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1.
Piao ZY Deng YQ Choi SR Park YJ Lim YP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(8):1458-1465
Clubroot disease, caused by Plasmodiophora brassicae Wor., is highly damaging for Chinese cabbage. The CR (clubroot resistant) Shinki DH (doubled haploid) line of Chinese cabbage carries a single dominant gene, CRb, which confers resistance to the P. brassicae races 2, 4, and 8. An F2 population derived from a cross between the CR Shinki DH line and a susceptible line, 94SK, was used to map the CRb gene. Inoculation of F3 families with SSI (single-spore isolate) resulted in a 1:2:1 segregation ratio. Use of the AFLP technique combined with bulked segregant analysis allowed five co-dominant AFLP markers, and four and seven dominant AFLP markers linked in coupling and repulsion, respectively, to be identified. Six of the 16 AFLP markers showing low frequencies of recombination with the CRb locus among 138 F2 lines were cloned. A reliable conversion procedure allowed five AFLP markers to be successfully converted into CAPS and SCAR markers. An F2 population (143 plants) was analyzed with these markers and a previously identified SCAR marker, and a genetic map around CRb covering a total distance of 6.75 cM was constructed. One dominant marker, TCR09, was located 0.78 cM from CRb. The remaining markers (TCR05, TCR01, TCR10, TCR08, and TCR03) were located on the other side of CRb, and the nearest of these was TCR05, at a distance of 1.92 cM.Communicated by R. Hagemann 相似文献
2.
Sabharwal V Negi MS Banga SS Lakshmikumaran M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(1):160-166
Association mapping of the seed-coat colour with amplified fragment length polymorphism (AFLP) markers was carried out in 39 Brassica juncea lines. The lines had genetically diverse parentages and varied for seed-coat colour and other morphological characters. Eleven AFLP primer combinations were used to screen the 39 B. juncea lines, and a total of 335 polymorphic bands were detected. The bands were analysed for association with seed-coat colour using multiple regression analysis. This analysis revealed 15 markers associated with seed-coat colour, obtained with eight AFLP primer combinations. The marker E-ACA/M-CTG350 explained 69% of the variation in seed-coat colour. This marker along with markers E-AAC/M-CTC235 and E-AAC/M-CTA250 explained 89% of the total variation. The 15 associated markers were validated for linkage with the seed-coat colour loci using a recombinant inbred line (RIL) mapping population. Bands were amplified with the eight AFLP primer combinations in 54 RIL progenies. Of the 15 associated markers, 11 mapped on two linkage groups. Eight markers were placed on linkage group 1 at a marker density of 6.0 cM, while the remaining three were mapped on linkage group 2 at a marker density of 3.6 cM. Marker E-ACA/M-CTG350 co-segregated with Gene1 controlling seed-coat colour; it was specific for yellow seed-coat colour and mapped to linkage group 1. Marker E-AAC/M-CTC235 (AFLP8), which had been studied previously, was present on linkage group 2; it was specific for brown seed-coat colour. Since AFLP markers are not adapted for large-scale applications in plant breeding, it is important to convert these to sequence-characterised amplified region (SCAR) markers. Marker E-AAC/M-CTC235 (AFLP8) had been previously converted into a SCAR. Work is in progress to convert the second of the linked markers, E-ACA/M-CTG350, to a SCAR. The two linked AFLP markers converted to SCARs will be useful for developing yellow-seeded B. juncea lines by means of marker-assisted selection.Communicated by H.F. Linskens 相似文献
3.
Zhou C Chen C Cao P Wu S Sun J Jin D Wang B 《Molecular genetics and genomics : MGG》2007,278(6):723-728
Southern corn rust (SCR) is a fungal disease caused by Puccinia polysora Underw, which can infect maize and may result in substantial yield losses in maize production. The maize inbred line Qi319
carries the SCR resistance gene RppQ. In order to identify molecular markers linked to the RppQ gene, several techniques were utilized including random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and
amplified fragment length polymorphism (AFLP). In addition, sequence characterized amplified region (SCAR) techniques combined
with bulked segregant analysis (BSA) were used. Seven RAPD markers, eight SSR markers, and sixty-three AFLP primer combinations
amplified polymorphisms between two parents and two bulk populations. A large F2 population was used for genetic analysis and for fine mapping of the RppQ gene region. One AFLP polymorphic band, M-CAA/E-AGC324, was converted to a SCAR marker, MA7, which was mapped to a position 0.46 cM from RppQ. Finally, the RppQ gene was mapped between the SCAR marker MA7 and the AFLP marker M-CCG/E-AGA157 with distances of 0.46 and 1.71 cM, respectively. 相似文献
4.
