共查询到20条相似文献,搜索用时 46 毫秒
1.
Phosphorus Nutrition and the Intracellular Distribution of Inorganic Phosphate in Pea Root Tips: A Quantitative Study using31P-NMR 总被引:1,自引:0,他引:1
Pea plants (Pisum sativum L.) were supplied with external phosphatefor differing periods of time, so that their phosphorus statusvaried, and the intracellular distribution of inorganic phosphate(P1) in the roots was examined by 31P nuclear magnetic resonance.Over the range of phosphorus nutrition investigated, the quantityof vacuolar P1 per unit fresh weight of root tip changed considerably,whereas the quantity of cytoplasmic P1 per unit fresh weightof root tip did not alter. The relative volumes of the cytoplasmand the vacuole in pea root tips seemed to be little affectedby differences in phosphorus nutrition, and this implied thatthe concentration of P1 in the cytoplasm was kept almost constant,at a level estimated to be 18 mM. The rate of absorption of 32P-labelled phosphate was negativelycorrelated with the vacuolar P1 concentration, but there wasno clear correlation with the concentration of P1 in the cytoplasm. Key words: Compartmentation, Cytoplasm, Vacuole, Concentration, Absorption 相似文献
2.
Perennial ryegrass (Lolium perenne L.) was grown from seed for29 d in flowing solution culture containing 0.1, 0.4 or 6.4mmol m3 P before the concentrations were changed (0.1and 0.4 raised to 6.4; 6.4 lowered to 0.4; controls unchanged)for an experimental period of two weeks to test the hypothesisthat after the seedling stage, the maximum rate of plant growthcould be sustained by a lower concentration of phosphate atthe root/solution interface than was necessary for the maximumrate of seedling growth. During the 29 d seedling period growthwas greatest on 6.4 mmol m3 P achieving 179 mg per plantdry weight compared with 122 and 26 mg on 0.4 and 0.1 mmol m3P respectively. During the experimental period growth on thetreatment 6.4 lowered to 0.4 mmol m3 P continued at thesame rate as the 6.4 control achieving 981 and 983 mg per plantdry weight respectively. Similarly growth of the treatment 0.4raised to 6.4 mol m3 P was unaffected by the change inconcentration and was comparable with the 0.4 control. Bothresults support the hypothesis for seedlings exceeding about100 mg per plant dry weight. In contrast the small plants ofthe treatment 0.1 raised to 6.4 mmol m3 P behaved similarlyto seedlings and responded rapidly to the increased concentrationof phosphate in solution, achieving high rates of phosphateuptake and increasing the growth of shoot more than the growthof root so that the ratio of root: shoot declined from 065 to0.34, a value similar to that for the seedlings grown on 6.4mmol m3 P. Key words: Lolium perenne L, Phosphate concentration, Seedling growth 相似文献
3.
Studies on the Expansion of the Leaf Surface: II. THE INFLUENCE OF LIGHT INTENSITY AND DAYLENGTH 总被引:2,自引:0,他引:2
The parts played by constant amounts of visible radiation perday and its two componentsdaylength and intensityininfluencing the growth of Cucumis sativus have been investigated.The amount of radiation per day had a far greater influencethan either of its components per se. Nevertheless, small significanteffects of photoperiod were found, leaf expansion and dry weightincrease being greatest at daylengths between 10 and 15 hr.rather than with longer days which, with similar daily totals,would be expected to give the greatest amounts of assimilation. Rates of leaf production and appearance were greatest with thehighest amounts of radiation, but the rates of expansion ofindividual leaves and their maximum areas were greatest withintermediate amounts of radiation. This response resulted inan optimum curve relating the leaf surface and the dry weightattained after a given period to radiation. The amount of radiationgiving the maximum leaf surface and dry weight decreased withage and with external nutrient supply, but at any one age washigher for increase in dry weight than for increase in leafsurface; stem and root tissues responded more to high radiationthan did the leaf surface. The net assimilation rate was a linearfunction of visible radiation over the range of 15120cal. cm.-2 day-1 explored, the highest value of radiation usedrepresenting the intensity at which photosynthesis would beexpected to be maximal over a 1215 hr. day. The inhibitory effect of high radiation on leaf expansion andthe resultant influence on the growth of the plant are explainedin terms of the number and intensity of sinksfor carbohydrate and mineral nutrients within the plant. 相似文献
4.
