substitution, but it remained lower than in the controls. Following Se supplementation, plasma and
erythrocyte GSHPx did not differ between patients and controls. These data suggest that all patients receiving long-term parenteral
nutrition because of short bowel syndrome should receive at least 100 μg sodium selenite/d when given as bolus injections
to avoid Se depletion. 相似文献
4.
Cadmium induced lipid peroxidation (LPO) and the activity of antioxidantenzymes after the administration of a single dose of CdCl 2 (0.4 mg kg body wt, ip) was studied in rat erythrocytes.Cd intoxication increased erythrocyte LPO along with a decrease insuperoxide dismutase (SOD) up to three days of Cd treatment. Thedecrease in erythrocyte catalase (CAT) activity was marked within9 h of Cd intoxication. After three days of Cd treatment, LPOdecreased towards normal, along with an increase in erythrocyteSOC and CAT activity. Blood glutathione (GSH) decreased significantlywithin 24 h of Cd treatment, followed by an increase towards normal.Erythrocyte glutathione S-transferase (GST) activity increased up to10 days of Cd intoxication, probably in an attempt to reduce Cd toxicity.Serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase(SALP) and serum bilirubin increased up to 10 days of Cd intoxication.Blood urea increased significantly up to three days, followed by a decreasetowards normal. The results show that Cd induced LPO was associated with adecrease in antioxidant enzymes and GSH in erythrocytes; as these antioxidantsincrease in erythrocytes with recovery from Cd intoxication, the Cd inducedLPO reversed towards normal. The increase in the SGPT, SALP and serum bilirubincorrelated with LPO. The results suggest that Cd intoxication induces oxidativestress and alters the antioxidant system, resulting in oxidative damage torat erythrocytes. © Rapid Science 1998 相似文献
5.
Alfthan G Xu GL Tan WH Aro A Wu J Yang YX Liang WS Xue WL Kong LH 《Biological trace element research》2000,73(2):113-125
Keshan disease is a cardiomyopathy restricted to the endemic areas of China and seen in residents having an extremely low
selenium (Se) status. Prophylactic administration of sodium selenite has been shown to decrease significantly the incidence
of acute and subacute cases. The aim of the study was to assess the relative bioavailability of selenite versus organic Se-yeast
in a Se-deficient area in China with a randomized double-blind double-dummy design.
Healthy children (n=30) between 14 and 16 yr of age were randomized into three equal groups receiving either 200 μg/d selenite Se or 200 μg/d
Se-yeast or placebo for 12 wk. Blood was drawn at baseline, 4, 8, and 12 wk and 4 wk postsupplementation. The plasma Se concentration
(mean ± SD) was 0.16±0.03 μmol/L at baseline. Selenite and Se-yeast supplementation increased plasma Se to plateau values,
1.0±0.2 and 1.3±0.2 μmol/L, respectively. In red cells, Se-yeast increased the selenium level sixfold and selenite threefold
compared to placebo. The relative bioavailability of Se-yeast versus selenite measured as glutathione peroxidase (GSHPx) activity
was similar in plasma, red blood cells, and platelets. GSHPx activity reached maximal levels in plasma and platelets of 300%
and 200%, respectively, after 8 wk compared to the placebo group, but continued to increase in red cells for 16 wk.
Our study showed that although both forms of Se were equally effective in raising GSHPx activity, Se-yeast provided a longer
lasting body pool of Se. Se-yeast may be a better alternative to selenite in the prophylaxis of Keshan disease with respect
to building up of body stores. 相似文献
6.
Momčilo Mihailović Paul Lindberg Ivan Jovanović Duŝan Antić 《Biological trace element research》1992,33(1-3):71-77
The selenium (Se) contents in common cereals in endemic and nonendemic areas in Serbia are very low. Plasma Se levels of both
patients and healthy subjects, were also low, reflecting low Se intakes. Patients with Balkan endemic nephropathy (BEN) had
significantly lower (p<0.05) plasma Se levels than healthy individuals, both from regions close to endemic areas, and from Belgrade. Mean plasma
Se of BEN patients was slightly but insignificantly higher in samples taken immediately after dialysis than in those taken
before, suggesting that very little of the Se present in plasma is dialyzable. Plasma SeGSH-Px activities before and after
hemodialysis in both BEN and Nonendemic chronic renal failure (NCRF) patients were not significantly different, but BEN patients
had lower enzyme activities than those with NCRF and healthy controls. In BEN patients, a significant correlation between
plasma Se and SeGSH-Px activity was found.