Mienie CM Liebenberg MM Pretorius ZA Miklas PN 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(5):972-979
Rust in common bean (Phaseolus vulgaris L.) is caused by Uromyces appendiculatus Pers.:Pers. (Unger) which exhibits a high level of pathogenic diversity. Resistance to this disease is conditioned by a considerable
number of genes. Pyramiding resistance genes is desirable and could be simplified by the use of molecular markers closely
linked to the genes. The resistance gene Ur-13, present in the South African large seeded cultivar Kranskop, has been used extensively in the local breeding program. The
purpose of this study was the development of a molecular marker linked to Ur-13. An F2 population derived from a cross between Kranskop and a susceptible (South African) cultivar Bonus was used in combination
with bulked segregant analysis utilizing the amplified fragment length polymorphism (AFLP) technique. Seven AFLP fragments
linked significantly to the rust resistance and five were successfully converted to sequence characterized amplified region
(SCAR) markers. The co-dominant SCAR markers derived from a 405 bp EAACMACC fragment, KB126, was located 1.6 cM from the gene.
Two additional SCAR markers and one cleaved amplified polymorphic sequence marker were located further from the gene. The
gene was mapped to linkage group B8 on the BAT 93/Jalo EEP 558 core map (chromosome 3). 相似文献
5.
Fernando J. Yuste-Lisbona Carmen Capel Emilio Sarria Rocío Torreblanca María L. Gómez-Guillamón Juan Capel Rafael Lozano Ana I. López-Sesé 《Molecular breeding : new strategies in plant improvement》2011,27(2):181-192
Powdery mildew caused by Podosphaera xanthii has become a major problem in melon since it occurs all year round irrespective of the growing system. The TGR-1551 melon
genotype was found to be resistant to several melon diseases, among them powdery mildew. However, the corresponding resistance
genes have been never mapped. We constructed an integrated genetic linkage map using an F2 population derived from a cross
between the multi-resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’. The map spans 1,284.9 cM,
with an average distance of 3.6 cM among markers, and consists of 354 loci (188 AFLP, 39 RAPD, 111 SSR, 14 SCAR/CAPS/dCAPS,
and two phenotypic traits) distributed in 14 linkage groups. QTL analysis identified one major QTL (Pm-R) on LG V for resistance to races 1, 2, and 5 of powdery mildew. The PM4-CAPS marker is closely linked to the Pm-R QTL at a genetic distance of 1.9 cM, and the PM3-CAPS marker is located within the support interval of this QTL. These codominant
markers, together with the map information reported here, could be used for melon breeding, and particularly for genotyping
selection of resistance to powdery mildew in this vegetable crop species. 相似文献
6.
Y. H. Lu J. M. Melero-Vara J. A. García-Tejada P. Blanchard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):625-632
A consensus molecular linkage map of 61.9 cM containing the Or5 gene, which confers resistance to race E of broomrape orobanche cumana, five SCAR markers (three dominant, two codominant) and one RAPD marker were identified based on segregation data scored
from two F2 populations of susceptible×resistant sunflower line crosses. Bulked segregant analysis was carried out to generate the five
SCAR markers, while the single RAPD marker in the group was identified from 61 segregating RAPD markers that were directly
screened on one of the two F2 populations. The five SCAR markers, RTS05, RTS28, RTS40, RTS29 and RTS41, were significantly (LOD≥4.0) linked to the Or5 gene and mapped separately at 5.6, 13.6, 14.1, 21.4 and 39.4 cM from the Or5 locus on one side, while the RAPD marker, UBC120_660, was found at 22.5 cM (LOD=1.4) on the opposite side. These markers
should facilitate the efficient transfer of the resistance gene among sunflower breeding lines. As the first report on molecular
markers linked to a broomrape resistance gene, the present work provides a starting point to study other genes and to examine
the hypothesis of the clustering of broomrape resistance genes in sunflower.
Received: 16 September 1998 / Accepted: 22 June 1999 相似文献
7.