Part I Tracer methods can yield valid results only if the behaviourof the tracer within the plant is indistinguishable from thatof the normal element. The extent to which radioactive isotopessatisfy this requirement must be considered from the followingviewpoints:
- their chemical and physical bahaviour before disintegration;
- the effects and substances formed by their disintegration;
- the effects of the radiation they emit.
5.
The development of the seminal root system, its ability to absorbnitrate, and effects on shoot growth were studied in barleyplants in nutrient solution. The roots received either a uniformsupply of 1.0 mM nitrate (controls), or a supply of the samesolution restricted to a 4-cm length of only one of the mainseminal roots (axes) on each plant, the remainder of the rootsystem receiving a solution containing a low concentration (0.01mM). Marked increases took place in both the growth of lateralroots and the absorption and transport of 15N-labelled nitrate(per unit root weight) from the zone locally supplied with 1.0mM nitrate. These effects appear largely to compensate for thedeficient supply of nitrate to the remainder of the root system,since after 14 d the relative growth rate (g g1d1)of the total plant equals that of the controls. Rates of 15N-nitrateuptake (per unit root weight) remain relatively uniform throughoutthe 29-d experiment, during which root axes develop from theirinitial unbranched form to a complex system of laterals. Theresults are discussed in relation to possible mechanisms bywhich coordination is maintained between root growth, ion uptake,and shoot growth. 相似文献
6.
Physiological changes in, and phosphate uptake by potato plants during development of, and recovery from phosphate deficiency 总被引:6,自引:0,他引:6
The development of phosphate deficiency (P-stress) was observed in rooted sprouts of Solanum tuberosum L. cv. Desiree growing in solutions without phosphate. Shoot growth was inhibited by P-stress within 3 to 5 days of terminating the phosphate supply, while significant effects on root growth were not recorded until 7 to 9 days. Thus, the shoot:root dry weight ratio decreased from 4.3 to 2.6 over a 10-day period. Growth in the absence of an exogenous phosphate supply progressively diluted the phosphorus in the plant. The proportional decrease in concentration was similar in roots and shoots over a 7-day period, even though the former were growing more quickly. The potential for phosphate uptake per unit weight of root increased rapidly during the first 3 days of P-stress. When the plants were provided subsequently with a labelled, 1 mol m?3 phosphate solution, the absorption rate was 3 to 4-fold greater than that of control plants which had received a continuous phosphate supply. The increased rate of uptake by P-stressed plants was accounted for by an increase (3-fold) in the Vmax of system 1 for phosphate transport and by a marked increase in the affinity of the system for phosphate (decrease in Km). In the early stages of P-stress, before marked changes in growth were measured, the proportion of labelled phosphate translocated to the shoots increased slightly relative to the controls when a phosphate supply was restored. In the later stages of stress a greater proportion was retained in the root system of P-stressed plants than in that of controls. In plants with roots divided between solutions containing or lacking a phosphate supply, the increased absorption rate was determined by the general demand for phosphate in the plant and not by the P-status of the particular root where uptake was measured. By contrast, the poportion translocated was strongly dependent on the P-status of the root. The restoration of a phosphate supply to P-stressed plants was marked by a rapid increase in the P concentration in snoots and roots which returned to levels similar to unstressed controls within 24 h. The enhanced uptake rate persisted for at least 5 days, resulting in supra-normal concentrations of P in both shoots and roots, and in the formation of extensive necrotic areas between the veins of mature leaves. Autoradiographs showed accumulations of 32P in these lesions and at the points where guttation droplets formed on leaves. 相似文献
7.
Summary Measurements were made of the diffusion of P32-labelled phosphate to single roots of onion, leek and rye-grass growing in an Upper Greensand sandy loam (UGS) and a Coral
Rag Clay (CRC) to which different amounts of phosphate had been added. Concentration-dependent diffusion coefficients for
phosphate ions in the soils were calculated from phosphate desorption isotherms in calcium chloride. The experimental uptake
by roots of known dimensions was compared with supply expected by diffusion to a cylindrical model root of the same dimensions.
Allowance was made for absorption by the root hairs on rye-grass roots.
Phosphate absorption by a cm length of intact root was found to continue for at least 16 days for onion, 10 days for leek
and 5 days for rye-grass. Over a wide range of conditions (phosphate concentrations, soils, plant species), experimental uptake
was close to the maximum calculated to be possible for the diffusion model except on one soil at a high level of phosphate.
Although the concentration of phosphate in the soil solution at the root boundary appeared to be reduced to a small fraction
of the initial concentration, because of the extreme non-linear form of the desorption isotherm less than 1/2 of the P32 exchangeable pool of P was considered to contribute to diffusion.