NCFR patients were with diagnoses: TBC of kidneys, chronic glomerulonephritis, chronic pyelonephritis, and polycystic kidneys. 相似文献
7.
The relationship between the iodine intake level of a population and the occurrence of thyroid diseases is U-shaped. When
excessive iodine is ingested, hypothyroidism or hyperthyroidism associated with goiter might develop. The aim of the study
was to evaluate the effect of Se supplementation on the depression of type 1 deiodinase (D1) and glutathione peroxidase (GSHPx)
activities caused by excessive iodine. D1 activity was assayed by the method with 125I-rT3 as a substrate. Compared to the effect of iodine alone, iodine in combination with selenium increased the activities of D1
and GSHPx. The addition of selenium alleviated the toxic effects of iodine excess on the activities of D1 and GSHPx. 相似文献
8.
Selenium,zinc, and thyroid hormones in healthy subjects 总被引:3,自引:0,他引:3
Oliviero Olivieri Domenico Girelli Anna Maria Stanzial Luigi Rossi Antonella Bassi Roberto Corrocher 《Biological trace element research》1996,51(1):31-41
Iodothyronine 5′ deiodinase, which is mainly responsible for peripheral T3 production, has recently been demonstrated to be a selenium (Se)-containing enzyme. The structure of nuclear thyroid hormone
receptors contains Zinc (Zn) ions, crucial for the functional properties of the protein. In the elderly, reduced peripheral
conversion of T4 to T3 with a lower T3/T4 ratio and overt hypothyroidism are frequently observed. We measured serum Se and RBC GSH-Px (as indices of Se status), circulating
and RBC Zinc (as indices of Zn status), thyroid hormones and TSH in 109 healthy euthyroid subjects (52 women, 57 men), carefully
selected to avoid abnormally low thyroid hormone levels induced by acute or chronic diseases or calorie restriction. The subjects
were subdivided into three age groups. To avoid under- or malnutrition conditions, dietary records were obtained for a sample
of 24 subjects, randomly selected and representative of the whole population for age and sex. Low T3/T4 ratios and reduced Se and RBC GSH-Px activity were observed only in the older group. A highly significant linear correlation
between the T3/T4 ratio and indices of Se status was observed in the older group of subjects (r=0.54;p<0.002, for Se;r=0.50;p<0.002, for RBC GSH-Px). Indices of Zn status did not correlate with thyroid hormones, but RBC Zn was decreased in older as
compared with younger subjects. We concluded that reduced peripheral T4 conversion is related to impaired Se status in the elderly. 相似文献
9.
Charles Coudray Anne Marie Roussel Josiane Arnaud Alain Favier 《Biological trace element research》1997,57(2):183-190
Selenium (Se) and antioxidant vitamins might play an important role in the etiology of free radical-related diseases and aging.
In the édude de vieillissement artériel (EVA) study, we have determined the plasma thiobarbituric acid reacting substances
(TBARS) as an indicator of free radical-induced lipid peroxidation, plasma selenium and carotenoids, and erythrocyte vitamin
E levels in 1389 subjects aged 59–71 years. We also looked for an association between these parameters and cardiovascular
risk factors in early elderly. The results show that plasma TBARS were significantly increased in elderly in comparison with
values reported in younger adults. However, plasma Se and carotenoids as well as erythrocyte vitamin E in elderly people are
close to those reported in adult people. If plasma Se showed no difference between men and women, the three other parameters
were significantly higher in women than in men. With regard with cardiovascular risk factors, plasma TBARS were highly positively
correlated with total and low-density lipoprotein (LDL) cholesterol in men and women. Plasma carotenoids were also positively
correlated with plasma total cholesterol and LDL cholesterol in both sexes. Finally, plasma TBARS were highly correlated with
smoking and alcohol consumption. In conclusion, this part of the EVA study shows that some cardiovascular risk factors, like
smoking and cholesterol level, are associated with high free radical-induced TBARS levels in the preaging population, although
plasma Se and carotenoids as well as erythrocyte vitamin E levels in elderly people were close to those reported in adult
younger people. 相似文献
10.