Lawson W.R. Goulter K.C. Henry R.J. Kong G.A. Kochman J.K. 《Molecular breeding : new strategies in plant improvement》1998,4(3):227-234
In this study we report on the identification of molecular markers, OX20600 and OO04950, linked to the geneR
Adv in the proprietary inbred line P2. This gene confers resistance to most of the pathotypes of Puccinia helianthi identified in Australia. Analysis indicates these RAPD markers are linked to the resistance locus at 0.0 cM and 11 cM respectively. SCAR markers SCX20600 and SCO04950 derived from these two RAPD markers, and SCT06950 derived from a previously reported RAPD marker linked at 4.5 cM from the R
1 rust resistance gene were developed. SCX20600 and SCO04950 were linked at similar distances from their resistance locus as the RAPD markers. SCTO6950 co-segregated completely with rust resistance. The robustness of the R
1 SCAR marker was demonstrated through the amplification of the marker in a diverse range of sunflower germplasm considered to possess the R
1 gene. The SCAR markers forR
Adv were not amplified in the sunflower rust differential set thereby supporting the contention that this is a novel resistance gene. They did amplify in a number of proprietary lines closely related to the line P2. This locus is under further investigation as it will be useful in our attempts to use molecular-assisted breeding to produce durable resistance in sunflower to P. helianthi. 相似文献
8.
Ashutosh Sharma PC Prakash S Bhat SR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(2):385-392
We have developed a cytoplasmic male sterile (CMS) line of Brassica juncea through somatic hybridization with Moricandia arvensis and introgressed the fertility restorer gene into B. juncea. This fertility restorer locus is unique in that it is capable of restoring male fertility to two other alloplasmic CMS systems
of B. juncea. As a first step toward cloning of this restorer gene we attempted molecular tagging of the Rf locus using the amplified fragment length polymorphism (AFLP) technique. A BC1F1 population segregating for male sterility/fertility was used for tagging using the bulk segregant analysis method. Out of
64 primer combinations tested in the bulks, 5 combinations gave polymorphic amplification patterns. Further testing of these
primers in individual plants showed four amplicons associated with the male fertility trait. Polymorphic amplicons were cloned
and used for designing SCAR primers. One of the SCAR primers generated amplicons mostly in the fertile plants. Linkage analysis
using MAPMAKER showed two AFLP and one SCAR markers linked to the male fertility gene with a map distance ranging from 0.6
to 2.9 cM. All the markers are located on one side of the Rf locus. 相似文献
9.
Hui Feng Peng Wei Zhong-Yun Piao Zhi-Yong Liu Cheng-Yu Li Yu-Gang Wang Rui-Qin Ji Shu-Juan Ji Ting Zou Su-Ryun Choi Yong-Pyo Lim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(2):333-339
The genic multiple-allele inherited male-sterile gene Ms in Chinese cabbage (Brassica rapa L.) was identified as a spontaneous mutation. Applying this gene to hybrid seed production, several B. rapa cultivars have been successfully bred in China. A BC1 population (244 plants) was constructed for mapping the Ms gene. Screening 268 simple sequence repeat (SSR) markers which cover the entire genome of Chinese cabbage was performed with
bulked segregant analysis (BSA). On the basis of linkage analysis, the Ms gene was located on linkage group R07. In addition, through the amplified fragment length polymorphism (AFLP) and the sequence-characterized
amplified region (SCAR) techniques combining BSA, two SCAR markers which were converted from corresponding AFLP markers flanked
the Ms gene. Finally, a genetic map of the Ms gene was constructed covering a total interval of 9.0 cM. Two SCAR markers, syau_scr01 and syau_scr04, flanked the Ms gene at distances of 0.8 and 2.5 cM, respectively. All the SSR markers (cnu_m273, cnu_m030, cnu_m295, and syau_m13) were
mapped on the same side of the gene as syau_scr04, the nearest one of which, syau_m13, was mapped at a distance of 3.3 cM.
These SSR and SCAR markers may be useful in marker-assisted selection and map-based cloning.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
10.
