共查询到20条相似文献,搜索用时 15 毫秒
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βig-h3, an extracellular matrix protein involved in various biological processes including cellular growth, differentiation, adhesion, migration, and angiogenesis, has been shown to be elevated in various inflammatory processes. Death receptor 3 (DR3), a member of the TNF-receptor superfamily that is expressed on T cells and macrophages, is involved in the regulation of inflammatory processes through interaction with its cognate ligand, TNF-like ligand 1A (TL1A). In order to find out whether the TL1A-induced inflammatory activation of macrophages is associated with the up-regulation of βig-h3 expression, the human acute monocytic leukemia cell line (THP-1) was stimulated with either recombinant human TL1A- or DR3-specific monoclonal antibodies. Stimulation of DR3 up-regulated the intracellular levels as well as the secretion of βig-h3. Utilization of various inhibitors and Western blot analysis revealed that activation of protein kinase C (PKC), extracellular signal-regulated kinase (ERK), phosphoinositide kinase-3 (PI3K), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is required for TL1A-induced βig-h3 expression. PKC appears to be the upstream regulator of PI3K since the presence of PKC inhibitor blocked the phosphorylation of AKT without affecting ERK phosphorylation. On the other hand, suppression of either PI3K or ERK activity resulted in the suppression of IκB phosphorylation. These findings indicate that TL1A can regulate the inflammatory processes through modulation of the βig-h3 expression through two separate pathways, one through PKC and PI3K and the other through ERK, which culminates at NF-κB activation. 相似文献
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Christian Lavedan Elisabeth Leroy Anindya Dehejia Stephanie Buchholtz Amalia Dutra Robert L. Nussbaum M. H. Polymeropoulos 《Human genetics》1998,103(1):106-112
We have identified and characterized a new member of the human synuclein gene family, γ-synuclein (SNCG). This gene is composed
of five exons, which encode a 127 amino acid protein that is highly homologous to α-synuclein, which is mutated in some Parkinson’s
disease families, and to β-synuclein. The γ-synuclein gene is localized to chromosome 10q23 and is principally expressed in
the brain, particularly in the substantia nigra. We have determined its genomic sequence, and established conditions for sequence
analysis of each of the exons. The γ-synuclein gene, also known as BCSG1, was recently found to be overexpressed in advanced
infiltrating carcinoma of the breast. Our survey of the EST database indicated that it might also be overexpressed in an ovarian
tumor.
Received: 6 February 1998 / Accepted: 8 April 1998 相似文献
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Reparative dentin has a wide variety of manifestations ranging from a regular, tubular form to an irregular, atubular form.
However, the characteristics of reparative dentin have not been clarified. This study hypothesized that the level of bone
sialoprotein (BSP) expression will increase if the newly formed reparative dentin is bone-like but the dentin sialophosphoprotein
(DSPP) level will decrease. In order to test this hypothesis, the expression of BSP and DSP was examined by immunohistochemistry
and the expression of BSP was measured by in situ hybridization in an animal model. The pulps of 12 maxillary right first
molars from twelve male rats were exposed and capped with MTA. In addition, in order to understand the role of transforming
growth factor-beta 1 (TGF-β1) during reparative dentinogenesis, the expression of BSP and DSPP mRNA was analyzed by RT-PCR
in a human dental pulp cell culture, and the transforming growth factor-beta 1 receptors (TβRI) and Smad 2/3 were examined
by immunofluorescence in an animal model. DSP was expressed in the normal odontoblasts and odontoblast-like cells of the reparative
dentin. Interestingly, BSP was strongly expressed in the odontoblast-like cells of reparative dentin. The level of the TβRI
and Smad 2/3 proteins was higher in the reparative dentin than in the normal dentin. TGF-β1 up-regulated BSP in the human
pulp cell cultures. This suggests that reparative dentin has both dentinogenic and osteogenic characteristics that are mediated
by TGF-β1. 相似文献
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Jana Hujová Jakub Sikora Robert Dobrovolny Helena Poupětová Jana Ledvinová Marta Kostrouchová Martin H?ebí?ek 《BMC cell biology》2005,6(1):5
Background
Human α-galactosidase A (α-GAL) and α-N-acetylgalactosaminidase (α-NAGA) are presumed to share a common ancestor. Deficiencies of these enzymes cause two well-characterized human lysosomal storage disorders (LSD) – Fabry (α-GAL deficiency) and Schindler (α-NAGA deficiency) diseases. Caenorhabditis elegans was previously shown to be a relevant model organism for several late endosomal/lysosomal membrane proteins associated with LSDs. The aim of this study was to identify and characterize C. elegans orthologs to both human lysosomal luminal proteins α-GAL and α-NAGA. 相似文献14.
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Partial structure of the human α2(IV) collagen chain and chromosomal localization of the gene (COL4A2) 总被引:3,自引:0,他引:3
Paul D. Killen Clair A. Francomano Yoshihiko Yamada William S. Modi Stephen J. O'Brien 《Human genetics》1987,77(4):318-324
Summary We have isolated a 2.1-kb cDNA clone from a human placental library encoding part of the 2 chain of collagen IV, a major structural protein of basement membranes. The DNA sequence encodes 446 amino acids in the triplehelical domain plus the 227 amino acids of the carboxy-terminal globular domain. The latter structure is composed of two homologous subdomains and is highly conserved between the 1 and 2 chains. The triple-helical domain contained seven interruptions of the Gly-X-Y repeat and these interruptions were in general larger than their counterparts in the 1 chain. DNA from human rodent hybrid cell lines was analyzed under conditions in which there was no cross-hybridization of the 2(IV) cDNA probe with the gene for the 1(IV) collagen chain. An Eco RI fragment characteristic of the 2 chain had a concordance of 0.97 with chromosome 13. This result was confirmed and extended with in situ localization of the gene at 13q34. Since the 1(IV) gene has previously been localized to 13q34, the two type IV collagen genes reside in the same chromosome region (13q34), possibly in a gene cluster. The presence of the genes for type IV collagen chains on chromosome 13 excludes a primary role for these genes in adult polycystic kidney disease and X-linked forms of hereditary nephritis. 相似文献
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<正>Transforming growth factorβ(TGF-β) regulates various physiological processes such as cell proliferation, differentiation, morphogenesis, andt tissue homeostasis and regeneration. The deregulation of these processes leads to many types of human diseases such as cancer (Massagué,2008). TGF-βsignaling is initiated upon active TGF-βdimer 相似文献