Selenium and glutathione peroxidase mRNA in rat glioma 总被引:4,自引:0,他引:4
Zhang Z Miyatake S Saiki M Asahi M Yukawa H Toda H Kikuchi H Yoshimura SI Hashimoto N 《Biological trace element research》2000,73(1):67-76
11.
12.
Rats were subjected to bilateral carotid artery occlusion for 30 min, followed by reperfusion for varying time periods. The concentration of reduced and oxidized glutathione, glutathione peroxidase and glutathione reductase were determined in whole brain after varying periods of reperfusion. Lipid peroxidation was also assessed by determining the levels of malondialdehyde (MDA) in the brain. Reperfusion for 1 hr following bilateral carotid artery occlusion resulted in significant decrease in total glutathione (GSH) concentration along with small but significant increase in oxidized glutathione (GSSG) levels. After 4 hr of reperfusion, GSH levels recovered, although GSSG levels remained elevated up to 12 hr of reperfusion. Increase in malondialdehyde levels was also detected in the brain up to 12 hr of reperfusion. Glutathione reductase activity remained significantly low up to 144 hr of reperfusion, while glutathione peroxidase activity remained unaffected. These results demonstrate that oxidative stress is generated in the brain during reperfusion following partial ischemia due to bilateral carotid artery occlusion. 相似文献
13.
Lennart Hardell Marit Danell Carl-Axel Ängqvist Stefan L. Marklund Mats Fredriksson Anna-Lena Zakari Arne Kjellgren 《Biological trace element research》1993,36(2):99-108
Plasma selenium and glutathione peroxidase in erythrocytes were analyzed in a case-control study encompassing 441 cases with
breast cancer and 191 controls with benign breast disease. No difference in mean serum selenium level between cases and controls
on supplementary selenium intake was seen. If only individuals without supplementary intake, 278 cases and 135 controls, were
considered a preventive effect was found increasing with selenium level. This finding was significant among women 50 years
old or more with Mantel-Haenszel odds ratio=0.16 for individuals with serum selenium >1.21 μmol/L. Also for subjects with
serum selenium in the range 1.00–1.21 μmol/L a significant preventive effect was seen with odds ratio=0.38. For women under
50 years of age a nonsignificant preventive effect was seen. Glutathione peroxidase in erythrocytes did not correlate well
with serum selenium and was not a marker for the risk of breast cancer. 相似文献
14.
Selenium status is decreased in patients with intrinsic asthma 总被引:5,自引:0,他引:5
Jana Kadrabová Alexander Mad'arič Zuzana Kovačiková František Podivínsky Emil Ginter František Gazdík 《Biological trace element research》1996,52(3):241-248
Lowered selenium (Se) status has been observed in asthma patients. An increased production of reactive oxygen species (ROS)
owing to inflammatory condition has also been found in these patients and thus antioxidant properties of Se via glutathione
peroxidase (GPx) activity are of great importance. Concentrations of Se in plasma and erythrocytes as well as eryth-rocyte
GPx activity in 22 intrinsic asthma patients (five patients; all women were aspirin-sensitive) were compared with those of
33 control subjects. Se concentrations in both plasma and erythrocytes and GPx activity were decreased in intrinsic asthma
patients. There were no significant differences in investigated parameters of Se status between aspirin-tolerant and aspirin-intolerant
patients within intrinsic asthma group. Significantly high positive correlation between plasma and erythrocyte Se concentrations
was found when regarding all subjects as a whole. Se supplementation might be beneficial to patients with intrinsic asthma,
which may be at risk of Se deficiency. 相似文献
15.
16.