Molecular mapping of <Emphasis Type="Italic">Stb1</Emphasis>, a potentially durable gene for resistance to septoria tritici blotch in wheat 总被引:1,自引:0,他引:1
Adhikari TB Yang X Cavaletto JR Hu X Buechley G Ohm HW Shaner G Goodwin SB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(5):944-953
Septoria tritici blotch (STB), caused by the ascomycete Mycosphaerella graminicola (anamorph Septoria tritici), was the most destructive disease of wheat in Indiana and adjacent states before deployment of the resistance gene Stb1 during the early 1970s. Since then, Stb1 has provided durable protection against STB in widely grown wheat cultivars. However, its chromosomal location and allelic relationships to most other STB genes are not known, so the molecular mapping of Stb1 is of great interest. Genetic analyses and molecular mapping were performed for two mapping populations. A total of 148 F1 plants (mapping population I) were derived from a three-way cross between the resistant line P881072-75-1 and the susceptible lines P881072-75-2 and Monon, and 106 F6 recombinant-inbred lines (mapping population II) were developed from a cross between the resistant line 72626E2-12-9-1 and the susceptible cultivar Arthur. Bulked-segregant analysis with random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and microsatellite or simple-sequence repeat (SSR) markers was conducted to identify those that were putatively linked to the Stb1 gene. Segregation analyses confirmed that a single dominant gene controls the resistance to M. graminicola in each mapping population. Two RAPD markers, G71200 and H19520, were tightly linked to Stb1 in wheat line P881072-75-1 at distances of less than 0.68 cM and 1.4 cM, respectively. In mapping population II, the most closely linked marker was SSR Xbarc74, which was 2.8 cM proximal to Stb1 on chromosome 5BL. Microsatellite loci Xgwm335 and Xgwm213 also were proximal to Stb1 at distances of 7.4 cM and 8.3 cM, respectively. The flanking AFLP marker, EcoRI-AGC/MseI-CTA-1, was 8.4 cM distal to Stb1. The two RAPD markers, G71200 and H19520, and AFLP EcoRI-AGC/MseI-CTA-1, were cloned and sequenced for conversion into sequence-characterized amplified region (SCAR) markers. Only RAPD allele H19520 could be converted successfully, and none of the SCAR markers was diagnostic for the Stb1 locus. Analysis of SSR and the original RAPD primers on several 5BL deletion stocks positioned the Stb1 locus in the region delineated by chromosome breakpoints at fraction lengths 0.59 and 0.75. The molecular markers tightly linked to Stb1 could be useful for marker-assisted selection and for pyramiding of Stb1 with other genes for resistance to M. graminicola in wheat. 相似文献
11.
Aixia Xu Zhen Huang Chaozhi Ma Enshi Xiao Guangwen Tian Xiusen Zhang Jinxing Tu Tingdong Fu Gaisheng Zhang 《Molecular breeding : new strategies in plant improvement》2010,25(1):57-65
Seed color inheritance in Brassica juncea was studied in F1, F2 and BC1 populations. Seed color was found under the control of the maternal genotype, and the brown-seeded trait was dominant over
the yellow-seeded trait. Segregation analysis revealed that one pair of major genes controlled the seed coat color. To develop
markers linked to the seed color gene, AFLP (amplified fragments length polymorphism) combined with BSA (bulk segregant analysis)
technology was used to screen the parents and bulks selected randomly from an F2 population (Wuqi yellow mustard × Wugong mustard) consisting of 346 individuals. From a survey of 512 AFLP primer combinations,
15 AFLP markers located on either side of the gene were identified, and the average distance between markers was 2.59 cM.
P11MG15 was a cosegregated marker, and the closest markers (P03MC08, P16MC02 and P11MG01) were at a distance of 0.3, 0.3 and
0.7 cM from the target gene, respectively. In order to utilize the markers for breeding of yellow-seeded varieties, four AFLP
markers, P11MG01, P15MG15, P09MC12 and P16MC02 were successfully converted into SCAR (sequence characterized amplified region)
markers. The seed color trait controlled by the single gene together with the available molecular markers will greatly facilitate
the future breeding of yellow-seeded varieties. The markers found in the present study could accelerate the step of map-based
cloning of the target gene. 相似文献
12.
