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Searching for DNA-protein interactions by lambda phage display 总被引:5,自引:0,他引:5
Cicchini C Ansuini H Amicone L Alonzi T Nicosia A Cortese R Tripodi M Luzzago A 《Journal of molecular biology》2002,322(4):697-706
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M West J Mikovits G Princler Y L Liu F W Ruscetti H F Kung 《The Journal of biological chemistry》1992,267(35):24948-24952
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Using a phylogenetic approach, we identified highly conserved sequences within intron 3 of the human TNF-alpha gene. These sequences form cell type-specific DNase I hypersensitivity sites and display cell type-specific DNA-protein contacts in in vivo genomic footprints. Consistent with these results, intron 3 confers specific activity upon a TNF-alpha reporter gene in Jurkat T cells, but not THP-1 monocytic cells. Thus, using a combinatorial approach of phylogenetic analysis, DNase I hypersensitivity analysis, in vivo footprinting, and transfection analysis, we demonstrate that intronic regulatory elements are involved in the cell type-specific regulation of TNF-alpha gene expression. 相似文献
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