Tebekeme Okoko 《Journal of Genetic Engineering and Biotechnology》2018,16(2):485-490
The abilities of kolaviron and selenium (either separately or in combination) to prevent hydrogen peroxide-induced alterations in cell viability and activation were investigated. The cell line U937 was incubated with the antioxidants (i.e. kolaviron or selenium) for 24?h before exposure to hydrogen peroxide and cell viability was assessed via trypan blue dye exclusion assay. The U937 cells were also transformed to the macrophage form, incubated with the antioxidants before exposure to hydrogen peroxide. Subsequently, production of nitric oxide and pro-inflammatory cytokines were assessed as indices of macrophage activation. The myoblast cell line H9c2 was also incubated with Se and kolaviron for 24?h before exposure to hydrogen peroxide. Cell viability and generation of reactive oxygen species (ROS) were assessed via MTT and DCHF assays. The results revealed that hydrogen peroxide significantly reduced (p?<?0.05) the viability of U937 cells which was ameliorated by kolaviron and selenium. Kolaviron and selenium also reduced hydrogen peroxide-induced secretion of nitric oxide, TNF-α, IL-1 and IL-6 by transformed U937 cells. Hydrogen peroxide also significantly reduced (p?<?0.05) the viability of H9c2 cells which was significantly restored by kolaviron. Though selenium had no effect on the proliferation of H9c2 cells, co-treatment with kolaviron significantly reduced hydrogen peroxide-induced alterations. Both kolaviron and selenium also reduced hydrogen peroxide-mediated ROS production by H9c2 cells. In all cases, the combined action of kolaviron and selenium offered greater amelioration of the hydrogen peroxide-induced alterations than their separate effects (p?<?0.05) but may not be synergistic or additive. 相似文献
17.
Radiation is a promising and new treatment for restenosis following angioplasty. Nitric oxide has been proposed as a potential "anti-restenotic" molecule. We radiated the cultured rat vascular smooth muscle cells with Cobalt-60 gamma radiation at doses of 14 and 25Gy and observed nitrite production, cGMP content, L-arginine uptake, inducible nitric oxide synthase (iNOS) activity, and the gene expression of iNOS. Results showed that radiation at doses of 14 and 25Gy increased cGMP content by 92.4% and 86.4%, respectively. Radiation at the dose of 25Gy increased the iNOS activity and nitrite content, but radiation at the dose of 14Gy had no significant effect on iNOS activity and NO production. Both doses of radiation significantly decreased the L-arginine transport. Radiation at the doses of 14 and 25Gy increased iNOS gene expression significantly, which was consistent with the effect of radiation on iNOS activity. In conclusion, radiation induces the NO generation by up-regulating the iNOS activity. 相似文献
18.
Zagrodzki P Bartoń H Walas S Fołta M Stompór T Janusz-Grzybowska E Drozdz M Sułowicz W 《Biological trace element research》2007,116(1):29-41
We investigated the relations between selenium status (SeS) parameters, indexes of nutrition, erythropoiesis, and uremic toxemia,
serum electrolytes, and other biochemical markers in end-stage renal disease (ESRD) patients, as no multivariate statistical
analysis concerning all of these parameters was performed so far. SeS was evaluated by plasma Se concentration (plSe) and
glutathione peroxidase (plGSHPx) activity in 69 uremic patients treated with hemodialysis (HD) and 40 healthy controls. The
hierarchical multivariate partial least squares model (PLS2) was employed to establish data structure and correlations between
parameters investigated. plSe and plGSHPx activity were significantly lower in patients when compared with controls (p=0.000). plSe was positively associated with indexes of erythropoiesis and nutritional status, as well as serum electrolytes
and parameters of uremic toxemia. plGSHPx was inversely dependent on the pair of parameters: intact parathyroid hormone (iPTH)
and aluminum plasma concentration (Al). We conclude that (1) ESRD strongly decreases selenium status and (2) the PLS2 approach
revealed the existence of significant interactions among plSe, plGSHPx, and selected biochemical parameters or groups of such
parameters; some of these associations need further studies to be clarified. 相似文献
19.