Singh M Chaudhary K Boora KS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,114(1):187-192
Anthracnose, caused by Colletotrichum graminicola, infects all aerial parts of sorghum, Sorghum bicolor (L.) Moench, plants and causes loss of as much as 70%. F1 and F2 plants inoculated with local isolates of C. graminicola indicated that resistance to anthracnose in sorghum accession G 73 segregated as a recessive trait in a cross with susceptible cultivar HC 136. To facilitate the use of marker-assisted selection in sorghum breeding programs, a PCR-based specific sequence characterized amplified region (SCAR) marker was developed. A total of 29 resistant and 20 susceptible recombinant inbred lines (RILs) derived from a HC 136 × G 73 cross was used for bulked segregant analysis to identify a RAPD marker closely linked to a gene for resistance to anthracnose. The polymorphism between the parents HC 136 and G 73 was evaluated using 84 random sequence decamer primers. Among these, only 24 primers generated polymorphism. On bulked segregant analysis, primer OPA 12 amplified a unique band of 383 bp only in the resistant parent G 73 and resistant bulk. Segregation analysis of individual RILs showed the marker OPA 12383 was 6.03 cM from the locus governing resistance to anthracnose. The marker OPA 12383 was cloned and sequenced. Based on the sequence of cloned RAPD product, a pair of SCAR markers SCA 12-1 and SCA 12-2 was designed using the MacVector program, which specifically amplified this RAPD fragment in resistant parent G 73, resistant bulk and respective RILs. Therefore, it was confirmed that SCAR marker SCA 12 is at the same locus as RAPD marker OPA 12383 and hence, is linked to the gene for resistance to anthracnose. 相似文献
13.
Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice 总被引:9,自引:0,他引:9
J. H. Jia D. S. Zhang C. Y. Li X. P. Qu S. W. Wang V. Chamarerk H. T. Nguyen B. Wang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):607-612
TGMS (thermo-sensitive genic male-sterile) rice is widely used in hybrid rice production. Because of a specific temperature
requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered reverse thermo-sensitive genic
male-sterile line, J207S, has an opposite phynotype compared to the normal TGMS lines. J207S is completely sterile when the
temperature is lower than 31°C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility
of J207S was controlled by a single recessive gene which was first named as rtms1. An F2 population from the cross between J207S and E921 was developed and used for molecular mapping of the rtms1 gene. The AFLP (amplified fragment length polymorphism) technique, combined with BSA (bulked segregant analysis), was used
to screen markers linked to the target gene, and eight polymorphic AFLP loci were identified. Co-segregating analysis using
the F2 population showed that two of them, Rev1 and Rev7, were closely linked to the target gene with a recombinant rate of 3.8%
and 7.7%, respectively. Both Rev1 and Rev7 were found to be single-copy sequences through Southern analysis. Rev1 was subsequently
mapped on chromosome 10 with a doubled-haploid mapping populations derived from the cross CT9993 × IR62266 available at Texas
Tech University. RM222 and RG257 were linked to Rev1 at a distance of 11.8 cM and 4.6 cM, respectively. Additional SSR markers
from the rice map of Cornell University, RFLP markers from the map of RGP in Japan and the map of Texas Tech University were
selected from the region surrounding Rev1 on chromosome 10 to conduct the fine-mapping of the rtms1 gene. Presently, rtms1 was mapped between RM239 and RG257 with genetic distance of 3.6 cM and 4.0 cM, respectively. The most-closely linked AFLP
marker, Rev1, 4.2 cM from the rtms1 gene, was sequenced and converted into a SCAR (sequence characterized amplified region) marker which could facilitate marker-assisted
selection of the rtms1 gene.
Received: 2 November 2000 / Accepted: 21 November 2000 相似文献
14.
Velásquez AC Mihovilovich E Bonierbale M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(6):1051-1058
Major gene inheritance of resistance to Potato leafroll virus (PLRV) was demonstrated in a parthenogenic population derived
from the highly resistant tetraploid andigena landrace, LOP-868. This major gene or chromosome region seems to control a single mechanism for resistance to infection and
virus accumulation in this source. About 149 dihaploid lines segregated in a ratio of 107 resistant to 32 susceptible, fitting
the expected ratio for inheritance of a duplex gene under random chromatid segregation. A tetraploid AFLP map was constructed
using as reference the ultra high density (UHD) map. All AFLP markers associated with PLRV resistance mapped to the same linkage
group. Map position was confirmed by analysis of previously-mapped SSR markers. Rl
adg is located on the upper arm of chromosome V, at 1 cM from its most closely linked AFLP marker, E35M48.192. This marker will be used to develop allele-specific primers
or a pair of flanking PCR-based markers for their use in marker assisted selection. 相似文献
15.