Phosphate uptake by rye grass could only be accounted for if the root hairs were active. Although only a small fraction of
the uptake is derived from inside the root hair cylinder, this increases the efficiency of the central root 2.3 fold by providing
a zone close to the central root through which phosphate moves very readily. 相似文献
8.
The effects of the phosphate status of young barley plants andof respiratory inhibitors on the distribution of phosphate absorbedduring experimental periods lasting 24 hours has been studied. The pretreatment of plants with 10 p.p.m. P increases the upwardmovement of phosphate when the plants are subsequently treatedwith0·001 p.p.m. P. No such effect occurs when 10 p.p.m.P are supplied. Sodium azide, 2:4-dinitrophenol, and diethyldithiocarbamatereduce the absorption of phosphate when the concentration ofthe external solution is 10 p.p.m. When, however,0·p.p.m.P is provided, contrasting results are obtained, the most strikingfeature being that a considerable increase in the content ofshoots may be induced. The extent of this effect is variable.A series of experiments with 103 M. azide indicates thatthe extent to which shoot content is increased depends on theextent to which phosphate is retained in the roots in the absenceof the inhibitor. These results suggest that the small amount of absorbed phosphatefound in the shoots of plants supplied with low concentrationsof phosphate is due to the metabolic retention of phosphatein the roots. It appears that two apparently distinct activeprocesses in roots are subject to inhibition by the three inhibitorsemployed, namely metabolic retention and the transfer of ionsacross the root to the stele. The relative extent to which theseprocesses are inhibited depends on the condition of the plants;metabolic retention may be reduced without comparable effectson absorption. It is considered that these results are incompatiblewith the hypothesis that absorption is directly mediated bythe electron transfer in respiration. An alternative mechanismis discussed. 相似文献
9.
Developmental Physiology of Sugar Beet: I. THE INFLUENCE OF LIGHT AND TEMPERATURE ON GROWTH 总被引:1,自引:0,他引:1
Sugar beet plants were grown for 12 weeks from emergence ingrowth rooms at temperatures of 10, 17, 24 and 31 °C and20, 50, 80, and 110 cal visible radiation cm-2d-1, and the changeswith time in their dry weight, leaf area, leaf numbers, andstorage root sugar determined. The first stage of growth wasdominated by the development of the shoot, but the storage rootgradually assumed increasing importance and eventually grewat a faster rate and to a greater weight than the shoot. Therelative growth rate and final yield of dry matter of the shootwere greatest at 24 °C and of the root between 17 and 24°C. The relative rate of expansion and the final area ofthe leaf surface were also greatest at 24 °C, whilst therates of production and of unfolding of leaves were greatestat about 17 °C. All these attributes were increased withincreased radiation. Net assimilation rate increased almostproportionately with radiation and was not significantly affectedby temperature.The relationships of total leaf area with plantdry weight, root dry weight with shoot dry weight, and totalleaf number with plant dry weight were scarcely affected bychanges in radiation, but were much influenced by temperature.Plants of the same dry weight generally had bigger roots andsmaller areas of leaf surface as temperatures departed from24 °C and had most leaves at 17 °C. Sugar concentrationsin the storage root were greatest at 17 °C, but the totalamount of sugar was about the same at 17 and 24 °C. Theconcentration of sugar in the storage root depended on rootsize.Thus, temperature affected both the rate and pattern ofdevelopment, and radiation affected the rate but not the patternof development. 相似文献
10.
Responses to a localized supply of phosphate were studied inbarley grown in continuous flow solution culture. Root systemswere either uniformly supplied with 50 µM phosphate (controls)or the same solution was supplied to only a 4 cm or 2 cm lengthof a seminal root (localized supply), the remainder of the rootsystem receiving a nutrient solution lacking phosphate. Little development of laterals occurred on those parts of theroot system receiving no phosphate from the external solution,while an increase in the number and extension of laterals tookplace in the 4 cm zone enriched with phosphate. Compared withsimilar zones on controls, the total length of laterals wasincreased 15-fold in 21 d plants. In addition, rates of 32P-phosphateuptake and translocation to shoots per unit root weight werehigher than in controls by a factor of 2?55?0. Furtherincreases in the growth of lateral roots, and rates of phosphateuptake, were induced when the segment initially supplied withphosphate was restricted to only 2 cm. These localized modifications to root growth and uptake of phosphatelargely compensated for the deficient supply of phosphate tothe remainder of the root system. After an early period of retardedgrowth and phosphate stress, the relative growth rate of plantsand the concentration of phosphate in shoots were restored tolevels similar to that of the controls. The manner in which the supply of phosphate may control rootdevelopment, and the nature of the co-ordination between rootgrowth, phosphate uptake, and shoot growth, are discussed. 相似文献
11.
