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Sathiyaraj Srinivasan Jae-Jin Lee Sang-Seob Lee Myung Kyum Kim 《Current microbiology》2014,69(3):263-269
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated as SWU8T, was isolated from a mountain soil collected in Seoul Women’s University campus at South Korea. Phylogenic analysis, using 16S rRNA gene sequence of the new isolate, showed that strain SWU8T belongs to the genus Pontibacter. The highest sequence similarities were 96.2 % with Pontibacter saemangeumensis GCM0142T, 95.5 % with Pontibacter toksunensis ZLD-7T, 95.3 % with Pontibacter roseus DSM 17521T, and 95.1 % with Pontibacter odishensis JC130T. Chemotaxonomic data showed that the most abundant fatty acids were summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B; 26.9 %), iso-C15:0 (25.6 %), and iso-C17:0 3OH (10.6 %), and major polar lipid was phosphatidylethanolamine. The DNA G+C content of strain SWU8T was 48.5 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain SWU8T presents a novel species of the genus Pontibacter, for which the name Pontibacter humi sp. nov. is proposed. The type strain is SWU8T (=KEMC 9004-131T = JCM 19178T). 相似文献
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Two bacterial isolates from soil samples taken in Korea, strains YM2-7T and WD2-19T, were characterized using a polyphasic approach. The cells were strictly aerobic, Gram-positive, motile with peritrichous
flagella, and rod-shaped. Both strains formed ellipsoidal bulging positioned subterminal spores. Phylogenetic analysis of
their 16S rRNA gene sequences revealed a clear affiliation with the Firmicutes. The 16S rRNA gene sequence similarity between YM2-7T and WD2-19T was 96.5%. Strains YM2-7T and WD2-19T showed 16S rRNA gene sequence similarities of 93.0–96.5% to type strains of recognized Cohnella species. The G+C contents of the DNA of strains YM2-7T and WD2-19T were 52.2 and 55.6 mol%, respectively. The major fatty acids of strains YM2-7T and WD2-19T were anteiso-C15:0 (44.4%), C16:0 (19.2%), and iso-C16:0 (16.8%) and anteiso-C15:0 (46.5%), iso-C16:0 (21.8%), and C16:0
(11.2%), respectively. Both strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone. Both
strains had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysophosphatidylglycerol as the major
polar lipids. Comparative analysis of phenotypic and phylogenetic traits indicated that strains YM2-7T and WD2-19T represented two novel species of the genus Cohnella. The names Cohnella soli sp. nov. (type strain YM2-7T =KACC 13346T =NBRC 106486T), and Cohnella suwonensis sp. nov. (type strain WD2-19T =KACC 13347T =NBRC 106485T) are proposed for these organisms. 相似文献
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A Gram-staining positive, endospore-forming, motile and rod-shaped bacterial strain, BR-29T, was isolated from soil from west coast of the Korean peninsula, and its taxonomic position was investigated by a polyphasic
study. Strain BR-29T grew optimally at around pH 7.5, at 30°C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA
gene sequences showed that strain BR-29T fell into a clade comprising the type strains of Cohnella species, with which it exhibited 16S rRNA gene sequence similarity values of 92.8–96.4%. Strain BR-29T contained a cell wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C15:0, C16:0 and iso-C16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol and two unidentified
phospholipids; a minor amount of phosphatidylglycerol was present. The DNA G+C content was 54.9 mol%. Strain BR-29T could be differentiated from phylogenetically related Cohnella species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain
BR-29T represents a novel species of the genus Cohnella, for which the name Cohnella
boryungensis sp. nov., is proposed. The type strain is BR-29T (= KCTC 13735T = CCUG 59598T). 相似文献
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Yan-yu Meng Yun-bin Fu Meng-yu Wang Yong Zhao Qi-shun Liu Yu-guang Du 《Current microbiology》2013,66(5):487-492
A Gram-negative, aerobic, rod-shaped, motile, non-spore-forming bacterial strain, designated 13-QT, was isolated from seaside soil under the stacks of the red algae in Hainan province in China. Identification was carried out on the basis of polyphasic taxonomy. Phylogenetic analysis of 16S rRNA gene sequences showed that strain 13-QT belonged to the genus Pedobacter, and the highest similarity was 94.4 % with Pedobacter terricola KCTC 12876T. Strain 13-QT was able to grow at 10–40 °C, in pH 5.0–10.0, in the presence of 0–2.0 % NaCl. The major fatty acids were iso-C15:0 (40.4 %), summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c) (18.9 %) and iso-C17:0 3-OH (18.4 %). The predominant menaquinone was MK-7. The G+C content of the genomic DNA was 42.7 mol%. Strain 13-QT could be distinguished from the nearest phylogenetic neighbors by various chemotaxonomic and phenotypic properties. The results of the polyphasic analyses suggested that strain 13-QT should be considered to represent a novel species of the genus Pedobacter, for which the name Pedobacter hainanensis sp. nov. is proposed. The type strain is 13-QT (=CCTCC AB 2012076T = NRRL B-59850T). 相似文献