Mihailović M Cvetković M Ljubić A Kosanović M Nedeljković S Jovanović I Pesut O 《Biological trace element research》2000,73(1):47-54
Placenta tissue may be a major source of lipid peroxidation products in pregnancy. It was proven that placental peroxidation
activity increases with gestation. Selenium (Se), as an essential constituent of glutathione peroxidase (GSH-Px), takes part
in the reduction of hydrogen peroxides and lipid peroxides. Malondialdehyde (MDA) is a major breakdown product split off from
lipid peroxides. In this study, Se and MDA content and GSH-Px activity were measured in blood and plasma taken from 20 apparently
healthy nonpregnant women between 19 and 38 yr of age and from 115 unselected pregnant women between 17 and 45 yr of age (35
in the first trimester, 22 in the second trimester, 38 in the third trimester, and 20 within 2 d of delivery). Samples of
umbilical cord blood and amniotic fluid were taken from women in the second and third trimesters and at delivery. The Se content
was measured by atomic absorption spectrometry (AAS), plasma MDA concentration by thiobarbituric acid reaction, and Se-dependent
GSH-Px spectrometrically. Blood and plasma Se contents of nonpregnant women were below those considered adequate, indicating
low selenium intake. In comparison to nonpregnant women, pregnant women had significantly decreased whole-blood and plasma
Se levels in the second and third trimesters and at delivery. The significant drop of whole-blood SeGSH-Px activity was observed
in the first trimester of pregnancy and its lower activity was maintained until delivery. A significant drop in plasma SeGSH-Px
activity occurred in the second trimester and attained the minimal level at delivery. The Se level and SeGSH-Px activity in
maternal and umbilical cord blood were at similar levels. Amniotic-fluid SeGSH-Px activity was nondetectable or exceptionally
low and its Se content remained unchanged during pregnancy. Plasma levels of MDA were significantly decreased in the second
and third trimesters and at delivery. The fetal blood plasma at birth had a lower MDA level compared to the levels of MDA
of their mothers at delivery. A low, but significant inverse correlation existed between blood SeGSH-Px activity and plasma
MDA content and between plasma Se and plasma MDA contents during pregnancy. A significant decrease of Se and SeGSH-Px activities
(antioxidant enzyme) in both blood and plasma suggests a possible drop in total antioxidant status during pregnancy. Elevated
MDA plasma levels might be the result of increased lipid peroxidation in placental tissue during pregnancy. 相似文献
20.
Effects of long-term selenium yeast supplementation on selenium status studied in the rat 总被引:1,自引:0,他引:1
Dietrich Behne Dorothea Alber Antonios Kyriakopoulos 《Journal of trace elements in medicine and biology》2009,23(4):258-264
To investigate the selenium status during long-term dietary supply of selenium yeast, 30-day-old male rats were fed for 379 days a methionine-adequate low-selenium diet supplemented with 0.2 mg Se/kg (selenium-adequate diet) or 1.5 mg Se/kg (high-selenium diet) in the form of selenium yeast that contained 60% of the element as l-selenomethionine. Their selenium load was determined at several intervals by neutron activation analysis of the selenium concentrations in the main selenium body pools, skeletal muscle and liver. After 64 days the tissue selenium concentrations plateaued in both groups and then stayed at that level. Compared with the selenium-adequate group, elevated tissue selenium concentrations were found in the high-selenium group, but the increase by a factor of 3.5 in the muscle and by a factor of 2.3 in the liver was smaller than the 7.5-fold increase in the selenium intake. In the selenium-adequate group about 50% of the muscle selenium and 30% of the liver selenium and in the high-selenium group about 85% of the muscle selenium and 70% of the liver selenium were estimated to be present in non-selenoprotein forms. During selenium depletion the liver glutathione peroxidase activity in the high-selenium group remained unaffected for 4 weeks and then decreased more slowly than that in the selenium-adequate group. From these results it can be concluded that selenium incorporated from the selenium yeast diet into non-selenoprotein forms can serve as an endogenous selenium source to maintain selenoprotein levels in periods of insufficient selenium supply. 相似文献