Sunflower root meristems are composed of two populations ofcells which respond differently to stress. One population becomesarrested in G1 and G2, while the second leakypopulation (0.251.0 per cent) is able to pass throughS even during carbohydrate starvation. Leaky cells enter S ata rate of 0.06 per cent cells h1 after 48 h of starvation.The character of leakiness is retained by roots starved fortwo successive 48 h starvation periods separated by an 8 h sucrosepulse. Single and double layer autoradiograph experiments demonstratedthat leaky cell progeny maintain their leaky character throughat least two cell generations. Leaky cells are located at randomin the root cap, promeristem, ground meristem, protoderm, cortex,and pericycle. The presence of leaky cells may indicate a stressresponse mechanism to repopulate the root meristem. 相似文献
12.
With a view to finding a means of reducing contamination offoods by radiostrontium, the inhibiting effects of various anionson the transport of strontium in plants are investigated. Pea and bean plants were pretreated with silicate, phosphateand nitrate ions, and the distribution of strontium in variousparts of the plants was examined.
- In the case of pretreatment with silicate or phosphate ions,strontium absorption by the plant was decreased even if theseanions were absent in the culture solution at the time of applicationof 89Sr.
- The absorption of strontium by the plants pretreatedwith nitrateions was similar to that of the controls.
- Strontiumabsorbed by the plants pretreated with silicate orphosphatewas found to be accumulated in the roots and its quantitytransportedto the aboveground parts was remarkably small; especiallyinthe case of application of silicate, no measurable amountofstrontium was transported to the upper parts of the plant.
- Inthe control and nitrate-pretreated plants, strontium absorbedin the roots was readily transported to the aboveground parts.
13.
BREEZE V. G.; WILD A.; HOPPER M. J.; JONES L. H. P. 《Journal of experimental botany》1984,35(8):1210-1221
The effect of phosphate concentration in flowing solution cultureat a range between 0.04 and 32 mmol m3 P on the growthof perennial ryegrass was studied in two experiments, each lastingabout 45 d after sowing. Phosphorus contents of seedlings wereaffected by the concentration in solution within about 5 d fromgermination, and dry weight differences were first observedat about 6 d after this. The rate of uptake of phosphate byseedlings was affected by the concentration in solution beforethe root fresh weight or root/emdash shoot ratio had changed.Young plants (less than 4 weeks old) were more sensitive tophosphate concentration in solution than older ones. In conditionsof high rate of growth, older plants required a solution concentrationbetween 0.1 and 0.4 mmol m3 P to achieve maximum potentialgrowth rate, whereas for plants of similar age but less dryweight, 0.04 mmol m3 P was adequate. Towards the endof the experimental period, plants growing at a nominal solutionconcentration of 0.04 mmol m3 P were able to obtain phosphatefrom a solution of about 0.01 mmol m3P. Phosphate toxicity was not observed, nor were there visual symptoms(other than reduced growth) of phosphate deficiency in plantswhose growth was limited by phosphate concentration in solution. Key words: Lolium perenne, Phosphate uptake 相似文献
14.
When 32P and 45Ca were supplied simultaneously to bean plantsgrowing in culture solution under controlled conditions thatfavoured rapid transpiration it was found that after one hour7.9 per cent of the 32P taken up had penetrated into the stem,while the 45Ca present was still in the root. There was a similardelay in the movement of 45Ca from stem to leaves. Althoughmovement in the transpiration stream may be one important mechanismfor translocation, it is apparent that the distribution of theseelements is greatly affected by selective exchange reactionsand differential absorption in transit. 相似文献
15.
Uptake of Phosphate by White Clover: II. THE EFFECT OF PH ON THE ELECTROGENIC PHOSPHATE PUMP 总被引:1,自引:0,他引:1
The effect of pH on the electrogenic phosphate pump in whiteclover roots was investigated. It was found that the pump operatedbetween pH 3.5 and 8.0 with an optimum at 4.3. External pH changesaffected phosphate uptake and PD to the same extent so thata constant relationship between them was maintained. The activityof the pump declined when the carbohydrate supply was cut offby excision of the root or defoliation of the plant but couldbe restored by lowering the pH around the root. It was concludedthat the electrogenic phosphate pump could be driven by a pHgradient across the cell membrane. However, there did not appearto be a direct relationship between the operation of the pumpand extrusion of H+ by the root. 相似文献
16.