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The strain designated as AB21T was isolated from chloroethylenes contaminated soil. Cells are gram-negative, aerobic, non-spore-forming, and motile rods. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Rhizobium, and was closely related to Rhizobium sullae IS 123T (97.4 %), Rhizobium yanglingense SH 22623T (97.2 %), Rhizobium gallicum R 602spT (97.1 %), Rhizobium alamii GBV 016T (97.0 %), and Rhizobium monogolense USDA 1844T (97.0 %). It showed less than 97 % identity with the remaining Rhizobium species. This novel isolate grew optimally at 25–37 °C (optimum, 30 °C) and pH 6–9 (optimum, pH 8.0). It grew in the presence of 0–4 % (w/v) NaCl, tolerating a 4 % (w/v) NaCl. DNA–DNA hybridization experiment shows less than 53 % binding with closely related Rhizobium. Predominant quinone is ubiquinone (Q-10). The major fatty acids were summed feature 8 (composed of C18:1 ω7c/C18:1 ω6c), C19:0 cyclo ω8c, and C16:0. The G+C molar content is 62.5 mol%. Based on the polyphasic analysis, strain AB21T is referred to be a novel species of the genus Rhizobium for which the name Rhizobium halotolerans sp. nov. is proposed. The type strain is AB21T (=KEMC 224-056T = JCM 17536T). 相似文献
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Xin-Chi Pan Shuang Geng Ran Mei Ya-Nan Wang Hua Cai Xue-Ying Liu Yue-Qin Tang Yong Nie Si-Yuan Ye Xiao-Lei Wu 《Current microbiology》2014,69(4):561-566
Two Gram-negative, non-motile, short-rod-shaped bacterial isolates, designated 110399T and 110248, were isolated from an oil-polluted saline soil in Shengli Oilfield, Eastern China. The two strains shared 99.9 % 16S rRNA gene sequence similarity with the DNA–DNA relatedness value being 80.0 %. They were both capable to grow at 20–40 °C, pH 7–9, and 1–9 % (w/v) NaCl with the optimum growth happened at 30 °C, pH 8, and 2–6 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains were members of Nitratireductor and most closely related to Nitratireductor pacificus pht-3BT and N. basaltis J3T with the 16S rRNA gene sequence similarities being 97.1 and 97.0 %. The DNA–DNA relatedness between the novel strains and two type strains were below 27 ± 7 %. The strains 110399T and 110248 also differed from N. pacificus and N. basaltis in nitrate reduction, salt tolerance, enzyme activities, and utilization of carbon sources. The major cellular fatty acids of strain 110399T were C19:0ω8c cyclo (10.5 %) and Summed Feature 8 (C18:1ω7c and/or C18:1ω6c, 41.5 %) which are typical in the genus Nitratireductor. The predominant ubiquinone was Q-10. The genome DNA G+C content of strain 110399T and 110248 was 61.1 and 61.7 mol%. On the basis of genetic, phenotypic, and chemotaxonomic analyses, strains 110399T and 110248 represent a novel species within the genus Nitratireductor, for which the name Nitratireductor shengliensis sp. nov. is proposed. The type strain is 110399T (=CGMCC 1.12519T = LMG 27405T). 相似文献
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A Gram-positive, aerobic, rod-shaped, nonmotile, endospore-forming bacterium, designated Gsoil 349T, was isolated from soil of a ginseng field and characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that the strain Gsoil 349T belongs to the family Paenibacillaceae, and the sequence showed closest similarity with Cohnella thermotolerans DSM 17683T (94.1%) and Cohnella hongkongensis DSM 17642T (93.6%). The strain showed less than 91.3% 16S rRNA gene sequence similarity with Paenibacillus species. In addition, the presence of MK-7 as the major menaquinone and anteiso-C(15:0), iso-C(16:0), and C(16:0) as major fatty acids suggested its affiliation to the genus Cohnella. The G+C content of the genomic DNA was 53.4 mol%. On the basis of its phenotypic characteristics and phylogenetic distinctiveness, strain Gsoil 349T should be treated as a novel species within the genus Cohnella for which the name Cohnella panacarvi sp. nov. is proposed. The type strain is Gsoil 349T (=KCTC 13060T = DSM 18696T). 相似文献
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Jae-Jin Lee Sathiyaraj Srinivasan Sangyong Lim Minho Joe Sang Hee Lee Shin Ae Kwon Yoon Jung Kwon Jin Lee Jin Ju Choi Hye Min Lee Young Kyung Auh Myung Kyum Kim 《Current microbiology》2014,68(3):305-310