B. von Malek W. E Weber T. Debener 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):977-983
Blackspot resistance in the tetraploid rose genotype 91/100–5 had been characterised previously as a single dominant gene
in duplex configuration. In the present study a tetraploid progeny (95/3) segregating for the presence of the blackspot resistance
gene Rdr1 were screened with 868 RAPD and 114 AFLP primers/primer combinations. Seven AFLP markers were found to be linked to Rdr1 at distances between 1.1 and 7.6 cM. The most closely linked AFLP marker was cloned and converted into a SCAR marker that
could be screened in a larger population than the original AFLP and was linked at a distance of 0.76 cM. The cloned fragment
was used as an RFLP probe to locate the marker on a chromosome map of diploid roses. This is the first report of markers linked
to a resistance gene in roses, and the possibilities of using them for a marker-assisted selection for blackspot resistance
as well as for map-based cloning approaches are discussed.
Received: 23 December 1999 / Accepted: 25 March 2000 相似文献
16.
AFLP and PCR-based markers linked to Rf3, a fertility restorer gene for S cytoplasmic male sterility in maize 总被引:2,自引:0,他引:2
The Rf3 gene restores the pollen fertility disturbed by S male sterile cytoplasm. In order to develop molecular markers tightly linked to Rf3, we used amplified fragment length polymorphism (AFLP) technique with near isogenic lines (NILs) and bulk segregant analysis (BSA). A BC1F1 population from a pair of NILs with different Rf3 locus was constructed and 528 primer combinations was screened. A linkage map was constructed around the Rf3 locus, which was mapped on the distal region of chromosome 2 long arm with the help of SSR marker UMC2184. The closest marker E7P6 was 0.9 cM away from Rf3. Marker E3P1, 2.4 cM from Rf3, and E12M7, 1.8 cM from Rf3, were converted into a codominant CAPS and a dominant SCAR marker, and designated as CAPSE3P1 and SCARE12M7, respectively. These markers are useful for marker-assisted selection and map-based cloning of the Rf3 gene. 相似文献
17.
Molecular tagging and mapping of the erect panicle gene in rice 总被引:6,自引:0,他引:6
Fan Na Kong Jia Yu Wang Ji Cheng Zou Li Xue Shi Min De Jin Zheng Jin Xu Bin Wang 《Molecular breeding : new strategies in plant improvement》2007,19(4):297-304
Erect panicle (EP) is one of the more important traits of the proposed ideotype of high-yielding rice. Several rice cultivars
with the EP phenotype, which has been reported to be controlled by a dominant gene, have been successfully developed and released
for commercial production in North China. To analyze the inheritance of the EP trait, we generated segregating F2 and BC1F1 populations by crossing an EP-type variety, Liaojing 5, and a curved-panicle-type variety, Fengjin. Our results confirmed that a dominant gene controls the EP trait. Simple-sequence repeat (SSR) and bulked segregant analyses
of the F2 population revealed that the EP gene is located on chromosome 9, between two newly developed SSR markers, RM5833-11 and RM5686-23, at a genetic distance
of 1.5 and 0.9 cM, respectively. Markers closer to the EP gene were developed by amplified fragment length polymorphism (AFLP) analysis with 128 AFLP primer combinations. Three AFLP
markers were found to be linked to the EP gene, and the nearest marker, E-TA/M-CTC200, was mapped to the same location as SSR marker RM5686-23, 1.5 cM from the EP gene. A local map around the EP gene comprising nine SSR and one AFLP marker was constructed. These markers will be useful for marker-assisted selection
(MAS) for the EP trait in rice breeding programs. 相似文献
18.
Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant 总被引:1,自引:0,他引:1
Mutlu N Boyaci FH Göçmen M Abak K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(8):1303-1312
Fusarium wilt (Fusarium oxysporum Schlecht. f. sp. melongenae) is a vascular disease of eggplant (Solanum melongena L.). The objectives of this work were (1) to confirm the monogenic inheritance of fusarium wilt resistance in eggplant, (2)
to identify molecular markers linked to this resistance, and (3) to develop SCAR markers from most informative markers. We
report the tagging of the gene for resistance to fusarium wilt (FOM) in eggplant using SRAP, RGA, SRAP-RGA and RAPD markers.
Analysis of segregation data confirmed the monogenic inheritance of resistance. DNA from F2 and BC1 populations of eggplant segregating for fusarium wilt resistance was screened with 2,316 primer combinations to detect polymorphism.