During vegetative growth in controlled environments, the patternof distribution of 14C-labelled assimilates to shoot and root,and to the meristems of the shoot, was measured in red and whiteclover plants either wholly dependent on N2 fixation in rootnodules or receiving abundant nitrate nitrogen but lacking nodules. In experiments where single leaves on the primary shoot wereexposed to 14CO2, nodulated plants of both clovers generallyexported more of their labelled assimilates to root (+nodules),than equivalent plants utilizing nitrate nitrogen, and thiswas offset by reduced export to branches (red clover) or stolons(white clover). The intensity of these effects varied with experiment.The export of labelled assimilate to growing leaves at the terminalmeristem of the donor shoot was not influenced by source ofnitrogen. Internode elongation in the donor shoot utilized nolabelled assimilate. Whole plants of white clover exposed to 14CO2 on seven occasionsover 32 days exhibited the same effect on export to root (+nodules),which increased slightly in intensity with increasing plantage. Nodulated plants had larger root: shoot ratios than theirequivalents utilizing nitrate nitrogen. Trifolium repens, Trifolium pratense, red clover, white clover, nitrogen fixation, nitrate utilization, assimilate partitioning 相似文献
17.
设置不同的Al 3+浓度(0、25、50、100、200、400 μmol·L-1)和培养时间 (12、24 h),研究了边缘细胞活性和大豆(Glycine max)根中 过氧化氢酶(CAT)、过氧化物酶POD)、超氧化物歧化酶SOD)随Al 3+浓度及处理时间变化的规律,并通过Hoec hst333 42-PI双重荧光染色、 梯状DNA(即DNA ladder)分析和末端脱氧核糖核酸转移酶介导的dUTP切口末端标记(即TUNEL原位标记)检测,研究了Al 3+对大豆根边缘细胞 程序性死亡诱导的生理生态作用。结果表明,Al 3+胁迫能诱导边缘细胞的死亡,随着Al 3+浓度的升高和处理时间的延长,细胞死亡率增加。通 过Hoechst33342-PI双重荧光染色、DNA ladder分析和TUNEL原位标记,检测到Al 3+胁迫下发生程序性死亡的边缘细胞。其表现为:在 400μmol·L-1 Al 3+诱导大豆根24 h时, 核酸电泳显示细胞DNA发生特异性降解并形成阶梯状电泳条带(DNA ladder),用TUNEL原位标记检测200 和400μmol·L-1 Al 3+处理12 h后的大豆根 边缘细胞,发现DNA的3′-OH端被原位特异标记,二氨基联苯胺(DAB)显色后,细胞核为阳性或强 阳性。同时,高浓度Al 3+ (>100μmol·L-1)处理下,CAT、POD和S OD活性均有不同程度的下降,CAT和SOD的活性也随处理时间的延长而降低 。说明在Al 3+胁迫下边缘细胞的死亡可能是一种程序性死亡形式,高浓度Al 3+胁迫下,通过诱导活性氧在细胞体内的产生和累积而导致细胞凋 亡,此过程是其对逆境胁迫所作出的生理生态防御性应答方式之一。 相似文献
18.
准噶尔荒漠分布的早春短命植物不仅具有十分独特的生物学特点,而且在荒漠植物群落演替、物种多样性维持及土壤改良与防治水土流失等方面具有重要的生态学价值。该文运用Li-6400开放式气体交换光合作用测定系统,对分布于准噶尔荒漠的16种早春短命植物生长盛期的净光合速率(Pn)、蒸腾速率(Tr)、水分利用效率(WUE)等特征进行了测定,并对其中7种植物与生长相关的生物量分配特征进行了分析。结果表明:1)16种植物的最大Pn、 最大Tr及WUE分别为8.07~35.96 μmol CO2·m-2·s-1、3.16~29.64 mmol H2O·m-2·s-1、0.54~4.26 μmol CO2·mmol-1H2O;种间最大Pn与最大气孔导度(Stomatal conductance, Gs)之间存在正相关关系,其相关系数为0.77(p<0.05),线性回归斜率为26.36 μmol·mmol-1;从光合速率对胞间CO2浓度及光量子通量密度的响应曲线来看,这类植物的表观CO2补偿点均在4~5 Pa之间(28~30 ℃),表观羧化效率为0.64~1.86 μmol CO2·m-2·s-1·Pa-1,表观量子效率为0.05~0.06。2)从生物量分配来看,所测植物的个体生物量为0.05~0.39 g;单株总叶面积为 3.24~51.40 cm2;单位叶面积干重为0.40~0.77 g·m-2,根在总生物量中所占比例为5.72%~19.43%,单株叶面积比在2.92~9.00 m2·kg-1之间。种间根所占生物量的比与对应的WUE之间的比较分析结果表明,二者之间存在显著的正相关关系,其相关系数r为0.93(p<0.01)。这些结果表明,所观测的早春短命植物具有典型的C3植物特征,相比其它类型的荒漠植物具有较高的单位叶面积Pn、高Tr及低WUE,并且在生长发育过程中表现出很低的根/地上生物量比、较高的叶面积比和单位叶面积干重,说明它们具有相对高的生长速率,这与其生长发育节律相一致,反映了它们与准噶尔荒漠环境相适应的特点。 相似文献
19.