Gram stain-negative and non-motile bacteria, designated as DY53T and DY43, were isolated from mountain soil in South Korea prior exposure with 5 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strains belonged to the family Cytophagaceae in the class Cytophagia. 16S rRNA gene sequence similarity of strains DY53T and DY43 was 100 %. The highest degrees of sequence similarities of strains DY53T and DY43 were found with Hymenobacter perfusus A1-12T (98.8 %), Hymenobacter rigui WPCB131T (98.5 %), H. yonginensis HMD1010T (97.9 %), H. xinjiangensis X2-1gT (96.6 %), and H. gelipurpurascens Txg1T (96.5 %). The DNA G+C content of the novel strains DY53T and DY43 were 59.5 mol%. Chemotaxonomic data revealed that strains possessed major fatty acids such as C15:0 iso, C15:0 anteiso, C16:1 ω5c, summed feature 3 (16:1 ω7c/ω6c), summed feature 4 (17:1 anteiso B/iso I) and C17:0 iso, and major polar lipid was phosphatidylethanolamine. The novel strains showed resistance to gamma radiation, with a D10 value (i.e., the dose required to reduce the bacterial population by tenfold) in excess of 5 kGy. Based on these data, strains DY53T and DY43 should be classified as representing a novel species, for which the name Hymenobacter swuensis sp. nov. is proposed, with the type strain DY53T (=KCTC 32018T = JCM 18582T) and DY43 (=KCTC 32010). 相似文献
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Yogesh M. Kolekar Shrikant P. Pawar Sunil S. Adav Liu-Qiang Zheng Wen-Jun Li Yogesh S. Shouche Syed G. Dastager Kisan M. Kodam 《Current microbiology》2013,67(4):454-459
A novel Gram-negative, motile, rod-shaped, facultative anaerobic bacterial strain, KMK6T, was isolated from soil contaminated with textile dyes from an industrial estate located at Ichalkaranji, Maharashtra, India, and its taxonomical position was established by using a polyphasic approach. The major cellular fatty acids included C17:1ω8c, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C17:0, C16:0, and C18:1ω7c. The DNA G+C content of strain KMK6T was 48.8 mol %. 16S rRNA gene sequence analysis confirmed its placement in the genus Alishewanella, and exhibited sequence similarity levels of below 97 % to the type strains of validly published Alishewanella species. On the basis of genotypic and phenotypic evidence, strains KMK6T is considered to be a novel species of the genus Alishewanella, for which we propose that strain KMK6T (=NCIM 5295T =BCRC 17848T) is assigned to a novel species, Alishewanella solinquinati sp. nov. 相似文献
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A novel bacterium capable of fixing nitrogen was isolated from plantain rhizosphere soil in China. The isolate, designated YN-83T, is Gram-positive, aerobic, motile and rod-shaped (0.4–0.6 μm × 1.9–2.6 μm). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain YN-83T was a member of the genus Cohnella. High similarity of 16S rRNA gene sequence was found between YN-83T and Cohnella ginsengisoli DSM18997T (97.99%), whereas the similarity was below 96.0% between YN-83T and the other Cohnella species. DNA–DNA relatedness between strain YN-83T and C. ginsengisoli DSM18997T was 27.4 ± 6.2%. The DNA G+C content of strain YN-83T was 59.3 mol%. The predominant isoprenoid quinone was MK-7 and the major fatty acids were anteiso-C15:0 (44.3%), iso-C15:0 (11.3%), iso-C16:0 (18.6%) and C16:0 (7.7%). The polar lipids of strain YN-83T consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lyso- phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. On the basis of phenotypic and chemotaxonomic properties, 16S rRNA gene sequence, G+C content and DNA–DNA hybridization, strain YN-83T represents a novel species of the genus Cohnella, for which the name Cohnella plantaginis sp. nov. (type strain YN-83T = DSM 25424T = CGMCC 1.12047T) is proposed. 相似文献
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Jae-Jin Lee Sathiyaraj Srinivasan Sangyong Lim Minho Joe Seonghun Im So Il Bae Kyoung Ryun Park Ji-Hee Han Se-Hee Park Bo-min Joo Sol-Ji Park Myung Kyum Kim 《Current microbiology》2014,69(3):286-291
A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5AT, was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5AT belong to the genus Spirosoma in the family Spirosomaceae, and the highest sequence similarities were 95.5 % with Spirosoma linguale DSM 74T, 93.4 % with Spirosoma rigui WPCB118T, 92.8 % with Spirosoma luteum SPM-10T, 92.7 % with Spirosoma spitsbergense SPM-9T, and 91.9 % with Spirosoma panaciterrae Gsoil 1519T. Strain DG5AT revealed resistance to gamma and UV radiation. Chemotaxonomic data showed that the most abundant fatty acids were summed feature C16:1 ω7c/C16:1 ω6c (36.90 %), C16:1 ω5c (29.55 %), and iso-C15:0 (14.78 %), and the major polar lipid was phosphatidylethanolamine (PE). The DNA G+C content of strain DG5AT was 49.1 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain DG5AT presents a novel species of the genus Spirosoma, for which the name Spirosoma radiotolerans sp. nov., is proposed. The type strain is DG5AT (=KCTC 32455T = JCM19447T). 相似文献
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A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5T, was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5T belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979T (98.4 %) and Deinococcus daejeonensis KCTC 13751T (97.5 %). Strain N5T exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C16:1 ω7c (25.25 %), C15:1 ω6c (19.77 %), C17:1 ω6c (11.87 %), and C17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5T as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. nov. is proposed. The type strain is N5T (=KCTC 33153T = JCM 19176T). 相似文献