Three markers were linked within 2.6 cM of the gene. The codominant SRAP marker Me8/Em5 and dominant SRAP-RGA marker Em12/GLPL2
were tightly linked to each other and mapped 1.2 cM from the resistance gene, whereas RAPD marker H12 mapped 2.6 cM from the
gene and on the same side as the other two markers. The SRAP marker was converted into two dominant SCAR markers that were
confirmed to be linked to the resistance gene in the F2, BC1 and F2 of BC3 generations of the same cross. These markers provide a starting point for mapping the eggplant FOM resistance gene in eggplant
and for exploring the synteny between solanaceous crops for fusarium wilt resistance genes. The SCAR markers will be useful
for identifying fusarium wilt-resistant genotypes in marker-assisted selection breeding programs using segregating progenies
of the resistant eggplant progenitor used in this study. 相似文献
19.
A physical map covering the nsv locus that confers resistance to Melon necrotic spot virus in melon (Cucumis melo L.) 总被引:1,自引:0,他引:1
Morales M Orjeda G Nieto C van Leeuwen H Monfort A Charpentier M Caboche M Arús P Puigdomènech P Aranda MA Dogimont C Bendahmane A Garcia-Mas J 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(5):914-922
Melon necrotic spot virus (MNSV) is a member of the genus Carmovirus, which produces severe yield losses in melon and cucumber crops. The nsv gene is the only known natural source of resistance against MNSV in melon, and confers protection against all widespread
strains of this virus. nsv has been previously mapped in melon linkage group 11, in a region spanning 5.9 cM, saturated with RAPD and AFLP markers.
To identify the nsv gene by positional cloning, we started construction of a high-resolution map for this locus. On the basis of the two mapping
populations, F2 and BC1, which share the same resistant parent PI 161375 (nsv/nsv), and using more than 3,000 offspring, a high-resolution genetic map has been constructed in the region around the nsv locus, spanning 3.2 cM between CAPS markers M29 and M132. The availability of two melon BAC libraries allowed for screening
and the identification of new markers closer to the resistance gene, by means of BAC-end sequencing and mapping. We constructed
a BAC contig in this region and identified the marker 52K20sp6, which co-segregates with nsv in 408 F2 and 2.727 BC1 individuals in both mapping populations. We also identified a single 100 kb BAC that physically contains the
resistance gene and covers a genetic distance of 0.73 cM between both BAC ends. These are the basis for the isolation of the
nsv recessive-resistance gene. 相似文献
20.
Lei S Yao X Yi B Chen W Ma C Tu J Fu T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):643-651
S45AB, a recessive genic male sterile (RGMS) line, originated as a spontaneous mutant in Brassica napus cv. Oro. The genotypes of sterile (S45A) and fertile plants (S45B) are Bnms1ms1ms2ms2 and BnMs1ms1ms2ms2, respectively. In our previous studies, Yi et al. (Theor Appl Genet 113:643–650, 2006) mapped the BnMs1 locus to a region of 0.4 cM, candidates of which have been identified and genetic transformation is in progress. We describe
the fine mapping of BnMs2 exploiting amplified fragment length polymorphism (AFLP) and amplified consensus genetic marker (ACGM) methodologies, and
the identification of a collinear region probably containing BnMs2 orthologue in Arabidopsis
thaliana. A near isogenic line (NIL) population S4516AB which segregated for BnMs2 locus was generated by crossing, allelism testing and repeated full-sib mating. From the survey of 1,024 AFLP primer combinations,
12 tightly linked AFLP markers were obtained and five of them were successfully converted into co-dominant or dominant sequence
characterized amplified region (SCAR) markers. A population of 2,650 sterile plants was screened using these markers and a
high-resolution map surrounding BnMs2 was constructed. The closest AFLP markers flanking BnMs2 were 0.038 and 0.075 cM away, respectively. Subsequently, an ACGM marker was developed to delimit the BnMs2 locus at an interval of 0.075 cM. We extended marker sequences to perform BlastN searches against the Arabidopsis genome and identified a collinear region containing 68 Arabidopsis genes, in which the orthologue of BnMs2 might be included. We further integrated BnMs2 linked AFLP or SCAR markers to two doubled-haploid (DH) populations derived from the crosses Tapidor × Ningyou7 (Qiu et al.,
Theor Appl Genet 114:67–80, 2006) and Quantum × No.2127-17 (available in our laboratory), and BnMs2 was mapped on N16. Molecular markers developed from these investigations will facilitate the marker-assisted selection (MAS)
of RGMS lines, and the fine map and syntenic region identified will greatly hasten the process of positional cloning of BnMs2 gene. 相似文献