In controlled environment experiments, the uptake and distributionpatterns of 32P were studied in relation to flowering and fruitingin the cowpea. Absorption by the plant, retention by the fedleaf, and translocation to various plant parts depended on theposition and age of the fed leaf. At the 4-leaf stage, whenthe terminal leaflet of the first trifoliate (oldest) was fed32P, the plant absorbed 43 per cent of total radioactivity suppliedin 24 h. The percentages resulting from the feeding of trifoliates2, 3, and 4 (youngest) were 58, 71, and 73 respectively. Trifoliate1 retained 34 per cent of the total radioactivity, while trifoliate4 retained 68 per cent. Of the plant tissues, the root accumulatedthe greatest amounts of the P exported by the fed leaflet, withthe feeding of trifoliates 1, 2, or 3. With the feeding of trifoliate4, however, the root imported only 0.2 per cent of total radioactivity. When the plants had one set of flowers fully open at raceme1, the flowers imported 0.19 per cent of total radioactivityin the cultivar Adzuki, and 0.36 per cent in the cultivar EarlyRamshorn. When the raceme 1 flowers developed into fruits whilethe raceme 2 flowers were fully open, the raceme 1 fruit importof radioactivity was 2.61 per cent in Adzuki and 14.21 per centin Early Ramshorn. Raceme 1 fruits of Early Ramshorn thus accumulatedfive times as much P radioactivity as those of Adzuki. Whenracemes 1 and 2 both bore fruits, removal of the raceme 1 fruitsjust prior to 32P feeding, led to the import by the raceme 2fruits of 0.25 per cent in Adzuki and 0.09 per cent in EarlyRamshorn. The raceme 1 fruits constituted a more potent sinkfor 32P in Early Ramshorn than in Adzuki. The results are discussedin relation to the known patterns of premature abscission offlowers and young fruits in the two cultivars. 相似文献
20.
研究了黑麦、玉米在经甲基胺草膦和Atrazine两种除草剂处理后叶绿素含量、叶绿体和根尖分生组织内的蛋白质组份变化,实验结果表明,0.1 mg·L-1的Atrazine可使黑麦中的叶绿素含量下降(分别从对照的1.72±0.034 mg·g-1 FW下降到1.62±0.05 mg·g-1 FW、1.25±0.015 mg·g-1 FW)。2种除草剂均可使黑麦、玉米中的蛋白质组份产生改变,如当分别用0.1 mg·L-1的Atrazine处理时,黑麦分生组织中,有4个蛋白质斑点,斑点7、斑点18~20被诱导产生,12个蛋白质斑点,斑点6、斑点8~17、斑点21消失。玉米分生组织中,有4个蛋白质斑点,斑点5、斑点14~16被诱导产生,4个蛋白质斑点,斑点17~20消失。黑麦叶绿体中,有两个蛋白质斑点被诱导产生,13个蛋白质斑点,斑点1~13消失,但Atrazine处理不引起玉米叶绿素含量和叶绿体蛋白质组份的变化。4 mg·L-1 APM可引起黑麦和玉米分生组织蛋白质组份变化,在黑麦中,1个蛋白质斑点被诱导产生,4个蛋白质斑点,斑点2~5消失。玉米分生组织中,15个蛋白质斑点,斑点4~5、斑点7~16、斑点21~23被诱导产生,4个蛋白质斑点,斑点1~3、斑点6消失。APM均不能引起2种作物中叶绿素含量和叶绿体蛋白质组份的变化。 相似文献